1.Effects of noise-proof earplugs on reduction of noise in a ward with a few patients after gynecological surgery
Modern Clinical Nursing 2014;(11):24-26
Objective To explore the effect of earplugs on the reduction of noises in the wards with a few patients after gynecological surgery.Methods Two hundred and fifty-four patients hospitalized in our hospital after gynecological surgery from March 2012 to May 2013 were divided into control group(n=124)and observation group(n=130). The observation group was managed with earplugs and the control group patients received routine nursing. The two groups were compared in terms of breathing,heart rate,blood pressure,24 h scores on anxiety before and after operation.Results The postoperative respiration,heart rate,blood pressure of the observation group patients was significantly lower than those of control group(all P<0.05),anxiety score was significantly lower than that of the control group(P<0.01). Conclusions Earplugs can create a quiet environment in wards with a few patients after gynecological surgery and help to stabilize vital signs and reduce anxiety.
2.Intracranial vascular stenosis and risk factors in different types of ischemic stroke
Guorong LIU ; Yuechun LI ; Jingfen ZHANG
Chinese Journal of Neurology 2000;0(05):-
Objective To investigate the intracranial vascular stenosis and risk factors in different types of ischemic stroke. Methods 525 patients with ischemic stroke were assessed by a transcranial Doppler. Lipids including total cholesterol (CHO),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) were examined. Results Intracranial vascular stenosis in diabetic and non-diabetic groups of patients,hypertension and non-hypertension groups of patients showed significantly difference (P
3.The Design Method of Introduction in Medical Organic Chemistry Course
Xu WANG ; Jingfen LI ; Weiguo SHI
Chinese Journal of Medical Education Research 2003;0(02):-
In medical organic chemistry education, seven design method of introduction are put forward by using teaching art in educational segment, allowing students to enhance the study activity in lively atmosphere.
4.Aortic arch atheroma and stroke
Yuechun LI ; Jingfen ZHANG ; Ruiping WANG
International Journal of Cerebrovascular Diseases 2009;17(1):41-44
Aortic arch atheroma is one of the causes of embolic stroke. With the deepening of research on aortic arch atheroma, its relationship with stroke is more and more clear, particularly the aortic arch plaque thickness ≥4 mm, is associated with cryptogenic stroke, stroke recurrence, and other vascular events. Although aortic arch atheroma and clinical features of stroke have been further understood, there are still many doubts.
5.Management of complications during percutaneous implementation of biliary stents
Yongchun ZHANG ; Zhanjun GU ; Xiaotao DENG ; Jingfen HAN ; Guozhen LI
Chinese Journal of Radiology 2000;0(12):-
Objective To retrospectively analyze the complications and management of complications during percutaneous implementation of biliary stents in 17 patients. Methods Percutaneous biliary drainage and implementation of biliary stents for malignant biliary obstruction under fluoroscopic guidance was performed in 64 patients. 17 cases developed complications. Bradycardia and hypotension was seen in 5 cases, slipping of the stent into the duodenum in 1, bile duct perforation in 2, the fine guidewire broken in the hepatic parenchyma in 2, malposition of the stent in 3, hepato renal failure leading to hemorrhage in 2, exchanging guidewire broken in the duodenum in 1, and balloon broken leading to stent dislocation in 1, respectively. Results Bradycardia and hypotension in the 5 cases was treated medically and the procedure was interrupted. Slipping of the stent into the duodenum was passed off spontaneously. Broken of the fine guidewire in the liver did not present any complaints until the death of the patients. Perforation of bile duct was treated conservatively with a clinical successful outcome. Malposition of the stent was treated by reimplementation of shorter stents. The broken exchanging guidewire in the duodenum during implementation was removed with the gastroscope. The hepato renal failure leading to hemorrhage had poor prognosis. The broken balloon caused stent dislocation was treated by reimplementation of stent. Conclusion Percutaneous implementation of stent is effective and safe for elderly patients with proximal stenosis of the biliary tract. However, one must be very careful to avoid the complications.
6.Influence of producing area and plant age on oxymatrine content in root of Sophora flavescens
Hongmin LI ; Renquan HUANG ; Jianguo HAO ; Jingfen JIA
Chinese Traditional and Herbal Drugs 1994;0(04):-
Object The influence of producing area and plant age on oxymatrine levels in the dried root of Sophora flavescens Ait. has been investigated to provide useful information to optimize the areas for mass scale propagation, proper management and species conservation. Methods The oxymatrine was quantified by HPLC and various statistical treatments were carried out following SPSS 9.0 and Microsoft Excel. Results The oxymatrine concentrations in the root samples collected from 17 provinces and regions differed from 0.494% to 4.127% and the maximum oxymatrine content (3.493%) was recorded in the root sample collected from Heilongjiang Province. Analysis of variance indicates a highly significant difference in the oxymatrine content of roots collected from different provinces, and the samples from the cold arid northern high-latitude areas had higher oxymatrine than those from warm humid southern low-latitude areas in the mainland China. Plant age is positively related to the total root biomass and oxymatrine content. Conclusion The oxymatrine content in the roots of S. flavescens is quite different between different areas and plant ages. The S. flavescens growing in the cold arid northern high-latitude areas had higher oxymatrine concentration.
7.Monitoring of chimerism and fusion gerne by STR-PCR and RT-PCR in relapse chronic myeloid leukemia patients after hematopoietie stem cell transplantation
Jingfen SUN ; Xiaoping HAN ; Hongshi JIN ; Chunji GAO ; Li YU
Journal of Leukemia & Lymphoma 2009;18(7):392-395
Objective To investigate the value of the multiple short tandem repeat (STR)amplification by fluorescence labeling polymerase chain reaction (PCR) combined with fusion gene bcr-abl mRNA expression for quantitative determination of chimerism and qualitative detection of bcr-abl transcripts,and to evaluate the status of engraftment and predict the outcome of allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods 5 relapse patients with CML after alIo-HSCT were dynamically investigated. Quantitative analysis of donor chimerism was performed by multiplex PCR amplification of STR markers and capillary electrophoresis with fluorescence detection, qualitative detection of bcr-abl transcripts was performed by RT-PCR. Results The donors alleles appeared in all of 5 patients on day 28 post transplant, and bcr-abl expression was negative. But 5 patients had unstable mixed ehimerism. (DC: 0~80.4 %) at the different time points after aIIo-HSCT and bcr-abl was positive. One of them kept eontinuely the mixed chimerism in the relapse of disease, and died after one year, and the other changed from MC to CC by intervention of clinical treatment. Reduction of donor chimerism were detected prior to the occurrence of graft rejection and disease relapse, while bcr-abl gene expression was positive. Conclusion The results of STR-PCR in the range of its sensitivity fully correspond with bcr-abl tests in patients with CML. The combination of STR-PCR with RT-PCR provides a highly sensitive and valuable tool for engraftment evaluation, graft rejection, relapse and predicting GVHD. Furthermore it can provide a basis for early intervention of clinical treatment, and can identify these patients at high risk with molecular or cytogenetic relapse after allo-HSCT.
8.Validation of the silencing site of rhesus monkey P21 gene in COS-7 cells
Yuhan LI ; Jingfen SU ; Chen ZHANG ; Liang SHI ; Yunbo LIU
Acta Laboratorium Animalis Scientia Sinica 2015;(3):297-300
Objective To screen the effective silencing targets of P21 gene at the cellular level in rhesus monkey . Methods To detect the expression of P21 gene in COS-7 cells ( derived from the kidney of African green monkey , Cerco-pithecus aethiops).Four small hairpin RNA (shRNA) sequences targeting rhesus monkey P21 gene were designed and in-serted into lentivirus-based gene silencing constructs FUGW-TDT.The vectors were transfected into COS-7 cells respective-ly.The suppression of P21 mRNA was detected by real-time PCR, and the expression of P21 protein was detected by West-ern blot assay .Results Four gene-silencing sequences were screened that lied in 541-561 bp, 542-562 bp, 215-239 bp, and 624-648 bp of the rhesus monkey P21 mRNA.Their silencing rate was (91.82 ±3.21)%, (82.47 ±2.48)%, (81.31 ±2.69 )% and ( 87.35 ±4.59 )%, and the protein expression was ( 11.97 ±0.70 )%, ( 20.22 ±0.65 )%, ( 23.21 ± 0.63)%and (14.42 ±0.86)%, respectively.Conclusions Four effective silencing target sequences are screened at cel-lular level , which can be used in gene silencing research of rhesus monkeys .
9.Matrix Metalloproteinases and Atherosclerotic Plaques
Jinfeng ZHANG ; Guorong LIU ; Jingfen ZHANG ; Ruiming LI
International Journal of Cerebrovascular Diseases 2008;16(2):141-144
Matrix metalloproteinases (MMPs) degrade extracellular matrix,participate in the atherosclerosis (As) formation and plaque rupture.They are associated with the stability of plaque.Tissue inhibitor of metalloproteinase (TIMP) is a specific natural MMP inhibitor.The disequilibrium between MMPs and TIMP is closely associated with the formation and development of AS.Therefore,it is a new approach for the prevention and treatment of cardiocerebrovascular diseases by regulating the equilibration between MMPs and TIMP, delaying the development of AS and preventing plaque rupture.
10.Detection of Candida albicans fructose bisphosphate aldolase antigen-specific T cells in healthy individuals by ELISPOT assay
Yuan HU ; Jingyu WANG ; Xiujuan SHANG ; Wei LI ; Yan ZHOU ; Jingfen LU ; Fangqiu LI ; Lining SHI
Journal of Medical Postgraduates 2017;30(6):565-568
Objective Invasive candidiasis is associated with a significant mortality clinically.The purpose of this study was to observe the T cells immune response to fructose bisphosphate aldolase (Fba), an immunodominant antigen of Candida albicans, and determine whether the antigen has the possibility of priming cellular immune protection in invasive candiasis.Methods Using ELISPOT assay, we determined the frequencies of positive spot-forming cells (SFCs) of Fba antigen-specific T cells secreting IFN-γ, IL-4 and IL-17A in the peripheral blood mononuclear cells (PBMCs) of 26 healthy individuals.Results After Fba stimulation, the frequencies of positive SFCs of IFN-γ, IL-4 and IL-17A in the 26 healthy subjects were 23 (9.75, 42.50), 0 (0, 0.25) and 1.5 (0.75, 8.25), respectively, with statistically significant differences among the three (P<0.01).The response rates of IFN-γ (100% [26/26]) and IL-17A (76.92% [20/26]) were significantly higher than that of IL-4 (15.38% [4/26]) (P<0.01).Fba-induced strong response (SCFs ≥20) for IFN-γ was observed in 57.69% (15/26) of the healthy individuals, that for IL-17A in only 1, while that for IL-4 in none.Responses of both Th1 and Th17 cells to Fba were found in 65.38% (17/26) of the subjects, that of Th1 cells in 19.23% (5/26), but that of Th2 cells in none.Conclusion Fba of Candida albicans can induce immunodominant responses of Th1 and Th17 cells and is a potential vaccine against invasive candiasis.