OBJECTIVE:To establish a method for the determination of residual methanol in sucralose. METHODS:Head-space capillary GC method was adopted. The determination was performed on DB-624 capillary column by temperature program-ming(initial temperature of 35 ℃,maintaining for 5 min,then raising to 200 ℃ at a rate of 50 ℃/min,maintaining for 5 min), the detector was FID. The temperature of the injector was maintaining at 220 ℃ and that of detector at 250 ℃,carrier gas was ni-trogen gas at the flow rate of 2.0 ml/min,the split ratio was 10∶1,headspace temperature was 80 ℃ and equilibrium time was 30 min,and the volume was 1 ml. RESULTS:The linear range of methanol was 98.99-296.96 μg/ml(r=0.999 9);RSDs of preci-sion,stability and reproducibility tests were all no more than 2.16%;the average recoveries were 100.20%(RSD=0.48%,n=9). The limit of detection(LOD)of sucralose was 1.39 μg/ml. In samples from 3 manufacturers,the contents of residual methanol in one manufacturer were 3.12 μg/ml,and the other two were lower than LOD. CONCLUSIONS:This method is simple and fast, and suitable for the determination of residual methanol in sucralose.

Objective To explore the possibility of differentiating functional islet-like masses from bone marrow stromal cells induced by DMSO. Methods Adult Wistar rat bone marrow stromal cells(MSC) were isolated and cultured then induced for 3 days with serum free H-DMEM media supplemented with 1% DMSO.The cells were then cultured in H-DMEM supplemented with 10% FBS for 7-10 additional days.Morphological changes of the induced cells were observed under an inveted microscope.DTZ staining,immunocytochemical stainings were conducted to examine the production of insulin and glucagons.Glucose challenge and radio immunoassay were perfomed to evaluate the function of the aggregates,and RT-PCR was conducted to detect the expression of endocrine gene. Results While MSC cells were typically fibrocyte-like,grape-like coherent clustered cells were observed in experiment group on the 3rd day after the inducement of DMSO,which were similar to pancreatic islets.DTZ staining of the cell aggregates was positive as the natural pancreatic islet.Immunocytochemistry also confirmed that these aggregates were positive for nestin on the first day after the inducement of DMSO,and positive for insulin,glucagen on the 10th day after the inducement;while the control group were negatively stained for the characteristics of endocrine.RT-PCR showed the insulin1,insulin2,glucagon and somatostaitin gene expression on the 10th day after the inducement of DMSO.Conclusion Islet-like masses can be transdifferentiated from bone marrow cells by the inducement of DMSO.

Objective To investigate the serum levels of Thyroid stimulating hormone (TSH ) ,Thyroglobulin antibody (TgAb) and Peroxidase antibodies(TpoAb) in patients with different thyroid diseases and to explore the relationship between the 3 indexes with thyroid diseases .Methods 383 cases of thyroid diseases were divided into six groups ,simple adenoma of throid group(TA , n=30) ,nodular goiter group(NG ,n=59) ,papillary thyroid hyperplasia group(PTH ,n=36) ,nodular goiter with adenoma group (NG+ TA ,n=196) ,hashimoto′s thyroiditis(HT ,n=13) ,throid carcinoma group(TC ,n=49) .The relationship between concentra-tion of FT3 ,FT4 ,TSH ,TgAb ,TpoAb in serum and thyroid diseases incidence was analyzed .Results (1)The serum TSH concen-tration had statistically sinificance between group HT with group TA and NG ,group PTH and TA+NG group(P<0 .05) .Despite the concentration of serum TSH in TC group was in a high level near to the upper limit ,there was no statistically significance ver-sus other groups .(2)In group HT and TC ,the increased rate of TSH and positive rate of TgAb and TpoAb were significantly high-er than in group BT (P<0 .05) .(3)The double positive rate of TgAb and TpoAb ,the single positive rate of TpoAb in group HT and TC were higher than those in group BT (P<0 .05) .Conclusion There was a close correlation between serum TSH ,TgAb , TpoAb concentration and thyroid disease ,especially closely associated with HT and TC .Raised TSH and TpoAb positive have im-portant reference value in diagnosis of TC .

Objective: To investigate the possible pathogenesis and report the postoperative results of the late tricuspid regurgitation (TR) after left cardiac valve replacement. Methods: 56 patients developed severe TR after left cardiac valve replacement, including 10 patients with normal prosthesis valve function (group A) and 46 patients with prosthesis valve dysfunction (group B). Four patients underwent mitral valve replacement (MVR) and 6 patients underwent mitral and aortic valve replacement (DVR) in group A. In group B, 36 patients received MVR, 4 aortic valve replacement (AVR) and 6 DVR. Ten patients underwent tricuspid De Vega annuloplasty and 46 patients' tricuspid valves were normal during the initial operation. The surgical treatment of tricuspid valve included tricuspid valve replacement (TVR) in 9 and tricuspid valve plasty (TVP) in 47 at the second operation. Results: Two patients died postoperatively with hospital mortality of 3.6%. The 54 survivors were followed up from 6 to 132 months, mean 79.4 months. The heart function improved significantly in 8 after TVR and 40 after TVP. However, echocardiography showed moderate TR in 5 and severe TR in 1 patient after TVP and medical treatment was needed. Conclusion: The sustained pulmonary hypertension, irreversible right heart impairment, resumption of left ventricular function and sustained atrial fibrillation may be responsible for the development of late TR after left cardiac valve replacement. TVR may achieve a reliable result for severe functional TR and rheumatic tricuspid valve lesion. In some patients with TVP during the follow up, the TR might become more serious.

OBJECTIVE To survey the varieties of microorganism on nurse hands and probe into its controlling measure in general hospital.METHODS We sampled microorganism of nurse hands during intravenous fluids infusion in the Respiratory Department,the Cardiovascular Department,the Department of Digestion,Gynecology and Obstetrics Department,the Department of Thoracic Surgery and the Department of Organ Transplantation in our hospital.All samples carried out co-cultures of fungi and bacteria.RESULTS The varieties of microorganism included not only opportunistic pathogens such as Acinetobacter,Corynebacterium,Pantoea agglomerans(Enterobacter agglomerans) former,and Escherichia coli,but also strong pathogenic microorganisms such as Staphylococcus aureus,and it is so common that finding meticillin-resistant coagulase-negative staphylococci.CONCLUSIONS Nursing staff's hands contamination is serious in the process of manipulating operation in general hospital,emphasis should be placed on prophylaxis and control for nosocomial infection.

Objective To establish a method of isolating and purifying islets from Wistar rat, and to evaluate the biological characters of the isolated and purified islets. Methods The pancreases were surgically removed from adult Wistar rats, carefully minced with scissors as small as possible prior to incubation in 0.9 mg/ml collagenase (Sigma, type Ⅴ) for 10~17min at 37℃. The digested tissue was filtered through a 60 apertures/cm~2 metal sieve and washed with 10% fetal bovine sera (FBS) in Hank’s solution. Purification was performed using Dextran discontinuous density gradient centrifugation to separate the contents of the postcollagenase pellets. Islet samples were stained with DTZ staining for identification. The viability of the purified islet was determined by acridine orange/propidium iodide (AO/PI) double staining. The in vitro function was assayed by different densities of glucose incubating islets for 45min, respectively. Insulin secretion in the supernatant was assayed by radioimmunoassay. Islets were transplanted under the kidney capsule into recipient SD rats that had been rendered diabetic by injecting streptozotocin 50mg/kg, so as to evaluate the in vivo function of islets. Results The islets were recovered between the 11% and 22% Dextran layers, and between the 11% and 1640 layers. The average number of isolated islets was 2181?14 per rat pancreas. After a discontinuous Dextran density gradient purification, about 1826?24 islets were obtained from per rat pancreas, with an average purity over 95%. Islets showed good response to glucose and high efficiency to invert hyperglycemia 7-10d after transplantation. Conclusion These results showed that the digestion methods and purification procedure which we have developed could provide islets from rats for transplantation studies. The biological characters of islets harvested by our method were very well.

Objective Studies on the changes of gastrin and SS immunoreactive cells in pancreatic islets during experimental gastric ulcer. Methods The immunohistochemical ABC technique was used. Results The gastrin immunoreactive cells were located in most of the pancreatic islet. The mumber of G cells in experimental gastric ulcer group were higher than that of control group on the 4th and 10th day after operation.The D cells raised on the 10th day.Conclusion The present work provides the evidence that the G and D cells of pancreatic islets might be involed in the self-healing process of the experimental gastric ulcer by endocrine or paracrine regulation.

Objective Studies on the changes of ? EP, 5-HT, 5-HTR immunoreactive cells in pancreatic islets during experimental gastric ulcer. Methods The immunohistochemical technique was used. Results The numerical densities on area of ? EP, 5-HT, 5-HTR cells in experimental gastric ulcer group were higher than control group on the 4day,10day after operation.The peak of the numerical densities on area of ? EP and 5-HT cells appeared on the 4th day, while the peak of 5-HTR cells as on the 10th day. Part of ? EP, 5-HT,5-HTR immunoreactive material were located in A or B cells neighbourly section. Conclusion The present work proved that the ? EP, 5-HT,5-HTR cells of pancreatic islets were directly or indirectly participated in the regulation balance of endocrine during the self-healing process of the experimental gastric ulcer and here we provided morphological evidence.;

Objective To study physiological foundation of acupunctureDeqi.Methods 28 healthy volunteers who never experienced acupuncture were recruited. The right Zusanli (ST36) and a site 1.5 cm beside Zusanli (ST36) (nominated as A) were acupunctured at different time. Shallow and deep acupuncturing was inserted at Zusanli (ST36). Only deep acupuncturing was inserted at A. Hemodynamic parameters of the anterior tibial artery including the peak value in the systolic phase (Vmax), the average blood flow velocity (Vm), and the inner diameter of vessels (Dist) were observed with colored Doppler ultrasound before, at the end of acupuncture, and 3 min afterDeqi response. Results There was significant difference in those parameters between before acupuncture and 3 min after deqi response when deep acupuncturing at Zusanli (ST36) (P＜0.05), but was not significant difference when shallow acupuncturing (P＞0.05). There was significant difference in ΔVmax and ΔVm between acupuncture at Zusanli (ST36) and A points (P＜0.05). Conclusion Arterial haemodynamics variation seems to be associated with acupunctureDeqi.

Objective To evaluate the value of color Doppler ultrasonography for diagnosis of superior mesenteric artery diseases. Methods78 patients with abdominal pain suspected to be superior mesenteric artery diseases underwent color Doppler ultrasonography. The results were compared with other imaging methods or findings in operation. Results4 patients with superior mesenteric artery embolization coincided with the founding in operation. 18 of the 22 patients suggested with superior mesenteric artery stenoses were confirmed by CTA or DSA. ConclusionColor Doppler ultrasonography can be used in diagnosis of superior mesenteric artery diseases.