Objective To establish a recombinant retroviral vector containing insulin-like growth factor-1(IGF-1) gene and to provide the basis for the application of IGF-1 in treating nervous system diseases such as stroke.Methods The plasmid pcDNA3.1-IGF-1 was cut by EcoR I/Xho I,and subcloned to retroviral vector pLXSN,resulting in the recombinant plasmid(pLXSN-IGF-1.)The recombinant IGF-1 expression vector was evaluated by using enzyme cutting and sequencing.By the Lipofectamine 2000,pLXSN-IGF-1 was transferred to packaging cell line-pA317.Culture supernatant of these cells was detected for titration of the recombinant virus.Results The two fragments from recombined IGF-1 eukaryotic expression vector by EcoR I and Xho I represented 400?bp and 6.0?kb by agarose electrophoresis,and PCR showed positive fragment which was about 400?bp long,and sequence analysis showed the same sequence as expected.The cell line pA317-IGF-1 was established,and average virus titer of the recombinant virus in the culture supernatant was about 6.5?10~5 CFU/ml.Conclusion A recombinant retroviral containing IGF-1 gene was successfully constructed.

Objective To observe the factors affecting inadequate ST-segment resolution in patients with acute ST-segment elevation myocardial infarction after emergency percutaneous coronary intervention (PCI). Methods The patients with acute ST-segment elevation myocardial infarction undergoing emergency PCI were enrolled for study. According to the ratio of ST-segment resolution, 186 cases were divided into inadequate ST-segment resolution group (54 cases) and relatively adequate ST-segment resolution (132 cases). Clinical data of two groups were compared. The single factor and multiple Logistic regression analysis were performed to determine the factors influencing inadequate ST-segment resolution. Results Pre-infarction angina was protective factor of inadequate ST-segment resolution after emergency PCI (OR=0.361, 95%CI 0.131-0.994, P<0.05). Anterior myocardial infarction, attack-to-balloon time, Killip classification and white blood cell counts were risk factors of inadequate ST-segment resolution (OR=2.389, 95%CI 1.194-4.781;OR=1.655, 95% CI 1.082-2.532; OR=1.319, 95% CI 1.026-1.695; OR=1.184, 95% CI 1.004-1.396, P<0.05). Conclusions Patients with pre-infarction angina could reduce the risk of inadequate ST-segment resolution after PCI. Patients with anterior myocardial infarction,long attack-to-balloon time, Killip classification≥2 and high blood cell counts could increase the risk of inadequate ST-segment resolution, and earlier and more active clinical intervention should be taken.

Objective To study the signs and the value of CT enhanced scanning in diagnosing the liver cancer combined arteriovenous fistulae(AVF).Methods 50 cases of the liver cancer combined AVF had plain and enhanced scanning by spiral CT,then proved by hepatic arteriography.The signs of CT and hepatic arteriography were contrast analyzed.Results CT scan showed 17 cases of artery-portal vein fistulae (centre),10 cases of artery-the small branch of portal vein fistulae(periphery),3 cases of artery-liver vein fistulae,5 cases combined the signs aforementioned,2 cases combined the artery-portal vein fistulae and the artery-liver vein fistulae,and the 13 cases no the characteristic signs of CT,37 cases of the signs of AVF were found by CT,sensitivity 74%.26 cases were diagnosed as AVF,postitive rate 52%.Conclusion The development of portal vein and/or the inferior caval vein ahead in artery phase,especially the parenchyma of the lobes,segments and subsegments of liver,may be a foundation of the diagnosis of AVF.And we discussed the limitation of the CT examination in diagnosing this complication.

The effects of Haimaichong Nutrient on the contents of Lipo peroxide(LPO),Lipofuscin(LPF)and the activities of the old rats were investigated.After Haimaichong(HMC) was given 60 day,the results showed that the contents of LPO in blood and liver were significantly decreased (P

Objective To study the status of mouse fetal transgenic cells existed in the maternal blood in peri-natal period and provide the information for gene diagnosis using fetal cells circulated in the maternal blood.Methods Mating the female normal mice with male transgenic mice integrated with GFP reporter gene driven by ?-globin promoter and HS2-HS3 elements of LCR. Around the offspring were born, maternal blood was collected and GFP expression level was determined with FACS analysis. Meanwhile, DNA extracted from the tails of the mothers and their offspring as well as the maternal blood were analyzed by PCR.Results GFP positive fetal cells were not found in maternal blood before offspring were born. 1-3 weeks later, GFP positive fetal cells were detected and this population in maternal peripheral blood reached the peak. 4-5 weeks later, they disappeared gradually. PCR results showed no GFP positive band in the mothers. However, a positive fragment of GFP gene was amplified in maternal blood samples after their offspring were born. This result was in accordance with FACS analysis.Conclusion Transgene cells may be useful markers for the study of status of fetal cells existed in the maternal circulating blood. The results would be beneficial for the gene diagnosis using maternal blood as an alternative resource of fetal cells.

Objective To evaluate the quality of Semen Cassiae from different habitats objectively. Methods To determine the content of chryso-phanic according to ChP and establish HPLC fingerprints with the gradient elution solvent composed of acetonitrile and 1% HAC. A C18 column (250 mm?4.6 mm, 5 ?m) was used, flow rate: 1 mL/min, detecting wavelength: 254 nm, and the column temperature: room temperature. The clustering analysis was carried out by SAS software according to the content of chrysophanic and similarity of HPLC fingerprints obtained by the software of similarity analysis. Results The established HPLC fingerprint has desirable precision, reproducibility, and stability. The content of chrysophanic and HPLC fingerprints of Semen Cassiae from various habitats are different, which differs from the habitats. The content range of chrysophanic in Semen Cassiae is 0.037%-0.170% and the similarity is 0.864-0.962. Conclusion The method indicates the difference of the chemical component in Semen Cassiae from various habitats and can be used as a quality control method for Semen Cassiae.

Objective: To study the protective effects of polypeptide-Fe on acute hepatic injury induced by CCl4 in mice. Method:Mice model of acute hepatic injury was set up by CCl4 ig (50mg/kg?d ). and fed with polypeptide-Fe 67.5 mg/kg?d, 675 mg/kg?d and 2 025 mg/kg?d for 30 d respectively. The effects on serum ALT, AST, and ALP and liver TG, TC, GSH, MDA, and SOD avtivities were observed. Results: Polypeptide-Fe decreased the activities of ALT, AST, and ALP in serum and the levels of TG, TC, and MDA in liver, and increased the level of GSH in liver. Middle dosage group of polypeptide-Fe was the most effective one. Conclusion:Polypeptide-Fe showed significant antioxidant ability in protecting acute hepatic injury induced by CCl4 in mice.

Objective To observe the effect of Rosuvastatin on the function of vascular endothelial and inflammatory response in patients with acute myocardial infarction ( AMI) after intervention. Methods Seventy-eight patients with AMI patients underwent coronary angiography and PCI treatment The patients were divided into the control group (38 patients) and the Rosuvastatin group (40 patients) after PCI treatment All patients were routinely used nitrates, low molecular weight heparin,aspirin,clopidogrel,the Rosuvastatin group were treated on the basis of conventional medication plus service Rosuvastatin 10 mg/time, once a day. Both groups were continuous medicated for 6 months. Cardiovascular events of all patients, the serum lipids, inflammation-related biomarkers and indicators of vascular endothelial function were followed up for 6 months after discharge. Results The cardiovascular events in the Rosuvastatin group (5. 0% ) was significantly lower than that of the control group (18.4%) after discharge within 6 months (χ2 = 4. 52, P < 0. 05). After 6 months of discharge, the serum lipids, the serum IL-6, hs-CRP,plasma ET-1 and ox-LDL of Rosuvastatin group ((2. 16 ±0. 54)mmol/L,(4. 16 ±0. 28)mmol/L,(6. 80 ± 2. 65) ng/L, (4. 02 ± 1. 58) mg/L, ( 62. 45 ± 9. 38) ng/L and 381. 65 ± 39. 73, respectively) was significantly lower than those of the control group ((2.47 ±0. 59) mmol/L, (5. 29 ±0. 31 )mmol/L, (9. 39 ±4. 17) ng/L, (5. 76 ± 1. 52)ng/L, (81. 75 ± 10. 23) ng/L and 485. 91 ±42. 68,respectively) (t =2. 423,16. 910,3. 291,4. 952,8. 691 and 11. 173 , respectively , P <0. 01 ). The serum NO of Rosuvastatin group ( ( 62. 17 ± 17. 69 ) μmol/L) was significantly higher than than that of the control group ( (48. 27 ±18. 35 ) μmol/L) (t = 3.406, P < 0.01 ) . The serious adverse events were not observed in the Rosuvastatin group. Conclusions Rosuvastatin reduces inflammatory reaction after PCI in patients with AMI, improves endothelial function, and reduces adverse reactions.

The antifatigued effect of Haimaichong on the rats has been studied.When the rats were given Haimaichong nutrient 15 days,the motion endurance,the activity of lactic dehydrogenase the removing speed of lactate and the contents of the glycogen in the liver and in the muscle have been enhanced markedly;The level of blood sugar during the long time of motion can be kept steadily;The product of urea nitrogen in the serum has been decreased notably in motion and removed swiftly after motion.The results suggest that Haimaichong nutrient have the antifatigued effect strikingly.

Vinflunine tartrate-loaded liposomes (VT-L) with two drug-to-lipid ratios were prepared by pH gradient method. Vesicle size and zeta potential were determined by the Zetasizer Nano ZS. Entrapment efficiency was evaluated by cation exchange resin centrifugalization method. The toxicity and tumor inhibition to nude mouse administrated by VT-L with different drug-to-lipid ratios were investigated and compared with the vinflunine tartrate injection (VT-I). The results showed that the mean particle size, zeta potential and entrapment efficiency of the VT-L with drug-to-lipid ratios of 1 : 5 and 1 : 10 were 124.6 nm and 128.3 nm, -25.3 mV and -22.8 mV, 94.46% and 97.31%, respectively. The VT-L with two different drug-to-lipid ratios has significantly higher anti-tumor effect to nude mouse transplanted human non-small cell lung carcinoma A549 and lower toxicity than VT-I. While there were no significant differences in anti-tumor effect and toxicity between VT-L with two different drug-to-lipid ratios.