Objective: A correction factor was proposed to improve the accuracy of dose calculation in conventional radiotherapy when filed matching technique was used. Methods:Use the linear accelerator with independent collimator to implement semi-field irradiation under different equivalent-length field. Measure the dose and compare it with the calculation dose. Obtain a correction factor which change follow the equivalent-length through data fitting. Results:There is relationship to indicate the correction factor increase if the field area enlarge;the calculation dose will be close to the measure dose, and the accuracy will improve by correcting;Conclusion:The clinical dose of semi-field irradiation can be calculated by an equivalent square field technique , and then multiplied by the correction factor. it is a simple method to improve the accuracy of dose calculation in conventional radiotherapy by using semi-filed correction factor when filed matching technique was used.

Objective To examine the effect of berberine on tumor necrosis factor-α(TNF-α)-induced VEGF expression and the possible mechanism.Methods There were two sessions in this study.In the first session,HepG2 cells were treated with 10 ng/mL TNF-αfor 0,12,24 and 48 h,respectively.In the second session,HepG2 cells were pre-incubated with 0,10,25 and 50μmol/L berberine for 24 h,respectively,and then treated with 10 ng/mL TNF-αfor 24 h.The effect of berberine on the prolif-eration of HepG2 cells was assayed by MTT.The level of VEGF mRNA was detected by real-time PCR,and the expression lev-els of VEGF,IκBαand NF-κB p65 proteins by Western blot.The activity of NF-κB was measured by ELISA.Results Berberine could dose-dependently inhibit the growth of HepG2 cells.TNF-αcould increase the mRNA and protein expression levels of VEGF in HepG2 cells in a time-dependent manner.Berberine could significantly dose-dependently down-regulate the TNF-α-in-duced VEGF expression at mRNA and protein levels.TNF-αsignificantly decreased the expression of IκBαand increased the ex-pression of NF-κB p65 and the activity of NF-κB in a time-dependent manner,which could be dose-dependently abrogated by pretreatment with berberine.Conclusion Berberine inhibits TNF-α-induced VEGF expression via NF-κB signaling pathway.

Objective To investigate the practical application values of copper sulfate method and hemocyte analyzer in blood do‐nation screening .Methods A total of 1 500 blood samples of donors were randomly collected and determined by using both copper sulfate method and hemocyte analyzer .Taking hemocyte analyzer as the reference method ,the sensitivity and specificity of copper sulfate method for detecting hemoglobin(Hb) were calculated .The overall detection rates of abnormal blood samples determinded by the two methods were calculated ,as well .Statistical comparisons were performed on the test data from both methods .Results A total of 29 donors(accounted for 1 .9% ) with unacceptable Hb value were found by using copper sulfate method .For all blood do‐nors ,the sensitivity and specificity of copper sulfate method for Hb screening before blood donation was 99 .7% and 82 .8% ,respec‐tively .And there were no statistically significant differences in sensitivity and specificity between the two methods for Hb estima‐tion(P>0 .05) .There were 336 donors with abnormal blood samples detected by using hemocyte analyzer .Taking hemocyte analy‐zer as the reference method ,only 8 .6% of donors with abnormal blood samples were screened out by using copper sulfate method . Conclusion The copper sulfate method could be used for Hb screening before blood donation .While because of its limitations ,such as relatively low specificity and inadequate screening items ,the hemocyte analyzer shoud be utilized before blood donation if the con‐ditions permit .

Objective:To design a machine for trauma patients to be weighed and translocation, increase the accuracy of clinical drug using, reducing secondary damage and improve the life quality.Methods: The machine which consists of mechanical system, driving system, weighing system, power supply system, control system. It has the advantages of high efficiency and safety, simple operation, easy translation motion.Results:It resolved the problems in weighing and translocation.Conclusion: The machine reduces the labor intensity of medical staff, and avoids the secondary damage in the transportation process at the same time. With its good effect, the machine obtains very high value in clinical application.

Objective To investigate the changes in kinetic parameters of the major joints of the lower extremity during walking in patients with knee osteoarthritis(KOA),and explore its mechanism for clinical treatment.Methods Sixty patients with KOA in tibia-femoral joint and 30 healthy persons as control group(CON) were recruited.Patients were divided into less severe KOA group(LKOA)and more severe KOA group(MKOA)in accordance to K/L radiological grade,then the kinetic data by 3D-gait analysis system were collect.Results LKOA had more knee maximal abduction movement than MKOA and CON after heel strike(P<0.001);the knee first peak adduction movement of MKOA was significantly greater than LKOA(P<0.05)and CON(P<0.001)during medial stance phase;the knee second peak adduction movement of LKOA was significantly less than CON(P<0.05);the hip first peak adduction movement of MKOA was significantly greater than CON(P<0.05).Conclusion The more severe level of KOA in tibia-femoral joint,the more load on medial compartment of patient's knees was seen,comparing with CON,patients with KOA have greater hip abduction movement and more severe patients have less hip adduction movement.

Objective To observe the factors affecting inadequate ST-segment resolution in patients with acute ST-segment elevation myocardial infarction after emergency percutaneous coronary intervention (PCI). Methods The patients with acute ST-segment elevation myocardial infarction undergoing emergency PCI were enrolled for study. According to the ratio of ST-segment resolution, 186 cases were divided into inadequate ST-segment resolution group (54 cases) and relatively adequate ST-segment resolution (132 cases). Clinical data of two groups were compared. The single factor and multiple Logistic regression analysis were performed to determine the factors influencing inadequate ST-segment resolution. Results Pre-infarction angina was protective factor of inadequate ST-segment resolution after emergency PCI (OR=0.361, 95%CI 0.131-0.994, P<0.05). Anterior myocardial infarction, attack-to-balloon time, Killip classification and white blood cell counts were risk factors of inadequate ST-segment resolution (OR=2.389, 95%CI 1.194-4.781;OR=1.655, 95% CI 1.082-2.532; OR=1.319, 95% CI 1.026-1.695; OR=1.184, 95% CI 1.004-1.396, P<0.05). Conclusions Patients with pre-infarction angina could reduce the risk of inadequate ST-segment resolution after PCI. Patients with anterior myocardial infarction,long attack-to-balloon time, Killip classification≥2 and high blood cell counts could increase the risk of inadequate ST-segment resolution, and earlier and more active clinical intervention should be taken.

Objective To study the signs and the value of CT enhanced scanning in diagnosing the liver cancer combined arteriovenous fistulae(AVF).Methods 50 cases of the liver cancer combined AVF had plain and enhanced scanning by spiral CT,then proved by hepatic arteriography.The signs of CT and hepatic arteriography were contrast analyzed.Results CT scan showed 17 cases of artery-portal vein fistulae (centre),10 cases of artery-the small branch of portal vein fistulae(periphery),3 cases of artery-liver vein fistulae,5 cases combined the signs aforementioned,2 cases combined the artery-portal vein fistulae and the artery-liver vein fistulae,and the 13 cases no the characteristic signs of CT,37 cases of the signs of AVF were found by CT,sensitivity 74%.26 cases were diagnosed as AVF,postitive rate 52%.Conclusion The development of portal vein and/or the inferior caval vein ahead in artery phase,especially the parenchyma of the lobes,segments and subsegments of liver,may be a foundation of the diagnosis of AVF.And we discussed the limitation of the CT examination in diagnosing this complication.

The effects of Haimaichong Nutrient on the contents of Lipo peroxide(LPO),Lipofuscin(LPF)and the activities of the old rats were investigated.After Haimaichong(HMC) was given 60 day,the results showed that the contents of LPO in blood and liver were significantly decreased (P

Objective To investigate the effect of partial sleep deprivation (PSD) on cardiac electric activity and function of vascular endothelium. Methods Eighteen Sprague-Dawley rats were randomly divided into two groups (9 each):PSD group and control group. PSD lasted 10 days,20 hours each day. Body weight and surface electrocardiogram (S-ECG) were examined and recorded 1d before PSD,and 1d,4d,7d and 10d after PSD. Serum concentrations of glucose,total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C),creatin kinase (CK),creatin kinase-MB (CK-MB),high-sensitivity C-reactive protein (hs-CRP),nitric oxide (NO),von Willebrand factor (vWf),endothelin-1 (ET-1) and E-selectin were determined the next day after 10d of PSD. Results Compared to that 1d before PSD,body weight in PSD groups decreased significantly,while increased obviously in control group. Compared to that in control group,the body weight of rats decreased significantly (P

The antifatigued effect of Haimaichong on the rats has been studied.When the rats were given Haimaichong nutrient 15 days,the motion endurance,the activity of lactic dehydrogenase the removing speed of lactate and the contents of the glycogen in the liver and in the muscle have been enhanced markedly;The level of blood sugar during the long time of motion can be kept steadily;The product of urea nitrogen in the serum has been decreased notably in motion and removed swiftly after motion.The results suggest that Haimaichong nutrient have the antifatigued effect strikingly.