Adrenergic alpha-Antagonists ; therapeutic use ; Cholinesterase Inhibitors ; therapeutic use ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Glomerulonephritis, IGA ; diagnosis ; drug therapy ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

Identification of the histotype of the Wilms' tumor(WT) before operation, application of normative staging rules for WT and individualized combined therapy schedule are the main studying contents of WT' s diagnosis and therapy. Present article will review the overseas and domestic advancements of WT' s diagnosis and therapy, and provide some references for domestic doctors.

Objective:To investigate the immuno-effects triggered by dendritic cells (DCs) pulsed with Heat-treated lymphoma cells.Methods:Mononuclear cells from healthy human peripheral blood(PBMC) were isolated and DCs were generated in vitro with normal methods.Lymphoma cells were heated (42℃,2 h) and then loaded onto DCs.Immunotypes of DCs and lymphocytes were detected with flow-cytometric analysis.IFN-γ releasing was detected by ELISPOT,lymphocyte cytotoxicity was analyzed by MTT method after mixing with DC reaction.Results:After pulsing DCs with hyperthermia lymphoma cells,CD80~+,CD86~+ and HLA-DR existed higher than those of the control group,IFN-γ releasing and cytotoxicities were also strengthened,but there was no difference between them,cytotoxitic lymphocytes (CTLs) were produced more as CD8~+,CD25~+ and CD56~+ cells by flow-cytometric analysis.Conclusion:Anti-lymphoma immuno-effects could be strengthened by loading DC with hyperthermia lymphoma cells.

Objective To study the erythrocytic morphological changes in unwashed autologous drainage blood after total knee arthroplasty (TKA), on account of the postoperative anemia status that was poorly improved after reinfusion. Methods 20 patients (34 knees) underwent TKA from November 2000 to March 2001. There were 6 males and 14 females, whose average age was 66 years (range, 32-79 years). TKA was performed unilaterally in 6 patients, and bilaterally in 14 patients. The disorders were diagnosed as OA in 17, RA in 2 and AS in 1 respectively. All of them received unwashed autologous drainage blood reinfusion, using CBCⅡ Consta VacTM blood salvage system. Blood routine tests were performed preoperatively and on the second and seventh day postoperatively. Blood routine and blood smear tests were also performed on the preoperative blood samples, the postoperative venous blood and drainage blood samples at the first and sixth hour respectively. Erythrocytic morphological changes in these samples were observed under light and electric microscope. Results In the unilateral TKA group, the blood loss in the operation was 208.3 ml, the mean volume of drainage blood was 910 ml,of which 708.3 ml was reinfused; One patient received 800 ml of allogenic bank blood due to postoperative anemia. In the bilateral TKA group, the blood loss, the mean volume of the drainage blood and reinfused volume of blood were 296.4 ml, 1718.2 ml and 1370 ml respectively; Five patients received a total amount of 2200 ml of allogenic bank blood, the average volume was 440 ml. Anemia of different degrees was found at the 2nd and 7th day postoperatively. Compared to venous blood, the levels of hemoglobin and hematocrit were lower in shed blood, and more erythrocytes were deformed. Conclusion The relatively large number of deformed erythrocytes is one of the important reasons that the anemia status is poorly improved postoperatively after reinfusion.

AIM To observe the anti hyperglycemic effect of sodium orthovanadate on type 2 diabetes rats. METHODS Rats were fed by highly fatted food to grow into obesity. Then values of plasma free fatty acid (FFA) were tested. Glucose disposal was measured with the hyperinsulinemic euglycemic clamp technique to assess insulin resistance in vivo. Small dose of streptozocin was injected ip with insulin resistance. Those with plasma glucose over 11 1 mmol?L -1 were assigned to type 2 diabetes model group, continually fed with sodium orthovanadate for 3 days and assayed the values of plasma glucose in fasting state. RESULTS ①Rats fed with fatted food needed more insulin to maintain normal plasma glucose, ie (0 54?0 02) U?min -1 , higher than that of normal control ( P

Objective To study the adverse effects on periodontal tissues caused by crownless roots in elderly people with osteoporosis. Methods Totally 60 12?week SD rats(30 male and 30 female)were randomly divided into the control group(10 male and 10 female sham?operated rats)and the osteoporosis group(20 male and 20 female castrated rats). After 12 weeks,the bone mineral density of right femora was measured by dual energy X?ray for all rats to confirm osteoporosis. The right molar crown was pinched off with haemostatic forceps and the molar root was retained. The 10 con?trol rats and the 20 experimental rats were sacrificed respectively at postoperative 2 and 4 weeks. The periodontal tissues of right molar were sampled and the morphology of the parodontium and the alveolar bone was observed by haematoxylin and eosin(HE)staining and the expression of tumour necrosis factor?alpha(TNF?α)and inducible nitric oxide synthase?2(NOS2)was detected by immunohistochemical staining and real time fluores?cent quantitative PCR(qRT?PCR). Results The HE slices indicated that the periodontal tissues in the osteoporosis group were significantly im?paired compared to the control group as the structure of parodontium loosened. Bone impairment aggravated over time. TNF?αstaining showed as a few claybank spots on the parodontium in the control group,while the staining colour was darkened in the osteoporosis group. The expression of TNF?αmRNA was significantly higher in the osteoporosis group than in the control group(P<0.05). The result of NOS2 was contrary to that of TNF?α. The differences in TNF?αand NOS2 were not statistically significant at 2 and 4 weeks(P>0.05). Conclusion The crownless roots in rats with os?teoporosis cause adverse effects of periodontal tissues as the roots accelerate absorption of the surrounding alveolar tissues. It is indicated that crown?less roots in elderly people with osteoporosis should be removed as soon as possible.

Objective To understand the mental status of 180 stomatology graduates(including undergraduates,masters and PhD students)in China Medical University,whom are hunting job in 2016. Methods A total of 180 stomatology graduates were tested by SCL?90 symptom check?list and self?questionnaire. Results Compared with the national norm,4 factors of SCL?90 in stomatology graduates shows significant differences (P<0.05),they were obsessive?compulsive symptoms,depression,anxiety and paranoid. Chi?square test showed that the 4 factors of masters and doctoral graduate students were significantly higher than undergraduates(P<0.05),and there was no difference between the former two(P>0.05). Multivariate logistic regression analysis showed that family economic conditions were positively correlated with postgraduate examinations;their will?ing to work in stomatology hospitals were closely related to anxiety,depression and other negative emotions. Statistical techniques with univariate re?gression analyses indicated that households’financial status,whether to study for graduate student and doctoral student further,job object for stoma?tological hospital,dental clinic,or department of stomatology in general hospital may be risk factors. Conclusion There are various degrees of men?tal health problems in stomatology graduates of 2016. We should actively guide them,and strengthen their mental health education.

Objective To investigate the state of oxidative s tr ess in patients with different glucose tolerance and the relationship between ox idative stress and insulin resistance(IR).Methods The levels of serum superoxide dismutase (SOD) and malo ndiadehyde(MDA)were measured in subjects of controls,obesity,impaired glucose to lerance (IGT) and newly diagnosed type 2 diabetes mellitus(T2DM).Results Oxidative stress has already existed in obesity and IGT group.MDA level was negatively correlated with insulin sensitive index (ISI) an d SOD level was positively correlated with ISI(all P

Chinese materia medica (CMM) has the great economic value in modern society, which depends on its industrialization. And the development of industrialization needs the protection of intellectual property rights. This paper built the evaluation index system of intellectual property of CMM including 6 first class indicators, 15 second class indicators, and 18 third class indicators. Then we used the standard deviation method to determine the weight of each evaluation index and evaluated the intellectual property of CMM from 2004 to 2013 in China by weighted average method. The results showed that, the intellectual property of CMM was in a steady growth trend in 2004-2013. Finally, the countermeasures and suggestions have been put forward from the government, enterprises, and research staffs.

Abstract: Objective　To evaluate the effects of sunitinib on Japanese encephalitis virus (JEV) infection in vitro and vivo. Methods　The 4-week-old BALB/c mice infected with JEV by intraperitoneal injection. The infected mice were treated with sunitinib for 5 days and 10 days respectively. After that, the change of weight and survival rate were evaluated continuously. The viral load variation in mice brain were detected by qRT-PCR. Indirect immunohistochemical staining assay (IFA) was used to detect the number and distribution of CD4+/CD8+T cells in mouse brain. IFA was also used to observe the expression of virus E protein in the brain of mice. Vero cells were infected with JEV in vitro and given a certain concentration of sunitinib to observe the cell survival status. The expression of virus E protein in cells was detected by IFA. Results　Continuous administration of sunitinib significantly improved the survival rate of infected mice. Survival rate and body weight changes showed that the 5-day's administration strategy was significantly better than the 10-day's administration strategy. The treatment of sunitinib decreased the infiltration of CD4+/CD8+T cells in the brain and reduced the changes of vascular sleeve. However, the variation of viral load and E protein expression in brain were not obvious. The cytopathic effect (CPE) of infected Vero cells were slightly relieved after giving sunitinib, and the expression of E protein was also slightly changed. Conclusion　Sunitinib can significantly reduce the mortality of infected mice, and the 5-day's administration strategy is significantly better than the 10-day's administration strategy. Sunitinib decrease T lymphocyte infiltration in brain of mice, relieve the encephalitis symptoms ,and prolonged the life of mice.