Intestinal infection is one of the most common infectious diseases in children in China, and the intestinal infection is often featured as the diarrhea the main clinical symptom. Systematically understanding the etiological characteristics of intestinal infection in children in China and efficiently detecting the pathogens are crucial on the early treatment and prevention of diseases. The detection technology of clinical pathogenic microorganisms in China is undergoing a revolutionary era from microscopic examination to metagenomic sequencing.

Objective The effect of leech injection on experimental therombus formation in rats was studied. Methods The method of common carotid artery-external jugular vein by accessory pathway in rats was used. leech injection to observe its antithrombotic effect. At the same, related indecex were assessed. Results The leech injection inhibited the formation of experimental thrombus obviously and reduced aggregation of plate]et and adhesiveness of platelet. Conclusion It is suggested that leech injection has the effect of antithrombosis formation.

Language ; Periodicals as Topic ; Publishing

OBJECTIVETo analyze the status and papers information of Chinese Journal of Applied Physiology, and provide a guide for authors, readers and editing staffs.

METHODSWe retrieved papers of Chinese Journal of Applied Physiology from 2009 to 2013 from CNKI, and performed a statistical analysis of papers information contained in the text using bibliometrics method. Papers information such as document type, found support, author affiliation type, and discipline classification, etc. was analyzed.

RESULTSSeven hundred and forty two papers including 27 full English articles in total were published in Chinese Journal of Applied Physiology from 2009 to 2013. More papers were supported by national, provincial, and the other foundation, it was about 82.6% of all papers.

CONCLUSIONChinese Journal of Applied Physiology has stable manuscript resources, various discipline papers, and play an important role for development of applied physiology in China.

Objective To explore the development requirements and tentative ideas on the standardized teaching wards in the internship course of undergraduate nursing students. Methods 134 nursing undergraduate students before the internship and 70 nursing teachers were recruited in this study. A self-made questionnaire was distributed to these students and teachers to explore their requirements and needs in the development of the standardized teaching wards. Results The majority of students and nursing teachers thought that it was necessary to design and develop the standardized teaching wards for the nursing students and the six items of this study were accepted by them. Conclusion The standardized teaching wards will be designed and developed according to the six items of this study.

Objective To investigate relationship between microRNA-200c and ZEB1 in cervical epithelial mesenchymal transition factor. Methods 50 cases diagnosed with cervical cancer whose tumor tissue were taken to be the experiment group.50 cases diagnosed with uterine myoma whose normal tissue were taken to be the control group.ZEB1 and microRNA-200c of uterine tissue were were detected.Results The average level of ZEB1 in the experimental group was significantly higher than that of the control group (P<0.05), and in cervical cancer, lymph node metastasis and the depth of myometrial invasion had statistically significant (P<0.05).In experimental group, microRNA-200c average was lower than control group (P<0.05), and in cervical cancer, different pathological grade group, lymph node metastasis and deep muscular layer infiltration had statistically significant(P<0.05).In experimental group, ZEB1 and microRNA-200c showed negative correlation(r =-0.270 P =0.001).Conclusion The expression level of ZEB1 in cervical cancer is higher, and the expression level of microRNA-200c in cervical cancer is lower, suggesting that the two factors may play an important role in the invasion and metastasis of cervical cancer.The correlation between the two factors suggests that microRNA-200c may be involved in the process of cervical cancer by ZEB1.

Objective To explore the protective effect of atorvastatin on irradiated endothelium and the thrombomodulin(TM)expression.Methods Cultured human coronary artery endothelial cells(HCAEC)and human umbilical vein endothelial cells(HUVEC)were treated by atorvastatin at the final concentration of 10 μmol/ml for 10 min,and then irradiated with 2 and 25 Gy.Cell cycles status and TM expression were quantitatively measured by flow cytometry 24 hours after irradiation.Protein C activation in endothelial cells was also assessod.Results After administration with atorvastatin for 24 h,the TM expression increased by 77%,59% and 61% in normal control group,2 Gy group and 25 Gy group,respectively(t=27.395,26.420,58.065;P=0.000).The protein C levels decreased by 23% and 34% compared with the normal group post-irradiation to 2 and 25 Gy,but increased by 79% and 76% compared with the irradiated control group after administration with atorvastatin.The rates of cell apoptosis decreased by 6% and 16% in 2 Gy and 25 Gy groups,respectively after administration with atorvastatin for 24 h(t=4.178,17.863;P=0.000).Conclusions Atorva statin can protect endothelia cell from irradiation-induced apeptosis by increasing TM expression and protein C activation.

In the progress of the new healthcare reform, China has carried out a series of reform exploration in the drug distribution system.But fluctuating markups of drugs are still the main cause of high drug price.In order to simplify the distribution procedure, the National Health and Family Planning Commission proposed to encourage the implementation of two-receipt system nationwide.As the important part of the price system of drug circulation, drug distribution system made a great impact on the implementation of two-receipt system policy.Focusing on two provinces namely Fujian and Shaanxi provinces, this paper will discuss the typical drug distribution system in china.This paper has evaluated the main body and operation pattern in the implementation of two-receipt system, and by analyzing the benefits and drawbacks, in the same provinces.It has put forward the optimized distribution mode and provided policy suggestions for strengthening the two-receipt distribution system.

Chronic infection of hepatitis B virus(HBV)presents one of the serious public health challenges worldwide.Current treatment of chronic hepatitis B(CHB)is limited,and is composed of interferon and nucleoside/nucleotide reverse transcriptase inhibitors(NRTI).Interferon is poorly tolerated and is only responsive in a small fraction of CHB patients and NRTIs often face the problem of emergence of drug resistance during long-term treatment.The current treatment of CHB earl be improved in several ways including genotyping mutations associated with drug resistance before treatment to guide the choice of NRTIs and suitable combinations among NRTIs and interferon.It is important to continue research in the identification of novel therapeutic targets in the life cycle of HBV or in the host immune system to stimulate the development of new antiviral agents and immunotherapies.Several antivirai agents targeting HBV entry,cecDNA,capsid formation,viral morphogenesis and virion secretion,as well as two therapeutic vaccines are currently being evaluated in preclinical studies or in clinical trials to assess their anti-HBV efficacy.

Objective To explore the integrity of white matter changes in patients with vascular mild cognitive impairment (VaMCI)based Leukoaraiosis.Methods The patients which appeared white matter hyperintensities in subcortical were performed psychological testing,then 48 VaMCI patients and 18 without cognitive impairment volunteers(NC)were choosed and performed DTI scanning,to determine the white matter’s values of FA and ADC in the following bilaterally symmetrical regions of interest(ROI):entorhinal cortex lobe,hippocampal vault,anterior of corpus callosum,posterior of corpus callosum,inferior frontal lobe,superior temporal lobe and angular gyrus of occipital lobe. Then the differences of the data of DTI between two groups and the relevance between neuropsychological score and DTI’s data were analysed.Results ADC values were significantly higher of VaMCI group in the left inferior frontal lobe,left anterior of corpus callo-sum,left hippocampus vault and right angular gyrus compared with NC group(P <0.05),and in the left hippocampus ADC values and MoCA scores was negatively correlated(r=-0.34,P =0.02).There was no significant difference of FA values between groups. Conclusion The ADC values in many cognitive related areas was reduced in VaMCI patients.