1.Extracting Technology of Kanggan Qudu Ling Granule
Traditional Chinese Drug Research & Clinical Pharmacology 2001;12(2):118-119
Objective:To investigate the effects of various deco cting times,and frequencies on the main active components of Radix et Rhizoma Rh ei and Rhizoma Coptidis decocting together so as to determine the best extractin g technology.Methods:Orthogonal method was used to screen the extracting techno logy and TLC scanning to determine the content of main active components. Conclu sion: Proper decocting frequency and adding water volume will increase the yield of the main active components.
2.Effects of antioxidant on the gene expression of ?_1-adrenergic receptors during endotoxic shock in rats
Chinese Journal of Anesthesiology 1995;0(02):-
Objective To investigate the effects of antioxidant on the gene expression of ?1-adrenergic receptors (AR) during endotoxic shock. Methods Forty male SD rats weighing 200-250 g were anesthetized with intraperitoneal (i.p.) pentobarbital. The animals kept spontaneous breathing. Femoral artery and vein were cannulated for BP monitoring and drug administration. The animals were randomly divided into 5 groups ( n = 8 each) : (1) control group; (2) LPS group received IPS 15 mg?kg-1 i.v. ; (3) LPS + propofol group received at 1h after LPS a bolus of propofol 10mg?kg-1 i.v. followed by continuous infusion of propofol at 10 mg?kg-1?h-1; (4) LPS + uric acid (UA) group received uric acid 200 mg i.p. 1 h after LPS and (5) LPS + melatonin (MLT) group received MLT 10 mg?kg-1 i.p. 1h after LPS. The animals were killed at 6 h after LPS. Total RNA was extracted from the heart, aorta, vein, lung, liver and kidney. ?1A-,?1B-,?1D- AR mRNA and ?-actin mRNA were measured using reverse transcription polymerase chain reaction (RT-PCR) .Results The expression of ?1A-AR and ?1B-AR mRNA in all the organs and the ?1D-AR mRNA expression in aorta, liver, lung and kidney were strongly down-regulated in LPS group. In group 3 (LPS + propofol) the expression of ?1A-AR mRNA in the lung and kidney, the ?1B-AR mRNA expression in all of the organs and ?1D-AR mRNA expression in aorta, liver, lung and kidney were significantly increased as compared with LPS group. There was no significant difference in ?1-AR mRNA expression between LPS group and LPS + MLT group. The expression of ?1A-AR mRNA in kidney, the ?1B-AR mRNA expression in all of the organs except the lung and the ?1D-AR mRNA expression in aorta, liver, lung and kidney were significantly higher in LPS + UA group than in LPS group. Conclusion Circulatory failure induced by endotoxic shock is related to the down-regulation of ?1-AR gene expression. The therapeutic effects of antioxidant on the endotoxic shock is partly due to up-regulation of ?1-AR gene expression.
3.Discussion about Current Pathological Education as Abnormal Morphology
Chinese Journal of Medical Education Research 2006;0(08):-
With the rapid development of high science and technology,new teaching methods related to computer and electronic message techniques,have been applied to the recent medical education.Classical pathological teaching model has been gradually replaced by the modern way.This paper reports our teaching reformed practice by using the new technology and form the current educational theory.
4.Correlation between microalbuminuria and cardiovascular events in patients with coronary heart disease
Chongqing Medicine 2015;(24):3374-3375,3380
Objective To investigate the correlation between microalbuminuria and cardiovascular events in patients with coronary heart disease.Methods A total of 120 CHD patients were divided into experiment group (30 mg/g< UACR<300 mg/g, n=80)and control group(UACR≤30 mg/g,n=40)The relationship between MAU and cardiovascular events rate was compared after 1 5 months.Results Microalbuminuria level in experiment group was significantly higher than control group(P <0.05).The incidence of cardiovascular event in experiment group was significantly higher(t = 3.71 7,P <0.05 ).MAU and cardiovascular e-vents were positively related by Spearman′s analysis(r=0.721,P <0.01).The effective factors were MAU and CRP(MAU:P =0.001;CRP:P =0.037).Conclusion MAU concentration had a great impact on the incidence of cardiovascular event.The incidence of cardiovascular might be predicted by the MAU.
6.Effect of nitrotyrosine on the expression of ?1D-adrenoceptor mRNA in vascular smooth muscle of rat
Jing LI ; Liang JING ; Li-Jun LIAO ;
Chinese Journal of Anesthesiology 1994;0(01):-
Objective To investigate the effect of nitrotyrosine (3-NT) on the expression of ?1D adrenoceptor (?1D-AR) mRNA in cultured vascular smooth muscle cells (SMCS) .Methods SMCS were obtained from the tunica media of thoracic aorta of 1 month old SD rats and cultured in DMEM medium. The experiment consisted of two parts. In part Ⅰ SMCS were incubated with 0,1, 10, 100 or 200 ?mol?L-1 3-NT for 24 h and in part Ⅱ SMCS were incubated with 100 ?mol?L-1 3-NT forO,12, 24, 48 or 72 h. The total RNA was isolated by using Trizol reagent. The expression of ?1D-AR mRNA was determined by RT-PCR and agarose gel electrophoresis. Results In part I incubation with 1 and 10 ?mol?L- 3-NT for 24 h had no significant effect on the expression of ?1D-AR mRNA while incubation with 100 or 200 ?mol?L-1 3-NT for 24 h decreased the expression of ?1D-AR mRNA compared with 0?mol?L-1 3-NT (P
7.Current situation and influencing factors of patient safety culture among medical staffs in 4 hospitals of Shenzhen City
Jingyan LIAO ; Changpeng SUN ; Jing WANG ; Wangzhong CHEN ; Sizhao LIAO
Chongqing Medicine 2017;46(13):1801-1803,1806
Objective To analyze the status quo and influencing factors of patient safety culture among medical staffs in the hospitals of Shenzhen City to provided reference for its improvement and conduct the patient safety culture research by other medical institutions.Methods The Patient Safety Culture Questionnaire of Medical Institutions(PSCHO) was adopted to investigate on 1000 medical persons from February to May 2016.Results The total score of patient safety culture in medical staffs was (3.53±0.40) and,the difference among different categories of medical persons had no statistical significance (P> 0.05).The differences among the medical staffs with different working ages,communication frequency with patients,training times,concern frequency had statistical difference(P<0.05).Conclusion The patient safety culture level in Shenzhen City is higher.However,the sense of blame and shame is one of the important factors impeding the promotion of the patient safety culture in hospitnl.Hospital should conduct the targeted improvement to the related factors affecting the patient safety culture from the aspects of organism,department and individual level.
8.Role of vascular remolding induced by nitric oxide and matrix metalloproteinase in aneurysm
Mingfang LIAO ; Zaiping JING ; Jun ZHAO
Chinese Journal of Pathophysiology 2000;0(07):-
Nitric oxide (NO) and matrix metalloproteinases (MMPs) are important in vascular remolding, especially in abdominal aortic aneurysm. NO may be associated with aneurysms by modulating MMPs expression and activity. [
9.Clinical Study of Adjunctive Therapy with Mucosolvan Oxygen Atomization on Bronchitis
Jing LIAO ; Weiping WU ; Huifang PAN
Journal of Chinese Physician 2002;0(S1):-
Objective To study the adjunctive therapy with mucosolvan oxygen atomization on bronchitis. Methods All of the 83 cases were bronchitis patients in the hospital, and divided into 2 groups, we observed it`s curative effect compared with normal therapy. HZ Results Therapy in hospital mucosolvan group was 8.4 and thutinegroup was 11.2. Time of chirping and disappearing of lung phlegm.It is 5.1 to bathe Mucosolvan group. But routine group was 8.4.The two groups had remarkable differences and have statistics meanings. Conclusion Mucosolvan Oxgyen urge atomization is a kind of safe and high-efficcient way to heal the bronchitis.
10.An Investigation on Drinking Water Pollution by Vanadium and Titanium in Panzhihua
Hu YANG ; Qingping LIAO ; Jing WANG
Journal of Environment and Health 1992;0(02):-
0.05). The vanadium and titanium contents in surface water were higher than those in ground water (P