Neoadjuvant chemotherapy is one of the common means in multidisciplinary treatment of locally advanced breast cancer,with anthracyclines and taxanes as main chemotherapeutic drugs.In recent years,molecular target agents have significantly improved the efficacy of chemotherapy,as well as have provided more choices and ideas for clinical treatment.Currently,the widely studied molecular targeted drugs include trastuzumab,lapatinib,pertuzumab,T-DM1 and bevacizumab.

The function of cellular membrane receptor has been paid more attention in many dieases.Integrin is one of them.The major function of integrin is maintaining the proper structure of tissues by organization of cell-cell and cell-extracelluar matrix tethering.The initiation and prolongation of many diseases are promoted by these receptors.Disintegrin is one important component of the snake venom.It inhibit the adhesive attraction of integrin particularly.This review summarizes the types and the biological activities of snake venom disintegrins,as well as discusses there potential clinical application of the study,such as anti-tumor,anticoagulation,and various human diseases.

The syndrome of insulin-dependent diabetes mellitus(IDDM)includes not only abnor-mal glucose metabolism but also specific microvascular complications that include nephropathy,retinopathy and neuropathy.In the short term,improvement in the quality of life and possible prevention of further morbidity associated with diabetes makes pancreas trans-plantation an important therapeutic option,particularly when combined with a kidney trans-plant,in appropriately selected diabetic patients.The focus of the early management of the pancreas transplant recipient is the prevention of acute rejection.In this article we review the methods for clinical diagnosis of the early acute rejection due to pancreas transplanta-tion.

AIM:To separate and purify a new anti-clotting protein component(ACPF-7221)from the venom of Agkistrodon. The acute toxicity experiment of ACPF-7221 and the death rate were studied and the LD50 and 95% confidence interval were determined in mice. METHODS: The venom of Agkistrodon was separated and purified using ion-exchange chromatography of DEAE-Sepharose Fast Flow, molecular sieve filtration through Sephadex G75, SP-Sepharose Fast Flow and molecular sieve filtration through Sephadex G50, the anticoagulation components were recalled using activated partial thromboplastin time (APTT) activity assay. ACPF-7221 was given to mice by intravenous injection,and the dosage was determined according to the body weight of mice. The LD50 and 95% confidence were calculated by the death outcome. RESULTS: ACPF-7221 was separated and purified,which was a trimer consisted of three kinds of protein and their relative molecular mass were 25000, 30000 and 50000,and the protein level was 65%. The LD50 of ACPF-7221 was 7.848 mg/kg, and its 95% confident interval was 7.353-8.375 mg/kg. CONCLUSION: A new anti-clotting protein component ACPF-7221 was extracted from the venom of Agkistrodon by ion-exchange chromatography,which is a trimer consisted of three kinds of protein. The critical orga of acute toxicity is lung, empsyxis asphyxia deduces death.

Objective To investigate the impact of psoriacis on quality of life in patients with psoriasis. Methods A questionnaire interview included daily life and activities social activities and psychologic status were conducted of. Results 48 patients, 14 patients(29%) were impacted on quatity of life in heavy, 23 patiemts(48%) in middle, 11 patients(23%) in mildly no impact in zero. Conclusion The psoriasis valgaris influenced the Quality of life.

Objective To explore the influence of ROI size on the diagnostic performance of shear wave elstography (SWE) of breast masses.Methods A total of 225 consecutive patients with 239 solid breast masses underwent SWE before surgery,and then quantitative SWE parameters,including the maximum stiffness (Emax),mean stiffness (Emean),ratio of stiffness of the mass to stiffness of surrounding fat (Eratio) and standard deviation (SD),were measured by two methods,one method was using larger ROI to include the largest part of a mass,and the other was placing fixed ROI=2 mm over the stiffest part of the lesion to measure.The diagnostic performance of the two methods were evaluated by ROC curve,and the cut-off value,sensitivity and specificity of each SWE parameter were obtained.Results There were no statistically significant differences between the performance of Emax,Emean and SD from the two different methods (all P＞ 0.05),the performance of Eratio of the two methods showed significant difference (P=0.046).The significantly higher specificity of Emax and Emean in the larger ROI were found,and the significantly higher sensitivity of SD and Eratio in the ROI=2 mm was found.Furthermore,the diagnostic performance of combination of SWE and BI-RADS were not greatly affected by the ROI size,the difference had no statistical significance (all P＞0.05).Conclusion The ROI size only affects the diagnostic performance of Eratio,and donot affect the diagnostic performance of the quantitative SWE parameters (Emax,Emean,SD) and combination of SWE and BI-RADS.

Snake venoms are complex mixtures containing many different biologically active applications in the field of cancer research. In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin. The review mainly introduced the inhibitory effects of snake venoms on tumer metastasis.

BACKGROUND:With the consumption of acidic beverages, dental erosion becomes more serious. Erosion is related to direct loss of dental hard tissue, and causes dental over-abrasion, thus great threatening the dental health.
OBJECTIVE:To review the research work on the erosion of human teeth, erosion prevention, and teeth remineralization.
METHODS:A computer-based online retrieval of CNKI, Elsevier and Wiley databases between 1990 and 2013 was performed for articles abut human teeth erosion, erosion prevention and teeth remineralization. After repeated or old literatures were excluded, 58 literatures were included in the analysis.
RESULTS AND CONCLUSION:Current studies on dental erosion included three aspects, firstly, clinical observation of the symptoms and cause of dental erosion;secondly, the erosiveness of common acidic agents;and thirdly, the prevention of dental erosion. Many researchers focused on the prevention of dental erosion. It was suggested that dental erosion could be prevented through adjusting and control ing oral environment, enhancing the erosiveness of acidic beverages, and increasing the anti-erosion capacity of teeth. It should be noted that tooth erosion and friction/wear often occur simultaneously in the mouth. Therefore, future research should pay attention to the interaction mechanism of erosion, friction and wear of human teeth.

Tumor multidrug resistance (MDR) is the leading cause of clinical chemotherapy failure and death.Researches show that the occurrence of MDR is related to P-glycoprotein,multidrug resistance-associated proteins,breast cancer resistance-associated proteins,lung resistance-related proteins and other factors.Now the reversal strategies of MDR include chemical drugs reversal,gene reversal,immune reversal,traditional Chinese medicine reversal and drug-loaded nano-system reversal,which make some progress and help to improve the effect of cancer chemotherapy.

Objective To study the influence of resolving phlegm and relaxing bowels decoction(Huatan Tongfu decoction)on plasma tissue type plasminogen activator(t-PA),plasminogen activator inhibitor(PAI)and homocysteine(Hcy)in patients with acute cerebral infarction. Methods With randomized and controlled clinical research,78 inpatients from 2010 to 2012 in Traditional Chinese Medicine(TCM)Department of Beijing Chaoyang Hospital,Capital Medical University were chosen,including 40 patients in observation group and 38 patients in control group. The patients in control and observation groups were treated by conventional treatment,and additionally the patients in observation group received Huatan Tongfu decoction(ingredients:Trichosanthis 30 g,Arisaema with bile 6 g,Magnolia bark 10 g,Fried rice sprout 15 g),and the rhubarb decoction was made alone,each 20 mL decoction containing crude drug 6 g. 1-4 days after the beginning of the study,the two decoctions were mixed,and the patients took the lukewarm mixture orally twice daily(once 1/2 the dosage);5-12 days after the start of the study, the patients took 200 mL lukewarm mixed decoction daily,being divided into 2 times to administer,and according to the patient defecation situation,the dosage of rhubarb decoction was adjusted individually,the therapeutic course being 12 days. The t-PA,PAI and Hcy were detected before treatment and on the 7th day after treatment. TCM syndrome scores(phlegm syndrome,fire-heat syndrome and sthenic-fu syndrome)were recorded before and on the 12th day after treatment,and the scores of National Institute of Health Stroke Scale(NIHSS)were recorded at the same time. Results Before treatment,the differences in t-PA,PAI,Hcy levels and NIHSS score and phlegm syndrome,fire-heat syndrome and sthenic-fu syndrome scores were not statistically significant;all the indicators improved significantly after treatment compared with those before treatment,and the changes in observation group were more remarkable〔t-PA(mg/L):13.03±2.15 vs. 12.95±2.16,PAI(mg/L):23.64±9.07 vs. 26.81±10.00, Hcy(μmol/L):9.13±1.15 vs. 11.52±3.17,phlegm syndrome:9.16±1.71 vs. 11.17±2.89,fire-heat syndrome:7.51±1.59 vs. 8.81±2.26, sthenic-fu syndrome:0.61±0.87 vs. 1.19±1.14, NIHSS score:5.70±3.16 vs. 5.90±2.97〕. Conclusion The mechanism of Huatan Tongfu decoction in treatment of patients with acute cerebral infarction accompanied by TCM syndromes of phlegm heat and sthenic-fu may be related to the reduction of plasma PAI and Hcy levels,protection of vascular endothelium and promotion of fibrinolysis,thereby the decoction can improve the clinical efficacy.