Objective:To explore the isolation,culture,proliferation and fluor-labelling of the fetal bone mesenchymal stem cell (BMSCs),and their biological character. Methods:Limb bone marrow samples from fetus aged 4 to 5 months were obtained, BMSCs were isolated by Ficoll centrifugalization and adherence screening method,and then cultured,proliferated to the 12th generation in vitro. The growth curve,cell cycle,phenotype,enzymatic activity,glycogen and the DAPI fluor-labelling of BMSCs were observed. Results:(1)Major BMSCs were fusiform, arranged as whirlpool. After the 12th generation,BMSCs presented the signs of senility. The subcultured cells proliferated faster than primary cells. (2)The 3rd and 8th generation of BMSCs expressed CD29,D71,but not CD34,HLA-DR;the G0,G1 period cells were 93.73%,and the S,M,G2 period cells were 6.27%. (3)The 5th generation cells were positive of nonspecific esterase and glycogen,but weak positive of alkaline phosphatase. (4)DAPI labeled BMSCs grew normaly,and the marking rate of BMSCs was 100%,but decreased gradually,and only 15% cells were labbelled for the 3th week. Conclusion:The acquired fetal BMSCs have the character of MSCs. The DAPI labeling is a modus operandi for the short period tracing BMSCs.

Objective To establish HPLC method for determination of the related substances in gatifloxacin mesylate for injection.Methods Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).All the chief peaks of gatifloxacin and those of their related substances were well isolated in all the specificity tests.the detective limit was 0.038?g/ml(S/N=4).the total peak area of therelated substances of all batches of samples was not more 1% of the peak area of their solutions.Conclusions This method is simple,accurate and stable.

Objective To establish the method for the determination of gatifloxacin mesylate for injection.Methods The chromatographic condition was as follows:Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0 ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).At the concentration of 80% 、100%、120%,the mean recovery were 99.45%(RSD 1.29%,n=3)、99.24%(RSD 0.47%,n=3)、99.81%(RSD 0.75%,n=3).,respectively.Conclusion The method is accurate with a good reproducibility and can be used as a quantitative analys for preparation.

Animals ; Humans ; Phytotherapy ; Rheumatic Diseases ; drug therapy ; Stilbenes ; therapeutic use

BACKGROUND: BK viral infection after renal transplantation influences the prognosis of BK virus-associated nephropathy in renal transplant recipients. The disease has been widely studied in foreign countries.OBJECTIVE: This study was designed to sum up the diagnosis and treatment of BK virus-associated nephropathy after renal transplantation.RETRIVAL STRATEGY: Using the terms "renal transplantation; BK virus" in the English language, manuscripts responsible for BK virus-associated nephropathy after renal transplantation that were published from January 2000 to January 2007 were retrieved from the PubMed database. A total of 206 manuscripts were obtained and primarily screened. Inclusion criteria: studies addressing BK virus-associated nephropathy after renal transplantation. Exclusion criteria: repetitive studies.LITERATURE EVALUATION: The included manuscripts were primarily from PubMed database. Manuscripts were primarily original and review studies.DATA SYNTHESIS: BK virus can be found in the urine of 3%-40% of adult renal transplant recipients. BK virus reactivation rate is very high, but the histological manifestations of BK virus associated nephropathy are found only in a small number of renal transplant recipients. The prognosis of BK virus associated nephropathy is very poor. BK virus associated nephropathy develops into renal failure, leading to transplant loss, in 30%-50% patients. BK virus-caused renal transplant disease must be diagnosed according to histological manifestations. Viral infection should be primarily confirmed, but serological measurements have no predominant effects. Electron microscopy should be involved in the assessment of renal graft biopsy, especially when renal failure factors are unknown, as through the use of electron microscope, viral particles in the nucleus, cytoplasm and outside of the cells could be detectable. Viral antigen in the urine sample of patients with BK viruria can be detected by nucleic acid hybridization method, immunofluorescence, and ELISA. Cidofovir is an effective medicine for treatment of BK virus-associated nephropathy, but its potential nephrotoxicity, proper dose, pharmacokinetics, safety, tolerance, anti-BK viral activities have not been confirmed. So fresher study should be required for aforementioned uncertainties.CONCLUSION: BK virus-associated nephropathy has poor prognosis and should be diagnosed according to histological examinations. BK virus-associated nephropathy has been treated primarily by symptomatic supportive treatment and reducing the dose of immunosuppressive agents.

Objective To explore the preliminary practice of Clinical Scenario Mode in the teaching of surgery. Methods Clinical Scenario Mode was stimulated or rebuilt by the cooperation of teachers and Standardized Patients (SP) to introduce relative theories and raise discussions in the surgery lectures of five-year clinical medicine students in grade 2004 and grade 2005. Results Lectures with the application of Clinical Scenario Mode were reported positively by students from the survey. It improved students’learning motivation,enhanced teaching quality,and optimized the allocation of resources. Discussion With the combination of the advantages of lecture based learning (LBL) and problem based learning (PBL),Clinical Scenario Mode can be considered as the linkage to the widespread use of PBL,and should be widely applied in the medical teaching practice.

Objective To study the analgesic effect of sufentanil combined with Dezocine in endoscopic resection of fallopian.MethodsA total of 188 patients with tubal pregnancy from April 2013 to December 2014 in Haining central hospital were enrolled in the study, they were randomly divided into two groups (n=94).On the basis of conventional intravenous analgesia, the patients in control group was were received sufentan, the observation group were given sufentan combined with dezocine.The score of pain at different time points after operation, the Ramsay sedation score, and the level of IL-6 and IL-10 were compared.The adverse reactions were compared between the two groups.ResultsThe score of VAS in the observation group was significantly lower than that in the control at 4h, 8h, 12h and 24h after operation (P<0.05).The score of VAS in the two groups were similar at 48h after operation;the Ramsay score in the observation group was significantly higher than that in the control group(P<0.05) at five time points (4h, 8h, 12h, 24h and 48h after operation);when compared Compared with the indexes before operation, the level of IL-6 was significantly increased in the two groups at 24h and 48h, but the observation group increased less, the IL-6 in observation group was lower than that in the control group at the two points(P<0.05);while the IL-10 was higher than that beforeoperation, and the observation group was more higher(P<0.05).The adverse reactions in the two groups were mainly dizziness, nausea, pruritus and lethargy, and the incidence was only 12.8% in the observation group, which lower than that in the control of 29.8%(P<0.05).ConclusionIt which dazocine combined with sufentanil had a good analgesic effect in the process of analgesia in gynecological laparoscopic resection of fallopian tube after the operation.The VAS score and Ramsay sedation score was improved significantly.The levels of inflammatory factors were lower, and the incidence of adverse reaction was significantly reduced.It is a reliable method for clinical intravenous analgesia.

Objective: A reversed phase HPLC method was described for determination of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules. Methods:The sample was separated on ODS column with mobile phase of methanol 40 mmol?L -1 sodium acetate pH 6.9 (35∶65) for prim O glucosylcimifugin and H 2O methanol THF(62∶38∶1) for 4′ O ? glucosyl 5 O methylvisamminol. The flow rate was 0.8 mL?min -1 , and the detection was set at 254 nm. Results: The calibration curves were linear in the range of 0.72 ?g?mL -1 ～6.5?g?mL -1 for prim O glucosylcimifugin and 0.92?g?mL -1 ～16.5?g?mL -1 for 4′ O ? D glucosyl 5 O methylvisamminol( r =0.9999). The average recovery was 100.3% and 94.7%. The content of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules was 0.133 mg?g -1 and 0.167 mg?g -1 , respectively. Conclusion: The method is fast and specific for both constitutents of Ganmaoqingre Granules.

10.3969/j.issn.2095-4344.2013.23.025

BACKGROUND:Diagnosis of pelvic and acetabular fractures is difficult with a high misdiagnosis rate. It is necessary to diagnose pelvic and acetabular fractures rapidly and accurately. And patients suffer great injury, blood loss, from traditional open pelvis and acetabulum surgery, often complicated by neurovascular injury. Correct positioning and placement of internal fixator can reduce surgical complications.
OBJECTIVE:To explore the new progress of three-dimensional reconstruction of pelvic and acetabular fractures in order to promote its clinical application.
METHODS:A computer-based online search of PubMed database, CNKI database and Vip database between September 1996 and October 2013 was performed to search related articles using the key words of“3D, three dimensional reconstruction, pelvic fracture, acetabular fracture”in English and Chinese, respectively. The duplicate research or Meta analysis articles were eliminated. Final y, 41 articles were enrol ed for further analysis.
RESULTS AND CONCLUSION:Through a three-dimensional reconstruction using a variety of post-processing techniques, such as multi-planar reformation, maximum intensity projection, shaded surface display and volume rendering, we can get high quality images at any angle. Various post-processing techniques interact with each other that can reduce missed diagnosis rate and misdiagnosis of pelvic and acetabular fractures. Moreover, three-dimensional reconstruction techniques can be used to design surgical approach, guide surgeon’s surgical program designed to simulate surgical procedures, reduce the complications caused by the dislocation of internal fixation, and reduce the surgical risk. Three-dimensional reconstruction is also used for postoperative fol ow-up review of fracture recovery.