Objective To analyze the effect of cyclooxygenase-2（COX-2）on the invasion and metastasis of gastric cancer with Helicobacter pylori（H.pylori）infection.Methods A total of 60 gastric cancer specimens were included in this study.H.pylori infection was deter-mined by improved Giemsa method and the expression of COX-2 was examined by immunohistochemistry.The correlation between H.pylori infection and the clinical pathological features of gastric cancer was analyzed.Results H.pylori infection was detectable in 36 of 60 cases（60.0%）,and correlated with the staging（χ2=28.040,P =0.000）and lymph node metastasis（χ2=25.482,P =0.000）,but not with the age,sex of patients or histological types.H.pylori infection also significantly linked to the expression of COX-2（χ2=24.326,P 〈 0.001）.Con-clusion H.pylori infection may promote the invasion and metastasis of gastric cancer by increasing the expression of COX-2.

Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.

Objective To investigate the incidence of the aspirin resistance in secondary prevention of cerebral infarction, and the relationship between the aspirin resistance and the cerebral infarction recurrence or other vascular events during the follow-up periods.Methods Aspirin were taken from the first day of admission in 600 patients with cerebral infarction.The platelet aggregation rate was measured after 7-10 days to screen the patients with aspirin resistance or aspirin sensitivity.All patients were followed up for 6 to 24 months and the cerebral infarction recurrence and other vascular events were recorded.Logistic regression model was used to estimate the risk factors of aspirin resistance, vascular events and prognosis.Results Of 600 patients, 150 (25.0% ) patients were resistant to aspirin and 450 (75.0% ) patients were sensitive to aspirin.The proportion of female and diabetes patients, and the level of low density lipoproteins (LDL) in the aspirin resistance group were higher than those in the aspirin sensitivity group.Diabetes (OR = 2.58, 95% CI 1.37-4.85, P=0.003) and high LDL level (OR = 1.89, 95% CI 1.21-2.93, P = 0.005 ) were independent risk factors of aspirin resistance.The incidence of cerebral infarction recurrence and myocardial infarction and all-cause mortality in the aspirin resistance group were all higher than those in the aspirin sensitivity group.Diabetes ( OR = 2.47, 95% CI 1.36-4.65, P = 0.003 ) , atherothrombosis cerebral infarction (OR = 2.13, 95% CI 1.24-3.95, P = 0.023) and aspirin resistance (OR = 3.86,95% CI 1.79-5.87, P = 0.002) were independent risk factors of vascular events during the following-up period.In the patients with aspirin resistance, the risk of the recurrence of vascular events increased 3.86 times.Conclusions The incidence of aspirin resistance is high in secondary prevention of cerebral infarction.Aspirin resistance is closely correlated with cerebral infarction recurrence and other vascular events.

Humans ; Vertigo ; diagnosis ; physiopathology ; Vestibular Function Tests

Analysis of medical project is the first step in the work of medical institutions to carry out medical project cost accounting. It introduces a case of a general hospital by analyzing the structure, department distribution, contact ratio of medical project to design the practical steps of medical project cost accounting.

Objective To explore the change of liver mitochondrial calcium metabolism and its effect on mitochondrial respiratory function.Methods The model of dog biliary obstruction was made by bile duct ligation. Mitochondria were obtained by differential centfifugation.Mitochondrial calcium content,calcium uptake rate and respiratory function were observed and compared.Results Liver mitochondrial calcium content was increased sig- nificantly,calcium uptake rate and respiratory function were obviously reduced after biliary obstruction.Mitochon- drial calcium content was highly negatively correlated with mitochondrial respiratory function (P

Objective To determine serum soluble interleukin 2 receptor level in patients with posthepatitis B liver cirrhosis.Methods Serum sIL 2R was measured using enzyme linked immunosorbent assay.Results Serum sIL 2R was significantly higher in patients with posthepatitis B cirrhosis than that in controls(P

Objective We selected 3 268 genes of human myocardial cells and analyzed the variability of mRNA expression of myocardial cells in normal adults,to explore the variation of energy metabolism related genes in human myocardial cell and compared the characteristics and variation tendency of energy metabolism related genes in human myocardial cells in the early stage.Methods Microarray was prepared with 3 368 myocardial cell related cDNAs.And we studied differentially expressed genes between myocardial cells and multi-tissues in normal adults by PCR,sequence,fluorescent probe labelling and double hybridisation.Results Functional group analysis was carried out with 3 268 genes of myocardial cells in normal adults.Compared with Universal Human Reference,576 genes from myocardial cells were up- regulated(≥1.5 folds).Expression of 1 199 genes were down-regulated(≤66.7%).194 genes were involved in cell energy metabolism.Among them,17 genes were up-regulated and 4 genes were down- regulated.65 genes were related with cellular skeleton,18 of them had up-regulation and one of them had down-regulation.Coneluslon Compared with multi-tissues,there were many genes preferentially expressed in myocardial cells of normal adults,which can be used as a reference system to evaluate the different stages and different pathological behaviors of cardiovascular diseases.

Objective To elucidate the relationship between liver mitochondria calcium content and respi- ratory function in obstructive jaundice and biliary drainage.Methods The model of obstructive jaundice and bil- iary drainage was produced in dogs by common bile duct ligation and choledochoduodenostomy.The liver mitochon- dria were isolated and the calcium content and respiratory control rate were monitored.Results Mitochondria res- piratory control rate and calcium content significantly decreased and elevated respectively after bile duct ligation and there were varying degrees of recovery after biliary drainage,and the degree of recovery was evidently correlated with the duration of obstruction before drainage and the time after drainage.There was a strong negative correlation between the mitochondria calcium content and change of respiratory function.Conclusion The mitochondria cal- cium overload may be one of important factors for suppressed mitochondrial respiration in obstructive jaundice and early relief of the obstruction could favour the recovery of damaged liver mitochondria.

Molecular imaging is a newly rising branches of science which comes from molecular biology technology and modern medical imaging,and it would be the development tendency of mendiacl imaging. This article briefly introduces the basal principle of molecular imaging,the difference and correlation between molecular imaging and traditional medical imaging and the development prospect of medical imaging.