The compounds were isolated from Aoonitum coreanum (Lev1.) Rapaics and identificd by TLC, IR, MS and NMR, as ?-sitosterol (Ⅰ), daucosterol (Ⅱ), Guan-Fu base A (Ⅲ),GuanFu bas. G (Ⅳ), condelphine (Ⅴ), Guan-Fu base Ⅰ (Ⅵ), hetisinone (Ⅶ), isoatisinum chloride (Ⅷ), atisinum chloride (Ⅸ), and Guan-Fu base z (Ⅹ) from the root. Compounds Ⅲ and Ⅸ were also found in the aerial parts of the plant. compounds Ⅱ, Ⅴ, Ⅶ and Ⅷ were obtained for the first time from this plant. Most of them exhibited antiarrhythmic activlty.

Erythrocytes were frozen with DMSO of different concentrations (not over 20%) as a cryoprotec-tive agent in freezers of different temperatures.The rate of temperature drop was not controlled. Of the erythrocytes actually frozen, the average recovery rate after thawing was 91.3 per cent (range 87.5~ 95%). The average survival rate of erythrocytes stored at -20℃, -30℃, or -80℃ for up to eight months was 90.9 per cent (range 88.2~ 94.5). As compared with glycerol, which is in common use internationally, DMSO seems to be a better cryoprotective agent.

In recent years, we have tried transfusion of large numbers of granulocytes in the patients withsevere infection due to gram-negative bacteria or the patients treated with various types of antibioticswithout effect. The results were quite satisfactory. We know from experience that the curative effectis related to the number of the granulocytes transfused. Only by increasing it to 6-8?109 or 6-8?1016 the curative effect can be achieved.

Objective To understand the status of knowledge,attitude and practice(KAP)of foodborne parasitic diseases among senior high school students in Three Gorges Reservoir Region. Methods A total of 1 353 senior high school students were selected with the cluster sampling method and investigated with the questionnaires in Zigui County,and the data were ana?lyzed statistically. Results Among the 1 353 students surveyed,the awareness rates of parasitic diseases,their hazards and transmissions were 62.23%(842 cases),80.78%(1 093 cases)and 83.89%(1 135 cases)respectively. The awareness rate of parasitic diseases in the students of senior Grade One was higher than that of Grade Two(χ2 =7.037,P<0.05). The formation rates of behaviors of refusing of raw food,refusing of unboiled water,and dining at home or school in the students of senior Grade One were higher than those of Grade Two(χ2=6.970,12.749 and 12.921 respectively,and all P<0.05). Fifty?one per?cent(690 cases)of students would not have the food with the infection risk of parasitic diseases. Conclusion The awareness of parasitic diseases of high school students in Zigui County is not efficient,and the health education should be strengthened.

0.01).After incubation with trehalose for 4h,the expression of CD62p increased,and it decreased when platelets activation inhibitor PGE-1 and adenosine were added to the incubation buffer.The expression of PAC-1 showed no difference among the three groups. Conclusion The preprocessing before platelets lyophilization has no significant effects on the function and activity of platelets when the platelets activation inhibitor is added.

Objective To establish a simple and feasible method of preparing young erythrocytes and test some indexes of evaluating the mean cell age.Methods We collected 34 bags of whole blood from healthy blood donors,400ml/each bag,then eliminated WBCs by leukoreduction filter.After twice centrifuging and manual speration,we finally obtained young erythrocytes from the upper section of the red cell layer,the remains were old erythrocytes.These different age erythrocytes were separated by blood bags.Some indexes as pyruvate kinase,reticulocyte count,mean corpuscular volume(MCV),mean corpuscular hemoglobin concentraion(MCHC),erythrocytes deformation index EI were tested on the non-separation erythrocytes,young erythrocytes and old erythrocytes,simultaneouosly,the detecting hemoglobin recovery rate.Results There was significant difference of all indexes among the three kinds of erythrocyte products.The PK activity ratio of young erythrocytes' to non-preparating erythrocytes was 1 175?0 13,showed the mean cell age was about 40 days.The hemoglobin recovery rate was(48 37?3 82)%.Conclusions The method is effective for preparing young erythrocytes,PK,REI,MCV,MCHC and EI can be used to evaluate the young erythrocytes' mean cell age.

Objective To study the effect of oxigenated leukodepletion filter(OLF), in order to evaluate its potential value in battlefield trauma application.Methods 12 bags of whole blood were filtered by OLFs, while the other 12 by normal filters,some indexes including ,partial pressure of oxygen(PO 2),O 2 saturation of oxygen(SO 2),HbO 2 WBC,RBC counter and volume of RBC supernatant were measured before and after leukocyte filtration.Results PO 2,SO 2 and HbO 2 increased 57%,51% and 53% respectively after filtration by OLFs, the residual leukocyte count decreased to (3 37?0 54)?10 7 each bag,and the leukocyte clearance rate was (97 52?0 74)%.Conclusions OLF can improve the effect of blood transfusion and reduce some acute transfusion adverse reactions.Thereby,application of the OLF has binefits significantly to heal the wounded patients in battlefield trauma.

Object To search for more bioactive alkaloids from Aconitum coreanurn through this fur-ther study. Methods The ethanolic extract from the root of A. coreanum was isolated by aluminium oxidecolumn chromatography, the constituent structures were elucidated mainly by their physicochemical prop-erties and spectroscopic (IR, ESI-MS,1 H-NMR, 13 C-NMR,1 H-1 HCOSY, HMQC, and HMBC)analysis.Results Seven alkaloids were obtained from the root of A. coreanum, they were identified as: 11, 13-di-acetyl-14-hydroxy-2-isobutyryl hetisine ( Ⅰ ), guan-fu base G ( Ⅱ ), guan-fu base F ( Ⅲ ), guan-fu base Z( Ⅳ ), guan-fu base I ( Ⅴ ), guan-fu base K ( Ⅵ ), and guan-fu aminealcohol (Ⅶ). Conclusion Compound I is a new natural product, named as guan-fu base P, its 1 H-NMR and 13 C-NMR data are reported first inthis paper.

In this study, cryopreservated platelets were transfused to 1560 patients. of them, 536 were patients with acute leukaemia, 285 with anemia, 253 with thrombocytopenia due to chemical or radicalized treat, 438 in perioperation, and 48 with DIC or hemorrhage of rupture esophagus varix. The storage time of cryopreserved platelets ranged from one week to thirteen months. Most of the cryopreserved platelets had not been washed before application and no obvious adverse-effects were observed except for garlic smell in patients' exhalation after transfusing cryopreserved platelets. The results showed that transfusion of cryopreserved platelets has a remarkable on effect hemostasis and can enhance the platelet count in peripheral blood. Compared with liquid-preserved platelets, cryopreserved platelets for clinic use have the following advantages:1) the potentiality of large stock, 2) much longer shelf life, 3) higher safety, 4) superior hemostasis effect, and 5) capacity to meet emergent massive clinic demand.

The patients received massive blood transfusion in General Hospital of the Chinese Pepole's Liberation Army between 1999to 2001were reviewed. The following items of leucodepleted blood transfusion (LDBT)group and routing blood transfusion(RBT) group at pretransfusion, the first day, third day, and seventh day after blood transfusion were recorded and compared: glutamic pyruvic transaminase(GPT), glutamic oxaloacetic transaminase(GOT), lactate dehydrogenase(LDH), total bilirubin(TB), direct bilirubin(DB), alkalescence phosphorylase(ALP), total protein(TP), albumin(ALB), glucose (GLU), Ca 2+ , total CO 2, pH and febrile nonhemolytic transfusion reactions(FNHTR). The results showed that compared with RBT group, GPT in LDBT group on the third day after blood transfusion was significantly lower ( P 0 05). When antiallergic medicine was given, FNHTR rate of massive blood transfusion in RBT and LDBT groups was 27% and 1 9%( P