Objective: To study correlation among serum levels of lipoprotein a[Lp(a)], uric acid (UA) and cardiac function in patients with acute myocardial infarction (AMI) complicated diabetes mellitus (DM).Methods: A total of 70 AMI+DM patients were enrolled as AMI+DM group, 60 pure AMI patients were enrolled as pure AMI group, 60 pure DM patients were enrolled as pure DM group, and 60 healthy people were selected as healthy control group.According to NYHA cardiac function class, AMI+DM patients were divided into class I~II group (n=58) and class III~IV group (n=12);according to their prognosis, they were divided into no cardiovascular event group (n=54) and cardiovascular event group (n=16).Serum Lp(a) and UA levels were measured in all group, and their correlation were analyzed.Results: Serum Lp(a) and UA levels of AMI+DM group were significantly higher than those of healthy control group, pure AMI group and pure DM group, and those of pure AMI group and pure DM group were significantly higher than those of healthy control group, P<0.01 all;Pearson compared with class I~II group, there were significant rise in serum levels of Lp(a)[(183.28±19.96) mg/L vs.(335.65±22.71) mg/L]、UA[(287.45±21.82) μmol/L vs.(437.79±26.72) μmol/L]in class III~IV group (P<0.01 both);compared with no cardiovascular event group, there were significant rise in serum levels of Lp(a)[(220.75±25.72) mg/L vs.(423.97±30.81) mg/L]and UA[(308.49±21.82) μmol/L vs.(442.97±29.75)μmol/L]in cardiovascular event group, P<0.01 both;Pearson correlation analysis indicated that serum Lp(a), UA levels were significant positively correlated with cardiac function class (r=0.410, 0.412, P<0.05 both), and serum Lp(a) level was significant positively correlated with UA level (r=0.496, P=0.023) in AMI+DM patients.Conclusion: Serum Lp(a), UA levels are closely correlated with cardiac function in AMI+DM patients, which can be used as indexes diagnosing and monitoring disease progress.

Objective To investigate the relationship between gastroesophageal flap valve (GEFV) and reflux esophagitis(RE).Methods We analyzed 239 cases of RE diagnosed by endoscopy in our hospital from January 2007 to December 2009.RE was classified by Los Angeles classification system and GEFV by Hill system.The association of GEFV with RE was analyzed.Results The overall incidence of abnormal GEFV was32.9% (316/960) and that of RE was 24.9% (239/960).The incidence of severe RE ( grade C and D) in abnormal GEFV group was significantly higher than that in normal GEFV group (23.1% vs.2.4% , P = 0.000).RE grades were positively correlated with GEFV grades (r = 0.308, P = 0.000 ).The incidence of abnormal GEFV in RE patients was higher than that in non-RE (65.3% vs.22.2% ,P = 0.00 ).In terms of gender, there were more males than females in both RE group (63.6% vs.36.4% ) and abnormal GEFV group (60.1% vs.39.9% ).Additionally, incidence of RE increased with age, i.e.positively correlated with age (r=0.214,P =0.000).The incidence of abnormal GEFV also increased significantly in those over 30 years old, i.e.positively correlated ( r = 0.129, P = 0.000).Conclusion There is correlation between GEFV abnormality and RE.GEFV provides useful information for assessment and prediction of the reflux status of the patients.

Objective To diagnose limb-girdle muscular dystrophy type 2A(LGMD2A)using Western blot with micro-sample.nethods Skeletal muscle specimens of 73 cases of progressive muscular dystrophy with prominent proximal muscle involvement were obtained by open biopsy,and underwent histochemical,immunohistochemical stains with anti-dystrophin-N,C,R,α,β,γ,δ-sarcoglycan,dysferlin,caveolin-3 monoclonal antibodies.Twenty-nine cases were excluded with deficiency of dystrophin,sarcoglycans,dysferlin and caveolin-3,then Western blot and immunoreaction were performed with anti-calpain-3,caveolin-3 monoclonal antibodies.Results Ten cases with LGMD2A were diagnosed,all presenting muscle weakness of the proximal limb and elevated creatine kinase in vacant levels.and electromyography showed myogenic change.Histochemical stains showed variation in fiber size,different extent of degeneration,regeneration and necrosis,increased connective tissue elements,while lobulated fibers presented in 6 cases.Immunohistochemical stains identified dystrophin,sarcoglycans,dysferlin and caveolin-3 expressed normal in muscle fibers.Western blot,compared with the control,confirmed that the bands at 94 000 were absent(8 cases)or partly(2 cases)deficient:The additional bands at 30 000expressed weakly;The bands at 22 000 were normal.Conclusion Western blot analysis with microspecimen of skeletal muscle is an effective modality to diagnose LGMD2A,and is suitable to diagnose the sub-type of LGMD in clinic.

Objective: To study the effects of mechanical strain on the proliferation of human osteoblasts. Methods: Cloned MG 63 human osteoblasts were cultured and seeded at 10 5/well in 6 well Bioflex cell cuture plates. Subsequently, the cells were exposed to mechanical strain stimulation (at 6%, 12% and 24% elongation rate respectively) for 24 hours using Flexercell 3000 Cell Stretching Unit. MTT colorimetric method was used to assess the proliferation of the cells. Results: The proliferation of MG 63 cells was significantly increased 24 h after mechanical strain treatment( P

Platelet activating factor(PAF), a potent phospholipid mediator of inflammation, has diverse physiological actions and participates in many diseases in vivo . In recent years, it has been revealed that PAF system involve in the expression of keratinocytes function and reaction of skin inflammation. Therefore, PAF system was found closely associated with inflammatory skin disease. Keratinocytes could express functional PAF receptors (PAF R) and involve in the action of epidermis cytokine system. The interaction between PAF system and other inflammatory mediators can lead to skin inflammatory cascade.

Objective To explore the MRI features of brain and spinal cord in patients with multiple sclerosis(MS).Methods The data of MRI of 110 patients with clinic definite MS were analyzed retrospectively.Results The lesions on brain MRI were usually seen in the white matter surround the ventricle(55.8%),deep frontal lobe white matter(54.7%),deep parietal lobe white matter(44.2%)and brainstem(25.6%).Basal ganglia(23.3%)and thalamus opticus(11.6%)were also affected.The size of the brain lesions was varied,the appearances were punctiform,mottling,patching,ellipse and so on.The lesions of the spinal cord were usually on the cervical cord(75.0%)and thoracic cord(68.8%).There were string lesions which parallel spinal cord long axial and mottled lesions.The spinal cord lesions existed in both white matter and gray matter.10.0% of the patients had spinal cord atrophy or spinal cord swelling.The signal intensity of lesions was equal T1,long T2 or long T1,long T2,which was related with the clinical course.Conclusion Different size and appearance lesions in the white matter of brain and spinal cord are the main MRI features of MS.

Objective To study the regulating effect of complementing calcium and invigorating kidney method on the bone morphogenetic protein-4(BMP-4) and signal transduction mechanism.Methods Female Wistar rats were randomly divided into seven groups:normal group,model group,low-,middle-,high-dose yster calcium compound Chinese medicine for reinforcing kidney group,Gushukang granule group,oyster shell calcium tablets group.The model of postmenopausal osteoporosis was established through ovariectomy in rats.The osteoporosis rats were treated with nanometer calcium and invigorating kidney Chinese medicine Gushukang granule,and oyster shell carbonate chewable tablets were used as positive-control groups,the normal rats as standard control,and the model rats as model control.After 12 weeks of the treatment,the indexes were tested.The bone mineral density(BMD) of femoral bone in vitro was detected by dual-energy X-ray densitometer and the expression of BMP-4 and Smad 5,6 mRNA were detected by reverse transcription-polymerase chain reation(RT-PCR).Results Compared with the model rats,BMD of femoral bone in Chinese medicine groups(high dose,middle dose,low dose) increased obviously(P

Objective To analyze the center effect in correlation between the scores of Qi-Yin deficiency syndrome(SQYD)and blood sugar level in diabetes. Methods Correlation, consistency reliability and distinguish ability analysis were used to evaluate the correlation of the scores of Qi-Yin deficiency syndrome and blood sugar level according to the data from a clinical trial in phase Ⅲon the diabetes. Results There was a significant correlation between fasting blood sugar (FPG)level and 2-hour blood sugar (2 hPG)level at baseline, treatment end point and difference before and after treatment, and the variation of this correlation was slight (CV=0.1～0.258).But there was a insignificant correlation among SQYD and FPG or 2hPG, and the variation of this correlation was obvious (CV=0.560～2.559).The correlation coefficient among SQYD and FPG fluctuated in the range of -0.096～0.611,and that among SQYD and 2hPG in the range of -0.150～0.537. Roughly the variation of correlation among SQYD and FPG or 2hPG was 5～10 times greater than that of between FPG and 2hPG. Conclusion There exists an obvious center effect in correlation between the scores of Qi-Yin deficiency syndrome and blood sugar level in diabetes.

Objective To study angelica polysaccharides (APS) for mobilizing peripheral blood hematopoietic stem cell in BALB/c mice. Methods Techniques of peripheral white blood cell (WBC) and lymphocyte (LC) count, immunocytochemistry, flow cytometry, and hematopoietic cell culture in vitro were used to observe the APS mobilization. Results The numbers of WBC and LC in APS group were markedly higher than that in NS group (P

About half of the world population and more than 90% of rural households in the developing countries still use unprocessed biomass fuels (wood, dung and crop residues) as the fuels. These substances, burning in the open fire or in the simple stoves, can cause indoor air pollution and the pollutants exposure in women and children is more serious. Biomass smoke contains lots of toxic and harmful substances which may impact the health of human. This article presented a recent research progress in the indoor air pollution and health effects caused by combustion of biomass fuels in China and in the world.