1.Effect of high glucose on the expression of activating transcription factor 4 in cultured retinal Müller glia cells
Chinese Journal of Ocular Fundus Diseases 2012;28(5):498-500
Objective To observe the effect of high glucose on the expression of activating transcription factor 4 (ATF4) in cultured retinal Müller glia cells.Methods The retinal tissue of Sprague Dawley (SD) rats was collected,and Müller cells were isolated and cultured.The glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS) of Müller cells were identified by streptavidin-biotinperoxidase complex.Cultured rat Müller cells were divided into control group (5.5 mmol/L glucose),group A (20 mmol/L glucose),group B (30 mmol/L glucose) and group C (40 mmol/L glucose).ATF4 protein expressions in Müller cells of four groups were measured by Western blot four days after cultured.Results GFAP and GS expressed in more than 95% of Müller cells.Over 95% of Müller cells of group A,B and C were positive for GFAP and GS.Western blots indicated that ATF4 protein in group A,B and C increased obviously compared with the control group (q=0.293,0.754,0.484; P<0.05).Conclusion High glucose can increase the expression of ATF4 protein and cause endoplasmic reticulum stress in retinal Müller glia cells in vitro.
2.Risk factors analysis of postoperative intracranial infection in patients with recurrent glioma.
Chinese Medical Journal 2013;126(9):1796-1796
Brain Diseases
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etiology
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Brain Neoplasms
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surgery
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Glioma
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surgery
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Humans
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Infection
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etiology
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Postoperative Complications
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etiology
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Recurrence
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Risk Factors
3.Livedoid vasculitis: an update
Chinese Journal of Dermatology 2016;49(5):371-374
Livedoid vasculitis is a kind of skin disease caused by local vascular occlusion.It mainly manifests as erythematous and purpuric maculas or papules on the legs and ankles,which may progress into intensely painful ulcers and heal leaving porcelain-white atrophic scars called atrophie blanche.Histopathologically,inflammatory response is weak or absent in local lesions of livedoid vasculitis,hence,it is commonly considered to be related to local thrombosis and other autoimmune diseases.Now,it is mainly managed with anticoagulation-based comprehensive treatment in clinic.
5.Analyses of essential oil from Ferula sinkiangensis K.M.Shen
Shan WANG ; Jing KE ; Xufeng PU
Chinese Traditional Patent Medicine 1992;0(04):-
AIM:To establish a qualitative fingerprint analysis with capillary GC. METHODS:HP-INNOWAX column(30 m?0.53 mm,1.0 ?m),column temperature:90 ℃(15 min)4 ℃/min180 ℃(5 min)2 ℃/min200 ℃(3 min)10 ℃/min220 ℃(5 min),FID detector temperature:230 ℃. RESULTS:Compared with reference peak of Sec-butyl-cis/trans-1-propentyl disulfide,17 common peaks on fingerprints of the oil from ferula sinkiangensis K.M.Shen were indicated by the method with satisfied stability,precision and repeatability. CONCLUSION:There are good similarities among 10 batches of essential oil from Ferula sinkiangensis K.M.Shen,and this method is reliable,simple and provides a reference standard for the quality control of Ferula sinkiangensis K.M.Shen.
6.Gladness and troubles for using garlic as an anti-oxidant.
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(5):462-463
Allyl Compounds
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chemistry
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pharmacology
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toxicity
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Animals
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Antioxidants
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chemistry
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pharmacology
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toxicity
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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toxicity
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Garlic
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chemistry
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toxicity
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Humans
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Phytotherapy
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adverse effects
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Sulfides
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chemistry
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pharmacology
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toxicity
7.Satisfactory investigation of bandage contact lens on patients after pterygium surgery
Chinese Journal of Practical Nursing 2016;32(3):196-199
Objective To evaluate the clinical effect of bandage contact lens on patients after the pterygium excision surgery.Methods 200 patients with pterygium excision were used retrospectively analysis and were divided into control group and observation group by random digital table method.Patients in observation group wore bandage contact lens while those in control group did not (traditional method).The degree of pain in both groups was evaluated at 8 hours,24 hours and 48 hours after surgery.In addition,patients were surveyed for the satisfaction of our treatment when discharge.Results The ocular foreign body sensation,eye burning,tears and eye open difficult were obviously decreased in observation group (t=5.643,10.315,P < 0.01).The overall comfort level of the patients in observation group was obviously improved.pain feeling of the eye after surgery in 8h,24h and 48h markedly decreased in observation group (t=6.196-12.495,P < 0.01).The degree of satisfaction scores of preoperative guidancein,postoperative pain control,emotion,mental nursing,self-care ability,comfort in observation group were (1.59-0.62) points,(1.83±0.70) points,(1.42±0.68) points,(1.42±0.68) points,(1.48±0.67) points,(1.29±0.77) points,(1.43-0.64)points which were obviously increased than (0.76±0.55) points,(0.83±0.64points,(0.82±0.56) points,(0.80±0.47) points,(0.79±0.61) points,(0.66±0.54) points (t=4.311-9.354,all P< 0.01).Conclusions Compared with traditional treatment,bandage contact lens can significantly reduce the discomforts after the pterygium excision surgery,and increase the postoperative comforts of patients.It proves that bandage contact lens have extensive clinical value.
8.Advances in biological prognostic indicators of chronic lymphocytic leukemia
Lan MA ; Jing WANG ; Xiaoyan KE
Journal of Leukemia & Lymphoma 2010;19(1):55-58
The patients of chronic lymphocytic leukemia(CLL)have great individual differences.It is important for clinicians to determine the prognosis at the beginning of diagnosis and choose proper treatment for the patients.The recent advances in biological prognostic indicators of CLL were reviewed,including IgVH gene mutation status,ZAP70,CD_(38),chromosome abnormality[t(11q;v),del(11q),del(17p),+12 and del(13q)].telomere and telomerase.
10.Research progress in the biology of Hodgkin's lymphoma in the 54th ASH annual meeting
Wei ZHANG ; Jing WANG ; Xiaoyan KE
Journal of Leukemia & Lymphoma 2013;22(7):385-386
The Hodgkin and Reed/Sternberg (HRS) tumor cells of classical Hodgkin's lymphoma (HL) and the lymphocyte-predominant (LP) tumor cells of nodular lymphocyte-predominant HL are both derived from germinal center B cells.Several studies show that HL pathogenesis includes the deregulated activity of numerous members and regulators of the NF-κB and JAK/STAT signaling pathways,genetic lesions of epigenetic regulators,and the interactions between tumor cells and other infiltrating cells in the microenvironment,et al.