Objective Effects of fucoidan from Cladosiphon okamuranusa (CF)on mice bearing Siso were investigated. Methods Different concentrations of CF were given to mice bearing tumor by ig. The tumor weight, spleen index, thymus index and serum MDA content, serum SOD activity were detected by physical and biochemical methods. Results The growth of S180 tumor was inhibited significantly by CF in different concentrations. The inhibitory rate ranged from 27. 83% to 33. 97%. The highest inhibition rate occurred in the medium dosage. The spleen index, thymus index and leukocyte counts were not influenced obviously. The content of serum MDA was decreased remarkably and the activity of serum SOD was enhanced notably. Conclusion The mechanism of anti -tumor effects of CF may relate with its antioxidation.

Objective:To investigate the impacts of two anesthesia approaches on infections of immunological parameters during splenectomy in hepatocellular carcinoma patients .Methods: Sixty hepatocellular carcinoma patients were divided into two groups randomly,each groups was thirty (liver function Child-Pugh grade was A-B).Total intravenous anesthesia with pmpofol group (group A,n=30 ) and combined intravenous inhalational anesthesia with sevoflurane group (group B,n=30 ).Before induction of anesthesia , at the end of operation ,and after operation 24 hours.blood samples were collected to determined with the level of CD 3+,CD4+,CD8+, CD4+/CD8+and TNF-α, IL-2, IL-6 of hepatocellular carcinoma patients .Results: The perioperative physiological index MAP , HR, SpO2,RR each point had no obvious difference between two groups (P>0.05).The levels of CD3+,CD4+,CD8+,CD4+/CD8+had no significant difference between two groups before anesthesia (P>0.05).There was no significant changes in CD3+,CD8+with two groups in all moments.Compared with the T0,A,B two groups of CD4+,CD4+/CD8+were lower (P<0.05)in T1 point,but T2 point and T0 point had no significant difference in group B .The levels of CD4+,CD4+/CD8+in group B were higher than in group A ( P<0.05 ) . Compared with T0 moment,group A and group B patients postoperative IL-6 in T1,T2 level increased significantly (P<0.05),and there was no statistically significant difference between group A and group B in all moment .( P>0.05 ) .Compared with the T0 moment,there was no significant change in group A of TNF-αlevel (P>0.05),while group B increased significantly in postoperative day ( P<0.05 ) .The level of IL-2 in each moment interval between the two groups had no significant difference ( P>0.05 ) . Conclusion: Both total intravenous anesthesia with pmpofol and combined intravenous inhalational anesthesia with sevoflurane inhibit the immune function of the patients with hepatocellular carcinoma cell immune reaction .The inhibitory effect of sevoflurane inhalation anesthesia on cell immune function is less affected .

Objective:To investigate the effect of dietary cholesterol on hepatic TG accumulation in rats. Method:Fourteen male Wistar rats were randomly divided into 2 groups and fed 1% cholesterol or cholesterol free AIN76 diets. After 4 w,serum triglyceride(TG) ,total cholesterol(TC) ,high density lipoprotein cholesterol(HDL-C) ,phospholipids(PL) ,glucose and free fatty acid(NEFA) levels were determined. Hepatic lipid concentrations(TG,TC,PL) and the activities and/or mRNA expression of malic enzyme(ME) ,glucose-6-phosphate dehydrogenase(G6PDH) ,fatty acid synthase(FAS) ,phosphatidate phophatase(PAP) ,carnitine palmitoyl transferase(CPT1,2) ,HMG-CoA reductase,acylCoA-cholesterol acyltransferase(ACAT) ,cholesterol 7?-hydroxylase(CYP7A) were also determined. Results:The serum TC and non-HDL-C levels were significantly increased but TG and HDL-C levels were significantly decreased by cholesterol feeding. The concentrations of hepatic TC and TG were 4-20 folds higher in cholesterol group than those in cholesterol free group. The activities of hepatic ME,G6PDH,FAS,PAP and CPT were depressed by cholesterol(40%,70%,50%,15% and 25% respectively) . The mRNA expression of FAS,CPT1,CPT2,and HMG-CoA reductase were down-regulated(35%,30%,50% and 25% respectively) and CYP7? and ACAT were up regulated(6.5 and 1.6 fold) by cholesterol in liver. Conclusion:The dietary cholesterol increases TG accumulation in liver,but dose not stimulates the activity and the gene expressionof hepatic TG synthesis related enzymes.

BACKGROUND:Regional myocardial diastolic dysfunction has been recognized as a sensitive and characteristic sign of myocardial ischemia and infarction,train rate imaging(SRI)is a novel ultrasonic technique with good spatial and high temporal resolution,nd it can be used to quantitatively analyze regional myocardial function.OBJECTIVE:To investigate the characteristics of the longitudinal diastolic strain rate(SR)in ischemic and infarct myocardium by SRI,n order to explore the clinical value of SRI in evaluating regional left ventricular diastolic dysfunction in patients with coronary artery disease.DESIGN:A case-control study.SETTING:Department of Cardiology.Second Affiliated Hospital of Sun Yat-Sen University.PARTICIPANTS:All the participants were selected from outpatients and/or inpatients of the Second Affiliated Hospital of Sun Yat-sen University between October 2005 and April 2006.Twenty-one patients with myocardial infarction(MI group)and 11 patients with angina pectoris(AP group)were enrolled in the study.Twenty age-matched healthy subjects served as controls.METHODS:After performing conventional echocardiographic examination,olor tissue Doppler images from apical four-,three- and two-chamber views (posterior septum and lateral wall,nterior septum and posterior wall,nterior and inferior wall)were recorded on magneto-optical disk.Then SR curves were derived from the stored images off-line using QLAB 4.2software.MAIN OUTCOME MEASURES:Ischemic segments in the AP group,nfarct segments in the MI group and normal segments in the control group were analyzed.The parameters measured included peak early diastolic SR (SRe)and peak late diastolic SR(SRa).RESULTS:Compared with the normal myocardium,oth SRe and SRa decreased significantly in the infarct and ischemic myocardium(all P＜0.05).No significant differences were observed with respect to SRe and SRa between the infarct and ischemic myocardium(both P＞0.05).CONCLUSION:SRI can be used to quantitatively analyze regional myocardial diastolic dysfunction in patients with coronary artery disease,ut its value is limited in differentiating infarct from ischemic myocardium.

The immunologic function of extract from Venerupis variegata(EVV)has been studied. The results show:given orally different dose of EVV[50,500,2500 mg/(kg. d)],the spleen weight of mice (P

Objective To compare the acute complication and short term effect between angioplasty and stent in coronary arteries less than 3 mm Methods There were forty two patients and fifty two vessels attending to the study During procedure, native vessel diameter, stenosis degree and MLD were measured pre operation and post operation, and the clinic features of acute occlusion, subacute thrombosis, acute MI, emergent CABG and death were followed within thirty days after operation Results Stent group showed larger balloon, higher pressure during balloon dilatation, larger vessel lumen and lower residue stenosis than balloon group There were no acute MI, emergent CABG and death in two groups, but one subacute thrombosis in stent group and one acute occlusion in balloon group Conclusion Selective stent in small vessel may reduce acute complication and restenosis after interventional therapy

Objective Antiaging effects of seal kidney on aged male rats have been studies. Methods: Reproduction system of male rat was damaged by CYP. After ig. seal kidney 30 days, the content of serum T, serum MDA and the activity of SOD were determined. The changes of histology of the testis were observed by microscope with the method of paraffin embedding and H-E staining. Results The contents of serum T not only in normal rat but also in reproduction system damaged rat were markedly enhanced. The function condition of leydig's cells in the testis was improved. The formation and development of spermatozoon were increased. The activity of serum SOD was remarkably raised , while the content of serum MDA was strikingly reduced. Conclusion Seal kidney has the notable antiaging effects.

0.05).Conclusions Certain cases of GERD may have the symptoms of pharyngolaryngitis.After being treated with omeprazole or tegaserod,the patients with abnormal acid reflux may get significant symptomatic improvements.

Objective To compare the therapeutic effect of aerobic exercise training of different intensity on hypertension. Methods Sixty hypertensive patients were randomly allocated to 2 groups and treated with aerobic exercise training at 20% or 60% of maximal intensity. Not only were the relevanl parameters of exercise capacity measured by the use of treadmill exercise test,but also was ambulatory blood pressure monitored and the quality of life recorded before and after the therapy. Results There was nc significant difference of the decrement in any parameter of ambulatory blood pressure between the two groups ( P>0. 05).There was a significant difference of the increment of general health, vitality, emotion and mentality between the two groups after treatment with group A better than group B(P <0. 05). Conclusions Aerobic exercise training at 20% and 60% of the maximal intensity has similar ambulatory blood pressure, but the quality of life is significantly better after aerobic exercise training at 20% of the maximal intensity.

BACKGROUND:It has been proved that bone marrow mononuclear cel transplantation can obviously improve neurological function of rats with cerebral hemorrhage. OBJECTIVE:To investigate the effects of transplanted bone marrow mononuclear cel s on the neurological function and apoptosis in perihematomal brain tissues fol owing cerebral hemorrhage in a rat model. METHODS:Twenty-four Sprague-Dawley rats were given stereotaxical injection of col agenase IV into the caudate nucleus to establish cerebral hemorrhage models in transplantation group (n=12) and model group (n=12), and then at 6 hours after cerebral hemorrhage, rats in these two groups were administrated 3x1010/L al ograft bone marrow mononuclear cel s and the same amount of PBS, respectively. Another 12 rats were given no interventions as control group. Neurological functions of rats were assessed at 1, 4, 8, 16 days after cerebral hemorrhage;pathological changes of the injury sites were observed at 16 days after transplantation;neuronal apoptosis rates in the perihematomal brain tissue were detected by flow cytometry at 2 and 4 days after transplantation. RESULTS AND CONCLUSION:The modified neurologic severity scores in the transplantation group were significantly lower than those in the model group at 8 and 16 days after cerebral hemorrhage (P<0.05). In the control group, cel s in each layer arranged closely with complete structure, and neurons and glial cel s were in good shape;in the model group, perihematomal brain tissues were loose with intercel ular gap, in which most neurons and glial cel s became necrotic;in the transplantation group, cel s in each layer arranged closely and regularly, and glial cel proliferation occurred. Besides, compared with the model group, the neuronal apoptosis rate in the transplantation group was significantly lower (P<0.05). To conclude, bone marrow mononuclear cel s can significantly enhance the neurological function recovery and reduce neuronal apoptosis in the brain of cerebral hemorrhage rats.