ObjectiveTo make known the role of family health worker in the prevention and treatment of chronic diseases.MethodsIn accordance with the inclusion criteria strictly,146 residents were recruited as samples by convenience sampling.These residents were trained with 《chronic disease family prevention guide》 provided by Beijing City Service Center,special topic discussion,peer education,and self-study.Family health worker certificate were granted for those qualified..SPSS software was used for data analysis.Results There was statistical significance,when the data was compared before and after the training program.ConclusionThe training program improved chronic disease prevention knowledge level on different extent.

[Objective] To explore the mechanism of its formation of sublingual varix in patients with primary liver cancer(PLC) and from the vessels and haemodynamics so as to establish the basis for its future clinical study.[Methods] The related indexes of the haemodynamics vessels tongue and the portal system were measured by Doppler ultrasound.Then the mechanism of the formation of abnormal sublingual varix was explored from the local microcirculation of tongue,the morphology and haemodynamics of the tongue vessels and portal system.[Conclusion]The formation of abnormal sublingual varix was related to haemodynamics of the tongue,and the abnormal morphology and haemodynamics of portal system.The deep mechanism need to be further explored.

Objective To explore the effects of psychological health education on mental health status of middle school students. Methods All middle school students were randomly divided into 2 groups by cluster sampling, a interventional group and a control group. 28 students in the intervention group received group psychological consultation for two months, 30 students in the control group received no therapies.Changes of active respond and negative response subscales(ARNRS), communication anxiety scale (IAS),accommodate others scale(AOS), self-rating depression scale(SDS)were compared before and after the intervention in both groups. Results After group counseling, ARNRS, IAS, AOS, SDS in the interventional group was significantly changed compared with both pre-counseling and the control group, with P<0.05～0.0001 and P<0.05～0.0001, respectively. Conclusion Community mental health education can enhance middle-school student's psychological health level.

Objective:To establish the acute rejection model of orthotopic liver transplantation in rat(ROLT) and observe the basic pathophysiologic changes of acute rejection.Methods:The rats were randomly divided into three groups:control group,isotransplantation group(LEW-LEW),and allotransplantation group(LEW-BN).Recipients were sacrificed on 3,5,7,and 10 days postoperatively and liver tissues and blood samples were collected.Recipient survival rate,histopathological and ultrastructural characteristics were observed.ALT,TBIL,and Alb were measured with automatic biochemical analyser.IL-2 content in serum was assayed by ELISA.Results:There was no rejection in isotransplantation from Lewis to Lewis rat resulted and 14-day survival rate reached 100%.The IL-2 concentration in serum was at normal level.On the contrary,all recipients in allotransplantation group from LEW to BN rat died among 14 days postoperatively,and hepatic histological examination showed typical acute rejection on 7 days.Liver function was severely impaired,which was indicated by significant increase of ALT and TBIL levels and apparent decrease of Alb level.The IL-2 concentration in serum was continuously increased and reached its peak value on 7 days postoperatively.Conclusion:An acute rejection experimental model of liver transplantation in rat could be stably established using Lewis rat as donor and BN rat as recipient.

OBJECTIVE To investigate the genotype characteristics of extended-spectrum ?-lactamases(ESBLs) producing Escherichia coli and Klebsiella pneumoniae and drug resistance condition in Tianjin.METHODS A total of 218 strains of ESBLs-producing E.coli and K.pneumoniae were isolated from clinic.The drug-resistant CTX-M-1,CTX-M-2,CTX-M-8,CTX-M-9,TEM,SHV,OXA-1,OXA-2 and OXA-10 were tested by SYBR GREEN Ⅰ real-time fluorescent quantitative Polymerase chain reaction(PCR).RESULTS Among 109 strains of ESBLs-producing E.coli,the drug resistance of genotypes CTX-M-1,CTX-M-2,CTX-M-8,CTX-M-9,TEM,SHV and OXA-1 was 32.1%,0.9%,0.9%,33.9%,73.4%,27.5% and 15.6%,respectively.The strains with more than two genes were 66.1%.The positive rate of CTX-M-1,CTX-M-2,CTX-M-8,CTX-M-9,TEM,SHV,OXA-1 and OXA-10 was 49.5%,2.8%,1.8%,22.9%,78.9%,76.1%,33.0% and 0.9%,respectively.The strains with more than two genes were 82.2%.The strains were highly sensitive to imipenem.CONCLUSIONS Most of the genotypes of ESBLs-producing E.coli and K.pneumoniae in Tianjin are SHV,TEM,CTX-M-1 and CTX-M-9.The rate of OXA are increasing,and the drug resistance genes of K.pneumoniae from different hospitals are different.

Objective To investigate whether the apoptosis-2 ligand (Apo-2L),known as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL),could enhance irradiation-induced apoptosis in lung adenocarcinima H1975 cells that are resistant to the epithelial growth factor receptor (EGFR)-TKI.Methods Adenocarcinima H1975 cells were randomly divided into four groups:the control group,Apo2L group,irradiation group,and both Apo-2L and irradiation group.H1975 cells were pretreated with Apo2L under different concentrations of 200 and 228 ng/ml at 24 h before irradiation with doses of 1,1.5,2,2.5,3,3.5 and 4 Gy.The apoptosis rates of all groups were analyzed by flow cytometry 24 h post-irradiation.The inhibition rates of cell proliferation were measured by the MTT assay.Results MTT assay showed that the Apo-2L treatment significantly inhibited cell proliferation(x2 =136.17,P ＜ 0.05).The apoptosis rates of the four groups were different significantly,and the apoptosis rate of radiation combined with drug group was significantly higher than the other three groups(x2 =78.02,P ＜0.05).Conclusions The Apo-2L could not only inhibit the proliferation but also promote radiation-induced apoptosis of adenocarcinoma H1975 cells.

The paper introduces our hospital as a former military hospital in the system conversion process.In order to adapt to administration of Guangdong Province,the first page of medical record program of hospital information systems is developed.

Objective To investigate the relationship of parity and other reproduction-associated factors with metabolic syndrome(MS) in middle-aged and elderly women in Nanchang community.Methods Female permanent residents over 40 years old were sampled for a routine examination and a questionnaire survey covering basic information,5 894 subjects were enrolled and identified as effective cases.MS was diagnosed according to the standard of China Diabetes Society in 2004.Factors including age,education,profession,marital status,parity,child-bearing history of macrosomia,breastfeeding,menarche age,and menopausal age were analyzed in order to find their relationship with MS.The univariate logistic analysis was followed by binary logistic regression analysis if P＜0.05,and OR value and confidence interval were calculated.Results Univariate logistic regression analysis showed that age,education,profession,parity,breastfeeding,and menopausal age were influencing factors of MS (all P ＜ 0.05).There was no correlation to marital status,miscarriage history,child-bearing history of macrosomia,menarche age with MS(all P＞0.05).Binary Logistic regression analysis showed that the risk of MS increased with age:OR =1.51 (95 ％ CI 0.98-2.32) during 50-59 years,OR =2.10 (95 ％ CI 1.34-3.30) during 60-69 years,and OR =2.63 (95 ％ CI 1.61-4.31) in cases aged over 70 years,as compared to the group aged 40-49 years.The risk in non-manual workers increased about 34 percent OR =1.33 (95 ％ CI 1.04-1.70),compared with manual workers.The risk of MS increased with parity,being OR =1.46 (95％ CI 1.14-1.88),OR =1.78 (95％ CI 1.30-2.43),and OR =1.84 (95％ CI 1.24-2.72),with 2,3,and 4 child-bearings respectively as compared to cases with only one childbearing.The risk of MS was reduced with advancing menopausal age being about 6 percent reduction with each increased year of menopausal age(OR =0.94,95％ CI 0.90-0.99).There was no difference between education,breastfeeding and MS(P＞005).Conclusion Multiple parity,earlier menopausal age,less manual work,and aging seem to be the risk factors of MS in women.

Objective:To develop an HPLC method for the simultaneous determination of four phenolic compounds ( monocaffey-ltartaric acid, chlorogenic acid, caffeic acid and chicoric acid) in Herba Taraxaci. Methods:The separation was performed on a Ther-mo C18 column (250 mm × 4. 6 mm, 5 μm) with methanol-0. 2％ phosphonic acid as the mobile phase with gradient elution. The de-tection wavelength was 328 nm, the flow rate was 1. 0 ml·min-1 and the column temperature was 35℃. Results:The linear range of monocaffeyltartaric acid, chlorogenic acid, caffeic acid and chicoric acid was 11.59-115.90 (r = 0.9999), 2.50-24.96(r =0. 9998),2. 27-22. 70(r=0. 9995) and 12. 44-124. 40(r=0. 9998) μg ·ml-1, respectively. The average recovery was 102. 50％(RSD=2. 30％), 99. 29％(RSD=0. 43％), 96. 71％(RSD=0. 78％) and 95. 36％ (RSD=1. 30％), respectively. Conclusion:The method is simple and accurate with good repeatability, which can be used for the quality control of Herba Taraxaci.

Objective To discuss the clinical effects of arthroscopy combined with Brostrom repair in the treatment for chronic ankle instability talofibular ligament injury.Methods From January 2012 to June 2015,42 cases of chronic ankle instability in our hospital were performed modified Brostrom repair.All the cases were evaluated by AOFAS scoring system.Results All the patients were followed up for 9 to 17 months,an average of (12.7±3.5)months.At the last follow-up,all cases could participate in normal daily running training mission.Activity levels of 2 cases with cartilage injury had a decline than before.The others were able to attend the confrontational training.Postoperative AOFAS score(88.4±4.9) increased compared with preoperative score (43.3±7.8),the difference was significant(P<0.05).ConclusionThe curative effect of arthroscopy combined with Brostrom repair in treatment of chronic ankle instability talofibular ligament injury is satifactory.It can be helpful to the wounded rapid rehabilitation and good for the popularization and application in basic-level hospitals.