Objective To investigate the relationship between gastroesophageal flap valve (GEFV) and reflux esophagitis(RE).Methods We analyzed 239 cases of RE diagnosed by endoscopy in our hospital from January 2007 to December 2009.RE was classified by Los Angeles classification system and GEFV by Hill system.The association of GEFV with RE was analyzed.Results The overall incidence of abnormal GEFV was32.9% (316/960) and that of RE was 24.9% (239/960).The incidence of severe RE ( grade C and D) in abnormal GEFV group was significantly higher than that in normal GEFV group (23.1% vs.2.4% , P = 0.000).RE grades were positively correlated with GEFV grades (r = 0.308, P = 0.000 ).The incidence of abnormal GEFV in RE patients was higher than that in non-RE (65.3% vs.22.2% ,P = 0.00 ).In terms of gender, there were more males than females in both RE group (63.6% vs.36.4% ) and abnormal GEFV group (60.1% vs.39.9% ).Additionally, incidence of RE increased with age, i.e.positively correlated with age (r=0.214,P =0.000).The incidence of abnormal GEFV also increased significantly in those over 30 years old, i.e.positively correlated ( r = 0.129, P = 0.000).Conclusion There is correlation between GEFV abnormality and RE.GEFV provides useful information for assessment and prediction of the reflux status of the patients.

Objective: To study correlation among serum levels of lipoprotein a[Lp(a)], uric acid (UA) and cardiac function in patients with acute myocardial infarction (AMI) complicated diabetes mellitus (DM).Methods: A total of 70 AMI+DM patients were enrolled as AMI+DM group, 60 pure AMI patients were enrolled as pure AMI group, 60 pure DM patients were enrolled as pure DM group, and 60 healthy people were selected as healthy control group.According to NYHA cardiac function class, AMI+DM patients were divided into class I~II group (n=58) and class III~IV group (n=12);according to their prognosis, they were divided into no cardiovascular event group (n=54) and cardiovascular event group (n=16).Serum Lp(a) and UA levels were measured in all group, and their correlation were analyzed.Results: Serum Lp(a) and UA levels of AMI+DM group were significantly higher than those of healthy control group, pure AMI group and pure DM group, and those of pure AMI group and pure DM group were significantly higher than those of healthy control group, P<0.01 all;Pearson compared with class I~II group, there were significant rise in serum levels of Lp(a)[(183.28±19.96) mg/L vs.(335.65±22.71) mg/L]、UA[(287.45±21.82) μmol/L vs.(437.79±26.72) μmol/L]in class III~IV group (P<0.01 both);compared with no cardiovascular event group, there were significant rise in serum levels of Lp(a)[(220.75±25.72) mg/L vs.(423.97±30.81) mg/L]and UA[(308.49±21.82) μmol/L vs.(442.97±29.75)μmol/L]in cardiovascular event group, P<0.01 both;Pearson correlation analysis indicated that serum Lp(a), UA levels were significant positively correlated with cardiac function class (r=0.410, 0.412, P<0.05 both), and serum Lp(a) level was significant positively correlated with UA level (r=0.496, P=0.023) in AMI+DM patients.Conclusion: Serum Lp(a), UA levels are closely correlated with cardiac function in AMI+DM patients, which can be used as indexes diagnosing and monitoring disease progress.

Although hemopoietic stem cell transplant have been used in treating malignant diseases of hematological system using high-dose chemotherapy and radiotherapy before transplant or non-transplant is very important. When the dose of chemotherapy and radiotherapy exceeds the ability the tissues can bear, normal tissue can be badly damaged, which limits the therapeutic efficacy of chemotherapy and radiotherapy. Treatments of hematological malignancies are expected to achieve the best therapeutic efficacy and the least damage of normal cells and tissues. Amifostine is an inorganic thiophosphate eytoprotective agent,its chemname is S-2(3-aminopropyl) amino dihydrogen phosphate.h is a prodrug whose active constitutent is free thiol,it can clears free radicals produced from cytotoxic drugs and radiotherapy in tissues exposed to them. It is a broad-spectrum cytoprotective agent which can protect multifarious normal tissues against damage of chemotherapy and radiotherapy. Using amifostine in proconditioning of hemopoietic stem cell transplant can lessen hematological and renal toxicity, it can also lessen renal toxicity produced by chemotherapeutics as platinum,cyclophosphamide and vincristine. The main purpose of this review is to discuss the cytoprotection of amifostine in hematological malignancies.

Objective: To study the effects of mechanical strain on the proliferation of human osteoblasts. Methods: Cloned MG 63 human osteoblasts were cultured and seeded at 10 5/well in 6 well Bioflex cell cuture plates. Subsequently, the cells were exposed to mechanical strain stimulation (at 6%, 12% and 24% elongation rate respectively) for 24 hours using Flexercell 3000 Cell Stretching Unit. MTT colorimetric method was used to assess the proliferation of the cells. Results: The proliferation of MG 63 cells was significantly increased 24 h after mechanical strain treatment( P

Objective To observe the clinical effect of patients in the department of neurosurgeryoperation combined with autologous blood transfusion. Methods A retrospective analysis of 113 cases of operation patients in department of neurosurgery. All the patients were under general anesthesia using autologous blood transfusion. Thepreoperative , the first dayand the third day postoperative blood routine indexes , coagulation function examination and the electrolyte were analyzed. Resuits Theblood transfusion volume was (262.53 ± 163.41)mL. Hemoglobin , hematocrit and platelet count after 1 days and 3 days after operation were significantly lower than preoperative. The potassium ionsthat first day postoperation was significantly higher than preoperation , postoperative 1 day and the 3 day after the operation of sodium ions and chloride ions were significantly increased. Conclusion Patients in the department of neurosurgery of autologous blood transfusion is safe and effective, which can save the blood and reduce the risk of allogeneic blood transfusion.

OBJECTIVE:To prepare hydroxycamptothecin sustained-release tablets and to optimize its preparation technology and formulation.METHODS:The preparation technology and formulation were optimized by orthogonal experiment taking the type of excipient polylactic acid (A),tabletting pressure (B),ratio of HCPT to PLA (C) as factors with the deviation degree of the accumulative release percentage as evaluation index.Then the optimized results were verified.RESULTS:The optimal technology and formulation were as follows:A was PLA (20 000),B was 250 N,C was 1∶5.The deviation degree of the formulation was verified to be 2.963.CONCLUSION:The hydroxycamptothecin sustained-release tablets prepared in optimized preparation technology and formulation are up to the standard on preparation.

About half of the world population and more than 90% of rural households in the developing countries still use unprocessed biomass fuels (wood, dung and crop residues) as the fuels. These substances, burning in the open fire or in the simple stoves, can cause indoor air pollution and the pollutants exposure in women and children is more serious. Biomass smoke contains lots of toxic and harmful substances which may impact the health of human. This article presented a recent research progress in the indoor air pollution and health effects caused by combustion of biomass fuels in China and in the world.

OBJECTIVE: To investigate the inhibitory effect of hydroxycamptothecin (HCPT) on C6 gliomas cells in rats. METHODS: The cytotoxicity of HCPT at different concentration(1～0.031 25 mg?mL-1) on C6 in vitro was measured by MTT with conventional therapy for C6 gliomas cells as control. Meanwhile,the antitumor effect of O-HCPT(open ring form) and that of C-HCPT(closed ring form) were compared. RESULTS: At a high concentration,HCPT showed an inhibition ratio of as high as(69.43?4.5)% on C6 gliomas cells in concentration-dependent manner,showing no significant differences as compared with control. The antitumor effect of O-HCPT was lower than that of C-HCPT,and their IC50 were 3.626 mg?mL-1 and 0.135 mg?mL-1,respectively. CONCLUSION: HCPT had remarkable inhibitory effect on C6,and the antitumor effect of C-HCPT is potent than that of O-HCPT.

OBJECTIVE:To provide some suggestions for reference in establishment of clinical pharmacist institution in China.METHODS:Through analysing the present policy,mechanism,education,social psychology and law,the difficulties confronting us in establishment of clinical pharmacist institution were discussed.RESULTS&CONCLUSION:It needs to improve the mechanism in many aspects and to intensify the reform continuously in the process of establishing clinical phar-macist institution.

OBJECTIVE:To study the characteristics of doctors'behavior in clinical pharmaceutical service in hospi?tal.METHODS:Using statistical method,the doctors at their posts in nationwide scope were sampled and investigated by question-naires.RESULTS&CONCLUSION:Hospital doctors have a relatively vague cognizance of clinical pharmacists'work and they are cautious about clinical pharmaceutical service.They admit that they have requirements of clinical pharma?ceutical service,but at the same time they doubt of the pharmacists'capability,and they are just willing to accept the service selectively.