Objective: To investigate the association between environmental exposure (living environment and production environment) to crocidolite and the cancer mortality risk. Methods: A cohort including 1 249 healthy residents recruited from crocidolite-contaminated area in Dayao County, Yunnan Province in 1984 was retrospectively analyzed. The COX regression model was used to estimate hazard ratio (HR ) and 95% confidence interval (CI ) for cancer mortality according to time of exposure to crocidolite and the crocidolite exposure in the production and living environments of blast-furnace manufacturing, material processing, whitewashing the wall, masonry stove building, and starching clothing with slurry. Results: A total of one hundred and twenty-one deaths were identified by April 30, 2011. After adjusting for the confounding factors including age, gender, marriage, education, smoking and alcohol consumption, it was found that as compared with population exposed to crocidolite in five years or less, HRs (95% CIs ) for cancer mortality in populations exposed to crocidolite in 6-10, 11-15 and more than 15 years were 2.898 (1.496-5.616), 4.159 (2.169-7.975), and 4.233 (1.182-9.886), respectively, and a dose-response relationship (P trend<0.05) was observed; compared with population not engaged in production of blast-furnace, the HRs (95% CIs ) for cancer mortality in populations engaged in sporadic production and mass production of blast-furnace were 2.533 (1.549-4.142) and 2.541 (1.642-3.935), respectively; compared with population not engaged in material processing, the HRs (95% CIs ) for cancer mortality in populations using crushing machine in material processing and handcrushing in material processing were 1.811 (1.211-2.709) and 1.785 (1.072-2.793), respectively; the HRs (95% CIs ) for cancer mortality in populations whitewashing the wall for 4 times or more, building the masonry stove and starching clothing with slurry were 1.736 (1.000-3.012), 2.229 (0.97-5.122) and 3.307 (1.522-7.184), respectively. Conclusion: Exposure to crocidolite in living environment and production environment may increase the risk for cancer mortality. Copyright © 2012 by TUMOR.

Objective To compare the analgesic and side effects of tramadol, buprenorphine and morphine in equipotent dosage in patient-controlled intravenous analgesia (PCIA). Methods 53 patients (ASA I-II) scheduled for upper abdominal surgery were randomly allocated to receive 10mg/ml tramadol (group T) or 0.03mg/ml buprenorphine (group B) or 1mg/ml morphine (group M) plus 0.1mg/ml droperidol. Baxter APII PCA pump was used to administer the different analgesic according to the model of LCP, with the amount of loading dose, continuous infusion and bolus of 2.5ml, 0.5ml/h, 1ml respectively. At 4, 8, 16 and 24h postoperatively, pain scores in VAS, comfort scale in BCS, sedation scores with Ramsay score, and the incidences of nausea, vomiting, pruritus, respiratory depression were assessed. Drug consumption in the first 24h and the D/D ratio (demand/deliver) were also recorded. Results VAS and BCS scores showed no significant difference among the 3 groups, but drug consumption within the first 24h and the D/D ratio in group T were higher than those in other 2 groups (P0.05). 8 patients in group T, 5 in group B and 4 in group M suffered from nausea; 1 patient in group T, 2 in group B and 3 in group M complained of pruritus. No respiratory depression was observed in all cases. Conclusion Tramadol and buprenorphine can be safely used as PCIA in patients undergoing upper abdominal surgery, but they are not superior to morphine.

40), the values of BIS, electromyogram (EMG), signal quality index (SQI), mean arterial pressure(MAP), and heart rate(HR) were recorded every minute for 5min, then additional vecuronium (0.05mg/kg) was administered, and the values of the parameters above mentioned were registered for the next 10min. Results BIS value decreased significantly 3 min after the administration of the additional vecuronium (P0.05). Conclusion A misleading BIS value may be caused by the decline of muscle relaxant during the maintenance stage of propofol/fentanyl anesthesia, which greatly influence the accuracy of BIS monitoring.

0.7?g/L (n=18) and group 2 ≤0.7?g/L (n=18). Propofol concentration, BIS, and AAI were compared between the two groups. Results The patients in group 2 had a significantly higher propofol concentration in plasma (2.70 mg/L) on awakening compared with that of the group 1 (2.08 mg/L) (P

Objective To compare the analgesic effect, adverse effects of patient-controlled epidural analgesia (PCEA) vs patient-controlled intravenous analgesia (PCIA) and their influences on stress response in thoracotomy patients. Methods 33 ASA I-II patients undergoing elective esophagectomy were randomly assigned to receive either morphine plus droperidol (Group PCIA, n=17) or morphine plus bupivacaine (Group PCEA, n=16) for 3 days postoperatively. Serum concentrations of cortisol, angiotensin Ⅱ (Ang Ⅱ), endothelin (ET) and blood glucose were measured before operation, at the end of the operation and on the next morning after operation. Pain scores with visual analogue scale (VAS) at rest, deep breathing and with cough, and adverse effects were also recorded. Results Blood glucose and cortisol were increased to similar levels in both groups at the end of the operation, but were much lower in Group PCEA postoperatively (P0.05). Pain scores with VAS were much lower in Group PCEA, especially when the patient was at deep breathing or during coughing (P

After murine fetal liver cells (FLC) were transfected with granulocyte-macrophage colony-stimulating factor (GM-CSF) gene by recombinant adenovirus and intrasplenically transplanted into allogeneic mice, the effects of GM-CSF gene-transfected FLC on the recovery of immune response inhibited by chemotherapy were observed. The number of CD4 + cells and the ratio of CD4 + /CDS + cells from peripheral blood lymphocytes increased significantly. The cytotoxicity of the NK cells and the proliferation response of splenocytes to ConA, LPS elevated markedly, but the same results were not from bone marrow. These data demonstrated that intrasplenic transplantation of GM-CSF gene-transfected FLC could effectively accelerate the recovery of immune response after high-dose chemotherapy.

40, of which 12 episodes were accompanied with body movement (42.8%) . BIS response was (84?19) seconds later while AAI response was (13?3) seconds later than body movement. Conclusion The relationship between BIS/ AAI and body movement is poor and both AAI and BIS respond later than body movement, so can not be used to predict body movement during propofol anesthesia.

Objective: The objective of this study is to understand the construction of laboratory testing capacity of the Chinese Disease Control and Prevention institutions at all levels and to expound the problems and puts forward the related suggestions and countermeasures which can provide the basis for scientific and corresponding improvement of laboratory construction.Methods: The whole procedures were completed through descriptive epidemiological studies using SAS 9.2 Software and Excel 2010 for conducting statistical and trend analyses.Results: There is an increasing trend in the number and proportion of inspections in the disease control institutions at all levels in China.The number of disease control and prevention institutions` special testing laboratories increased significantly in 2012 and then the growth rate undergone a slow-down.The owning rate of equipped laboratory appliance rapidly rose in 2011 and started to level off after that.The average number of equipment in eastern region is greater than that drawn in the central and western regions.According to rate of compliance with the standard of basic laboratory projects, there is a trend in the provincial, district and county level disease control and prevention institutions where the eastern region comes first, followed by the central and then the western region closes the line.However, in general, this rate of those provincial level institutions show a state that the central region holds the highest rate, the eastern region comes second, and the western last.Conclusions: The qualities of health inspection personnel still need to be further improved in disease control and prevention institutions at all levels in the country.In the aspect of laboratory equipment possession rate, the provincial-level CDCs have greater and absolute advantage than the county-level and district level institutions.The testing projects of CDCs in the central and eastern regions of China have a relatively good level, while those tracked in the western region are relatively weak.In view of this, China should strengthen the support to western region in terms of human resource, equipment and funding.The provincial-level disease control and prevention institutions` laboratory testing and diagnosis projects are the best in balance and fairness.Improving the testing capacity is an effective approach to promote the development of disease control and prevention.Hence, the state should pay much attention to the laboratory construction works and management strategies and flows.

Objective To develop a genetically modified fetal liver cells (FLC) based transplantation system that can release therapeutic levels of hematopoietic growth factors into the system circulation which can facilitate treatment of patient receiving cytokine therapy following chemotherapy. Method Examine adeno virus mediated gene transfer to isolated murine FLC and evaluate the biocharacterization of intrasplenic transplantation of gene modified murine FLC. Results Substantial transfection rate of 80%～85% were achieved at a ratio of 50 for 2 hr of exposure. Gene modified FLC (FLC GM) labeled with 111 In were injected into the allogenic mice, spleen, the %ID/g of liver was 20%～25% at 24 hr and 50%～55% at 48 hr after transplantation. In addition, serum concentration of GM CSF in mice with intrasplenic transplantation reached its maximum at 48 hr [(356 ?58 ) pg/ml]. Conclusion Intrasplenic transplantation of FLC GM can be predominantly localized in liver and spleen, and engraft rapidly and maintain normal function, which represent a critical step toward successfully accomplishing liver directed gene therapy.

[Objective]To explore the clinical effects of selecting differents type of tendon transplants to reconstruct anterior cruciate ligament(ACL) using press fit technique.[Method]A total of 101 cases of ACL injury were included in this study,62 cases were athletic injuries,22 cases were traffic injuries,and 17 cases were daily-life injuries.According to patient's condition using different types of tendon transplant in which hamstring-tendon-knot 42 cases,bone-bolt-hamstring tendon knot 32 cases,patella bone-quadriceps tendon 27 cases,the tendon-knot was completely fit-into the bottle-necked femoral tunnel,and the tibial tunnel were pulled ont and seperated into two bundles, then tie a knot to fix the tendons tightly onto the bone bridge.[Result]In this group 98 patients were followed-up,According to the knee joint effective evaluate standard,88 were excellent,6 good and 4 fine.Excellent and good rate was 85.7%.[Conclusion]According to patient's condition,different type of tendon transplant to reconstruct ACL by press fit technique should be selected carefully.It is an method of easy to manipulate,reliable fixation,minimized trauma,biological fixation,economical and free expensive implant technique.