OBJECTIVE:To determine the content of the total flavonoid in Fructus Rosae Laevigatae.METHODS:Dual wavelength TLC scanner was selected with rutin as standard substance.The developing solvent was acetic ether-formic acid-water(8∶1∶1);?s=266 nm,and ?R=230 nm.RESULTS:The linear range of Fructus Rosae Laevigatae was 1～6 ?g,and the average recovery was 98.9% 2.397%(RSD=1.39%,n=6).CONCLUSION:The method is simple and accurate,and it is applicable for the quality control of Fructus Rosae Laevigatae.

Open ocular trauma in children is one of the main causes of non-congenital monocular blindness in children. Children’s psychological and daily activities are different from those of adults; the eye tissue structure is not maturely enough, and the characteristics of high response to injury and incompatibility with various diagnostic and treatment methods determine the factors that cause injuries and injuries in children with open ocular trauma. The environment, diagnosis and treatment, and prognosis of children are more complicated than adults. For the emergency of first and second stage on diagnosis and treatment of children with open ocular trauma, it should follow the principles of adult open ocular trauma management, combined with the children's characteristics, clarifying the anatomical location of the ocular trauma before surgery, and closing the wound during the first-stage operation. At the same time, the disturbance of intraocular tissue should be minimized, the timing of the second stage operation and the individualization of the operation design, and the medication and care after the operation should also be combined with the characteristics of children. Prevention of ocular trauma in children is the focus of attention. In the new information era, clinical research and public information platforms should be fully utilized to advance the epidemiological study of children's ocular trauma and guide the construction of the prevention and treatment system for children's ocular trauma more scientifically.

AIM To establish a RP-HPLC method for the simultaneous content determination of four constituents in Canarii Fructus.METHODS The analysis of Canarii Fructus methanol extract was performed on a 30 ℃ thermostatic Hypersil ODS column (4.6 mm × 200 mm,5 μm),with the mobile phase comprising of acetonitrile0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 237 nm.RESULTS Gallic acid,protocatechuic acid,chlorogenic acid and epicatechin displayed good linear relationships within the ranges of 0.525-10.500 μg/mL (r =0.999 9),0.700-14.000 μg/mL (r =0.999 9),0.575-11.500 μg/mL (r =0.999 9) and 0.550-11.000 μg/mL (r =0.999 9),whose average recoveries were 99.05％ (RSD=1.4％),98.73％ (RSD=0.8％),98.69％ (RSD=1.3％) and 99.47％ (RSD=1.1％),respectively.CONCLUSION This simple,sensitive and relible method can be used for the quality control of Canarii Fructus.

0.05),as well as in the composite of MACCE(0.47% vs 1.14%) between the two groups.Also,no significant difference existed in the rate of target vessel revascularization(3.48% vs 3.28%,P=0.958),occlusion of lower extremity artery(0.67% vs 0.82%,P=0.857) and hemorrhagic events(0.67% vs 0.82%,P=0.613) between the two groups.Kaplan-Meier survival analysis showed the cumulative hazard rate for the MACCE was similar(P=0.521).There were 5 and 4 CR patients in the Plavix group and the clopidogrel bisulfate group,respectively.Dose enlargement in addition to dual antiplatelet therapy decreased significantly the PAR of CR patients in both groups(78.2%?11.9%,76.2%?10.5%,vs 80.8%?13.2%,81.8%?12.2%,P

OBJECTIVE:To establish and compare the HPLC characteristic Spectrum Erycibe obtusifolia from different habi-tats. METHODS:HPLC was performed on the column of Hypersil ODS C18 with the mobile phase of acetonitrile-0.1% phosphoric acid at flow rate of 1.0 ml/min,detection wavelength was 347 nm,the column temperature was 30℃and the sample size was 20μl. 12 different batches of samples were determined and the characteristic spectrums of those were established. TCM fingerprint evalu-ation software(2004 edition)was used to evaluate the similarity of 12 batches of samples. RESULTS:10 characteristic peaks were identified in the characteristic spectrum of E. obtusifolia. Peak 3 was scopolamine glycosides,peak 4 was chlorogenic acid and peak 6 was scopoletin. The similarities of 10 batches of samples were proved to be higher than 0.90 and 2 batches were proved to be less than 0.90. CONCLUSIONS:The method is simple,accurate and reproducible,and can provide scientific evidence for effectively controlling the internal quality standards.

Objective To study the apo-E gene polymorphisms and the lipoprotein level character of different gene expression in patients with cerebrovascular infarction(CI) and intracerebral hemorrhage(ICH).Methord With case-control study method,the apo-E gene expression,its allele frequency and the levels of serum lipids in patients with cerebrovascular infarction or with intracerebral hemorrhage and the control were compared.Result The CI group had a higher apo-E ?4 allele frequency(10%) than controls(4.6%)(P

BACKGROUND: The existing technique takes articular process and lateral mass as a reference mark. Due to differences of subjective judgment in operation and the impact of articular hyperplasia, some errors of screw entry point selection and serious complications such as vertebral artery or cervical spinal cord injury easily occurred, which limit the clinical application of the technique. At present, it is very important to select a constant reference mark of cervical pedicle screw entry point.OBJECTIVE: Three-dimensional reconstruction CT images were made to measure the relationship between the lower cervical pedicle screw entry point and the lateral concave between articular process.METHODS: Three-dimensional reconstruction of CT scan was performed in 30 patients with cervical deformity, and the occurrence rate and morphological characteristics of the lateral concave between articular process were observed. The following parameters were determined on specific reconstructed CT image of C3-C7: (1) the distance between pedicle axis projection point in the posterior surface and the outer edge of lateral mass in transversal section through bilateral pedicle axis and (2) the distance between the point and the lateral concave between articular process in oblique sagittal section through homolateral pedicle axis. Mean value and standard deviation were counted and statistics difference was compared.RESULTS AND CONCLUSION: (1) The lateral concave between articular process in lower cervical vertebrae was a obvious and less constant proliferative anatomical landmark and its occurrence rate was 100%. (2) Using CT technology of 3D reconstruction, C3-C7 lower cervical transversal section through bilateral pedicle axis and oblique sagittal section through homolateral pedicle axis were successfully obtained. (3) In transversal section, the left and right distances between C3-C7 pedicle axis projection point in the posterior surface and the outer edge of lateral mass were (4.1±0.9)mm and (4.3±0.9) mm, and the difference was not statistically significant (P=0.609). Except for C3 and C7, C4 and C7,there was no significant difference in the measured values of the same side (P > 0.05). (4) In oblique sagittal section, the left and right distances between C3-C7 point and the lateral concave between articular process were (-0.3±1.7) mm and (-0.3±1.6) mm, and the difference was not statistically significant (P=0.916). Except for C3 and C4, there was significant difference in the measured values of the same side (P < 0.05). (5) The above results suggest that the lower cervical pedicle entry point and the lateral concave between articular process have a relatively constant orientation relationship in transversal section and a large variation in the sagittal section.

Objective To identify the role of protein kinase C(PKC) activation in ischemic myocardium proctection and its effect after inhibition by polymyxin B sulfate.Methods We randomly divided 30 rabbits into ischemic(IS) group,ischemic preconditioning(IPC) group and polymyxin B sulfate(PMB) group.According to the method reported in previous literature,we simultaneously recorded ECG,pressure curve and monophasic action potential(MAP) throughout the ischemia and reperfusion perocess.Results The rabbit heart rates were not changed significantly during the ischemic or reperfusion period.The products of heart rate multiplying systolic pressure were significantly higher in IPC group compared with the other two groups(P

Objective To evaluate the diagnostic value of color Doppler ultrasound to diagnose senile cataract and complication.Method Color Doppler ultrasound were used to check and analyze 185 eyes senile cataract of 120 patients and were compared with slit-lamp.Results In initial phase of senile cataract,the accuracy of ultrasonography was lower than slit-lamp.The false negative ratio of ultrasound was 15.22%(7/46).In middle and last phase of senile cataract,the relevant ratio of ultrasonography Was 100.00%(139/139).Conclusions The diagnosis sensitivity of color Doppler ultrasound in senile cataract is similar with slit-lamp.Ultrasound is the first choice to diagnose senile cataract in middle and last phase,especially when it is not seen by funduscope.Color Doppler ultrasound is an important way to diagnose senile cataract and complication.

Objective To study the effectiveness and safety of combined laparoscopy and gonadotropin-releasing hormone agonist (GnRH-a) in the treatment of endometriosis (EM).Methods From January to December 2010,198 patients with EM undergoing treatment in Department of Obstetrics and Gynecology,Beijing Anzhen Hospital were randomly divided into three groups,which include 52 cases treated by only laparoscopy in laparoscopy group; 76 cases treated by laparoscopy combined with domestic Leuprolide acetate with dose of 3.75 mg every 28 days in Leuprolide acetate group; 70 cases treated by laparoscopy combined with imported Goserelin acetate with dose of 3.6 mg every 28 days in Goserelin acetate group.The efficacy,pregnancy rate and adverse reactions were compared among the three groups.Results Thirteen cases lost following up,including 3 cases in laparoscopy group,6 cases in Leuprolide acetate group and 4 cases in Goserelin acetate group.(1) Effective rates:effective rates were 47 ％ (23/49)in laparoscopy group,77％ (54/70)in Leuprolide acetate group and 74％ (49/66)in Goserelin acetate group.Compared with laparoscopy group,the effective rate of Leuprolide acetate group and Goserelin acetate group was significantly elevated (P ＜ 0.05).There was no statistically significant difference between Leuprolide acetate group and Goserelin acetate group (P ＞ 0.05).(2) Recurrence rate:recurrence rate were 33％ (16/49) in laparoscopy group,13％ (9/70)in Leuprolide acetate group and 12％ (8/66)in Goserelin acetate group.Compared with laparoscopy group,the recurrence rate of Leuprolide acetate group and Goserelin acetate group was significantly declined (P ＜ 0.05).There was no statistically significant difference between Leuprolide acetate group and Goserelin acetate group (P ＞ 0.05).(3) Pregnancy rate:the number of patients require fertility were 28 cases in laparoscopy group,39 cases in Leuprolide acetate group and 35 cases in Goserelin acetate group.After 2 years follow up,pregnancy rate of 62％ (24/39) in Leuprolide acetate group and 60％ (21/35)in Goserelin acetate group were high than 39％ (11/28)in laparoscopy group significantly,which did not reached significant difference (P ＞ 0.05).(4) Adverse drug reaction:rates of a adverse reactions were 21％ (15/70) in Leuprolide acetate group and 20％ (13/66) in Goserelin acetate group,including irregular vaginal bleeding associated with low estrogen level.There was no significant difference in adverse reactions (P ＞ 0.05).Conclusions Compared with laparoscopy alone,laparoscopy combined with GnRH-a is more effective in treatment of,which exhibit lower recurrence rate,higher pregnancy rate and fewer adverse reactions.Domestic Leuprolide acetate have similar safety and efficacy compared with imported GnRH-a.