Objective To study the immune state and correlated strategy of patients with hepatic cirrhosis and primary hepatic carcinoma.Methods 17 patients with hepatic cirrhosis and 8 patients with primary hepatic carcinoma(PHC) in our hospital were collected since 2002.The indexcs of immune function were detected in all patients and some were re-examined.Results It was found that although the immune index varied in patients suffering from severe liver diseases,some regularities were shown as follows:(1)CD4 and CD4/CD8 in T lymphocyte subset declined markedly(P

Though prokaryotic cells could hardly express recombinant human beta nerve growth factor (rhNGF-?) with a proper three-dimensional conformation, using of E. coli as a host for industrial production of rhNGF-? is controversial. Recombinant human beta NGF was expressed in E. coli and was refolded in vitro. The isolated products was shown to be consistent with those expressed and secreted by CHO cells in biochemical characters by SDS-PAGE, RP-HPLC, mass spectrometry, N terminal analysis and bioassay determined using DRG and PC12 cells. The products can be acquired with 95% purity, 1.8 ng/U biological activity from both expression system,which remain invariable biological activity when lyophilized in excipient and store at 37℃?2℃, RH 75%?5% for 3 months. Moreover, the product refolded from inclusion bodies of E. coli shows the predominance in homogeneity and the lower cost.

Objective: To summarize our experience on perioperative anesthesia management of patients with crush syndrome caused by earthquake under field condition. Methods: The clinical anesthesia data of 10 patients with crush syndrome caused by China Wenchuan earthquake, who were treated under field condition, were retrospectively analyzed, and our experience on perioperative anesthesia management was summarized. Results: After volume therapy, urine alkalization, and stabilization of internal environment, the 10 patients underwent debridement, decompression by fasciotomy or amputation under epidural anesthesia or brachial plexus block plus psychological therapy and moderate tranquillizer. During the operation, the patients showed smooth respiratory and cardiovascular characteristics without pain. Conclusion: Under field condition, regional anesthesia can provide adequate analgesic effect during and after the surgery. Early fluid resuscitation and life support are vital to the safety and life of patients.

Objective To discuss the application value of multi- position radiography in the diagnosis for fracture of rib.Methods 98 cases with integrated data of thoracic trauma undergone multi- position view X-ray from 2007 to 2009 were retrospectively reviewed.Results The percent of fractures with orthophoria view is 65.4%.The percents of fractures with oblique position,tangential projection and spot film under the X-ray fluoroscopy are 20.6%、5.1% 、8.8%,while doesn' t find the A-P position,The total percent is 34.6%.Conclusion Multi-position radiography can elevate the diagnosis for fracture of rib and has application value.

Objective:To explore the application value of continuous positive airway pressure ( CPAP) in the treatment of obstruc-tive sleep apnea hypopnea syndrome (OSAHS ) complicated with coronary heart disease (CHD).Methods:Totally 50 patients with OSAHS complicated with CHD from June 2012 to June 2014 in our hospital were randomly divided into the control group and CPAP group with 25 ones in each .The patients in the control group were treated with the conventional drug therapy for coronary heart disease , while those in CPAP group were treated with CPAP additionally .After 6-month treatment, PSG index, clinical symptoms, arrhythmia and ischemia electrocardiogram changes of the patients in the two groups were compared .Results:After the 6-month treatment , AHI and oxygen desaturation index in CPAP group were significantly decreased when compared with those before the treatment , and the min-imum value of SaO2 and the average value of SaO 2 were higher than those before the treatment (P<0.01), and the above indices were better than those in the control group (P<0.01).The incidence rate of chest pain and chest tightness , arrhythmia, times of myocardi-al ischemia in a day and time of myocardial ischemia in a day were decreased after the treatment (P<0.01), and the decrease in CPAP group was more notable than that in the control group (P<0.01).Conclusion: CPAP combined with the conventional drug therapy for coronary heart disease can improve the oxygen concentration and reduce the incidence of arrhythmia and myocardial ischemia in the patients with OSAHS with CHD , which can be widely used in clinics .

Firstly, this paper analyzed the significance of strengthening medical students’ ecological moral ed-ucation under the view of Beautiful China. Secondly, it discussed the present situation of ecological moral and the reasons of lacking of ecological moral in medical students. At last, the authors pointed out the countermeasures to strengthen the moral cultivation of medical students’ ecological countermeasures under the view of Beautiful China. Therefore, we must strengthen the top-level design to ensure the ecological moral education enter into the national education system, enhance to maintain power to build the full-area coverage ecological moral teaching system, and strengthen institutional construction to establish long-term mechanism of ecologic moral education.

Objective: To evaluate the clinical effect of individualized enteral nutritional (EN) support in severe stroke patients.Methods: Totally 120 severe stroke patients with nutritional risk were divided into two groups according to single or double admission number.The 60 patients in the intervention group received an individualized EN with different formula and concentrations according to the clinical status,and supplemented trace elements as occasion required.In the routine group, the patients received EN with standard formula.The energy supply was 83-125 kJ· kg-1·d-1 in both groups.Blood albumin(Alb), pre-albumin(PA), hemoglobin(Hb) and vitamin C(VC) were examined before and after the 21-day nutritional support.The lung infection and gastrointestinal (GI) complications were observed, and the hospital stay and cost were recorded as well.Results: After 21 day''s nutrition support, the serum levels of Alb, PA and VC in the intervention group were not statisticly significant than before (P＞0.05), and the serum levels of Alb, PA and VC were significantly higher than those in the routine group(P＜0.05).The incidence of complications in the in the intervention group was lower than that in the routine group (P＜0.05), and the length of hospital stay and hospitalization was shorter than that in the routine group (P＜0.05).Conclusion: Individualized enteral nutritional support can significantly improve nutritional status, reduce inflammation reactions and GI complications in severe stroke patients, which is worthy of clinical use.

Microcirculation is closely related to occurrence and development of disease, so it is of significance in noninvasive imaging of microcirculatory structure and function in vivo.Photoacoustic imaging is one of the main techniques in microcirculation imaging, which has the characteristics of high contrast, high detection depth and high sensitivity to tissue function.A review of the literature showed that photoacoustic technique can noninvasively image and quantitatively measure microcirculatory structure, vasoconstriction and vasodilation, concentration of hemoglobin, oxygen saturation, blood velocity and direction, oxygen metabolism level in a certain depth of living tissue in real time.Research progress in photoacoustic imaging of microcirculation in subcutis, cerebral cortex, tumor, eye and other tissues is reviewed in this paper.

Objective To explore the influence of breast cancer treated with chemo-therapy and Shengxue decoction.Methods 91 Cases of breast cancer were randomly recurited into two groups:The western medicine treatment group (40 patients) was given anastrozole tablets and chemo-therapy; The Chinese and western medicine treatment group (51 patients) was given chemo-therapy and Shengxue decoction.Results The total effective rate of was 82％ and 53％ in the Chinese and western medicine treatment group and western medicine treatment group respectively,the difference was statistically significant (x2=19.74,P＜0.05).The peripheral blood's CEA and MMP-2 mRNA expression of western medicine treatment were [ (7.50 ±4.32)ng/ml、(116.32±67.40)ng/ml respectively],higher than the Chinese and western medicine treatment group [ (7.20 ± 3.22) ng/ml、(105.08 ± 37.82) ng/ml,respectively,P＜ 0.05 ],but the TIMP-2/MMP-2 (26.65 ±19.75) was significantly lower compared with the Chinese and western medicine treatment group [ (39.48±21.21) (P＜0.05) ].Conclusion The combination of traditional Chinese and western medicine was effective in treatment of breast cancer and well worth for popularization.

Objective: To inquire into the discrepancy in the behavior and therapeutic ways for the children at different age with dental diseases.Methods: 994 children at the age of 1～12 with dental diseases were divided into 3 groups on the base of their ages. Relevant therapeutic ways were taken according to their behavior. The influencing factors that made dental treatment finished smoothly were reached through analyzing the relationship among the children, the parents and the medical personals. Results: The manifestations of children's behavior had significant age differences when they received dental treatment. Non cooperative children constituted the majority of the infantile children, while the school age children were mostly cooperative. The ways chosen to treat children's dental diseases also had significant age differences. Compulsory means was the main way to treat infantile children and routine means to the pre school age and school age children. Inductive and threatening ways were always used for the non cooperative pre school age children. The non cooperative school age children were always received inductive means and seldom threatening and compulsory ones. The parents often accompanied with the infantile children, but did not with the pre school age and school age children. Conclusion: The age is a key factor to determine the clinical manifestations and the methods used to treat children's dental diseases.