OBJECTIVE:To probe into the current situation and developing trend of the marketing of Cefuroxime in China.METHODS:To analyze the production and consumption of Cefuroxime in China.RESULTS&CONCLUSION:We con?sider that the market of Cefuroxime looks to further briskness.However,with more and more manufacturers entering into,the competition is becoming more intensive.

Excision repair cross complementing group 1 (ERCC1) gene is an enzyme of the speed limit of DNA repair protein in nucleotide excision repair (NER) pathways.Researches suggest that ERCC1 has been associated with cisplatin resistance in non-small cell lung cancer (NSCLC) patients.Thus,ERCC1 gene is a new target in malignant tumor gene therapy research,providing a new approach for the treatment of malignant,especially NSCLC.

Setting quantity and quality of services as the base, patient satisfaction as the core of the performance appraisal and salary distribution system are important content of the current comprehensive reform of township hospital. To guarantee the profits for public in township health cares, prevent profiteering behavior, mobilize the staff’s work enthusiasm and establish an effective performance appraisal and salary distribution system are the most focused and considered problems for a great number of township health cares. The example hospital introduced the “progressive scoring” and “incremental increase in return”, which had positive effect.

Matrix metallopmteinase 9(MMP-9) is one kind of matrix metalloproteinases and plays a role in the process of breast cancer metastasis. It can not only degrade the basement membrane and the extracellular matrix around tumor tissue but also promote angiogenesis, even create conditions for the growth of tumors, local invasion and distant metastasis. Current study shows that the over-expression of MMP-9 can significantly speed up the breast lymph node metastasis and the relevant research will provide guidance for clinical treatment and prognosis of breast cancer.

Objective: To observe the impact of vascular calcification on kidney injury rats with the expressions of β-Klotho, fibroblast growth factor receptor 1 (FGFR1) in kidney tissue in order to find the predictor for early chronic kidney disease (CKD), to provide the prevention and investigation basis of vascular calcification and CKD. Methods: Vascular calcification model was induced by vitamin D3 and nicotine injection in experimental rats and the animals were divided into 2 groups: Normal control group and Calcification group. n=6 in each group. Serum levels of creatinine and urea nitrogen were examined by sarcosine oxidase method and UV-glutamate dehydrogenase method respectively; blood levels of calcium and phosphorus were detected by biochemistry method; kidney tissue alkaline phosphatases (ALP) activity was measured by ALP detection kit, protein expressions of β-Klotho and FGFR1 were assessed by ELISA.Results: Compared with Normal control group, Calcification group showed increased serum levels of creatinine (35.200±4.087) umol/L vs (26.000±5.0990) umol/L and urea nitrogen (6.900±0.623) mmol/L vs (5.400±0.803) mmol/L, both P<0.05; elevated kidney tissue ALP activity (60.510±31.090) U/g vs (26.590±8.664) U/g and β-Klotho protein expression (9.052±1.238) ng/mg vs (6.860±1.036) ng/mg, both P<0.05. Blood levels of calcium, phosphorus and kidney tissue FGFR1 protein content were similar between 2 groups. Conclusion: Large dose vitamin D3 and nicotine injection may induce vascular calcification and early CKD symptom in experimental rats; β-Klotho protein expression was significantly increased suggesting that β-Klotho had been involved in the early regulation of vascular calcification and it could be used for the early diagnosis of CKD at certain point.

Open ocular trauma in children is one of the main causes of non-congenital monocular blindness in children. Children’s psychological and daily activities are different from those of adults; the eye tissue structure is not maturely enough, and the characteristics of high response to injury and incompatibility with various diagnostic and treatment methods determine the factors that cause injuries and injuries in children with open ocular trauma. The environment, diagnosis and treatment, and prognosis of children are more complicated than adults. For the emergency of first and second stage on diagnosis and treatment of children with open ocular trauma, it should follow the principles of adult open ocular trauma management, combined with the children's characteristics, clarifying the anatomical location of the ocular trauma before surgery, and closing the wound during the first-stage operation. At the same time, the disturbance of intraocular tissue should be minimized, the timing of the second stage operation and the individualization of the operation design, and the medication and care after the operation should also be combined with the characteristics of children. Prevention of ocular trauma in children is the focus of attention. In the new information era, clinical research and public information platforms should be fully utilized to advance the epidemiological study of children's ocular trauma and guide the construction of the prevention and treatment system for children's ocular trauma more scientifically.

Objective To evaluate the outcome of stereotactic aspiration combined with gamma knife radiosurgery in the treatment of cystic brain metastasis.Methods The study included 21 cases of cystic brain metastasis.There were 16 males and 5 females,with their age ranged 45～78 years old(mean,64.5 years old).Nineteen patients had a solitary tumor and 2 patients had two lesions.The mean diameter of tumors was 36 mm.The Karnofsky Performance Score(KPS) was ≥50%.A stereotactic aspiration was performed under CT guidance to drain the cystic fluid.Then the MASEP-SRRS gamma knife radiosurgery was performed.All brain metastasis were averagely enclosed within the 40%～65%(mean,50%) isodose shells of the radiosurgical fields with a peripheral dose of 16～22 Gy(mean,19.5 Gy).Results All the cases were followed for 1～3 months(mean,2.3 months) with CT or MRI scanning.Tumors completely disappeared in 12 lesions,subsided in 9 lesions,changed less than 10% in 1 lesion,and enlarged in 1 lesion.Cerebral necrosis and edema adjacent to lesions respectively developed in 1 case.No other severe complications were seen.The control rate of tumor was 95.7%(22/23).Twenty-one patients were followed for 6～36 months(mean,23.5 months).The followed-up results showed improvement of symptoms in 18 cases,no change in 2 cases,and exacerbation in 1 case.The survival rate at 6 months was 90.5%(19/21).And the 1-and 2-year survival rates were 62.5%(10/16) and 25%(3/12),respectively.One case survived beyond 3 years.The median survival time was 14.1 months.Conclusions Stereotactic aspiration combined with gamma knife radiosurgery is an ideal treatment for cystic brain metastasis.

Objective To detect the correlation between the periodontal bone loss and carotid calcifications by using MSCT.Methods Imaging materials of 270 patients who were suspected atherosclerosis and underwent MSCT were retrospectively studied.According to the score of carotid calcification,the patients were divided into three groups:slight calcification group (carotid calcification score <100),severe calcification group (carotid calcification score ≥100),and normal control group (carotid calcification score=0).The original images were post-processed with volume rendering(VR),multi planar reformation(MPR),curved planar reformation(CPR)and maximum intensity projection (MIP)by using EBW4.5 workstation,and then the residual teeth number and the periodontal bone loss teeth were recorded in different groups.Results On MSCT,the carotid calcification score and the number of periodontal bone loss were displayed clearly.The number of the periodontal bone loss teeth in the calcification groups were significantly higher than those in the normal control group(P<0.01).Furthermore,the number of the periodontal bone loss teeth was positively correlated with the severity of carotid calcification.Conclusion The periodontal bone loss is highly correlated with the severity of the carotid calcification.The volume reconstruction technique of MSCT is a non-invasive diagnostic testing to detect the periodontal desease and to quantify carotid calcifications.

Objective To screen and identify proteins that interact with receptor for advanced glycation end products (RAGE) with T7 select phage display system. Method GST-tagged fusion protein of RAGE was coated on glutathione suporflow resin and binding screens were performed with human lung T7 phage library. Results After 6 rounds of biopanning, 64 independent plaques were scraped and processed with EDTA. The inserted fragments in these plaques were amplified by PCR and the PCR products were purified by a gel recovery method. The sequences of the insertions were identified and analyzed with BLAST program in GeneBank. Twenty-five clones of encoding proteins were found. Conclusions T7 select phage display system is a convenient, rapid and effective method for screening binding proteins. This study provides an approach to explore complicated functions and novel mechanisms of RAGE-related signal transduction pathways.

Objective To construct pSUPER-ARPC2 vector and to obtain its expression in eukaryotic cell, in order to down-regulate the expression of ARPC2. Methods Oligonucleotide sequences specific for ARPC2 were designed for RNAi, and cloned into pSUPER.basic vector after annealing by restriction enzyme digestion and ligation. After identification by restriction enzyme digestion and sequencing, the plasmid of positive clones was transfected into HEK 293 cells for three times. The expression of ARPC2 was detected by RT-PCR and Western blot. Results It was found that the expression of ARPC2 was definitely knocked down by transfection with pSUPER-ARPC2. Conclusions The RNAi expression vector of ARPC2 was constructed successfully, which was showed to possess the ability to knockdown the expression of ARPC2 by different methods. So, pSUPER-ARPC2 should be a powerful tool in the study of function of ARPC2.