Objective: To prepare the processing solution of realgar by bacteria and to explore the change of pharmacodynamic action and toxicity. Methods: The processing solutions of realgar were prepared using the strain thiobacillus ferroxidans, the realgar powder processed by normal method was selected as control. The effects on RPMI8226 cell proliferation of them were investigated by methyl thiazolyl terazolium (MTT),cell cycle distribution was determined by FCM in RPMI8226 cell. The distribution of arsenic concentration in blood and tissues including heart, liver, spleen, lung, and kidney in the samples were determined by Inductively Coupled Plasma-atomic Emission Spectrometry (ICP-AES). Results: Compared with control group, the solubility rate and the induced apoptotic rate increased, and dosage significantly reduced, while arsenic was hardly found in the tissue in processing group by bacteria. Conclusions: The processing group by bacteria may be enhanced medicinal efficacy and inhibited the toxicity in realgar. It’s intending to provide new approaches for scientific processing realgar.

The presence of pre-S2 protein in the kidney was investigated in 30 patients with biopsy-proved glomerulonephritis (GN) using indirect immunoperoxidase technique and monoclonal antibody. HBsAg and HBeAg in the kidney were detected using the same techniques and HBsAg, HBeAg, anti-HBs, anti-HBe and anti-HBc in serum were assayed at the same time.It was shown that pre-S2 prtein was present in the kidney in 7/22 patients with primary GN and 6/8 with lupus nephritis. It was deposited along the glomerular capillary walls or within the mes-angium, depending on the type of GN as well as the deposition of immunoglobulins and complement components in the kidney. Deposition of pre-S2 protein in renal tissue was correlated with HBsAg deposition in the glomeruli, but not HBeAg deposition. In membranous GN, there was a good correlation between the presence of pre-S2 protein in the kidney and virus antigetiemia. It is concluded that pre-S2 protein detection in the kidney is of value in evaluating the hepatitis B virus associated GN.

Objective To explore the clinical effect of electrochemical therapy (EchT) on hemangiomas of liver.Method 186 cases of hemangiomas of liver with,male 97 and female 89 were selected for the study.The age ranged from 31～55 years old.The electrochemical therapy instrument of model ZAY-B and the electrode made of platinum were used.Suitable manipulating technique and treating quantity of electricity were investigated for the patients.Electrodes were inserted into the tumor through a tracer directly by opening the abdominal cavity.Normal tissue of liver was protected by the spaghetti and the electrodes were connected to EchT device,then the treatment began.The therapeutic voltage was 6～8V,electic current 120～160mA.The quantity of electricity was 100～150 Coulombs per 1.0cm diameter of the tumor.The treatment could be terminated when the tumor became from soft to stiff.Result The therapeutic efficacy was evaluated 6 months after EchT.Through the follow-up of 1～5 years,103 of total 186 cases of hemangiomas of liver completely disappeared(72.5%);31 cases partially relieved (21.8%) and 8 cases were ineffective (5.6%).Conclusion EchT to treat hemangiomas of liver is safe and efficacy,with less traumatic and a quick recovery.It can be served as a new method for treating hemangiomas of liver.

0.05). Conclusions Cyclin D1 and p16 are important factors modulating cell cycle. The interrupt of balance between these two factors derived from abnormal expression of cyclin D1 may be one of the causes of vulvar white lesion.

The paper is to report the preparation of realgar bioleaching solution (RBS) by bacteria and the comparison of pharmacokinetics of RBS and H3AsO3 (ATO), and the study of its possible change of absorption and distribution of soluble arsenic in rat. The experiment was carried out on Wistar rats given peritoneal injection of RBS at a dose of 0.3 mg x kg(-1) (soluble arsenic content, 0.3 mg x kg(-1)), and rats given ATO at the dose of 0.3 mg x kg(-1) (soluble arsenic content, 0.3 mg x kg(-1)). The arsenic concentrations in many tissues including heart, liver, spleen, lung, renal and brain were determined. The changes of pharmacokinetic parameters and arsenic distribution in different tissues were detected and compared in these two groups of rats. The pharmacokinetic parameters of RBS and ATO are very similar. There is very few distribution of arsenic in the tissues in RBS group, compared with the ATO group. There is significant difference in the content of arsenic between two groups statistically (P < 0.01). It is feasible that we select the bacteria bioleaching solution as a candidate drug, which may be employed for primary change of arsenic compounds including dissolved inorganic arsenic and organic arsenic, in order to improve bioavailability and decrease the amount of arsenic accumulation in animal tissues. In addition, there is significant difference in the change of arsenic compounds between two groups. It can be concluded that, the investigation on application of microbial technology may provide a basis for exploratory research of realgar.

The quality of teachers is the key to enhance the effectiveness of "Two Courses" teaching.This paper mainly talks about the basic qualities of "Two Courses"teachers,such as the ideology and politics,professional ethics,theory,business technology,capacity and so on.

A clinico-pathological study was carried out on 8 patients with microscopic polyar-teritis nodosa (MPAN). It was found that the renal lesions were very common and severe in this group of patients apart from the typical presentation of MPAN, including fever, myalgias, arteritis and the involvement of hematologic, gastrointestinal and cardiovascular systems. Laboratory pictures were characterized by leucocytosis, anemia and increased serum creatinine. The plasma anti-neutrophil cytoplasmic antibodies were present and thought to be of particular importance. On light microscopy, focal, segmental necrotising glomerulonephritis was discovered which was often associated with extensive crescence formation and vasculitis. We recommend renal biopsy as a valuable tool of investigation in the diagnosis of MPAN.

Objective To validate the clinical performance of a domestic fibrinogen reagent by freezing method(Clauss method) on the coagulometer Japan Sysmex CA1500.Methods The domestic fibrinogen reagent as the reagent A and the Germany Siemens Dade thrombin reagent as the reagent D,the Clauss method was adopted to measure the within-run precision and between-run preci-sion in two levels of quality control respectively.The reference value range was verified by the reagent A in 165 cases of normal clin-ical samples.The fibrinogen detection results in 200 cases of clinical samples were compared between by the reagent A and the rea-gent D.The significance test and the equivalence test were performed.Results The within-run precision CV of the reagent A and D in two levels of quality control were 4.28%,6.98% and 3.45%,5.22% respectively,the between-run precision CV of the reagent A and D in two levels of quality control were 6.23%,10.34% and 6.20%,9.89% respectively,the differences had no statistical significance(P >0.05).The reference value range of the reagent A was 2.08 -3.92 g/L.The fibrinogen detection results of the clinical samples by the reagent A and D had the statistically significant differences (P =0.025).But the 90% bilateral confidence in-terval(90%CI :-0.09,0.15)of the difference in the mean detection results by these two reagents located in the equivalent interval (-0.27,0.27).Conclusion The domestic fibrinogen reagent for Clauss method has reliable detection results and is suitable for the coagulometer Japan Sysmex CA1500,which is equivalent to the clinical application of Germany Siemens Dade thrombin reagent.

Objective To analyze the imaging manifestations of chondromyxoid fibroma,so as to improve diagnostic accuracy.Methods The X-ray,CT and MRI manifestations of 8 cases with chondromyxoid fibroma confirmed by surgical pathology were analyzed retrospectively.Results All of 8 cases were in the long tubular bones.X-ray showed oval,well defined,eccentric,radiolucent lesion with surrounding sclerosis.The thick dense bony or coarse reticular septation were seen in 6 cases.CT scan showed osteolytic,surrounding sclerosis,and 1 case with foci of calcification.MRI images showed a low or medium signal on T1WI,high signal on T2WI,1 case with a equal T1WI and slight high T2WI signal intensity peripheral rim around.Two cases with diffused enhancement on T1WI after intravenous injection of gapentetate acid meglumine injection and a peripheral liner enhancement in 1 case.Conclusion Chondromyxoid fibroma has certain imaging characteristics,but should be combined with X-ray,CT and MRI to differentiate from other bone tumors with similar imaging manifestations.

Objective To optimize the flash type extraction ofDanzhuye Granules.Methods With the total flavonoids and extract yield as indices, the orthogonal test was adopted to optimize the extraction times, the ethanol concentration, solid-liquid ratio, and the duration of extraction forDanzhuye Granules.Results The factors that infuenced the extract efficiency from high to low were the extraction times, volume fraction of ethanol, the ratio of liquid to solid, and the extraction time. The optimum extracting condition included that the origin was extrated by the ethanol volume fraction 80% with the material to liquid ratio 30:1, total of 3 times, with each time of 3 minutes. Validation experiments of the potimal condition showed that the extraction of total flavonoids was 29.1 mg/g, and the extract yield was 23.6%.Conclusions The flash type extraction method is suitable for extraction, which provides a new method for the development and utilization ofDanzhuye Granules.