Objective To construct 7 shRNA plasmids targeted to Gcn5 and to explore their role on histone acetylation in regulating MSCs differentiation into cardiomyocytes.Methods MSCs were isolated and passaged.Seven shRNA plasmids were constructed by pGenesil-1(4.9 kb) vector with GFP gene.MSCs were treated with 10 ?mol/L 5-azacytidine respectively for 24 h,2,4 and 7 d and transfected with the plasmids via lipofectamine~(TM)2000.Gcn5,as the marker of the acetylation,and GATA4,as the marker of cardiomyocytes,were detected in MSCs during differentiation with or without shRNA plasmids transfection by RT-PCR.Results MSCs were fibroblast-like but changed morphologically after induction.Seven Gcn5 shRNA plasmids were constructed and identified by restriction endonucleases PstⅠ/SalⅠand DNA sequencing.The expression of Gcn5 and GATA4 could be detected in MSCs without shRNA plasmids tranfection,but no expression after shRNA plasmids transfection.Conclusion Gcn5 plays a very important role in MSCs differentiation into cardiomyocytes,which lays the basis for further research of the importance of acetylation in the regulation of cell differentiation.

It is a mature technology producing single-use syringes by polypropylene(PP). But the traditional sterilization process for single-use syringe pollutes the environment, and the residues endanger the health of users. Irradiation sterilization, instead of traditional sterilization process, is the developing trend. However, the traditional PP does not have the ability of radiation resistance. These methods can improve the radiation resistance ability of PP effectively by adding the stabilizer or floating agent. Toughening or temperature treatment can have the same function. Radiation-resistant PP is introduced in this paper. Furthermore, the developing trends for the materials are discussed.

? AIM: To investigate the immunosuppression of artemisinin in the early-phase response( EPR) of allergic conjunctivitis in a murine model induced by pollen.?METHODS:Twenty-five Balb/c mice were divided into five groups at random: A: model group, B: artemisinin topical treatment group, C: oral artemisinin group, D:dexamethasone topical treatment group, E: negative control group. Balb/c mice were first sensitized with mixture of ragweed crude extract and complete Freund’s adjuvant by left footpad and root of tails injection at days 0. At days 7 and 14, mice were strengthen sensitized by intraperitoneal injection. Negative control group replacd with a mixture of equal amounts of Freund’s adjuvant and PBS mixture. Treatment time was between 21-27d:group B received 1% topical artemisinin eye drops 4 times per day; group C received artemisinin at doses of 600mg/kg orally once per day; group D received topical dexamethasone eye drops 4 times per day. The model and naive groups replaced with solvent for control. After treatment, mice were excited by ragweed pollen crude infusion drops. Within 1h after the excitation, the eyes were taken for histopathology testing, the serum for detection of specific IgE for ragweed pollen.?RESULTS:After excitation, allergy symptoms of model group were more significantly compared with negative control group and the treatment groups; allergy symptom score, mast cell degranulation ratios and the ragweed pollen specific IgE in serum in the topical treatment groups and oral group were higher than that in the negative control group, but significantly lower than that in model group.?CONCLUSION: Artemisinin topical treatment and oral treatment for mice could inhibit the symptoms of pollen allergic conjunctivitis, early-phase response of mast cell degranulation ratio and specific IgE generation for ragweed pollen, suggesting that artemisinin has some therapeutic effect for pollen allergic conjunctivitis.

Objective To observe the evaluation of multi‐tumor marker protein chip in breast cancer with neoadjuvant chemo‐therapy ,and to find high saitivity tumor makers ,then evaluate the clinical significance .Methods Forty health examination persons (control group) and sixty‐four breast cancer patients(experimental group) were accepted with multi‐tumor marker protein chip in‐spection ,then we evaluated the markers of diagnosis .The experimental group patients were accepted four cycles preoperative chem‐otherapy with TAC plan ,and tested multi‐tumor marker protein chip three weeks after therapy .Then we evaluated the clinical sig‐nificance of the markers above‐mentioned .Results The positive rate of CA153 ,CA125 ,CEA and FT was 32% ,27% ,21% and 19% in the experimental group ,it indicated significant change compared with the control group (P<0 .05) .And it indicated signifi‐cant reducing change of CA153 ,CA125 and CEA in the experimental group (P<0 .05) after therapy .The sensitivity would be in‐creased when the four markers were tested combined .Conclusion CA153 ,CA125 ,CEA and FT could be used as assistance diagno‐sis index ;CA153 ,CA125and CEA could keep consistence to the curative effect of neoadjuvant chemotherapy and have clinical value .

Objective To screen the optimized processing technology of wine radix Scutellariae .Methods With the the com‐prehensive score of the yield rates of baicalin ,wogonoside and baicalein as the index ,the orthogonal design was used to investigate the mass fractions of yellow wine quantity ,stir‐frying temperature and frying time of wine radix Scutellariae .Results The optimum yellow wine mass fraction was 10% ,the stir‐frying temperature was 120℃ ,the frying time was 6 min ,and this processing technolo‐gy was stable and feasible .Conclusion The orthogonal design can be used for the optimization of processing technology of wine ra‐dix Scutellariae .

Synthetic agonists of peroxisome proliferator-activated receptors-?(PPAR?) have an important role in the preclinical models of metabolic diseases.In the recent years,it has been demonstrated that PPAR? agonists can normalize blood lipids and reduce insulin resistance and adiposity in rodents and primates.PPAR? activation in the skeletal muscle regulates the oxidative capacity of the mitochondrial apparatus,switches fuel preference from glucose to fatty acids and reduces triacylglycerol storage.PPAR? agonists also reduce insulin levels in serum,increase the sensitivity of insulin and improve glucose-tolerance.The genetic polymorphism in PPAR? has been shown to be associated with blood lipids,obesity and type 2 diabetes.

Stem cell is a kind of cells characterized by self-renewal and multi-differentiation potential;its committed differentiation is the basis of development,growth and renewal in organisms.Recently interest in controlling the differentiation of stem cells is rapidly increasing.The common modulating factors in stem cell differentiation include transcription factors,growth factors and intercellular interactions mediated by membrane proteins.It is shown that the expression level of transcription factors may have important influence on the control,maintenance and recovery of stem cell properties.The latter two factors,growth factors and intercellular interactions,may play a key role in modulating the directional differentiation of stem cells exogenously.This review mainly introduces current research status about those modulating factors in stem cell differentiation.

To direct at the weak points in traditional teaching,teaching methods have been reformed in human parasitology.This article discusses practice in teaching reform of human parasitology.

Objective　To evaluate the effects of the excimer laser in situ keratomileusis (LASIK) for treatment of high myopia.Methods　LASIK was performed on 308 eyes of 186 patients who were followed up for one year. The patients were divided into two groups (group A and group B) according to preoperative diopters.Results　One year after surgery, in group A(-6.0～-10.0D) the uncorrected visual acuities of 0.5 and 1.0 were in 95.76% and 77.58% of the eyes, the mean residual refraction was -0.32D±0.46D. In group B(-10.25～-20.0D) the uncorrected visual acuities of 0.5 and 1.0 were in 62.24% and 37.06% of the eyes, the mean residual refraction was -0.92D±1.13D.Conclusion　It is an effective and safe refraction surgery to correct high myopia with LASIK.

The imported schistosomiasis cases in the Chinese literature and reports from the infectious disease monitoring in-formation system from 1979 to 2013 were collected and compiled. Totally 365 cases were reported to be infected with outside schis-tosomiasis, including 239 cases of schistosomiasis haematobia (74.0%) and 71 cases of schistosomiasis mansoni (22.0%), and 42 foreigners (11.5%) and 323 Chinese (88.5%). The infection areas involved 15 countries and regions in Africa. Totally 74.92%of pa-tients worked in the construction or geological prospecting in the wild field. According to the analysis of these cases, we found that the management system of prevention and control of imported schistosomiasis cases was not perfect including the high missing re-port rate, high misdiagnosis rate, and no standard diagnostic criteria. We suggest that all the levels of CDC or health administrative authorities should adopt the following relevant control measures to strengthen the imported schistosomiasis prevention and control in order to reduce the damage to the public health and the risk of the spread of African schistosomiasis in China:1. to establish and perfect the imported schistosomiasis monitoring and control system in China;2. to integrate the dynamic information platform of la-bor export and establish the comprehensive prevention and control management system of infectious diseases;3. to standardize the diagnosis and treatment of oversea imported schistosomiasis; 4. to strengthen the research on the transmission risk of imported schistosomiasis in the territory of China.