Objective To explore the factors associated with infections in children with leukemia after chem-otherapy. Methods Clinical data of fifty-six children with leukemia, who infected after chemotherapy were reviewed with infection characteristics and some related factors. Results The infection rates were 27% in upper airway,37% in lower upper airway,12% in oral cavity,5% in gastrointestinal tract,3% in perianal abscess,7% in urinary sys-tem,5% in scpficaemia and 2% in ofitis media. Most infection happened from five to ten days after chemotherapy,and the infection rates varied with chemotherapy scheme. The persistence time of infection were (8.5±2.2) days in the children with the numbers of neutrophil below 0.5×109/L and (3.5±1.4) days in those above 0.5×109/L with significant difference(P <0.01). Conclusions After chemotherapy,the children had more chances to be infected. To be more exact, the /nfection was more common observed in respiratory tract, next in gastrointestinal tract. And the stronger the medicine used the number of heterophil granulocyte dropped much more ,the infection happened more easily.

Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Objective To investigate the status of personality, family function and psychological resilience towards employed nurses, and to explore the relationship among personality, family function and psychological resilience. Methods Four instruments were applied to investigate 240 employed nurses from comprehensive Grade 3A hospital of Fujian Province, which were the Characteristics Questionnaire, Family Intimacy Scale (FACEⅡ-CV), Self-resilience Scale and Personality Traits Questionnaire. Results The correlation analysis showed that nurses′ personality traits, family functions and psychological resilience were closely related. The multiple regression and analysis showed that 40%of the variance for employed nurses′psychological resilience could be explained through considering personality dimensions, family cohesion and adaptability. Conclusions Personality, family functions and psychological resilience towards employed nurses are closely related as both internal and external protective factors.

Objective To investigate the suppressive effects of Maytansine on the proliferation,migration and invasion of nasopharyngeal carcinoma (NPC) cells.Methods MTT assay was employed to study the effect of Maytansine on the viability of 5-8F and SUNE1 cell lines.Wound healing assays and transwell were used to assess the effects of Maytansine on the migration and invasion capacities of NPC cells.Furthermore,an integrated bioinformatics analysis was conducted to clarify the potential mechanisms of Maytansine.Results Time course analysis of Maytansine treated NPC cells indicated significant decrease in cell viability,assessed by MTT.Furthermore,we identified that Maytansine reduced the migration and invasion capacities of NPC cells.Moreover,bioinformatics analysis showed that Maytansine suppressed the proliferation,migration and invasion via the upregulation of CDKN1B,BMP2,HBEGF,SMAD6,ADM,VEGFA,CTGF and IL6 in NPC cells.Conclusion Maytansine plays a significant suppressive role in the proliferation,migration and invasion of NPC cells and may be explored further for its potential therapeutic effects on NPC in humans.

Objective To study the efficacy as well as side effects of the Nimotop in the treatment of benign memory disorders with oral tab. Method 38 patients with benign memory disorders as treatment group, taking Nimotop oral tab 30mg, Tid for 6 weeks and anathr 34patients with benign memory disorders taking Pyrrolidone Acetamide oral tab 0. 8, Tid as control group. Result The total efficiency were 73.6% in treatment group, while 47% in control group. Although some improvement in the memory scale and memory quotient were found in both groups, but the treatment group is obviously superior to control group ( P ＜ 0. 05) . Conclusion Nimotop has positive effect on the treatment of benign memory disorders.

Objective　To study the mRNA expression of MCT1 and MCT2 genes in human hepatocellular carcinoma (HCC) and paracarcinoma liver tissue (PCLT). Methods　The semi-quantitative analysis of MCT1 and MCT2 genes mRNA expression in human HCC and PCLT was conducted by RT-PCR method and electrophoresis band opacity density (OD) comparison analysis method in 25 patients with HCC. Results　The mRNA expression of MCT1 was significantly higher than MCT2 in HCC and PCLT, in HCC the mRNA expression of MCT1 and MCT2 genes were significant higher than that in PCLT. Conclusions　The high expression of mRNA of MCT1 and MCT2 genes in HCC indicates that these genes may take a significant role on lactate and other monocarboxylate transmembrane transportation and on pH regulation in tumor cells.

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently. With the enhanced staining efficiency, shortened test duration, simplified operation, increased positive detection rate and accuracy, the new technology lays a foundation of clinical definite, curative effect view and prognosis.

Objective To design an intravenous indwelling needle in battle field aid.Methods Several concavity were made on the hollow tube inner surface posterior puncture needle case,and some corresponding convexity uplifted peripheral needle handle pistons posterior puncture needle core.Revolution could be avoided because the needle handle pistons were just embedded in hollow tube concavity.It was convenient to operate and decreased the operator's orientation transformation by the needle's handhold vane adopting rectangle instead of butterfly shape.Results The improved puncture needle was stable and the rate of success of intravenous puncture was increased.Conclusion The improved venous retaining needle can be applied in battle field aid.