Objective To study the effects of Pinacidil on Ventricular Repolarization in LQT2 Rabbit Heart.Methods 30 New Zealand white rabbits weighted from 2.5~3.0 kg were randomly divided into 3 groups:group A(control),group B(pinacidil),group C(pinacidil + glibenclamide).Left ventricular endocardium and epicardium MAPs and volume-conducted ECGs in isolated Langendorff-perfused rabbit hearts were recorded simultaneously.To induce bradycardia,the AV nodes of rabbit hearts were damaged.EAD and TdP were induced by means of bradycardia in the presence of high concentration of d-sotalol(10-4M).Results In group B,pinacidil(5?mol/L)shortened the APD90 on endocardium from 445.48?54.31ms to 278.87?44.45ms after administration of pinacidil for 5 minutes(P0.05 vs.group A for all).Conclusion Pinacidil can decrease prolonged action potencial duration and TDR induced by bradycardia and high concentration of d-sotalol in rabbit hearts.Also,pinacidil could abolish the EAD and TdP related to delayed repolarization that may provide a novel and useful intervention in the clinical LQTS patients.

Objective To investigate the characteristic of top-down attentional control of acute stress disorder children.Methods According to SCID-IV-TR,23 acute stress disorder children were chosen as the experinent group and 23 normal children were chosen as the control group.They were asked to perform a visual search task.Results (1) The reaction time of acute stress disorder children((1 253±158)ms) was significantly longer than normal children's((1 194± 146) ms) (P＜0.05).(2) The reaction time of valid condition((1 172± 144) ms)was significantly shorter than neutral condition ((1 229± 156) ms) and invalid condition ((1 269± 157) ms) (P＜0.05),there was no difference of reaction time between neutral condition((1 229± 156)ms) and invalid condition ((1 269±157)ms) (P＞0.05).(3) The reaction time of simple display condition((1 182±127)ms) was significantlv shorter than complex display condition ((1 264± 177)ms) (P＜0.01).Conclusion The performance of acute stress disorder children on top-down attentional control is less than normal children,the reason is that inhibiting capacity of acute stress disorder children is lower than normal children.It indicates that trauma event have negative influence on children's inhibiting capacity.

Objective:To establish an HPLC coupled with post column derivatization method for the determination of gentamicin C components and the related substances based on the latest European Pharmacopeia and compare with the electrochemical method. Methods:A Hydrophilic C18(250 mm ×4.6 mm, 5 μm)column was used with acetonitrile-50 mmol·L-1 sodium hydroxide solution ( pH 2. 6) containing 0. 7% trifluoroacetic acid and 0. 025% pentafluoropropanoic acid (1. 5∶98. 5) as the mobile phase. The temper-ature of post-column reaction was set at 30℃, and the samples were detected by a fluorescence detector withλex of 340nm andλem of 430nm. A pulsed amperometric detector (PAD) was applied in the electrochemical method with golden working electrode in a four-po-tential working mode. Results: According to the results of the two detection methods, the linear range of C1a , C2 , C2a and C1 was 5.82-233.00,6.92-277.00,4.00-160.00and6.23-249.00 μg·ml-1(r >0.9993) , respectively. The limit of detection and quantization were 0. 92-3. 28ng and 1. 37-5. 19ng, respectively. Conclusion:There is no significant difference between the determina-tion results of the two methods.

ObjectiveTo find out the incidence of triple-negative breast cancer(TNBC) in all kinds of breast cancers.To compare and analyze the clinicopathological features,recurrence,metastasis,and prognosis of patients with TNBC and non-triple negative breast cancer (non-TNBC).MethodsThe clinicopathological features and follow-up data of 387 patients with primary breast cancer histopathologically conffirmed in our hospital from Sep.2004 to Sep.2006 were retrospectively analyzed.The 387 patients were divided into 2 groups:79 cases of TNBC and 308 cases of non-TNBC.The clinical features and prognosis of the 2 groups were compared.Results Compared with non-TNBC group,patients in TNBC group had their special features:1.higher ratio of patients ＜ 35 years( P =0.012 ) ; 2.higher ratio of patients with family history of breast cancer( P =0.031 ) ; 3.higher ratio of tumors with maximum diameter ≥ 5 cm ( P =0.044 ) ; 4. higher ratio of patients with positive lymph nodes(P =0.011 ) ; 5.higher ratio of tumors in clinical stage Ⅲ(P =0.007) ; 6.higher ratio of tumors in histological stage Ⅲ(P =0.028 ).The 5-year-disease-free survival (DFS) and overall survival (OS) rate for patients with TNBC were 72.15％ and 88.61％ respectively,lower than those of non-TNBC ( P =0.003 and 0.031 respectively).ConclusionsCompared with non-TNBC patients,patients with TNBC have the features of younger age,more advanced clinical stage upon diagnose,higher rate of lymph node metastasis,larger tumors,higher histological grade,faster and easier recurrence and metastasis,and lower rate of DFS and OS.The information of age,the maximum diameter of the tumor,lymph node status,clinical stage,histological grade and pathological types,especially the age and lymph node status,play an important role in predicting the prognosis of TNBC.

Objective:To establish a method for the determination of sodion in cefalotin sodium by ion chromatography and investi-gate the salt-forming rate of the products. Methods: A TSKgelSuper IC-CR cation exchange column (150 mm × 4. 6 mm, 3. 0 μm) was used. The mobile phase was the mixture of 2. 2 mmol·L-1 methanesulfonic acid and 1 mmol·L-1 18-crown-6-ether with the flow rate of 0. 8 ml·min-1 . The column temperature was 40℃ and the injection volume was 20μl. The detector was an electric conductiv-ity detector. Results:The linear correlation of sodion was good within the range of 3. 0-60. 0μg·ml-1(r=0. 999 9). The average re-covery was 99. 8%(RSD=0. 8%, n=9). The mole number ratio of sodion to cefalotin was within the range of 0. 97-1. 03. Conclu-sion:The method is specific, precise and accurate, and can be used in the determination of sodion in cefalotin sodium. The salt-form-ing rate of the 8 batches of samples is promising.

This article combs and summarizes the entire process of rare disease selection and priority theme determination,including the application and preliminary review of rare diseases,standardization of disease theme information,the evaluation methods of evidence sorting and disease selection for priority se-lection of disease themes,and other aspects of the content were analyzed in depth.It is expected to provide reference for the subsequent selection of rare diseases,improve the fairness,rationality and scientificity of rare disease selection,and further promote research and decision-making in China's rare disease-related fields.

Objective To investigate the relationships of serum levels of human fractalkine(CX3CL1)and chitinase-3-like protein 1(YKL-40)with early cognitive impairment in elderly patients with Alzheimer's disease(AD).Methods A total of 110 AD patients in the Second Affiliated Hospital of Xinxiang Medical University from February 2021 to December 2023 were selected as AD group,and 50 healthy individuals with physical examination during the same period were selected as control group.Clinical materials and serum levels of CX3CL1 and YKL-40 were compared between the two groups,and multivariate Logistic regression models were used to analyze the influencing factors of cognitive im-pairment in AD patients.Based on the Mini-Mental State Examination(MMSE)score,the 110 AD patients were divided into mild cognitive impairment group(n=47),moderate cognitive impairment group(n=36),and severe cognitive impairment group(n=27).Spearman correlation analysis was performed to explore the relationships of serum CX3CL1 and YKL-40 with MMSE score,Addenbro-oke's Cognitive Examination-Ⅲ(ACE-Ⅲ)score,and Montreal Cognitive Assessment(MoCA)score.Results The AD group had higher proportions of patients aged over 80 years,with an edu-cation level of primary school or below,smoking history,alcohol consumption history,diabetes mel-litus,hypertension,AD family history,and living alone as well as higher serum levels of CX3CL1 and YKL-40 compared to the control group;conversely,the AD group had a lower proportion of pa-tients engaging in no physical exercise/labor,and lower MMSE,ACE-Ⅲ,and MoCA scores,with significant between-group differences(P＜0.05).Advanced age,diabetes mellitus,and high ser-um levels of CX3CL1 and YKL-40 were independent risk factors for cognitive impairment in AD pa-tients(P＜0.05),while an education level of college or above was a protective factor(P＜0.05).Compared with the mild cognitive impairment group,the moderate and severe cognitive impairment groups had higher serum levels of CX3 CL1 and YKL-40,and the severe cognitive impairment group had higher serum levels of CX3 CL1 and YKL-40 than the moderate group,with significant between-group differences(P＜0.05).Spearman correlation analysis revealed that serum CX3CL1 and YKL-40 were negatively correlated with MMSE,ACE-Ⅲ,and MoCA scores(P＜0.05).Conclu-sion Serum CX3CL1 and YKL-40 are highly expressed in elderly AD patients,and are closely re-lated to early cognitive impairment in elderly AD patients.

Objective To investigate the relationships of serum levels of human fractalkine(CX3CL1)and chitinase-3-like protein 1(YKL-40)with early cognitive impairment in elderly patients with Alzheimer's disease(AD).Methods A total of 110 AD patients in the Second Affiliated Hospital of Xinxiang Medical University from February 2021 to December 2023 were selected as AD group,and 50 healthy individuals with physical examination during the same period were selected as control group.Clinical materials and serum levels of CX3CL1 and YKL-40 were compared between the two groups,and multivariate Logistic regression models were used to analyze the influencing factors of cognitive im-pairment in AD patients.Based on the Mini-Mental State Examination(MMSE)score,the 110 AD patients were divided into mild cognitive impairment group(n=47),moderate cognitive impairment group(n=36),and severe cognitive impairment group(n=27).Spearman correlation analysis was performed to explore the relationships of serum CX3CL1 and YKL-40 with MMSE score,Addenbro-oke's Cognitive Examination-Ⅲ(ACE-Ⅲ)score,and Montreal Cognitive Assessment(MoCA)score.Results The AD group had higher proportions of patients aged over 80 years,with an edu-cation level of primary school or below,smoking history,alcohol consumption history,diabetes mel-litus,hypertension,AD family history,and living alone as well as higher serum levels of CX3CL1 and YKL-40 compared to the control group;conversely,the AD group had a lower proportion of pa-tients engaging in no physical exercise/labor,and lower MMSE,ACE-Ⅲ,and MoCA scores,with significant between-group differences(P＜0.05).Advanced age,diabetes mellitus,and high ser-um levels of CX3CL1 and YKL-40 were independent risk factors for cognitive impairment in AD pa-tients(P＜0.05),while an education level of college or above was a protective factor(P＜0.05).Compared with the mild cognitive impairment group,the moderate and severe cognitive impairment groups had higher serum levels of CX3 CL1 and YKL-40,and the severe cognitive impairment group had higher serum levels of CX3 CL1 and YKL-40 than the moderate group,with significant between-group differences(P＜0.05).Spearman correlation analysis revealed that serum CX3CL1 and YKL-40 were negatively correlated with MMSE,ACE-Ⅲ,and MoCA scores(P＜0.05).Conclu-sion Serum CX3CL1 and YKL-40 are highly expressed in elderly AD patients,and are closely re-lated to early cognitive impairment in elderly AD patients.