Cross-Cultural Comparison ; Humans ; Medicine, Chinese Traditional ; Terminology as Topic ; Translations

Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Objective:To search for the efficient immune routes for rotavirus DNA vaccine by evaluating efficacy in inducing immune responses to rotavirus.Methods:In this study BALB/c mice were immunized by intramuscular and intranasal routes with rotavirus DNA vaccine,pcDNA 1/VP7,then rotavirus specific systemic immunity was measured.Results:Serum rotavirus specific IgG but not IgA titers of mice immunized by both routes were significantly higher than that of the unimmunized controls.Conclusion:Both intramuscular and intranasal routes are candidates for rotavirus DNA vaccine.

Objective To establish and verify a capillary isoelectric focusing-whole column imaging detection(CIEFWCID) method for the analysis of isoelectric point(pI) of recombinant human brain natriuretic peptide.Methods The ampholyte,space-occupying agent,protein concentration,focusing time were optimized by CIEF-WCID method,and the best condition for the detection of recombinant human brain natriuretic peptide was obtained.The repeatability,precision and durability of the developed method were verified,and three batches of recombinant human brain natriuretic peptide produced continuously were analyzed for pI.Results HR AESlyte 8-10.5 was selected as ampholyte,while 25 mmol/L sodium hydroxide as the space-occupying agent;The final concentration of the sample was 87.5 μg/mL and the focusing time was 8min.The relative standard deviation RSD of pI detection was 0.1% after six consecutive injections of the same sample;The RSD of pI detection of six samples was 0.1%;The pI RSD of the main peak was 0.1% at different final concentrations of the sample,and the pI RSD of the sample was 0.1% at different storage time,while the pI markers could not be changed arbitrarily.The pI was detected in three consecutive batches of recombinant human brain natriuretic peptide samples.Conclusion The developed CIEF-WCID method for pI analysis of recombinant human brain natriuretic peptide had good repeatability and precision and might be used for follow-up quality control of recombinant human brain natriuretic peptide.

ObjectiveTo observe the effect and safety of neurotropin on essential trigeminal neuralgia in aged patients.MethodsThe numeric rating scales (NRS) of the aged patients was used to evaluate before and after treatment.ResultsThe score of NRS decreased significantly after neuiotropin injection.ConclusionNeurotropin injection may be an effective and safe treatment on essential trigeminal neuralgia.

Objective To investigate the clinical use of low molecular weight heparin (LMWH) with thromboelastography (TEG)R time monitoring in malignant tumor patients, and guide clinical rational drug use. Methods 145 patients with malignant tumors were respectively first times and second times with low molecular weight heparin 4 h after venous blood determination of TEG R time and anti Xa activity, compare the effects of different doses of LMWH on TEG R time; the effects of different doses of LMWH against the activity of Xa; the correlation between the doses of LMWH at first time and TEG R time; the correlation between the doses of LMWH at first time and the activity of anti Xa; the correlation between TEG R time and the doses of LMWH at first time. Results Different doses of LMWH treatment had a significant effect on the TER G time (P < 0.05); no significant effect of different doses of LMWH against the activity of Xa; TEG R time with the first LMWH dose significantly correlated (P < 0.05); anti Xa activity and LMWH at the first time had no significant correlation between the dosage; no significant correlation with the anti Xa activity of TEG R time. Conclusion Using TEG R time to detect the clinical application of low molecular weight heparin is more sensitive and accurate compared with anti Xa activity detecting in patients with malignant tumor.

Objective To explore the effects of resveratrol on the express of inflammatory factors (IL‐1 ,IL‐6 ,TNF‐α)of hu‐man umbilical vein endothelial cells(HUVEC) induced by Aβ1 -42 .Methods HUVEC were stimulated with Aβ1 -42 5 × 101 μmol/L , last 24 h and administrated with resveratrol 160 ,80 ,40 ,20 μg/L ,HUVEC viability were detected by CCK‐8 ;the concentration of IL‐1 ,IL‐6 and TNF‐α were detected by ELISA .Results Compared with model group ,160 ,80 ,40 ,20 μg/L resveratrol could in‐crease HUVEC survival rate ,reduced HUVEC damage by Aβ1 -42 ,inhibited the concentration of IL‐1 ,IL‐6 and TNF‐α(P<0 .05) . Conclusion Resveratrol over 20 μg/L could reduce the release of IL‐1 ,IL‐6 and TNF‐αof HUVEC inducing by Aβ1 -42 .

Objective To explore the effect of applying PDCA Circulation Theory in practical teaching of Basic Nursing at Higher Vocational College.Methods By using cluster random sampling method 2 classes of each 50 people from the nursing class of Grade 2013 were selected as the control group and the experimental group respectively.The traditional method of practice teaching was used in control group,that is,from the presentation of teachers,students practice,the guidance of teachers,periodic assessment to the final examination,while the PDCA Circulation Theory was used in experimental group.With periodic skill examination result,the final skill examination result and students' learning experience as evaluation index,two groups were compared with t test,using SPSS 17.0 for data analysis,expressed as percentage and-x ± s.Results The results of both single skill examination and the final skill examination as well as study experience of experimental group were higher than those of control group,and the differences are statistically significant.Conclusion Using PDCA Circulation Theory in practical teaching of Basic Nursing at Higher Vocational College can stimulate the students' consciousness and initiative,cultivate students' critical thinking,promote the student to study in a planned way with purpose and system and improve the learning effect.

Objective To investigate the clinical effect of Allevyn Thin on phlebitis caused by PICC. Methods Total 60 patients with phlebitis caused by PICC were randomly divided into 2 groups:experiment group and control group. The patients in the experiment group were treated with Allevyn Thin, while the patients in the control group were treated with hydropathic compress of 50% magnesium sulfate solution. The curative effect and healing time of the two methods were observed and compared. Results The average healing time of the experiment group was significantly shorter than that of the control group, and the curative effect of the experiment group was significantly better than that of the control group (P<0.05). Conclusion Allevyn Thin can shorten the healing time and improve the curative rate in treating phlebitis caused by PICC, suggesting that Allevyn Thin has a good potential of extensive clinical application.