Objective To explore the clinical curative effect of traditional Chinese medicine enema,amino salicylic acid preparation and probiotics in the treatment of ulcerative colitis.Methods 168 patients were selected as the research objects which were confirmed by endoscopic examination and the diagnosis of ulcerative colitis patients,randomly divided into the control group of 84 cases and observation group of 84 cases.The control group was given with sulfasalazine(SASP) treatment,a dose of 1g,4 times/d,period of treatment was 28d.In the observation group,oral SASP treatment combined with microecological preparation often meian and traditional Chinese medicine enema,Chang An capsule,2 capsules (500mg)/time,3 times/d,postprandial oral; Herbal Enema with angelica,white lentils,flavescent sophora root,cicada,windproof suffering after filtering,every night before bed retention enema.The clinical efficacy was compared between the two groups.Results After the treatment,the Sutherland index of the observation group was (1.82 ± 1.65),which was significantly lower than (5.51 ± 1.59) of the control group (t =10.436,P ＜ 0.05).In observation group,the clinical total effective rate,the total efficiency of endoscopic and histological total effective rate was 88.1％,81％,76.2％,the control group were 66.7％,57.1％,50％,the two groups had significant difference(x2 =4.353,4.512,5.113,P＜0.05).Conclusion The traditional Chinese medicine enema,amino salicylic acid preparation and probiotics in the treatment of ulcerative colitis has good curative effect,can relieve the clinical symptoms of patients,improve the degree of intestinal diseases.

The catabolic response to severe injury , burn, inflammatory ,acute pancreatitis and operation is characterized by whole-body protein loss, mainly reflecting increased breakdown of muscle proteins. Glucocorticoids and various proinflammatory cytokines are important regulators of muscle proteolysis in stressed patients. In recent years, three types of treatments have been used to reduce or prevent the catabolic response to injury and sepsis: ①nutritional, ②hormonal, and ③pharmacologic. This article review the catabolic response and the corresponding interruption under the stress conditions.

Objective To investigate the Diane -35, femonston of missed abortion endometrial repair. Methods According to the different treatment methods will be January 2015 to March 2017 in the first affiliated hospital of Tianjin university of traditional Chinese medicine 60 cases of patients with missed abortion groups: control group with -35 treatment, the observation group with femonston treatment; detailed observation of the two groups patients with various treatment index (bleeding time, bleeding volume, postoperative day 21 endometrial thickness, the tide time, the tide period), the occurrence of adverse reactions, and the related data were analyzed. Results Femonston (observation group) in treatment of patients with missed abortion is better than - 35 (control group) treatment, patients with various treatment index than the control group, the incidence rate of adverse reaction was lower than the control group, the difference was statistically significant (P<0.05). Conclusion Femonston can effectively repair the endometrium of patients with missed abortion, and shorten the time with the tide, the tide of stable period it is worthy of widely used intervention in patients with missed abortion.

Objective:To summarize the drug repercussion data in outpatient pharmacy and analyze the reasons to provide sugges-tions for the improvement. Methods: The information was collected from the hospital information system ( HIS) during January 1, 2013 and December 31, 2015, including drug name, category, quantity, amount, reasons for drug repercussion and the other statisti-cal data. Results:During the three years, the number of prescription drugs, amount of money and varieties of drug repercussion were increased. The most common reasons for drug repercussion were the other reasons, withdrawn by doctors and the request of patients. The sum of drug repercussion was positively correlated with the drug price and the amount of drug repercussion. Conclusion:The op-tion simplicity for drug repercussion in HIS is the main reason for drug repercussion bythe other reasons. The occurrence of drug re-percussion should be intervened by such methods as gradually improving the HIS, establishing intervention measures, enhancing the professional quality of pharmacists and so on.

Root stem of Anemarrhena asPhodeloides was extracted with hot water,treated with ethanol and dilutealkali to temove protein. The polysaccharide thus obtained was given to mice by gastric gavage. Result showedthat the polysaccharide can marked1y lower the blood sugar and liver glycogen of mice without affecting bloodlipid level. When given intraperitoneally, it also showed hypoglycemic activity- For alloxan diabetic mice, thepolysaccharide can also markedly lower its b1ood sugar by gastric gavage.

Objective To understand psychological pressure of nurses in wards of retired cadres,eatablish incentive mechanism accordingly and further improve nursing quality. Methods Incentive mechanism was applied to nurses in wards of retired cadres to settle their psychological pressure. Results After application of incentive mechanism,14 nurses and 1 nursing worker showed placid psychological state,keep forging ahead actively.Nursing quality of the wards increased. Conclusion Application of incentive mechanism can effectively alleviate psychological pressure of nurses.

Objective To evaluate the clinical efficacy and safety of the cefazedone sodium in treatment of respiratory tract bacterial infections. Methods Single-blind randomized control trial was performed to evaluate the efficacy and safety of cefazedone sodium in treatment of respiratory tract bacterial infections, and cefazolin sodium as the controlled one. Patients in trial group (using cefazedone sodium, 35 patients) and control group (using cefazolin sodium, 37 patients) were all intravenously administered at the same doses of 2 g, every 12 hours for 5 to 14 days. A total of 72 patients were enrolled in the studies, 34 in trial group and 32 in control group, with 1 excluded and 5 withdrawn. Safety assessment was conducted in 71 patients. Results In trial group and control group, the total cure rates for respiratory tract infections were 91.18% (31/34) and 78.12%(25/32), and the general bacterial eradication rates were 80.00% (20/25) and 84.00% (21/25). The adverse drug reaction rates were 22.86 %(8/35) and 16.67% (6/36),there was no significant difference (P > 0.05 ). Conclusion Cefazedone sodium is a safe and effective antibiotic in the treatment of acute respiratory tract bacterial infections.

Objective To study the laparoscopic uterine artery occlusion effect of assisted myomectomy for ovarian function.Methods 90 cases of existing operation indications ofuterine leiomyoma patients were selected as the research objects and divided into 45 cases of the control group and 45 cases of the study group.The control group received laparoscopic uterine myomectomy,the study group received laparoscopic uterine artery occlusion assisted laparoscopic myomectomy comparison between the two groups,the curative effects of the patients with operation and influence on ovarian function and sexual function.Results In the control group,the operation time (72.03 ± 13.53) min was longer than that of the study group(60.05 ± 10.32) min(t =4.722,P ＜0.05);control group,amount of bleeding during the operation was (125.43 ± 17.69) mL,more than that of the study group (82.46 ± 13.89) mL (t =12.816,P ＜ 0.05);the two group of exhaust time after operation (t =0.711,P ＞ 0.05) the time of hospitalization and postoperative (t =0.466,P ＞ 0.05) showed no significant differences.The control group after 3 months of follicle stimulating hormone (FSH),estradiol (E2) were (6.08 ± 1.35) U/L,(80.31 ± 5.36) pmol/L,the study group after 3 months of FSH,E2 were (6.85 ± 1.42) U/L,(72.39 ± 5.35) pmol/L,the difference between the two groups was statistically significant(t =2.636,t =7.015,P ＜0.05);sexual function in patients of the two groups after scores were significantly higher than those before operation,there were no statistically significant differences between the two groups (P ＞ 0.05).Conclusion Laparoscopic uterine artery blocking effects of laparoscopic assisted myomectomy in patients with ovarian function can recover quickly,safely and reliably.

Objective To evaluate the changes of left atrial(LA)function in peripartum cardiomyopathy(PPCM)patients using two?dimensional speckle tracking echocardiography(2DSTE). Methods Totally 35 PPCM patients and 35 healthy postpartum women(control group)were en?rolled in this study. Left ventricular end?diastolic diameter(LVEDd)and LA anteroposterior dimension(LAAD)were measured. The end?diastol?ic volume(EDV)and end?systolic volume(ESV)were obtained using biplane modified Simpson′s method. Cardiac output(CO)and left ventricu?lar ejection fraction(LVEF)were also calculated. E wave and A wave of mitral valve were measured,and correspondingly E/A ratio were obtained. LA longitudinal systolic strain(SS),systolic strain rate(s?SR),early diastolic strain rate(e?SR),and late diastolic strain rate(a?SR)were ob?tained by 2DSTE. Results There was no statistical difference of E wave between the two groups(P>0.05). Compared to the normal control group, LVEDd,EDV,ESV,LAAD,E/A were increased,while CO,LVEF,A,SS,s?SR,e?SR,a?SR were decreased in the PPCM group(P<0.05). a?SR was positively correlated with A wave in patients with PPCM(r=0.775,P=0.001). Conclusion LA reservoir,conduit and booster pump func?tion were decreased during PPCM. 2DSTE can easily and accurately assess these changes of LA function.

BACKGROUND:Previous studies have suggested that achaete-scute homology 1 (ASCL1) plays a key role in the neuronal commitment. Therefore, somatic cels may directly differentiate into neurons by gene transfection ofASCL1, which wil provide new therapeutic strategies for optic nerve regeneration. OBJECTIVE:To construct a recombinant adenovirus vector expressing ratASCL1 gene for further research ofASCL1 gene function. METHODS:The ratASCL1 gene and advenovirus shuttle plasmid (pYr-adshuttle-4) which contained enhanced green fluorescent protein (EGFP) reporter gene were cleaved by restriction endonucleaseXhoI andEcoR I. The target gene fragments were connected together to generate a recombinant plasmid pYr-ads-4-rat-ASCL1 and then transfected into E.coliDH5α. The plasmid was confirmed to be constructed as expectation by enzyme digestion and sequence reaction. The plasmid pYr-ads-4-rat-ASCL1 and pAd/PL-DEST were reconstructed by homologous recombination processes to obtain rat ASCL1 recombinant adenovirus vector. The plasmid pYrAd-rASCL1 was linearized byPac I and subsequently transfected into HEK293 cels for packaging and amplification. RatASCL1 gene in the recombinant adenoviruses were identified by PCR. Virus titer was determined by tissue culture infectious dose 50. Infection efficiency was monitored by EGFP expression. RESULTS AND CONCLUSION:Restriction endonuclease digestion and DNA sequencing showed that the recombinant adenovirus vector pAd-rat-ASCL1 was constructed correctly. The positive amplification bands of 862 bp could be seen in PCR analysis. The virus titer reached 2×1010 pfu/mL. Infection efficiency of recombinant adenovirus in HEK293 cels was more than 80%. The results indicate that the recombinant the adenovirus vector containingASCL1 with high titer and infection efficiency has been successfuly constructed, which can be helpful for further research of the function and clinical application ofASCL1 gene for optic nerve regeneration.