Objective To establish mice modle of allergic rhinitis(AR) and to study the role of Staphylococcal enterotoxin B(SEB) and ovalbumin(OVA) in the modle.Methods Forty Balb/c mice were evenly randomized into OVA group,SEB group,OVA+SEB group and normal sodium group and AR modle was established.The symptom scores,total serum IgE concentration,IL-4 concentration were analyzed by factorial design.Meanwhile,the morphology change of nasal mucosa was observed.Results The symptom scores in OVA group,SEB group,OVA+SEB group and normal sodium group were 6.80?1.03,0.90?0.99,0.70?0.82,0.60?0.70 respectirely.The interaction of OVA and SEB had statistical significance(P

OBJECTIVE To establish mice model of allergic rhinitis(AR) and study the role of Staphylococcal enterotoxin B(SEB)and ovalbumin(OVA)in the model,and investigate the change of regulatory T cell(Treg)in the nasal mucosa of mice.METHODS Forty Balb/c mice were randomized into OVA group(A),SEB group(B),OVA+SEB group(C)and normal solution group(D).AR model was established.The symptom scores and count of Foxp3 positive cells in nasal mucosa were analyzed by factorial design.RESULTS The symptom scores in Group A,B,C,D were 0.90?0.99,0.70?0.82,6.80?1.03,0.60?0.70 respectively.Group C was successfully established as AR model.OVA and SEB had interaction(P

Objective To evaluate the application effect of clinical pathways (CP) in children with bronchopneumonia.Methods The literature of randomized controlled trials (RCTs) in children with bronchopneumonia were retrieved by searching PubMed,Cochrane Library,Web of Knowledge,Wanfang database,VIP,and CNKI from their inception date to March 2014.Meta-analysis was performed by Revman 5.2software.Results Six RCTs involving 24 559 patients were finally selected,with 12 269 cases in the CP group and 12 290 cases in the traditional treatment group.The results of Meta-analysis showed that there were significant differences between the CP group and the traditional treatment group in the cure rate [OR =1.67,95 ％ CI (1.03,2.77),P =0.04],length of hospital stay [MD =-1.21,95 ％ CI (-1.95,-0.48),P =0.001],hospitalization expenses [SMD =-3.68,95 ％ CI (-5.04,-2.32),P ＜ 0.000 1],days of antibiotic using [MD =-1.20,95 ％ CI (-1.51,-0.89),P ＜ 0.000 01] and patient satisfaction [OR =6.12,95 ％ CI(3.12,12.04),P ＜ 0.000 01].Conclusion CP is superior compared with the traditional treatment for bronchopneumonia,and is worthy of spread in clinical.

Objective To investigate the clinical value of serum homocysteine (Hcy),Cystatin C (CysC) detection in coronary heart disease prevention,diagnosis and treatment.Methods A total of 200 cases of coronary heart disease patients including 50 cases of acute myocardiac infarction (AMI) (AMI group),85 cases of unstable angina pectoris (UAP)(UAP group.) and 65 cases of stable angina pectoris (SAP) (SAP group) were selected,and 120 cases of healthy controls were selected as control group.The serum levels of Hcy and CysC were detected and compared.Results The serum levels of Hcy and CysC in AMI group,UAP group and SAP group were significandy higher than those in control group[Hcy:(22.35 ± 8.18),(16.54±7.56),(14.52±6.38) μmol/Lvs.(9.35±3.23) μmol/L; CysC:(1.32±0.27),(1.88± 0.66),(1.19 ± 0.46) mg/L vs.(0.80 ± 0.33) mg/L],and there were significant differences between two groups (P＜ 0.01).The serum level of Hcy in AMI group was higher than that in UAP group and SAP group [(22.35 ± 8.18) μ mol/L vs.(16.54 ± 7.56),(14.52 ± 6.38) μ mol/L],and there was significant difference (P ＜ 0.01).Conclusions The relationship between Hcy,CysC level and coronary heart disease is close,and Hcy levels increase with increasing degree of coronary heart disease.Detection of Hcy and CysC has some clinical value on prevention,diagnosis,treatment of coronary heart disease.

Objective To observe the cell proliferation inhibition of DNA methyltransferase (DNMT) inhibitors decitabine (DAC) combined with daunorubicin (DNR) in human leukemia cell line HL-60.Methods The effects of DNR and DAC were examined in HL-60 cells by cell viability using MTT method,and cell death using flow cytometric (FCM).Results DAC,DNR single drug application showed their effects on cell proliferation was dependent of dose and time,the inhibition effect of combined treatment group was much clearer [inhibition ratio of 72 hours was (80.23±1.71) ％,P ＜ 0.001].The highest apoptosis rate was at 5.0 μmol/L DAC combined with 1.0 μmol/L DNR for 72 hours,which was statistic significant (F =30.199,P ＜ 0.001).Combinations of different concentrations of DAC and DNR increased expression of PTEN mRNA in concentration-dependent manner,which was significantly higher than the control group and DNR single drug group (F =578.218,P ＜ 0.001).Conclusions DAC can significantly inhibit the proliferation of HL-60 cells and induce apoptosis,synergistic effect can be observed when DAC combined with DNR.The underlying mechanism can be due to DAC demethylation effect to increase PTEN mRNA expression.

OBJECTIVE:To observe therapeutic efficacy of zoledronic acid in the treatment of acute pain of vertebral compres-sion fractures during different courses. METHODS:207 patients with acute thoracic or (and) lumbar vertebral compression frac-tures were selected and randomly divided into trial group(107 cases)and control group(100 cases). Control group was given calci-um carbonate(1 200 mg/d)diclofenac sodium sustained-released tablet(75 mg/d).Trial group was additionally given zoledronic ac-id 5 mg added into 5%Glucose solution 100 ml,intravenous dripping for 15-30 min,on the basis of control group. RESULTS:Af-ter treatment,VAS score of two group decreased significantly,and trial group the decrease was more significant than control group,with statistical significance (P<0.05),there was no statistical significance in VAS score between course≤one month and one month 0.05);after 4d treatment,there was statistical significance in VAS score between course≤one month and one month 0.05);after one week,there was statistical significance in VAS score between one month 2 months (P<0.05).CONCLUSIONS:Zoledronic acid may partly alleviate the pain of acute vertebral fractures,the analgesic effect is more clear in early stage.

Aim To investigate the relationship between GABAA receptor or NMDA receptor and the amnestic effect induced by etomidate.Methods Amnestic model was established by intraperitoneal injection of etomidate(3 mg?kg-1) in mice before intracerebroventricular injection of different doses of bicuculline or NMDA,then the error times,step down latency and step through latency were observed and recorded in the step down test and step through test.Results Bicuculline(2,4 ?g) instead of NMDA by intracerebroventricular injection could decrease the error times and increase the step down latency and step through latency of amnestic mice in the step down test and step through test.Conclusion GABAA receptor rather than NMDA receptor may be an important target for the amnestic effect induced by etomidate.

Aim To observe the effects of ketamine on Na+,K+-ATPase,Ca2+-ATPase and NOSase activity in different cerebral cortex in convulsive mice.Methods The mice were randomly divided into blank group,normal saline(NS) group and ketamine 25 mg?kg-1 (KetⅠ),50 mg?kg-1(KetⅡ) group. The animals of blank group were killed directly.Convulsion was induced by intraperitoneally(ip) strychnine(1.5 mg?kg-1) in other groups,and correspond drugs were administered ip before five minutes.Action variety of mice was observed. Animals were killed on 30 minutes after strychnine injection.The activity of Na+,K+-ATPase,Ca2+-ATPase,TNOSase and iNOSase were assessed by spestrophotometric analysis in different cere-bral cortex(forehead,parietal and occipital area).Results Ketamine group could decrease mortality completely. The duration of tonic state in KetⅡ group was significantly shorter than that in KetⅠgroup.Compared with blank group,Na+,K+-ATPase and Ca2+-ATP ase activities were decreased in the group of NS and KetⅠ,and recovered normal level in the group of KetⅡ at parietal and occipital area. TNOS ase activity was decreased by 1/3 in KetⅡ group(P

Aim To investigate the relationship between amnestic effect of enflurane or isoflurane and NMDA receptor.Methods Amnestic model was established by intraperitoneal injection of enflurane(0.4 ml?kg-1)or isoflurane(0.3 ml?kg-1)respectively in mice before intracerebroventricular injection of different doses of NMDA(25,50,75 ng),then the error times,step down latency and step through latency were observed in the step down test and step through test.Results NMDA(50,75 ng)by intracerebroventricular injection could decrease the error times,and increase the step down latency and step through latency of amnestic mice induced by enflurane or isoflurane in the step down test and step through test.Conclusions NMDA by intracerebroventricular injection can improve amnestic effect of enflurane or isoflurane partially.NMDA receptor may be an important target for amnestic effect of enflurane or isoflurane.

Aim To investigate the relationship between amnestic effect of ketamine,propofol or sodium oxybate and NMDA receptor.Methods Amnestic model was established by intraperitoneal injection of ketamine (20 mg?kg~-1),propofol(10 mg?kg~-1) or sodium oxybate(100 mg?kg~-1) respectively in mice before intracerebroventricular injection of NMDA,and then the error times,step down latency and step through latency were observed in the step down test and step through test.Results NMDA by intracerebroventricular injection decreased the error times and increased the step down latency and step through latency of amnestic mice induced by ketamine.It had no significant impact on those of amnestic mice induced by propofol or sodium oxybate.Conclusion NMDA receptor may be an important target for amnestic effect of ketamine,rather than the target for amnestic effect of propofol or sodium oxybate.