Objective To explore the value on application of combined C-reactive protein(CRP), serum albumin(PA)with blood routine inspection in diagnosis of bacterial infectious diseases in children.Methods 98 cases of patients confirmed infectious diseases in our hospital were selected as the study objects, and 68 cases with bacterial infectious diseases were as the bacterial infection group, while 30 cases with non-bacterial infection were as the non-bacterial infection group.Another 50 cases of healthy children were as the control group.All the children and children in the control group were given CRP, PA and blood routine detection, The diagnostic coincidence rates were evakuated according to the examination Results .ResultsCRP and WBC levels in the bacterial infection group were significantly higher than those in the non-bacterial infection group and the control group (P<0.05), PA level in the bacterial infection group were significantly higher than that in the non-bacterial infection group and the control group (P<0.05), The children's urine and cerebrospinal fluid or sputum bacterial culture results as the gold standard, 68 cases were true positive, and 30 were negative.CRP diagnosis showed 40 cases were positive, 58 cases negative, 13 cases false positive, and 41 cases false negative, the detection sensitivity was 39.71%, specificity was 56.67%, and coincidence rate was 44.90%.PA diagnosis showed 43 cases were positive, 55 cases negative, 12 cases false positive, and 37 cases false negative, the detection sensitivity was 45.59%, specificity was 60.00%, and coincidence rate was 50.00%.WBC diagnosis showed 47 cases were positive, 51 cases negative, 14 cases false positive, and 35 cases false negative, the detection sensitivity was 48.53%, specificity was 53.33%, and coincidence rate was 52.04%.CRP, PA, WBC combined diagnosis showed 62 cases were positive, 36 cases negative, 3 cases false positive, and 9 cases false negative, the detection sensitivity was 86.76%, specificity was 90.00%, and coincidence rate was 87.76%.The sensitivity, specificity and coincidence rate of CRP, PA and WBC combined diagnosis were significantly higher than those of CRP, PA and WBC diagnosis results.There were no significant differences in sensitivity, specificity and coincidence rate among single diagnosis of CRP, PA and WBC.Conclusion Implementation of combined CRP, PA with WBC in diagnosis of bacterial infection in children can effectively improve diagnostic coincidence rate.and provide theoretical basis for clinical treatment.

Objective To investigate the effect of diabetes mellitus on neuromuscular blocking effect of cisatracurium in patients. Methods Forty ASA Ⅱ patients, aged 30-64 yr, weighing 44-90 kg, scheduled for neurosurgical operation under general anesthesia, were assigned into 2 groups (n = 20 each): type 2 diabetes mellitus group (group D) and non-diabetes mellitus group (group ND). General anesthesia was induced with midazolam,fentanyl and etomidate. Neuromuscular block was assessed with Epoch XP nerve electrophysiology monitor. A train-of-four stimulation of ulnar nerve was used. Cisatracurium 0.15 mg/kg was injected intravenously over 5 s after T1 was maintained at 100%. Tracheal intubation was performed after the onset of the muscle relaxant. Anesthesia was maintained with iv infusion of propofol 4-8 mg· kg - 1 · h - 1 and remifentanil 0.1-0.3μg· kg - 1 · min - 1. The effect of inmbation was evaluated and graded. The onset time, clinical duration, recovery time and recovery index were recorded. Results The onset time was significantly longer in group D than in group ND ( P ＜ 0.05). There was no significant difference in the clinical duration, recovery time and recovery index and intubation effect grade between the two groups ( P ＞ 0.05). Conclusion Diabetes can prolong the onset time of cisatracurium, but has no effect on the clinical duration and recovery time.

[Objective] To observe the effects of modified Zhengrong Decoction combined with acupuncture for acquired paralytic strabismus (APS). [ Methods ] Forty - one cases of APS were treated with modified Zhengrong Decoction combined with acupuncture of Jingming (BL1) point mainly. [Results] Four (9.8%) cases were cured after one treatment course (10 days), 23 (56.1%) cured in 2 ~ 3 treatment courses and after 4 ~ 6 treatment courses 12 (29.3%) cured, 1 (2.4%) ineffective, and 1 (2.4%) relapsed, the total effective rate being 95.2% . [Conclusion] Modified Zhengrong Decoction combined with acupuncture is effective and safe for APS.

Objective:To evaluate the effects of human dental pulp stem cells-conditioned medium(HDPSCs-CM)on the osteogenic differentiation of human dental follicle stem cells(HDFSCs) in vitro.Methods:HDFSCs were in vitro cultured,purified and identified.CCK-8 assay was applied to evaluate the HDFSCs viability after 7 days cultured by HDPSCs-CM;the morphological changes of HDFSCs were observed;the osteogenic differentiation was studied by alkaline phosphatase(ALP) staining and alizarin red staining.The mRNA expression of POSTN,Col-Ⅰ,ALP,BSP and OPN was detected by Real-Time PCR.Results:Induced by HDPSCs-CM,HDFSCs exhibited several characteristics of cementoblast or osteoblast lineages.In the induction group the viability of HDFSCs was inhibited(P<0.05 or P<0.01),ALP staining was stronger and there were more mineralized nodules,the expression levels of POSTN,Col-Ⅰ,ALP,BSP and OPN were upregulated(P<0.05 or P<0.01).Conclusion:HDPSCs-CM can promote the osteogenic differentiation of HDFSCs.

ObjectiveTo observe the effects of catalpol, the effective component of Rehmanniae Radix, on atrionector cell apoptosis andβ-tubulin in vitro rabbit injured by simulated ischemia reperfusion;To explore the mechanism of treating sick sinus syndrome.MethodsAtrionector cells were collected from newborn rabbits. Cells were divided into 5 groups:normal group, model group, catalpol high, medium and low dose groups. Anoxia and aglycaemia were established to simulate ischemia. Atrionector cellular damage models were established by recovering the supply of oxygen and sugar. Normal control group and model group were given the same volume of culture medium, while catalpol high, medium and low dose groups were given medicine with relevant concentrations (100, 20, 10μg/mL, respectively). ELISA, FCM, laser scanning confocal microscopy were used to observe apoptosis rate andβ-tubulin of atrionector in each group.Results Apoptosis rate of the model group was obviously higher than the normal group (P<0.01), andβ-tubulin cleavage was obvious. Apoptosis rate in catalpol high, medium and low dose groups were significantly lower than that of the model group (P<0.01);β-tubulin structure were significantly more complete compared with the model group;the fluorescence intensity was significantly higher than that of model group (P<0.01).Conclusion Catalpol can inhibit atrionector cellular apoptosis caused by simulated ischemia reperfusion. Its protective effects on atrionectorβ-tubulin may be the mechanism of the treatment of Rehmanniae Radix for sick sinus syndrome.

Objective To observe the effects of ferulic acid on cell apoptosis andβ-tubulinin vitro of rabbit sinoatrial node injured by hypoxia and low glucose, and to explore its mechanism.Methods Model cells were established on sinoatrial node cells from newborn rabbit by performing deprivation of oxygen and glucose. Then the model cells were divided into 5 groups: a normal control group, a model group, ferulic acid high, medium and low dose group. The normal control group and the model group were treated with equal volume of culture medium, and the three volume ferulic acid groups were treated with ferulic acid of 100, 20, and 10μg/ml respectively. Flow cytometry, laser scanning confocal microscopy were used to observe the sinoatrial node apoptosis rate and cytoskeleton proteinβ-tubulin in each group.Results Apoptosis rate of the model group was obviously higher than the normal group (56.95% ± 11.99%vs. 31.45% ± 6.32%,P<0.01), whileβ-tubulin cleavage was significantly lower than the normal group (5.141 ± 0.218vs. 8.035 ± 0.762,P<0.01). Apoptosis rate of ferulic acid high, medium dose group were significantly lower than the model group (24.85% ± 6.34%, 26.70% ± 9.84%vs. 56.95% ± 11.99%,P<0.01),β-tubulin structure were significantly more complete compared with the model group (7.927 ± 0.357, 5.961 ± 0.351vs. 5.141 ± 0.218,P<0.01).Conclusions Ferulic acid can suppress apoptosis of sinoatrial node cells caused by low glucose and oxygen. Protecting the sinoatrial node cell skeleton protein ofβ-tubulin may be one of the mechanisms.

To adapt to changes in hospital positioning and management incurred by such health reforms as the “Global budget of medical insurance”and “Separation of clinic from pharmacy”,the performance evaluation system needs to be optimized constantly.Such information technology as big data collection,storage and analysis can help the system in supporting the success of the health reform. Combination of medical big data and performance management can guide the hospital toward goals of the reform,and to achieve all-win.

Objective To evaluate the role of transient receptor potential ankyrin 1 (TRPA1) in the dorsal root ganglion neurons in the development of diabetic neuropathic pain (DNP) in rats.Methods Twenty-four Sprague-Dawley rats with DNP were randomly divided into 3 groups (n-=8 each) using a random number table:DNP group,TRPA1-specific siRNA group (siRNA group) and TRPA1-negative siRNA group (NC group).Another 8 Sprague-Dawley rats with normal blood glucose served as control group (C group).In siRNA group,TRPA1-specific siRNA 45 μl was injected intrathecally.In NC group,TRPA1-negative siRNA 45 μl was injected intrathecally.In DNP and C groups,normal saline 45 μl was injected intrathecally.On 2nd day after intrathecal administration,the lumbar segment (L4-6) of the dorsal root ganglions was removed for determination of the expression of TRPA1 mRNA.On 7,14,21 and 28 days after intrathecal administration (T1-4),MWT was measured.Results Compared with DNP group,TRPA1 mRNA expression was down-regulated in siRNA and C groups.Compared with DNP group,and MWT was significantly decreased at T1.2 in siRNA group,MWT was decreased at T1-3 in NC group,MWT was increased at T1-4 in group C.Compared with siRNA group,MWT was significantly increased at T1-4 in group C.MWT was significantly higher at T1~ in group C than in NC group.Conclusion TRPA1 in the dorsal root ganglion neurons is involved in the development of DNP in rats.

Objective To know the effects of living with the elderly on nursing students' willingness engaging in aged nursing and attitude towards the elderly so as to provide references for nurturing professionals for caring for the aged.Methods 231 nursing students were surveyed.The questionnaire included four parts,general information,life experience with the elderly,willingness engaging in aged nursing,attitude towards the elderly and cognition of aging.Results 45.9 percent of the nursing students were willing to engage in aged nursing.Living with elderly and relationship with the elderly affected their willingness.Conclusions Creating more opportunities for nursing students in touch with the elderly can help nursing students to have a good impression of the elderly and stimulate them to choose aged nursing.

Objective To explore the effect of different states of the environment to the rehabilitation process and complications of preterm infants in the different environment in neonatal intensive care unit (NICU). Methods 120 premature infants were divided into observation group and control group with 60 cases in each group by random digital table method. The control group was treated with routine nursing model, while observation group was treated with bird nest nursing, lower noise and using cyclic lights. Oxygen time and hospitalization time, increasing about body weight, sleeping time on the twenty-eighth days and the incidence of complications between the two groups were compared. Results The hospitalization and oxygen time were (41.79±2.51)days and (11.45±5.22)days respectively in the observation group, those were shorter obviously than that in the control group [(45.32±3.85)days and(15.52±5.89)days, t=5.95, 4.01, P<0.01].The growth of body weight was (17.36±2.94) g/d, the sleeping time on the twenty-eighth day was (21.18 ±0.39)hours in the observation group. It was increased than that in the control group [(13.48±4.63)g/d and(19.56±0.45)hours], and the difference was significant(t=5.48, 21.07, P<0.01). The incidences of nosocomial infection and feeding intolerance in the observation group were reduced significantly, the difference was significant ( X2=7.06, 8.71, P<0.01). Conclusions Improving the environment in NICU can prevent the adverse factors on premature infants, promote the recovery and growth of premature infants.