OBJECTIVE To discuss the occupational hazard factors and protection measures of Central Supply Unit.METHODS The occupational hazard factors in the Central Supply Unit were analyzed and some suggestion on the occupational protections to decrease occupational risk was done.RESULTS It is necessary to give some special training about the occupational safety,reinforce the precaution of occupational hazard with regard to biology,physics,chemistry and motor function to establish some protection and countermeasures.CONCLUSIONS The pertinent occupational protection strategy according to the actual hazard factors is more efficient and more benefit for high quality management of Central Supply Unit which will enhance the standardization,quality and working efficiency,and ensure the work safety and quality.

Objective To study the expression and clinical significance of proliferating cell nuclear antigen(PCNA) and PTEN in endometrial carcinoma.Methods The expression of PTEN and PCNA of 18 cases of proliferative endometrium(normal group),21 cases of atypical hyperplasia of endometrium(precancerous lesion group),and 22 cases of endometrial carcinoma(endometrial carcinoma group) was determined immunohistochemically(SP method).Results PCNA expression in the normal group(16.1%),precancerous lesion group(71.4%),and endometrial carcinoma group(95.5%) was significantly different(P

Periodical assessment over the actual situation of physician work in a Class One Grade A hospital in Beijing is performed, and some ideas to improve the work are proposed in view of the problems existing in the work.

Objective To investigate the nalaxone treating first acute right heart failure.Methods52 patients with FARHF were divided into the naloxone group(30cases)and control group(20cases)at random.Patients in control group were given general comprehensively treating.Patients in naloxone group were given naloxone as well as comprehensively treating,were given intravenously 0.8 mg of first dose,if it was necessary,repeated 0.8 mg of dose,and continuously dropped for 1.2 mg was added into liquid 500 mL.After treatment of 24 hours,were compared the changes of HR、SBP、SV and heart function.ResultsIn the naloxone group,the total effective rates were 84.4%,the mortality rates were 6.6%;in control group.The total effective rates were 60%,the mortality rates were 18.3%.The difference of the effective rate of the naloxone group be treatment was statistically significant(P

Objective To study the effect and safety of controlled-release dinoprostone on the induced labor of late pregnancy . Methods 60 cases of late pregnancy puerpera were treated in our hospital from February 2014 to September 2015, and divided into the control group and the experimental group with reference to the stochastic indicator method, 30 cases in each group. The control group were given induced labor by oxytocin, and the experimental group were given induced labor by controlled-release dinoprostone, Changes of Bishop score, effects of promoting cervical mature, induced labor situation, and adverse reaction were compared between two groups. Results After medication, the cervical Bishop score of the group (8.35±0.93) points were significantly higher than (6.64±0.72) of the control group (P<0.05). The total effective rate 96.67％(29/30) of promoting cervical mature in the experimental group was significantly higher than 80.00％ (24/30) of the control group (P<0.05). Medicine to clinical time (14.89±1.57)h and total labor time (6.94±1.52) h of the experimental group were significantly better than (21.48±2.35) h and (9.30±1.31) h of the control group (P<0.05). The rate of delivery by vagina of the experimental group 83.33％ (25/30) was significantly higher than 60.00％ (18/30) of the control group (P<0.05). There was no different on adverse reaction rate between two groups. Conclusion There was good effects of Controlled-release dinoprostone on the induced labor of later pregnancy, with high rate of delivery by vagina and better safety.

Antigens, Differentiation, B-Lymphocyte ; genetics ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; genetics ; metabolism ; Gene Fusion ; Gene Rearrangement ; Histocompatibility Antigens Class II ; genetics ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Lung Neoplasms ; drug therapy ; genetics ; metabolism ; Protein Kinase Inhibitors ; therapeutic use ; Protein-Tyrosine Kinases ; antagonists & inhibitors ; genetics ; metabolism ; Proto-Oncogene Proteins ; antagonists & inhibitors ; genetics ; metabolism ; Pyrazoles ; therapeutic use ; Pyridines ; therapeutic use ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Sodium-Phosphate Cotransporter Proteins, Type IIb ; genetics

Objective To apply fluorescence-activated cell sorting (FACS) and magnetic activated cell sorting (MACS) in sorting natural killer (NK) cells from human peripheral blood. The efficiency of these two methods were compared so that the basic conditions for the further study on the lower proportion of peripheral blood cells could be created. Methods Twenty cases of normal peripheral blood and 3 cases of post-hematopoietic stem cell transplantation (post-HSCT) were collected and NK cell were sorted by FACS and MACS. Results Before sorting the normal peripheral blood, the proportion of the NK cell in the leukocyte was (12.86±3.62) %, the purity of NK cell was up to (96.15±2.03) % and the recovery was (95.08±2.16) %after sorting by FACS, the purity was up to (93.35±3.61) % and the recovery was (94.11±3.01) % by MACS.And before sorting the post-HSCT peripheral blood, the proportion of the NK cell in the leukocyte was (11.01±2.08) %, the purity of NK cell was up to (96.22±2.16) % and the recovery was (95.27±1.18) % after sorting by FACS. The purity was up to (90.98±1.94) % and the recovery was (94.54±3.52) % by MACS. Thereis difference in purity of NK cell (t = 5.925, P ＜0.05), while no difference in rate of recovery between 2methods (t = 0.789, P ＞0.05). Conclusion FACS is more efficient, rapidly and easily than MACS in sorting NK cell, especially for post-HSCT cases.

Objective To explore the effect of cucumber to moisten lips to alleviate xerostomia in patients with gastrointestinal decompression. Methods 180 patients were consecutively recruited at the department of gastric & intestinal surgery of the affiliated hospital of Guilin medical univemity from August 2010 to December 2011,they were randomly divided into the observation group and the control group,80 in each group.The observation group was given cucumber to moisten their lips besides conventional care,and the control group was only given conventional care.Finally we compared the two groups,and display difference between them in comfort degree of oral cavity. Results The comfort degree and oral cavity conditions of the observation group were better than those of the control group. Conclusions Giving cucumber to moisten lips can alleviate xerostomia and improve comfort level in patients with gastrointestinal decompression.

Objective To evaluate the clinical efficacy and toxicity of rituximab combined with CHOP (R-CHOP) in the treatment of relapsed or refractory diffuse large B cell lymphoma (DLBCL).Methods 30 patients were enrolled.All patients,pathologically confirmed to be CD20 positive DLBCL (all in stages Ⅲ-Ⅳ ), were relapsed or refractory after received 2-6 cycles of chemotherapy. Then all of them received R-CHOP schedule for 4 to 6 cycles, each cycle was 21 days. Clinical data before and after R-CHOP were collected.A retrospective analysis of the R-CHOP therapy,either compared to the literature or self-control was performed to evaluate the efficacy and toxicity. Results All the 30 patients were evaluable, induding 15 cases were complete remission (CR), 10 cases were partial remission (PR), 3 cases were stable disease (SD),and 2 cases were progressed disease (PD).The CR rate was 50.0 ％ (15/30),the total response rate (RR) was 83.3 ％ (25/30).All patients were well tolerated to the therapy.Only 3 cases were Ⅱ degree neutropenia,1 case was Ⅰ degree thrombocytopenia, and 2 cases suffered nausea and other mild gastrointestinal discomfort. Conclusions R-CHOP regimen could also achieve good response for relapsed or refractory DLBCL significantly.The common adverse effects of rituximab were mild.All the patients were well tolerated.

[Summary] Epigenetics defines heritable changes in gene expression that are not coded in the DNA sequence itself.Three systems,including DNA methylation,RNA-associated silencing and histone modification,are used to initiate and sustain epigenetic silencing.Type 2 diabetes mellitus(T2DM) is a polygenic disorder,which is caused by both genetic and environmental factors.However,pathogenesis of T2DM has not been elucidated.Recent studies have indicated that epigenetics may play an important role in the pathogenesis of T2DM,which is involved in the development and differentiation of β cell,secretion of β cell,insulin sensitivity and so on.Researches related to epigenetics may provide a new measure and target to clarify the pathogenesis of T2DM as well as prevent and treat the disease.