Objective: To explore the Influence of ethics education on ethical beliefs of dual relationship of trainees who attend training of psychological consultation.Methods: Use of questionnaire,written and directed by "psychological counseling and treatment of ethical issues questionnaire(trainee attending psychological consultation training)" as a tool to evaluate,within the framework of Guangdong 368 trainees to investigate.Results: Compared with two groups of trainees,in refer to the ethical beliefs of dual relationship,the latter group who has a higher option of "not sure" shows varying degrees of decline in most of the items in the questionnaire.Two teams of trainees reach an identity of 80% or more(10 items in total) in the ethical judgment of dual relationship(choose "not").Two groups show significant differences in judging 5 items of the entries(p

According to the overview of historical development of ethical research in psycho-counseling and psycho-therapy in developed countries,this paper aims to summarize the research experience and outcome,in order to promote related Chinese ethical research based on China's realistic situation.

Objective To investigate the status quo of returning to the society of middle-aged and young patients undergoing peritoneal dialysis based on the health empowerment theory. Methods Using the purposive sampling method, 11 patients were interviewed. The interview results were analyzed using Colaizzi analysis and the keywords were extracted. Result A total of 9 keywords were extracted, including beliefs and attitudes ( the sense of responsibility, economic burden and social needs to be met);knowledge and ability ( ability to maintain normal peritoneal dialysis but lack of the ability to solve related complications and decreased work ability after returning to society);related to action and performanc ( limited occupational self-development after returning to society,related to social support and resources:more social support, guidance in chronic disease management and proper social welfare). Conclusions Returning to society can improve the living conditions of middle-aged and youth patients with peritoneal dialysis although they can run into troubles in economic conditions, fatigue, and lack of disease management knowledge and ability. Medical workers should provide them with instructions on disease management knowledge, strengthen social support system other than their families and help them out of the troubles so that they can return to society and enhance their life of quality.

A model of vascular smooth muscle cell proliferation was estabolished by balloon angioplas-ty in rat's aorta. We found that treatment with batroxobin decreased plasma fibrinogen level after angio-plasty, and inhibited the increase in smooth muscle cell count of injured vascular wall and incorporation rate of H-TdR into vascular tissues effectively. The results suggest that batroxobin may have clinically prospective significance in prevention and therapy of angioplasty-induced restenosis.

0.05). (4) The result of detoxincation in- vivo was not as good as that of antiserum.

To put forward spatial resolution detection requirements of 64-slice helical CT based on analyzing the present situation of CT spatial resolution detection. The requirements for spatial resolution in the existing industrial standards were analyzed, and some test data were used to prove the deficiency of the standards. Spatial resolu-tion detection requirements were proposed for 64-slice helical CT, and the factors affecting spatial resolution were ex-plored. The deficiencies in the original standards were eliminated. The proposed requirements may facilitate the spatial resolution detection of 64-slice CT, and thus can enhance image quality for clinical diagnosis and treatment.

Objective To analyze of the relationship between level of anxiety and depression before surgery and anus exhausting time for patients after gynecological abdominal surgery.Methods A descriptive study was conducted with a survey of 100 cases of patients with gynecological abdominal surgery where the level of anxiety and depression before surgery and post-anus exhausting time were analyzed to explore the correlation between them.Results In the 88 valid questionnaires,the incidences of stress and anxiety before surgery were respectively 38.64％(34/84) and 19.32％(17/88) and the average anus exhausting time after surgery was (44.55 ± 13.90) hours;the anus exhausting time of anxious patients was significantly longer than that of non-anxious patients [(48.35±12.84) hours vs.(42.50±14.13) hours,t=-2.12,P＜0.05] and bivariate Logistic regression analysis suggested that preoperative level of anxiety had a significant effect on anus exhausting time after surgery.Conclusions Stress,anxiety and other negative emotions can affect anus exhausting time after surgery and level of anxiety could be used as one predictor of the delay of anus exhausting time after surgery.

Objective:To analyze the expression of E-cadherin in the epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β1 (TGF-β1) in neuroblastoma.Methods:TGF-β1(1 μg/L, 5 μg/L, 10 μg/L), was applied to SK-N-SH cells in vitro compared with the blank control group.EMT-related genes mRNA and protein expression were detected by carrying out real-time PCR assays and Western blot.A scratch test and migration assay were performed to verify the alteration of SK-N-SH cell migration capacity.Data collected from 18 cases of neuroblastoma patients were selected from the Department of Hematology Oncology, Shanghai Children′s Hospital from January 2008 to December 2012.The expression of E-cadherin in the tumor tissue of the neuroblastoma patients after operation was detected by immunohistochemistry.The clinical features and survival prognosis of these patients were analyzed. Results:Compared with the control group, after SK-N-SH cells were treated with TGF-β1(1 μg/L, 5 μg/L, 10 μg/L), real-time PCR assays and Western blot revealed that the mRNA(0.603±0.081, 0.606±0.008, 0.716±0.166 vs.1.000) and protein expression levels(0.855±0.026, 0.600±0.017, 0.495±0.011 vs.1.000) of E-cadherin were significantly decreased ( F=8.144, P=0.040; F=74.810, P<0.001), while the mRNA(2.132±0.167, 3.494±0.017, 4.184±0.021 vs.1.000) and protein expression levels (1.175±0.053, 1.189±0.058, 1.225±0.106 vs.1.000)of α - smooth muscle actin were significantly increased ( F=547.300, P<0.001; F=68.810, P=0.007), suggesting that EMT changes occur in cells.Scratch test and Transwell migration assay revealed that the number of migrating cells increased obvious with the treatment of TGF-β1 (5 μg/L) ( t=16.070, P=0.040). The 10-year overall survival(OS) rates of neuroblastoma patients with E-cadherin strong positive expression, positive expression, weak positive expression and negative expression in the pathology were (77.78±13.86)%, (75.00±21.66)%, (25.00±21.65)% and 0, respectively ( F=8.160, P=0.040). Conclusions:TGF-β1 can induce the EMT in SK-N-SH cells and increase cell migration.The decrease expression of E-cadherin in neuroblastoma patients is closely associated with clinical progress and recurrence or metastasis of the disease.

Objective To systematially observe the different patterns and leves of military stress on modified multitle-platform method the level of stress-related hormones in rats .Methods A sleep deprivation model was established by water environment modified multiple-platform method(MMPM).A restraint stress model was established by self-made bondage cage.Chronic unpredictable mild stress ( CUMS) model was established by action control , cage damp, cage tilt, night lightening, water and food fasting , empty bottles stimulation and group feeding methods .Cortisol, catecholamines and 5-HT levels were detected to observe the different military stress loads and modes on the level of stress hormones .Results The level of serum cortisol increased significantly ( P <0.05 ) after 5 days of sleep deprivation .The level of serum catecholamines increased significantly (P<0.05) after 1 day of sleep deprivation.The cortisol concentration increased and the level of 5-HT decreased in serum after 4 weeks CUMS.The level of catecholamines increased significantly (P<0.05) after 2-8 weeks of CUMS, increased significantly (P<0.05) after 1 week of restraint stress , and returned to normal after 3-4 weeks of restraint stress .The level of serum cortisol increased significantly after 3 -4 weeks of restraint stress . Conclusion Cortisol levels gradually increased with the level of stress in different military stress modes , which can serve as an index to evaluate the level of different stress modes .

Objective To study the possibility of the plasma level of heat shock protein 70(HSP70) being used as a bi-ological marker of military stress .Methods Soldiers who returned from a 6-month-navigation were chosen as subjects , the HSP70 level of plasma was measured with the ELISA assay and stress questionnaires and Self -rated Health Measurement Scale (SRHMS) were used to measure the stress level .Results The soldiers′plasma level of HSP70 was 31.40%higher than that of the control .The stress questionnaire indicatesd that the level of thinking and anxiety , negative mood and somat-ic symptoms were higher than normal .The SRHMS indicated that the level of physiological health ,mental health and social health was lower than normal .The plasma level of HSP70 was associated with the level of military stress .Conclusion The plasma level of HSP70 may be used as an important predictor of military stress .It can predict the level of military stress injury.