Objective:To study the relationship of stressful life events,coping styles and emotional disorder.Methods:345 junior middle school students were collected as sample.They were tested with Stressful Life Event Questionnaire,Coping Style Questionnaire,SAS and SDS.Results:The coping style played an important role on the anxiety and depression;and stressful life events influenced the anxiety and depression indirectly through the coping styles.Conclusion:Anxiety and depression were determined by the emotional-focused coping style.

Papillary thyroid cancer (PTC) is the most common type in thyroid carcinomas.Recently,the prevalence and diagnostic rate of PTC has got significantly high with the general use of ultrasound.Nowadays,more and more studies have suggested that the coexistence of PTC and diabetes is common.They indicated that hyperglycemia would induce the deterioration of oxidative stress injury,chronic inflammation,insulin resistance,obesity,dyslipidemia,deficiency of vitamin D and dysimmunity.All of these may break the balance of oxidation and antioxidation and result in disordering signal pathway,accumulation of inflammatory cytokines,over activating of insulin and insulin-like growth factor-1,changing the metabolic pathways,which will promote the occurrence and progression of PTC.

Adult ; Cord Blood Stem Cell Transplantation ; adverse effects ; Cytomegalovirus ; Cytomegalovirus Infections ; complications ; Female ; Hemolysis ; Humans

OBJECTIVEThis study aimed to investigate the duration of gingival healing after the stage II surgery of dental implantation for periodontitis patients and to provide clinical guidelines for implant restoration.

METHODSTwenty-nine periodontitis patients who had implantation surgery and achieved osseointegration were operated with stage II surgery (a total of 60 pieces of implants). The height of buccal gingival of each implant was measured twice after the stage II surgery. All implants were measured at the lowest point ofbuccal gingival after one week. The implants were randomly divided into four groups according to the schedule of the next test time: group one at one week from the initial test point, group two at two weeks, group three at three weeks, and group four at four weeks. Each group includes 15 pieces of implants. The amount of the buccal gingival change in each group between the second and first tests was determined, and the data were analyzed statistically.

RESULTSThe amount of gingival change of groups one, two, three, and four was (-0.25 +/- 0.66), (-0.04 +/- 0.52), (-0.70 +/- 0.77), and (-0.74 +/- 1.09) mm, respectively. No significant difference was observed between groups one and two in terms of the amount of gingival changes (P > 0.05). However, a significant difference was found between groups two and three (P < 0.05), and the amount of gingival recession was 0.66 mm. No significant difference was found between groups three and four (P > 0.05), and the gingival achieved stability.

CONCLUSIONThe gingival recession achieves stability at the fourth week (after 28 d) after stage II surgery. At this time, the implant can be restored, and the abutment can be selected according to the amount of gingival change of the periodontitis patient.

Alveolar Bone Loss ; Dental Implantation ; Dental Implantation, Endosseous ; Dental Implants ; Dental Restoration Failure ; Gingiva ; Gingival Recession ; Humans ; Osseointegration ; Periodontitis

Objective To establish a method to quantify the residual cross-linker vinyl sulfone and its hydration product 1 ,4-Thioxane-1 ,1-Dioxide.Methods The polysaccharide was precipitated by ethanol,however vinyl sulfone and its hydration product were soluble,and can be analyzed by direct injection.The analysis was carried on Agilent DB-wax capillary column (30m ×0.53 mm,1.0 μm).The flame ionization detector (FID)and flame photometric detector (FPD)were used to detect samples and the efficiency were compared. Results The linear range of vinyl sulfone and its hydration product were separately 0.5 ~20μg/mL and 2~100μg/mL detected by FID.The Limit of Quantity (LOQ)were 0.8μg/mL and 2.3μg/mL, respctively.The Limit of Detection (LOD)were 2.6μg/mLand 7.6μg/mL,respectively,and the average recoveries (n=9)of them were 104.3%and 92.4%,respectively.Conclusion FID could meet the needs of the test,and this method is simple and accurate with high sensitivity and good repeatability,which can be used for quality control of trace vinyl sulfone and its hydration products 1 ,4-Thioxane-1 ,1-Dioxide in cross-linked sodium hyaluronate injection.

Background Keratectasia after laser in situ keratomileusis (LASIK) is one of the serious complications which affect the vision.The measurement of cornea posterior elevation is an important way helping to find this change.Objective The aim of this study was to investigate the change of cornea posterior elevation after LASIK in myopic eyes with astigmatism and affecting factors.Methods A series case-observational study was adopted.One hundred and twenty-seven myopic eyes with astigmatism of 66 patients who received LASIK in 2008 May through 2010 January in Beijing Tongren Hospital were retrospectively analyzed to evaluate the change of cornea posterior elevation following the LASIK.The parameters related to cornea posterior elevation were measured and compared before and 3 months,6 months and 1 year after surgery,respectively,with Oculyzer anterial segment analysis system.Results The height values from posterior cornea highest point,the lowest point and central vertex central elevation zone were (12.20±3.39),(-19.02±7.38) and (1.05 ±3.25) μm respectively before LASIK and were (14.38±3.80),(-18.55±7.11),(2.83±4.81)μm in3 months and (13.99±3.38),(-17.57±6.54),(2.45±4.61) μm in 6 months after LASIK.They were (14.40±3.85),(-17.76±6.00),(2.16±5.00) μm in 1 year after surgery.Significant increases were found in the vertex height and central elevation after LASIK compared with before surgery(highest pointq=6.813,5.594,6.875,all at P＜0.001.central vertex:q=4.488,P=0.002;q =3.530,P=0.013 ;q =2.799,P =0.047).However,no significant difference was seen in various time points after LASIK (P＞0.05).A positive correlation was obtained between the height value of the posterior cornea central vertex with the spherical equivalent (SE),maximum cutting depth or cutting proportion 1 year after LASIK (r =0.295,0.297,0.295,all at P=0.001),and there was a negative correlation between it with residual stroma (r=-0.208,P=0.019).The intraocular pressure (IOP) measured with noncontact tonometry (NCT) was (14.24±3.33) mmHg before LASIK,and those of 3 months,6 months and 1 year were (8.42± 1.90),(8.61 ± 1.64) and (8.76± 1.64)mmHg after LASIK,showing a significant lowing in IOP after LASIK in comparison with before LASIK (q =29.851,28.317,26.337,all at P＜0.001).But no significant change was found in the different time points after LASIK (P＞0.05).There was significant difference in the IOP corrected by Ehlers after LASIK (P＞0.05).The IOP before LASIK had positive correlation with the central elevation of posterior cornea surface 1 year after LASIK(r=0.258,P =0.003).Conclusions The cornea posterior elevation increases slightly early stage after LASIK but retains stable level with lapse of time.The refraction diopter before LASIK is the main factor affecting the posterior corneal shape.To reduce the risk of corneal ectasia,more attention should be paid to the reserving of appropriate corneal residual stroma and smallest degree of eccentricity during the ablation procedure.

Indocyanine green (ICG) is a fluorescent dye which has various advantages including high absorption,low toxicity,not involved in biotransformation in vivo and rapid excretion in near-infrared range.Due to the high penetrating ability in biological tissues and low rate of being affected by other factors,near-infrared optical imaging has become a major method of functional imaging and diagnosis.It shows great prospects in optical detection.As the only clinical using near-infrared optical imaging contrast enhancer approved by FDA,ICG has been widely used in biomedical imaging,photodynamic therapy,pathology detection and many other medical related areas.This article focuses on the recent development of ICG application,especially concentrates on the clinical uses.

Bacteriophage is a kind of virus depending on bacterium,named bacterial virus,and it can multiply in bacterium.There're six types of Chlamydiophage discovered which are Chp1,Chp2,Chp3,Chp4,CPAR39 and PhiCPG1.Capsid proteins Vp1,Vp2 and Vp3 are three major structural proteins of Chlamydiophage.The study of Chlamydiophage will play great action on chlamydia infection therapy.

Objective To explore the value of the combined detection of helper T lymphocyte (Th)1 and 2 type cytokines ex‐pression in the evaluation of acute pancreatitis severity and treatment efficacy .Methods From May 2009 to June 2013 ,the enzyme‐linked immunosorbent assay (ELISA ) was adopted to detect the expression levels of Th1‐type cytokineinterferon‐γ (IFN‐γ) and Th2‐type cytokine interleukin (IL)‐4 in 32 cases of mild acute pancreatitis (MAP group) and 18 cases of severe acute pancreatitis (SAP group) on 1 ,4 ,7 d after admission as well as 15 cases of normal healthy people (control group) .Results The test results of IFN‐γ on 1 ,4 ,7 d after admission in the MAP group were (31 .47 ± 4 .35) ,(36 .92 ± 4 .12) ,(49 .09 ± 4 .97)ng/L ,which of IL‐4 were (54 .72 ± 5 .52) ,(45 .26 ± 4 .89) ,(39 .79 ± 3 .85)ng/L .The test results of IFN‐γ on 1 ,4 ,7 d after admission in the SAP group were (23 .97 ± 5 .39) ,(26 .29 ± 3 .47) ,(33 .64 ± 4 .47)ng/L ,and which of IL‐4 were (70 .45 ± 5 .05) ,(65 .99 ± 4 .58) ,(56 .23 ± 4 .23)ng/L .The test results of IFN‐γ and IL‐4 in the normal control group were (49 .26 ± 20 .67)ng/L and (35 .15 ± 16 .32)ng /L respectively .Compared with the normal control group ,the expressions of Th1‐type cytokine IFN‐γ in the MAP group (except which on 7 d) and peripheral blood Th1‐type cytokine IFN‐γ in the SAP group were decreased and the expression of Th2‐type cyto‐kine IL‐4 was increased ,the difference was statistically significant (P< 0 .05) ;The test results of each index in the same treatment time had statistically significant differences between the SAP group and the MAP group (P< 0 .05) .Conclusion The obvious po‐larization of Th1‐type cytokine and Th2‐type cytokine exists in acute pancreatitis ,and the polarization degree is related with the se‐verity of disease .The combined detection of Th1‐type cytokine and Th2‐type cytokine is beneficial to judge the extent of serious condition and treatment effect of acute pancreatitis .

Objective To investigate the clinical significance of the glucose screening tests during pregnancy. Methods 1 516 cases of 24～28 weeks pregnant women were given 50g glucose screening tests, and for those whose blood glucose ≥7. 8 mmol/L further underwent glucose tolerance tests(OGTT) for a clear diagnosis. Results The incidence of gestational diabetes is 3.17% and the incidence of impaired glucose tolerance in pregnancy is 2. 64%. Conclusion 50g glucose screening for pregnant women helps to carry out early diagnosis and treatment of gestational diabetes,to improve the quality of obstetric and reduce perinatal mortality rate,it is of great significance.