1.Effect of application of clinical nursing pathway in patients undergoing transurethral resection of prostate
Chinese Journal of Practical Nursing 2008;24(30):31-32
Objective To study effect of application of clinical nursing pathway in patients undergoing transurethral resection of prostate. Methods Clinical nursing pathway was established accorded with patients undergoing transurethral resection of prostate.Patients admitted before the application of clinical nursing pathway were set as the control group,while those admitted after the application of clinical nursing pathway were set as the experimental greup.The hospitalization time,medical cost, mastering degree of disease-related knowledge and satisfaction degree were compared between the two groups. Resets The hoapitalization time was shorter, the medical cost was lower, the mastering degree of disease-related knowledge and satisfaction degree were improved in the experimental group compared with those of the control group, P < 0.05. Conclusions Application of clinical nursing pathway in patients undergoing transurethral resection of prostate can improve nursing quality and reduce the economic burden of patients.
2.The role of telomerase reverse transcriptase in monocrotaline-induced pulmonary hypertension in rats
Chinese Journal of Pathophysiology 1986;0(03):-
AIM and METHODS: To explore the role of telomerase reverse transcriptase (TERT) in monocrotaline-induced pulmonary hypertension (PH), TERT was investigated by using immunohistochemistry and image analysis on the chronic PH induced by MCT in Sprague-Dawley rats. RESULTS: TERT was positive in pulmonary vascular smooth muscle cells, bronchial smooth muscle cells and chondrocytes in normal group. TERT in MCT group was more positive in pulmonary vascular smooth muscle cells and bronchial smooth muscle cells compared with normal group. CONCLUSION: Over expression of TERT in pulmonary vascular smooth muscle cells and bronchial smooth muscle cells is related to MCT-induced PH.
3.The pathological,immunohistochemical and ultrastructural features in primary gastric malignant lymphoma.
Fulin SONG ; Jingchun ZHOU ; Xue LI
Chinese Journal of Practical Internal Medicine 2000;0(11):-
Objective To investigate the clinicopathological features of primary gastric malignant lymphoma.Methods 32 cases of primary gastric non Hodgkin's malignant lymphoma(NHL)were studied by clinicopathology,immunohistochemistry and ultrastructure.Results 3 cases of NHL arose from fundus of stomach,7 cases from body of stomach,8 cases from gastric angle,and 14 cases from gastric antrum.The paraffin sections of all cases were stained.There were 31 cases(96\^9%)exhibiting B cell phenotype,only 1 case(3\^1%)exhibiting T cell phenotype.11 cases of NHL and 6 cases of undifferentiated carcinoma(UC)in stomach were observed comparatively under electron microscope(EM).The ultrastructural characteristics of NHL were much different from those of UC.Conclusion Most primary gastric malignant lymphoma are B cell lymphomas.Immunohistochemistry and ultrastuctural observation are much helpful in the diagnosis and differential diagnosis between NHL and UC.
5.Clinical cases discussion:fever-hemoptysis-chest pain-pleural effusion
Yuan ZHANG ; Jingchun HE ; Liyu LI ; Dongqing LI ; Likui QIAO
Chinese Journal of Geriatrics 2014;33(9):1025-1028
This report presented a male patient aged 69 years,who was admitted into our hospital for fever,hemoptysis and chest pain.Chest X-Ray showed shadows on the right lung and pleural thickening.The effect of broad spectrum antibiotic therapy was poor.With the disease progressed,pleural effusion appeared on the right side.Blood culture showed methicillin-resistant staphylococcus aureus.Pleural effusion tests indicated pyothorax,but the effects of closed chest drainage and sensitive antibiotic therapy were poor.After disentangling with open thoracic exploration,the right middle lobe was resected and the right lung lobe pathology showed Wegener granulomatosis.His blood antineutrophil cytoplasmic autoantibodies was negative.The paranasal sinus CT scan and renal function showed no abnormalities.The definitive diagnosis was pulmonary limited Wegener granulomatosis.
6.The clinical study of transrectal ultrasound guided biopsies of the prostate cancer with PSA level less than 4 μg/L
Li XU ; Jie TANG ; Jingchun YANG ; Shunxin ZHANG ; Yanmi LI
Chinese Journal of Ultrasonography 2008;17(9):779-781
Objective To evaluate the clinical value of transrectal ultrasound(TRUS)guided biopsies of the prostate cancer(PCA)with serum prostate specific antigen(PSA)level less than 4 μg/L.Methods Fifty-nine patients suspected PCA were underwent TRUS guided-biopsy.The ultrasonographic features of lesions and prostate gland were observed.The PSA,PSA density(PSAD),F/T(free/total PSA ratio),prostatic volume(PV)were compared between PCA group and non-PCA group,and further analyzed the sensitivity and specificity of PSAD and F/T at different thresholds for PCA diagnosis.Results In 59 patients,16 were proved to be cancer by pathological examination,the positive detection rate was 27%.In these 16 patients,12 had lesions on ultrasound,which locatqed in outer gland.The rich vascularity was observed in PCA more than in benign and precancerous lesion.There were statistical significant differences in PSA,PSAD,F/T,PV between PCA and non-PCA groups.When selecting threshold of PSAD and F/T were 0.09 and 0.20,respectively,there were the highest diagnostic specificity and sensitivity.The diagnostic specificity and sensitivity of PSAD were higher than those of F/T.Conclusions TRUS guided biopsies of the prostate is one of the most efficient methods for detection of PCA with PSA level less than 4μg/L.Combining TRUS features with PSA parameters can further increase positive detection rate of PCA.
7.Effect of Yujin on Cerebral Functions of Acute Hypoxia Mice
Zonghua LI ; Feng LI ; Jingchun HE ; Xin GUO ; Lijuan ZHAO
Chinese Journal of Rehabilitation Theory and Practice 2007;13(8):710-711
Objective To observe the effect of Yujin on cerebral function of acute hypoxia mice.MethodsThe acute hypoxia mice model was reproduced by cutting heads and establishing normal-pressure and closed hypoxic experimental settings. In yujin groups, the mice were treated with Yujin at the doses of 10 g/kg, 20 g/kg, 40 g/kg respectively through the intragastric tubes everyday. 6 days later, the mice survival time, the open-mouth times after cutting heads and the breathing time were observed. The activity of superoxide dismutase (SOD) and content of maleic dialdehyde (MDA) in cerebral tissues were measured. The morphological changes of the cerebral tissues under the microscope were examined.ResultsYujin could significantly prolong the survival time ( P<0.001) and the breathing time and increase the open-mouth times ( P<0.05~0.001), increase the activity of SOD ( P<0.001) and decrease the content of MDA ( P<0.01~0.001) in cerebral tissues. Compared with control group, the Yujin group mice had no obvious pathological alteration except some cerebral vascular dilatation.ConclusionYujin has an obvious protective effect on cerebral tissues of acute hypoxia mice.
8.ERK1/2 pathway mediates the protective effect of hydrogen sulfide against cisplatin-induced injury in human marrow mesenchymal stem cells
Haihua GUO ; Gang HUANG ; Jingchun LI ; Jianqiang FENG ; Bicheng YONG
Chinese Journal of Orthopaedics 2014;34(3):323-329
Objective To explore the protection and mechanism of hydrogen sulfide (H2S) preconditioning against injury induced by cisplatin in human marrow mesenchymal stem cells (HMMSCs).Methods HMMSCs were treated by cisplatin at 0,5,10,20,40,60,80 mg/L concentrations for 24 h respectively and were exposed to cisplatin at 20 mg/L concentrations for 0,6,12,24,36,48 h respectively.HMMSCs were pretreated with NaHS at 0,0.4,0.6,0.8,1.0 mmol/L respectively for 30 min before exposed to cisplatin at 20 mg/L concentrations for 24 h.HMMSCs were treated by U0126 or combined with human epidermal growth factor (HEGF) together for 30 min before they were preconditioned with NaHS for 30 min.The cell survival rate,cell apoptosis rate,the expression of p-ERK1/2 and t-ERK1/2 were recorded.Results The cell survival rate decreased to 71.72%±2.72%,59.41%±5.44%,50.37%±4.55%,38.97%±2.92%,30.11%±4.64% and 21.71%±5.35% respectively after cells were treated with cisplatin at 5,10,20,40,60,80 mg/L concentrations respectively,and the differences were statistically significant compared with 0 mg/L group.The cell viability fell to 70.30%±6.20%,61.63%±2.70%,51.29%±3.13%,38.72%±3.66% and 27.57%±2.32% after HMMSCs were treated with cisplatin at 20 mg/L for 6,12,24,36,48 h respectively,and the differences were statistically significant compared with 0 h group.The cell viability increased to 65.99%±2.67%,72.93%±5.44%,75.10%±4.71% and 76.56%± 5.25% when HMMSCs got pretreatment of NaHS,and the differences had statistical significance compared with cisplatin group.The cell apoptotic rate decreased from 35.29%±2.77% to 18.62%±0.97% when HMMSCs were pretreated with NaHS at 0.6 mmo/L.Treatment of HMMSCs with cisplatin at 20 mg/L for 24 h reduced p-ERK1/2 expression.The pretreatment of NaHS could inhibit the inhibitory action to the expression of p-ERK1/2 induced by cisplatin.Pretreatment with U0126 or HEGF inhibited or promoted the protection and the upregulated expression ofp-ERK1/2 caused by NaHS pretreatment.Conclusion The preconditioning of H2S can protect cell damage caused by cisplatin via activating the ERK1/2 pathway of HMMSCs.
9.EFFECT OF SELENIUM ON REGRESSION OF ATHEROSCLEROSIS IN QUAILS
Tong LU ; Jingchun RUAN ; Yun WANG ; Li YOU ; Meiling WANG
Acta Nutrimenta Sinica 1956;0(02):-
The effect of Selenium(Se) on regression of atherosclerosis(AS) induced by feeding quails -with high cholesterol diet was observed. The result indicated that serum cholesterol, triglyceride and lipid peroxide significantly decreased and the ratio of high density lipoprotein cholesterol/total cholesterol increased markedly in Se treated group, that the cholesterol content of aortic tissue and AS index were significantly reduced at the end of the 15th week. It was suggested that Se had a powerful accelerated effect on regression of experimental AS.
10.Effect of endoplasmic reticulum stress in renal injury of fluorosis rats
Jingchun SUN ; Chunyan WANG ; Hui XU ; Guangsheng LI
Journal of Jilin University(Medicine Edition) 2006;0(06):-
Objective To observe the change endoplasmic reticulum stress in renal injury of fluorosis rats,and explore the effect of endoplasmic reticulum stree in the mechanism of renal injury of fluorosis.Methods 48 Wistar rats were divided into 4 groups:control,fluoride-treated,low calcium,and low calcium plus fluoride-treated.The rats in fluoride-treated and low calcium plus fluoride-treated groups were treated with sodium fluoride (NaF,221 mg?L-1) through drinking water for 3 months.The pathological slice of rat kidney was made and the morphological changes were observed under optical microscope.The transcription levels of Grp78,Xbp1,CHOP and PDI in the kidney tissues were determined by reverse transcription-polymerase chain reaction (RT-PCR).Results Edema and vacuolar degeneration of the proximal tubule and distal tubule cells in the kidney tissues in fluoride-treated group were observed;and cell edema,vacuolar degeneration,scattered necrosis,minor regeneration repair and interstitial hyperemia in the kidney tissues in low calcium plus fluoride-treated group were found.The mRNA expressions of Xbp1 in the kidney tissues in fluoride-treated and low calcium plus fluoride-treated groups were markedly higher than that in the responding control groups(P