Objective To observe the effect of Da Jianzhong Decoction on cerebral cyclooxygenase-2 (COX-2) mRNA and protein and calmodulin-dependent protein kinase II(CaMKⅡ) mRNA in spleen-yang deficiency pain rats, so as to investigate the therapeutic mechanism of Da Jianzhong Decoction for spleen-yang deficiency pain. Methods Forty SD rats were randomly divided into normal group, model group, and high-and low-dose Da Jianzhong Decoction groups(3, 0.75 g·kg-1·d-1), 10 in each group. Spleen deficiency rat model was established by comprehensive modeling methods. After the rats in various groups were given gastric administration of Chinese medicine for 15 continuous days, rat cervical dislocation was performed for the sampling of the brain tissue. The expression levels of COX-2 mRNA and CaMKⅡmRNA in brain tissue were detected by real time polymerase chain reaction (RT-PCR). The COX-2 protein expression was determined by immunohistochemistry. Results Compared with the normal group, mRNA and protein expression levels of COX-2 in rat brain tissue of spleen-yang deficiency model group were increased(P﹤0.01), and CaMKⅡ mRNA expression was decreased (P<0.01). Compared with the model group, COX-2 mRNA and protein expression levels were decreased(P<0.01) and CaMKⅡexpression was increased(P<0.01) in high-and low-dose Da Jianzhong Decoction groups(P<0.01). Conclusion Da Jianzhong Decoction can suppress cerebral COX-2 mRNA and protein expression, and up-regulate CaMKⅡmRNA expression, which probably contribute to its therapeutic mechanism for relieving the pain.

Objective To investigate the changes of inflammatory factors in mice infected with Vibrio alginolyticus .Methods Established a V .alginolyticus ATCC17749 T‐infected mouse model through intraperitoneal injection ,then performed the median le‐thal dose experiment ,the detection of hematological and liver function indicators ,histopathologic evaluation and inflammatory cyto‐kine detection .Results The median lethal dose of V .alginolyticus ATCC17749T was 1 × 109 CFU by intraperitoneal injection .The dose of 1 × 109 CFU per mouse was adopted in the following study .WBC and platelet numbers significantly decreased after infection (P<0 .05) ,RBC number and hemoglobin content significantly increased(P<0 .05) and ALT ,AST significantly increased after V . alginolyticus infection(P<0 .05);light microscopy observation revealed that ,hepatic cell and pulmonary tissue suffered serious inju‐ry after infection.Through the detection of antibody array ,the levels of 20 inflammatory factors were found changed significantly . 8 of them including KC ,IL‐6 ,RNATES ,IL‐12 ,Eotaxin ,G‐CSF ,MIP‐1a ,Mig ,the expression of which increased more than 10 times .Conclusion The inflammatory factors analysed in the paper provide a theoretical basis for the study of the inflammatory mechanism of Vibrio alginolyticus infection .

World Health Organization aims to develop safe, effective and practical traditional medicine. Traditional Chinese medicine (TCM) and other complementary and alternative medicine are being recognized in the whole world nowadays. However, the definite effect of Chinese medicine is still in need of scientific research proof. Placebo control is of equal importance to active control and blank control in clinical trial of TCM. This article briefly reviewed the importance of placebo control and commented on its present situation in clinical trial of TCM. This article also brought up the preliminary proposals of placebo application in TCM clinical trial. We should emphasize scientific placebo preparation and good design of placebo-controlled trial, which are directed by International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. A good clinical trial project will avoid unnecessary wastes and provide safe and effective treatment for people.

To investigate the application of the teaching model of disease-based PBL combined with the interest group in clinical ophthalmology teaching. A clinical ophthalmology interest group was set up among interns and a PBL teaching with the theme of a single disease was carried out. The students demonstrated group presentation and discussion, and finally the teacher made comments in combination with clinical experience. The results shows that students' feedback was good. It has mobilized students' enthusiasm and initiative and can be used as a transition for carrying out PBL teaching complement to the traditional teaching model and be applied to the clinical ophthalmology teaching.

Objective To investigate the effects of 2-(3carboxy-1-oxoprogy1)amino-2-deoxy-D-glucose(COPADG) on the proliferation and cell cycle of human esophageal cancer cells Eca-109 and TE-1.Methods In vitro experiments,MTT colorimetric assay was performed to determine the growth inhibitory rates of Eca-109 and TE-1 cells treated by COPADG.Flow cytometry was applied to observe the change of cell proliferation cycle and apoptosis index.Cell morphological change was observed by transmission electron microscopy.Results COPADG inhibited effectively the growth of esophageal cancer cells Eca-109 and TE-1 in a time-and dose-dependent manner(P

This article discusses the variation process of the sense of justice in western and traditional Chinese society,and its effect on regulatory ethics.Western regulatory ethics takes "making use of evil to restrain evil" as its regulatory principle,whose purpose is "to increase goodness by evil",while Chinese regulatory ethics employs "taking goodness in restraining evil" as its regulatory principle.The Chinese regulatory ethics puts morality in the first place,using morality to replace regulatory systems.The distinctions of cultures and the sense of justice between western and Chinese societies cause the different viewpoints in relevant systems and regulations.

Objective To express M cell-targeted fusion peptide SAM-GFP in prokaryotic cells, purify it and identify its targeting properties, so as to lay a foundation for the research of M cell-targeted oral vaccine against Helicobacter pylori.Methods The GFP gene was synthesized by PCR-based accurate synthesis(PAS) and cloned into the vector pCzn1-SAM to construct the recombinant plasmid pCzn1-SAM-GFP. The recombinant plasmid was transformed into competent E.coli Arctic Express, induced by IPTG, and then purified by Ni-NTA affinity chromatography. The obtained fusion protein SAM-GFP was identified by 12% SDS-PAGE, Western blot and fluorescence microscopy. The M-cell targeting properties of SAM-GFP fusion protein was verified by immunofluorescence staining using mouse ileal loop model.Results The recombinant plasmid pCzn1-SAM-GFP was constructed correctly as identified by double digestion and sequencing. The fusion protein SAM-GFP had a relative molecular mass of about 54 900, and was mainly expressed in soluble form with a purity of 94. 5% after purification. It showed specific binding to anti-6 × His/GFP monoclonal antibody, and green fluorescence was observed under fluorescence microscope. SAM-GFP could be specifically uptaken by small intestinal M cells. Conclusion The fusion protein SAMGFP with M-cell targeted function was successfully expressed and purified, which lays a foundation for the subsequent research and development of oral vaccine against Helicobacter pylori.

Heritable changes in gene expression are regarded as epigenetics,which do not involve coding sequence modifications.The study of ophthalmology epigenetics is a rapidly growing area in biomedical research.Epigenetic mechanisms principally include DNA methylation,histone modification,chromatin remodeling and noncoding RNA.Aberrant DNA methylation and histone modification are linked to a number of age-related disorders,such as cancer,autoimmune and others.In recent years,the modulations of epigenetic changes on the pathogenesis of eye disorders and their roles in therapeutic interventions are drawing more and more attention,and these studies deepen the understanding of relevant diseases.Since the epigenetic alterations are reversible,modifying epigenetic marks contributing to eye diseases provide a new approach to the development of disease prevention,diagnosis and therapies.Herein we discuss the roles of epigenetic changes in eye disease development,hoping that ophthalmologists and researchers pay attention to these researching cues in pathogenesis of eye disorders caused by genetic expression alterations in response to environmental changes,importantly,to the implication for relevant eye disease therapy and prevention.

Objective To investigate the effect of spironolactone combined with valsartan on renal function in patients with early diabetic nephropathy.Methods 158 early diabetic nephropathy patients from May 2014 to June 2015 in department of endocrinology of Nanxun People’s Hospital of Huzhou were selected and divided into control group (79 cases) and experimental group (79 cases).The control group were treated by valsartan and the experimental group were treated with spironolactone on the basis of control group.The changes of renal function, potassium ion and blood rheology were observed and compared after treatment.Results Compared with control group post-treatment, the systolic pressure and diastolic pressure levels were lower, plasma urinary albumin excretion rates (UAER) level was lower (P <0.05), albumin (Alb), serum creatinine (Scr), blood urea nitrogen ( BUN) , creatinine clearance ( Ccr) and K +levels had no significant differences, whole blood specific viscosity, plasma specific viscosity and whole blood specific reduced viscosity were lower ( P <0.05 ) in experimental group.Conclusion Spironolactone combined with valsartan could effectively improve clinical efficacy of patients with early diabetic nephropathy , improve the renal function of patients, recover ion disorder and reduce blood viscosity.

Objective To investigate the effect of Guizhi Fuling pills on levels of serum MAPK, ERK and VEGF in patients with endometriosis. Methods 60 patients with endometriosis were selected and according to the random control table were divided into the experiment group and the control group, 30 cases each.The control group were treated with conventional acetate goserelin sustained-release implantation agent subcutaneous injection, the experiment group were treated by Guizhi Fuling pill treatment.After treatment, the clinical efficacy, serum MAPK, ERK-5, VEGF, ERK, T-cad and VE-cad levels of the patients were compared.Results Compared with the control group, the clinical total effective rate was higher in the experiment group (P<0.05);MAPK and ERK-5 levels in serum were lower (P<0.05), ERK level in serum was lower(P<0.05);T-cad and VE-cad levels in serum were lower(P<0.05);VEGF level in serum was lower(P<0.05).Conclusion Guizhi Fuling pill can block the cell signal transduction pathway through inhibition of MEK and ERK activity, thereby inhibit abnormal endometrial proliferation and cell division cycle, can effectively inhibit the proliferation and differentiation of the tumor.