Nausea and vomiting are among the most common complications after surgery. Postoperative nausea and vomiting(PONV) may cause wound dehiscence, pulmonary aspiration, dehydration, and electrolyte disturbances. Despite the new classes of antiemetics and research published, PONV remains high incidence still. This article covers the different pharmacologic strategies taken introduced with multimodal approach by using antiemetic drugs for prevention and treatment of PONV.

Objective To understand the side-effects of cyclophosphamide (CTX) in the treatment of systemic autoimmune diseases and the possible risk factors. Methods Two hundred and forty-one patients with systemic autoimmune diseases were recruited from the Rheumatology Division of Peking University First Hospital during January 1st, 2009 and March 31, 2012. All the patients received oral or intravenous cyclopho-sphamide. The data were collected by medical record review as well as telephone follow-up. Logistic regression analyses were used for statistical analysis. Results Statistical analysis for age, sex, disease, cumulative dose, treatment duration and mode of administration were included in the factor analysis that would impact the CTX related side-effects. Age ( x2=14.8, P=0.002), gender ( x2=11.2, P=0.001), the underlying disease ( x2=26.1, P<0.01), cumulative dose ( x2=9.8, P=0.007) and mode of administration of CTX ( x2=19.5, P<0.01) were all correlated with the incidence of CTX side-effects. Multivariate analysis showed that women [OR=2.32, 95%CI (1.15, 4.70), P=0.02], intravenous-oral sequential use of CTX [OR=5.25, 95%CI (2.30, 11.97), P<0.01] and systemic lupus erythematosus [OR=4.02, 95%CI (2.24, 7.21), P<0.01] as the underlying disease were independent risk factors for CTX side-effects. Conclusion Alopecia, gastrointestinal discomfort and gonads toxicity ware com-monly seen in Chinese patients with systemic autoimmune diseases receiving CTX. Hemor-rhagic cystitis is very rare. Women, intravenous-oral sequential use of CTX and the systemic lupus erythematosus are indepen-dent risk factors for CTX side-effects.

The application status and disadvantages of lecture-based learning ( LBL ) and problem-based learning (PBL) have been compared and discussed, and the imagination of combina-tion of LBL with PBL in the application of biotechnological pharmaceutics teaching proposed. LBL teaching will be mainly applied in the fundamental principles of development and production of biotechnological pharmaceutics, while PBL teaching will be mainly applied in the specific production processes of typical biopharmaceutics. The three key points in the combination teaching process includ-ing the time allocation of LBL and PBL, the design and implementation of PBL cases and the founda-tion of reasonable teaching evaluation system have also been introduced. The combination of LBL with PBL will cultivate students' self-study, comprehensive analysis and teamwork capabilities to satisfy the demands of the development of current market for biopharmaceutical talents.

Autophagy ; Liver Cirrhosis ; physiopathology

OBJECTIVETo explore the value of the application of oil red O staining in spinal cord injury (SCI) of rats.

METHODSWith simple randomization, 24 Spargue-Dawley male rats were divided into normal control group including 6, and SCI group including 18. Spinal cord was transected at spinal lever T10 to build SCI model. Six rats of SCI group were sacrificed randomly at 1, 2, 4 weeks after surgery. After the spinal cord tissue sections were made, oil red O staining methods were used to observe the changes at the end of transected spinal cord. Images were analyzed by Image-Pro Plus 6.0 and SPSS 20.0 software.

RESULTSThe oil red O staining of normal control group showed that white matter surrounded by myelin sheath was clear and obviously distinctive from grey matter. Uneven and strengthened staining in oil O was observed in grey matter of SCI group at 1, 2, 4 weeks post-SCI.

CONCLUSIONIt is a good method to label the myelin sheath in spinal cord and distinct white matter from grey matter by oil red O staining. Analysis of the images showed that lipid may become another target for drugs, which needs more researches.

Animals ; Azo Compounds ; Gray Matter ; pathology ; Male ; Nerve Fibers ; physiology ; Nerve Regeneration ; Rats ; Rats, Sprague-Dawley ; Spinal Cord Injuries ; pathology ; Staining and Labeling

OBJECTIVETo observe the efficacy difference on the motor function in the children of cerebral palsy between acupuncture combined with acupoint embedding therapy and simple acupuncture.

METHODSOne hundred and twenty children of cerebral palsy were randomized into an observation group and a control group, 60 cases in, each one. In the observation group, acupuncture was applied in combination with acupoint embedding therapy. For the acupoint embedding therapy, the main acupoints were Pishu (BL 20), Shenshu (BL 23); the supplementary points were Dazhui (GV 14), Mingmen (GV 4), Yaoyangguan (GV 3), Sanjiaoshu (BL 22), Xinshu (BL 15) and Ganshu (BL 18). The catgut was embedded to the acupoints. Acupuncture was applied to the acupoints on the head and four limbs and the needles were retained for 40 min after qi arrival. In the control group, the simple acupuncture was applied, except for the acupoint embedding therapy. The rest treatment was the same as the observation group. The treatment lasted continuously for 3 months in the two groups. Before and after treatment, the gross motor function measure (GMFM) and the gross motor function classification system (GMFCS) were used for the assessment and the efficacy was analyzed.

RESULTS(1) After treatment, the total score of GMFM was increased in the two groups (both P<0. 01) and the score in the observation group was higher than that in the control group (P<0. 05). (2) The scores in A and B areas of GMFM were all improved in the two groups after treat ment (all P<0. 01) and the scores in the observation group were higher than those in the control group (P<0. 01 P<0. 05). (3) The total effective rate was 76. 7% (46/60) in the observation group, which was better than 55. 0% (33/60) in the control group (P<0. 01).

CONCLUSIONThe combined therapy of acupuncture and acupoint embedding much better improves the motor function in the children of cerebral palsy as compared with the simple acupuncture.

Acupuncture Points ; Acupuncture Therapy ; Catgut ; utilization ; Cerebral Palsy ; physiopathology ; therapy ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Motor Activity

Objective Valsartan, the angiotensin Ⅱ type 1 receptor blocker, is recently proved to reduce urinary albumin at the microalbuminuria stage in human diabetic nephropathy without altering glucose metabolism. But the pathway is still uncertain. In present study, we examined the changes of adrenomedullin receptor (ADMR) mRNA and protein expressions in the renal cortex of diabetic rats to investigate the protective effects of valsartan on an experimental model of diabetic renal injury. Method The SD rats were randomly divided into following groups: normal rats, STZ-induced diabetic rats, and diabetic rats treated with valsartan. STZ-induced diabetic rats were treated with valsartan (10mg/kg body weight) or vehicle for 8 weeks. The expressions of ADMR mRNA in renal cortex were analyzed by RT-PCR, as well as ADMR protein expressions were detected through western blot. Results We found (1) Valsartan treatments reduced urinary albumin excretion in 24h, compared with the untreated. But no notable difference was seen in HbA1c and blood sugar of diabetic rats between the two groups. (2) Valsartan treatments increased the expressions of ADMR mRNA and protein in diabetic rats renal cortex. Conclusion These results indicate that valsartan treatment can upgrade the expressions of ADMR in the renal cortex of diabetic rats. It may be one of renal protective pathways of Ang Ⅱ type 1 receptor blocker.

Objective To study material basis of Zhou's prescription, components of effective parts Ⅰ in Zhou's prescription was gualitative and quantitative analyzed. Methods The components of effective part Ⅰ was identified by test-tube reaction and qualitative TLC method, the content of saponins and flavones was quantitative analyzed. Results Total content of the saponins and flavone are 1.07% and 0.85% in Zhou's Prescription. The purity of saponins and flavones in effective parts Ⅰ are 37.4% and 29.7%. Conclusion The main components of effective part Ⅰ in Zhou's Prescription were flavones and saponins.

ABRO1 (Abraxas Brother 1), known also as KIAA0157 or FAM175B, is an important component of BRISC deubiquitinating enzyme ( DUB) complex which specifically shears K-63 linked polyubiquitin chains .This article reviews the research progress in ABRO 1 in terms of its structural characteristics , BRISC complex composition , the regulation of DUB activity and interferon response , oxidative stress response and anti-myocardial ischemia and provides new ideas on fur-ther study of the physiological function of ABRO 1 and its association with immune and cardiovascular diseases .

Objective To explore the clinical manifestation and laboratory examination of chronic diarrhea in children with acquired immunodeficiency syndrome(AIDS) and analyze the reasons that causes the chronic diarrhea in children.Methods Clinical data of 7 patients (male 4 cases,famale 3 cases)with AIDS with manifestation of chronic diarrhea among the 17 cases were diagnosed as AIDS in Beijing children′s hospital from Jan.1999 to Dec.2006.The time of diarrhea,degree of dinrrhea,characteristic of stool,accompaniment symptom,laboratory examination were retrospectively analyzed.Results The average onset age was 6 years old(from 2-9 years old).The average time of diarrhea was 6 months(from 1-16 months).Four cases main complained with chronic diarrhea,3 cases came to the hospital because of fever,cough and wasting;6 cases with diarrhea,7 cases with malnutrition and anemia,5 cases accompanied hypokalemia and metabolic acidosis,6 cases had delay in growth and development,2 cases with abnormal stool routine exam,1 case with positive stool culture of fungi.All patients were with lower CD4,5 cases with lower CD4/CD8.Conclusions Chronic diarrhea is a common symptom in children with AIDS,and usually accompanied by obvious delay in growth and development,malnutrition and anemia,the reasons that causes the chronic diarrhea are consi-dered to be related with both the opportunistic infection and HIV infection.