Objective To explore the diagnosis and treatment of Mycobacterium tuberculosis infection in a child with severe β Mediterranean anemia after allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods The clinical data of a child with severe β mediterranean anemia who had Mycobacterium tuberculosis after allo-HSCT were retrospectively analyzed.The pertinent literatures were reviewed.Results Six-year-old girl with Mediterranean anemia was infected by Mycobacterium tuberculosis after allo-HSCT.After anti tuberculosis treatment by HRZE (isoniazid,rifampicin,pyrazinamide and ethambutol),the condition was improved.Conclusion It is rare of Mycobacterium tuberculosis infection after allo-HSCT,which needs timely diagnose and treatment.

【Objective】To observe the effect of electroacupuncture(EA) on sperm count and quality in oligoasthenozoospermic rats.【Methods】Twenty-four male SD rats were given adenine by intragastral administration to establish oligoasthenozoospermic models.After the modeling,the survival rats were randomized into EA group(N=8) and model group(N=8).EA group was treated with EA of Guanyuan(CV4),Shenshu(BL23) and Sanyinjiao(SP6) acupoints.Other 10 rats served as the normal controls.Sperm samples were analyzed by computer-aided semen analysis system(CASA) and the observed sperm parameters included sperm concentration,semen viability and percentage of forward progressive sperm.【Results】Compared with the normal group,sperm concentration,semen viability and percentage of forward progressive sperm were much decreased in the model group(P

0.05). The absorbance value of Trichinella-infected muscle conserved at -20 ℃ for 10 wk decreased to 0.43, with significant difference from that conserved at -20 ℃ for 1 wk, but the positive rate was also 100%, and antibodies were detected in all muscle samples conserved at -20 ℃ for 20 weeks when the experiment was ended. Conclusion When animals died or were slaughtered and serum samples could not be collected, muscle juice can be collected from fresh, cool and frozen meat and used as a substitute sample for detecting anti-Trichinella antibodies.

0.05).The scales of subtests including arithmetic,digit span,picture completion,block design and coding of children in CAE group were significantly lower than those of control group(Pa

Aged ; Antibodies, Monoclonal ; metabolism ; Carcinoma, Transitional Cell ; metabolism ; pathology ; surgery ; Cystectomy ; Follow-Up Studies ; Humans ; Keratin-7 ; metabolism ; Keratins ; immunology ; Lung Neoplasms ; secondary ; Male ; Urinary Bladder Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective:To investigate the role of clinical pharmacists in the anti-infective treatment of one patient with complexity urinary tract infection with septic shock induced by cellulitis. Methods:Clinical pharmacists participated in one case of complexity uri-nary tract infection with septic shock induced by cellulitis, and according to the clinical curative effect and the patient' s condition change, clinical pharmacists adjusted the medication nine times and provided individualized pharmaceutical care and service in the whole process. Results:Physician accepted the suggestions of clinical pharmacists, the infection was controlled after the 38-day treat-ment, and then the patient was discharged from the hospital. Conclusion:Clinical pharmacists should participate in the clinical treat-ment, provide whole process of pharmaceutical care for severe patients and assist physician in drug treatment decisions to promote safe, effective and economical drug use.

Graduation project is key link in the cultivation of innovative talents for college teaching work,which is the need of the era.Thus,this paper introduced the current development of biomedical engineering at home and abroad,and the achievement of the graduation project of our university at present.Then,the necessity of the teaching reform and innovation of graduation project are analyzed.The methods for the teaching reform and innovation of graduation project are introduced including the choice of projects,the adjustment of the project schedule,reinforcing the practice of projects and improving the ability to apply knowledge,the assessment criteria of grades for the project,etc.

With Minglou Community Children's Rehabilitation Base as a case,which was jointly founded by tertiary specialized hospital and community health service center,the paper introduced how to use SWOT model to systematically analyze the strengths and weaknesses of the internal environment,opportunities and threats of external condition.Based on the analysis,the solutions,suggestions and policy of children's rehabilitation were proposed,providing reference for decision making to promote the community children's rehabilitation.

Objective To establish a method of sperm DNA extraction in mixed stain by using DNase-Ⅰ purificationcombined with alkaline lysis method in forensic science.Methods 79 mixed stain samples of criminal cases were collected.Sperm DNA was extracted using the purification of DNase-Ⅰ binding alkaline lysis method.16 STR loci were genotyped with fluorescent multiplex amplification system.The typing results were compared with that of extracted using two-step differential extraction procedure.Results Of all 79 mixed stain samples,64 samples were genotyped successfully by using DNase-Ⅰ purification combined with alkaline lysis method while 57 samples were genotyped successfully with two-step differential extraction procedure.There was significant difference between two methods(P=0.039).The purification of DNase-Ⅰ binding alkaline lysis method had a higher success rate and lower cost than that of two-step differential extraction procedure.Conclusion Purification of DNase-Ⅰ binding alkaline lysis method can increase the typing success rate of the mixed stain samples.The method is simple,rapid and easy to be automated,and suitable for forensic identification test.

Objective To select an optimal non-specific antigen blocking method by using immuno-infiltration assay so as to suit protein chip preparation. Methods Human papillomavirus type 16 L1 protein expressed by insect-baculovirus espressin system was incubated with skimmed milk powder, calf serum, bovine serum albumin (BSA) combinations of five kinds of methods to block the non-specific antigen. PBS was used as control. The effect of eliminating non-specific stain was detected by immuno-infiltration assay. Results After repeated tests, the results showed that the stability and repeatability of blocking effects were poor for the fixing up antigen first and then blocking method, and the blank control was prone to false positive. The infiltration rate of NC membrane would be affected by using skimmed milk powder as a blocking agent because the pore of NC membrane was easily plugged by milk powder particles. The use of calf serum as a blocking agent made it very difficult to determine the result because the calf serum absorbed by NC membrane produced the background; however, when 20g/L BSA was used to blocking before fixing up antibody, the results became satisfactory. Conclusion Fixing up antibody after blocking in immuno-infiltration assay showed that the blocking effect against non-specific antigen was satisfactory, stable and repeatable, indicating this method is a novel optimal blocking method compared with others.