Objective:To explore the isolation,culture,proliferation and fluor-labelling of the fetal bone mesenchymal stem cell (BMSCs),and their biological character. Methods:Limb bone marrow samples from fetus aged 4 to 5 months were obtained, BMSCs were isolated by Ficoll centrifugalization and adherence screening method,and then cultured,proliferated to the 12th generation in vitro. The growth curve,cell cycle,phenotype,enzymatic activity,glycogen and the DAPI fluor-labelling of BMSCs were observed. Results:(1)Major BMSCs were fusiform, arranged as whirlpool. After the 12th generation,BMSCs presented the signs of senility. The subcultured cells proliferated faster than primary cells. (2)The 3rd and 8th generation of BMSCs expressed CD29,D71,but not CD34,HLA-DR;the G0,G1 period cells were 93.73%,and the S,M,G2 period cells were 6.27%. (3)The 5th generation cells were positive of nonspecific esterase and glycogen,but weak positive of alkaline phosphatase. (4)DAPI labeled BMSCs grew normaly,and the marking rate of BMSCs was 100%,but decreased gradually,and only 15% cells were labbelled for the 3th week. Conclusion:The acquired fetal BMSCs have the character of MSCs. The DAPI labeling is a modus operandi for the short period tracing BMSCs.

Objective To establish HPLC method for determination of the related substances in gatifloxacin mesylate for injection.Methods Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).All the chief peaks of gatifloxacin and those of their related substances were well isolated in all the specificity tests.the detective limit was 0.038?g/ml(S/N=4).the total peak area of therelated substances of all batches of samples was not more 1% of the peak area of their solutions.Conclusions This method is simple,accurate and stable.

Objective To establish the method for the determination of gatifloxacin mesylate for injection.Methods The chromatographic condition was as follows:Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0 ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).At the concentration of 80% 、100%、120%,the mean recovery were 99.45%(RSD 1.29%,n=3)、99.24%(RSD 0.47%,n=3)、99.81%(RSD 0.75%,n=3).,respectively.Conclusion The method is accurate with a good reproducibility and can be used as a quantitative analys for preparation.

Animals ; Humans ; Phytotherapy ; Rheumatic Diseases ; drug therapy ; Stilbenes ; therapeutic use

In order to further promote friendly cooperation with developing countries, Chinese government has been committed to increasing the intensity of cooperation and human resource development among developing countries. Healthcare is one of the key areas of China’s foreign aid. This article described the general conditions and experiences of national foreign aid training projects for developing countries undertaken by the Beijing Traditional Chinese Medicine Hospital and Beijing Traditional Chinese Medicine Research Institute. From aspects of participant enrollment, training program implementation, and training feedback, the training mode of national foreign aid on traditional medicine was introduced. This article focused on the selection of teachers, preparation of professional textbooks, and evaluation of teaching quality. Training programs over the years were guided by clear training concepts and accurate goal settings, which provided a useful exploration for the personnel training mode of national foreign aid on traditional medicine.

BACKGROUND:Sclerostin can negatively regulate the bone metabolism, and the sclerostin monoclonal
antibody can antagonize the negative regulation effect, inhibit bone resorption and promote bone formation. OBJECTIVE:To explore the mechanism and application progress of sclerostin monoclonal antibody in the treatment of osteoporosis.
METHODS:An online search of PubMed database, CNKI database, VIP database and Wanfang database
between May 2005 and May 2013 was performed by the first author to search the related articles with the key words of“osteoporosis, antibody, sclerostin, Wnt, SOST”in both English and Chinese. Articles related to
sclerostin monoclonal antibody were included. For the articles in the same field, those published earlier or in the authorized journals were preferred.
RESULTS AND CONCLUSION:A total of 170 articles were obtained after initial search, and final y 54 articles related to sclerostin monoclonal antibody were included for review according to the inclusion criteria. The
sclerostin can block Wnt pathway through combining with low-density lipoprotein receptor-related protein 5/6, thus inhibiting the differentiation and mineralization of osteoblasts. By specifical y binding to sclerostin, the
sclerosin monoclonal antibody can indirectly promote bone formation and restrain bone absorption which has great significance in the treatment of osteoporosis. Meanwhile, compared with the other treatment method, the specific targeting of sclerostin and the binding specificity of sclerostin monoclonal antibody provide application advantages for the treatment of osteoporosis.

10.3969/j.issn.2095-4344.2013.23.025

BACKGROUND:Diagnosis of pelvic and acetabular fractures is difficult with a high misdiagnosis rate. It is necessary to diagnose pelvic and acetabular fractures rapidly and accurately. And patients suffer great injury, blood loss, from traditional open pelvis and acetabulum surgery, often complicated by neurovascular injury. Correct positioning and placement of internal fixator can reduce surgical complications.
OBJECTIVE:To explore the new progress of three-dimensional reconstruction of pelvic and acetabular fractures in order to promote its clinical application.
METHODS:A computer-based online search of PubMed database, CNKI database and Vip database between September 1996 and October 2013 was performed to search related articles using the key words of“3D, three dimensional reconstruction, pelvic fracture, acetabular fracture”in English and Chinese, respectively. The duplicate research or Meta analysis articles were eliminated. Final y, 41 articles were enrol ed for further analysis.
RESULTS AND CONCLUSION:Through a three-dimensional reconstruction using a variety of post-processing techniques, such as multi-planar reformation, maximum intensity projection, shaded surface display and volume rendering, we can get high quality images at any angle. Various post-processing techniques interact with each other that can reduce missed diagnosis rate and misdiagnosis of pelvic and acetabular fractures. Moreover, three-dimensional reconstruction techniques can be used to design surgical approach, guide surgeon’s surgical program designed to simulate surgical procedures, reduce the complications caused by the dislocation of internal fixation, and reduce the surgical risk. Three-dimensional reconstruction is also used for postoperative fol ow-up review of fracture recovery.

OBJECTIVE:To strengthen the pharmacovigilance in hospital and to minimum the risk of drug in medical practice. METHODS: Literature optimization method and case analysis were applied for statemental analysis. RESULTS: Pharmacovigilance capacity of medical staff and propaganda and information exchange of pharmacovigilance should be strengthened. So the safety of clinical drug use could be improved. CONCLUSION: The leading role of hospital in pharmacovigilance should be full played to guarantee the safety of drug use.

Objective: A reversed phase HPLC method was described for determination of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules. Methods:The sample was separated on ODS column with mobile phase of methanol 40 mmol?L -1 sodium acetate pH 6.9 (35∶65) for prim O glucosylcimifugin and H 2O methanol THF(62∶38∶1) for 4′ O ? glucosyl 5 O methylvisamminol. The flow rate was 0.8 mL?min -1 , and the detection was set at 254 nm. Results: The calibration curves were linear in the range of 0.72 ?g?mL -1 ～6.5?g?mL -1 for prim O glucosylcimifugin and 0.92?g?mL -1 ～16.5?g?mL -1 for 4′ O ? D glucosyl 5 O methylvisamminol( r =0.9999). The average recovery was 100.3% and 94.7%. The content of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules was 0.133 mg?g -1 and 0.167 mg?g -1 , respectively. Conclusion: The method is fast and specific for both constitutents of Ganmaoqingre Granules.