Objective:To investigate the effects of salidroside on the proliferation,activation,Collagen Ⅰ secretion as well as expression of ROCK-Ⅰand TIMP-1 in HSC-T6 cell,and to elucidate the molecular mechanism of salidroside against hepatic fibrosis with Y-27632 as the positive control. Methods: HSC-T6 cell line was employed as the model for following study. MTT colorimetric assay was used to detect the proliferation of HSC-T6 cell. ELISA was employed to value the content of collagen Ⅰ in supernatant of HSC-T6 cells treated with salidroside and Y-27632. The effects of salidroside and Y-27632 on expressions of smooth muscle actin (?-SMA)、ROCK-Ⅰand TIMP-1 expression were analyzed by Real-time PCR and Western blot. Results(:1)Both Salidroside(3、6、12 mmol/L)and Y-27632(5、 10、20 ?mol/L)had dose-dependent inhibitory effects on ?-SMA expression which represents the activated state of HSC-T6(P

Objective An experimental study was undertaken to observe the effects of DFMO on the growth and apoptosis in bladder carcinoma EJ cells. Methods The methods of cell culture, living camera technique, eosin dying cell counting and DNA end labeling in situ were used to observe the effects of DFMO on the growth and apoptosis in bladder carcinoma EJ cells. Results Living camera technique showed that cell growth was significantly inhibited after five days of 4 mmol/L, 6 mmol/L, 8 mmol/L of DFMO were administrated compared with that of control group, and this variation was obvious with DFMO dosage increasing. The same was observed with eosin dying cell counting. Tunel method showed that apoptosis was induced by DFMO with 4 mmol/L, 6 mmol/L, and 8 mmol/L, but 6 mmol/L seems to be a suitable dosage. All these methods showed that if exogenous putrescence was added simultaneously, there was no difference significantly compared with that of control group both in cell proliferation and apoptosis. Conclusion The proliferation of bladder carcinoma EJ cell could be inhibited by DFMO and also apoptosis could be induced by DFMO.

Objective To understand the status quo of AIDS discrimination among Xinjiang medical college students and its influence factors .Methods By using the stratified cluster sampling method ,583 students in a Xinjiang medical college were conduc‐ted the AIDS related questionnaire investigation .Results If medical undergraduate students found that the schoolmates carried HIV ,18 .01% of college students would not directly contact with them ,45 .97% of medical college students would not give sympa‐thy and concern ,31 .22% of students would not keep normal communication with them ,53 .17% of medical college students thought that they should drop out of school to go home ,32 .76% of medical college students would not keep a secret to the outside world ;40 .99% of the medical college students would advise them to restrain their own behaviors ,24 .87% of medical college students would choose to report it to the teacher or leader .By the multivariate analysis of covariance ,gender ,nationality ,family monthly in‐come ,origin of students ,AIDS related knowledge ,etc .were the factors affecting the level of AIDS discrimination .Conclusion The medical college students have a certain degree of discrimination to the AIDS patients ,learning the AIDS related knowledge should be strengthened for reducing the fear psychology on AIDS ,thus to eliminate the discrimination towards AIDS .

Objective To acquire effective clues for identification of environmental risk factors to birth defects. Methods Spatial autocorrelation statistics Moran's I and spatial hotspots detect method Getis's G statistics was used to identify birth defect risk factor. Results The different kinds of birth defects have different spatial distribution. Neural tube birth defects have the properties of spatial autocorrelation and different clustering phenomena in different spatial scales. Two typical spatial patterns were discovered in spatial scales of about 6.84 kilometers and 22.8 kilometers. Conclusion Using spatial autocorrelation probing technique, we find that there may be some common environmental teratogenetic factors which affect birth defects occurring ratio in the study area. By hotspots analysis of the clustering phenomena, the risk factors leading to birth defect were further resolved.

Objective To explore the influence of etiquette training on self efficacy and self confidence in nursing freshmen.Methods 183 nursing freshmen were given 36 class hours of etiquette training from Nursing College of Taishan Medical University.They took general self-efficacy scale (GSES),academic self-efficacy scale (ASES) and self-esteem scale(SES) tests respectively before and after the training.Results Compared with before training((2.32±0.22),(58.34±5.68),respectively),the scores of general self-efficacy and academic self-efficacy ((2.48±0.24),(66.32±6.35),respectively) were significantly increased (P＜ 0.01).The scores of self confidence increased significantly after etiquette training ((27.45 ± 2.32),(26.15 ± 2.26),(P ＜ 0.01)).Conclusion The etiquette training can improve the self-efficacy and self confidence of nursing college freshmen.

Objective The aim of this paper was to develop the Mobile Nutrition Order System based on the technology of hospital information system to adapt the nutrition and food security management model in the new situation, so that the unified informa-tization management of diet for patients could be implemented by the Department of Nutrition. Methods The Mobile Nutrition Order System, with the functions of information entry, nutrition recipe, order making and data transmission, could be constructed by the com-bination of technologies between informatization and digital order. Results The automation of nutrition order in hospital was a-chieved, and the nutrition recipe and nutritional therapy could be realized in hospital, the effect was very obvious. Conclusion Since the application of Mobile Nutrition Order System, the labor intensity of servers has been reduced, saving working hours effective-ly.Meanwhile, the management of nutritional therapy was improved and the quality of logistics services in hospital and nutritional ther-apy ascend to a new high.

Objective To investigate the effects of antisense phosphothioate oligodeoxynucleotides (AS-ODNs) W086 on drug-resistant gene CTX-M expression in Escherichia coli producing extended-spectrum β-lactamases(ESBLs).Methods AS-ODNs liposome was introduced into the purpose bacteria B052.The total colony forming unit(CFU) was counted.The bacteria growth curve was drawn by microplate reader.The inhibition effects of AS-ODNs on the expressions of drug-resistant gene CTX-M were observed by RT-PCR in B052.The minimal inhibitory concentration(MIC)was determined by fluid dilution method.Results significant growth inhibition of cells treated with W086 was observed as compared with those in cells in control treated bacteria.The number of B052 colonies significantly decreased in all W086 treated groups in a concentration dependent manner (P ＜ 0.05),while CFU of B052 was not influenced in simple liposome group,simple W086 group and controlled chain group.The expression of CTX-M was selectively inhibited.Conclusion Efficiently and specificly blocking expression of CTX-M mRNA,AS-ODNs reverses the multiple drug resistance of B052.It indicates that AS-ODNs provides a new viable strategy to reverse antibiotic resistance problem.

To explore the clinical value of contrast-enhanced ultrasound (CEUS) in differentiating benign and malignant focal liver lesions (FLLs) with SonoVue, CEUS was used to examine 113 patients with focal liver lesions (FLLs) in our hospital during July 2005 to December 2006. All the patients underwent contrast-enhanced CT (CECT) or contrast-enhanced MRI (CEMRI). Except for patients with focal fatty sparings (n=18) and with hemangiomas (n=8), all the patients were confirmed by operation or ultrasonic-guided liver puncture biopsy. A sulfur hexafluoride gas-based contrast agent was used with a MI of 0.15 to 0.17. Forty-eight cases of malignant FLLs, including 30 hepatocellular carcinomas (HCCs), 2 cholangiocarcinomas and 16 metastatic tumors, were detected. Seventy-eight cases of benign FLLs, including 33 hemangiomas, 9 focal nodular hyperplasias (FNHs), 19 focal fatty sparings, 5 abscesses, 7 regenerative nodules and 2 inflammatory pseudo-tumor, were involved. The contrast pattern of benign and malignant FLLs was quite different. CEUS has higher specificity and sensitivity than conventional ultrasound in differentiating benign and malignant FLLs.
Carcinoma, Hepatocellular/*ultrasonography ; Contrast Media/*diagnostic use ; Diagnosis, Differential ; Hemangioma/ultrastructure ; Image Enhancement/*methods ; Liver Diseases/*ultrasonography ; Liver Neoplasms/*ultrastructure ; Magnetic Resonance Imaging ; Sensitivity and Specificity ; Young Adult

Objective To evaluate the effectiveness of locking compression plate (LCP) in treatment of comminuted fractures of distal end of radius. Methods A retrospective analysis of the clinical data was done for the 24 patients with comminuted fracture of distal end of radius who had been treated by locking compression plate fixation from August 2002 to August 2003. Results The follow up duration averaged 7.8 months. The satisfactory rate of functional outcome with LCP fixation was 91.6 percent. Conclusion Although LCP can provide the outstanding stability theoretically, it has not resulted in more exciting outcomes in treatment of comminuted fractures of distal end of radius than traditional methods.

Objective To observe the temperature-time curve of Shenque(CV8) salt moxibustion and explore the temperature starting mechanism of Shenque moxibustion and itsclinical significance.Method In the subjects treated with Shenque salt moxibustion, periumbilical and umbilical bottom temperatures were measured simultaneously and recorded using a two-channel temperature logger, and the temperature-time curve was drawn and analyzed to preliminarily explore its temperature starting mechanism according to the experimental results.Result Periumbilical temperaturemeasurement showed that the temperature that every cone of moxa produced reached more than 43℃and the maximum temperature reached 44.9℃. The time that 43℃or more lasted was (4.50±2.36) min. The time that 40℃or more lasted was (8.03±2.63) min. Umbilicalbottom temperature measurement showed that the temperature reached more than 43℃at the seventh cone of moxa on average and the maximum temperature reached 44.7℃. The mean value of the maximum temperatures at the seventh to tenth cones of moxa was (43.44±1.05)℃. The time that 43℃or more lasted was (5.53±2.13) min. The time that 40℃or more lasted was(18.44±10.39) min.Conclusion Shenque salt moxibustion has the property of repeated warm stimulations, which can avoid the platform period brought about by stable warm stimulation and promote the maximization of moxibustion intensity to effectively increase clinical moxibustion quantity finally.