Matrix metalloproteases(MMPs)and tissue inhibitors of matrix metalloproteases(TIMPs) have a co-regulation of changes of the extraeellular matrix(ECM).MMPs can control the proteases which are related to the process of tumor development.Moreover,MMPs,produced by host cells including inflammatory ceUs,endothelial cells,pericytes and fibroblasts have a positive or negative correlation with tumor formation.

Inflammatory bowel disease(IBD) is characterized by chronic non-specific inflammatory dis ease.The inflammation caused by abnormal immune response in intestinal mucosa exerts an important role in the pathogenesis of IBD.T helper 17 cells(Th17)are involved in the development of chronic inflammation and auto immune disease,whereas regulatory T cells can inhibit the function of autoimmune,and thus the balance of Th17/Treg balance plays an important role in intestinal homeostasis.Various strategies can be done to modulate Th17/Treg balance,such as antigen-presenting cells,microoganisms,some key molecules,natural compounds,proving a promising therapeutic method for IBD.

Climacteric syndrome is a common clinical disease with high incidence.The understanding of the disease is progressing in recent years.This article collects thirty-seven documents in this field in recent eight years.The pathogenesis of climacteric syndrome is divided into two aspects as the deficiency of the kidney and the imbalance of the liver, the spleen and the heart.It also sums up the therapy of climacteric syndrome from this two aspects.At last, the authors propose that we should pay attention to the importance of the liver, the spleen and the heart in both the pathogenesis and the therapy.

Inflammatory bowel disease is a chronic non-specific intestinal tract inflammation,currently,its etiology and pathogenesis are not clear.The current conventional treatments include salicylic acid drugs, steroids, immunosuppressive agents, biological agents and surgery, but the prognosis is still not very optimistic.In recent years, a variety of other treatment options have emerged, such as anti-cytokine antibody, anti-chemokine antibodies.Clinicians pay more attention to treatment of inflammatory bowel disease.

Objective To give a systematic reviews on effect of taking blood gas via cephalic artery for infants.Methods Meta-analysis were used to analyze literatures about taking blood gas via cephalic artery for infants.Results 11 studies were included.The rate of successful puncture was 87.5％～98.0％ in the intervention group and 55.3％ ～94.6％ in the control group.The rate of successful puncture via cephalic artery were higher.Conclusions Effect of taking blood gas via cephalic artery for infant was better than that via other artery.Successful rate was high,while the incidence rate of local side effects was low.

Objective]To discuss and analyze Professor Cheng Jing's clinical experience for treating decline in ovarian reserve(DOR). [Method]Through learning from the tutor in the outpatient service and case studies ,we analyzed and summarized Pro. Cheng's recognition on the etiology and pathogenesis of DOR,together with his dialectical way of thinking, combined therapy of Chinese and western medicine ,and empirical prescriptions. [Result] Pro. Cheng believes that this disease lies in the kidneys as wel as in the thoroughfare and conception meridian ,the pathogenesis is kidney deficiency and the imbalance of the thoroughfare and conception meridian,or combined with the stagnation of liver qi,spleen deficiency,and blood stasis and phlegm fire (heat); the therapy is mainly nourishing and warming kidneys as wel as conditioning the thoroughfare and conception meridian ,combined with Chinese medicine's periodical therapy,such as adjusting the circulation of qi and blood in livers,spleens,hearts and other organs. [Conclusions] Pro. Cheng's combined therapy of Chinese and western medicine for curing DOR ,of which his empirical prescriptions the Bushenyichongkangshuaitang is the main part,is of remarkable curative effect and significant clinical value.

Objective:To investigate and analyze of informed consent in assisted reproductive technology ( ART) institutions, understand the status and problems.Improve the content and methods of informed consent. Methods:A questionnaire was investigated for the medical staffs and patients anonymously in ART institutions. Results:The implementation of informed consent need to be improved.Medical staffs protect the patient privacy inadequately.The patients also not pay attention to the informed consent.Medical staffs look forward to ethics training.Conclusions:The current situation of informed consent is not optimistic in ART institutions.The ethics training need to be strengthen to improve the awareness of medical staffs about informed consent.The content and methods of informed consent should be standardized,and explore the evaluation methods to enhance the effect of in-formed consent.

Objective To investigate the expression of miR-199b in colorectal cancer and the effect of miR-199b on metastasis of colorectal cancer.Methods The expression of miR-199b in colorectal cancer was assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR).The miR-199boverexpressed human colon tumor-116 (HCT-116) stable cell lines was constructed by lentivirus assay.The effects of miR-199b on the migration and invasion of colorectal cancer were confirmed by Transwell assay.Bioinformatics,luciferase reporter assay,and enzyme linked immunosorbent assay (ELISA) were performed to verify the potential target genes of miR-199b.Results miR-199b was at lower level in cancerous tissues comparing to the corresponding noncancerous tissues.Up-regulation of miR-199b significantly blocked the migration and invasion of colorectal cancer cells.Co-transfection of miR-199b and VEGFA wild type (WT)3'untranslated region (3'UTR) could inhibit the luciferase activity,while there was no significant difference when co-transfection with mutant (MT) 3'UTR.The protein level of vascular endothelial growth factor A (VEGFA) after overexpression of miR-199b was repressed,which was validated by ELISA.Conclusions miR-199b was a new colorectal cancer suppressor miRNA,which inhibited tumor migration and invasion by negatively regulating VEGF.

Angioplasty, Balloon, Coronary ; Humans

Primary intestinal lymphangiectasia is a rare disease, which is characterized by the obstruction of lymphatic return, dilated lymphatics and distorted structure of villi. Lymph fluid leaks into the small bowel lumen as a result of the obstruction of lymphatics and elevated pressure of lymphatics, and leads to malabsorption and loss of proteins. The causes and pathogenesis of PIL are not clear, hut the abnormal immunologic mechanism has got people's attention. Recently, the endoscopic technique has been improved, and the diagnosis rate of PIL has increased obviously. Antisecosis is the basic and effective treatment, especially for children.