Turner Syndrome (TS) is a common X sex chromosome abnormality syndrome. Its main clinical features are growth retardation and germinal aplasia. The study found that the incidence of thyroid abnormalities in patients with TS was higher than that in the general population. This article reviews the clinical features and mechanisms of thyroid abnormalities in patients with TS.

Objectives:To observe the effect of intrahepatic cholestasis on the development and growth of fetus in pregnant rats. Methods: The animal models of intrahepatic cholestasis were made by injecting benzogynestryl (5 mg/kg body weight) or cholate (2.4 mg/kg body weight) for 5 days starting from 16th day of pregnancy. Biochemical parameters of fetal plasma and amniocent were determined at the 21st day of pregnancy. The pathological lesion of placenta in rats was also observed. Results: Cholic acid group showed lower body weight and high level of estrodiol in fetal plasma with high metaphase mortality of fetus. The level of TBA in amniocent was high in estrodiol group compared with normal control group. Pathologically, placenta edema and chorion denaturalization and necrosis with infiltration of neutrocyte were observed in both groups. Conclusions: Intrahepatic cholestasis is detrimental to the development and growth of fetal rats in pregnancy rats.

objective To understand the clinical features of type 2 diabetes mellitus (T2DM) combined with metabolic syndrome (MS) in elderly patients, as well as the medicine used for treatment and the distribution of abnormal metabolic components. Methods Totally 349 T2DM patients admitted from 2001 to 2005 were retrospectively studied, among them 194 patients were combined with MS. The clinical data of those 194 patients were analyzed to evaluate their clinical feature, medicine used and the distribution of several abnormal metabolic components. of T2DM combined with MS. Results The proportion of MS in the elderly T2DM patients was 55.6%. Body mass index (BMI), postprandial blood glucose (PBG), blood pressure and triglyceride (TG) were higher than that in those patients of T2DM without MS, while the high density lipoprotein cholesterol (HDL-C) was lower (P

Objective To investigate the effects of Tong Xin-Luo on cultured human umbilical vein endothelial cells (HUVECs) impaired by Lysophosphatidylcholine.Methods The herbage-contained serum of TXL was prepared,HUVECs were cultured in vitro.The study was designated to 4 group:normal control,LPC group,TXL group,and TXL + LPC intervened group.The cell function was determined by cell morphology and MTT colorimetric assay.Results Compaired with normal control group (0.380 ±0.023 ),LPC ( 0.320 ± 0.024 ) could significantly decrease the cells activity,promote cells death ( P ＜0.05 ).After TXL intervened(0.424 ±0.034),cells activity was significantly increased,cells death was d significantly decreased( P ＜0.05 ).Conclusions Tong Xin-Luo could protect human umbilical vein endothelial cells function by against the LPC-induced damage.

Objective To investigate the distribution and expression of trefoil factor 3(TFF3) in the eosinophilic cells and basophilic cells in the pars distalis of the rat pituitary .Methods The immunohistochemiscal staining technique was used to show the coexpression of TFF3/growth hormone (GH),TFF3/prolactin (PRL),TFF3/thyrotroph (TSH), TFF3/adrenocorticotropin (ACTH),TFF3/follicle stimulating hormone (FSH),TFF3/luteinizing hormone (LH) in two contiguous slices .Results The immunoreactive products of TFF 3 and chromophil cells were brown granules , mainly expressed in cytoplasm .ACTH positive signals were expressed in the cell membrane and mainly located in the pars distalis hypophyseos.TFF3 existed in parts of GH,PRL,TSH,ACTH,FSH and LH cells in contiguous slices, accounting for 19.4%, 22.4%, 9.2%, 6.5%, 35.7%, 8.3%, respectively , in which FSH was the most , PRL and GH were less . Conclusion TFF3 expresses in GH, PRL, TSH, ACTH, FSH, and LH cells in the pars distalis , which enriches the morphological data for the location of TFF 3 in gland cells of pars distalis hypophyseos .

Objective To explore the value of brainstem auditory evoked potentials (BAEP) in the evaluation of spastic cerebral palsy (CP). Methods Forty five cases of CP children were recruited and divided, according to the diagnosis standards, into three groups: diplegia, hemiplegia and tetraplegia groups. Twenty three healthy children served as control. BAEP was recorded from all the subjects and comparisons were done among the various groups. Results The abnormality rate of BAEP in the hemiplegia and tetraplegia groups was significantly higher than that in the diplegia group ( P

AIM: To study the effect of trichosanthin (TCS), a type I ribosome-inactivating protein, on the induction of apoptosis in human leukemic cell line HL-60 cells and the influence of cycloheximide (CHX) on TCS-induced apoptosis. METHODS: Flow cytometry together with fluorescent microscopy were adopted to investigate the apoptotic cell death in HL-60 cells treated with TCS. RESULTS: Flow cytometric analysis indicated that TCS was able to induce significant apoptosis in HL-60 cells. The rates of apoptotic cells in HL-60 cells treated with TCS (20 mg/L) for 48 h was 48.7%±2.3%(±s), which was significantly higher than that of control (6.3%±1.0%)(P＜0.05). Under the same condition, the rate of apoptosis caused by CHX (5 mg/L) was 65.3%±3.9%. TCS-induced apoptosis was further confirmed by fluorescent microscopy observation and DNA gel electrophoresis, in which typical nuclear morphological changes such as chromatin condensation, nuclear fragmentation, were observed in many of the cells treated with TCS， and DNA extracted from these cells displayed typical ladder pattern. Furthermore, the effect of TCS was significantly enhanced with the pretreatment of CHX (0.2 mg/L) which did not induce any significant apoptosis when used at 0.2 mg/L seperately. TCS-induced apoptosis was time- and dose-dependent. CONCLUSION: TCS was able to induce apoptosis in HL-60 cells, which was enhanced by CHX. It was suggested that TCS-induced apoptosis was independent of new protein synthesis.

AIM: To investigate the influences of protein kinase C(PKC) inhibitors on the expression of interleukin-2(IL-2) and interferon-?(IFN-?) by in vitro activated T-lymphocytes. METHODS: Double fluorescent staining together with flow cytometry was adopted to detect intracellular cytokines and to analyze the effects of H7 and gossypol on IL-2 and IFN-? expression levels of T-lymphocytes stimulated with phorbol ester (PDB)+ionomycin(I) in the presence of monensin.RESULTS: The expression rates of IL-2 and IFN-? of CD3 + T cells stimulated with PDB+I for 4 h were 16.64?2.04 and 25.81?3.53( ?s ), respectively, which were significantly higher than that of control (1.06?0.22 and 3.12?0.77)( P

Hydroxyapatite (HAP) formation on the surface of the degradable polyamides implant by a coating technology was studied. A suspension of HAP-glutin-acetic acid was prepared and brushed on the surface of the implant at room temperature. Scanning electron microscopy (SEM), X-ray diffractometry (XRD) and Fourier transform infrared spectroscopy (FT-IR) were employed to study the composition and the microstructure of the coating. The results obtained indicate that the main composition of coating is crystal HAP, its structural formula is Ca10(PO4)6(OH)2. So the coating has bioactivity. The coating has also small pores, which can stimulate bone regrowth and adsorption of other bioactive materials or mediine. This technology is an effective method to improve medical performance of the polyamides implant.
Bone Substitutes ; Coated Materials, Biocompatible ; chemical synthesis ; chemistry ; Durapatite ; chemical synthesis ; chemistry ; Nylons ; Prostheses and Implants

In order to construct a novel multifunctional targeting nano-carrier based on pH-redox characteristics of tumor microenvironment,ketone bonds and disulfide bonds were bonded to the oligomeric hyaluronic acid (oHA) being sensitive to pH and the reduction environment.The chemical structure of oligomeric hyaluronic acid-8-mercaptomenthone 1,2-glycerolketal (oHMST) was characterized by 1H NMR,IR and ESI-MS.Curcuminloaded micelles were prepared by dialysis.The single factor investigation was carried out on the dosage form.Some properties,including particle size zeta potential,the morphology of micelles,and pH-sensitivity were studied.The materials were synthesized successfully.The micelles were spheric with a diameter of about 100 nm.The Zeta potential of the micelles was-(21.97 ± 1.08) mV.The in vitro test showed that oHMST carriers have good pH-sensitivity and redox-sensitivity.