1.Effect of Salmeterol and Fluticasone Propionate Combined with Conventional Therapy on Airway Remode-ling and Bone Metabolism in Patients with Severe Chronic Obstructive Pulmonary Disease
China Pharmacist 2016;19(3):548-551
Objective:To observe the effects of salmeterol and fluticasone combined with the conventional treatment on the airway remodeling and bone metabolism in the patients with severe chronic obstructive lung disease ( chronic obstructive pulmonary disease, COPD. Methods:Totally 90 patients with severe COPD were divided into the observation group and the control group according to the order of admission. The control group was received the conventional treatment, and the observation group was treated with salmeterol and fluticasone additionally. The course of treatment was 8 weeks. Before the treatment and in the 3rd and 12th month after the treat-ment, the changes in the levels of bone mineral density ( BMD) in femoral neck bone, serum osteocalcin ( BGP) , alkaline phosphatase (AKP), the total number of induced sputum cells, neutrophils percentage, forced expiratory volume in one second (FEV1), leukocyte mediated element 8 (IL-8), matrix metalloproteinase 9 (MMP-9) and other indicators were detected and compared. Results: BGP, AKP and BMD of the two groups in the 3rd month after the treatment were not significantly changed (P>0. 05), while in the 12th month after the treatment, BMD in the observation group was significantly lower than that in the control group and that before the treat-ment, and BGP and AKP in the observation group were significantly higher than those in the control group and that before the treatment (P<0. 05). The total number of induced sputum cells, the percentage of neutrophils, FEV1, MMP-P and IL-8 in the two groups were significantly improved in the 12th month after the treatment (P<0. 05), and the difference between the two groups was statistically sig-nificant except IL-8 (P<0. 05). Conclusion:Salmeterol and fluticasone propionate can effectively improve airway remodeling in the patients with COPD. Short time use of salmeterol and fluticasone propionate shows no adverse effect on the bone metabolism, while long-term use would lead to reduced bone mineral density.
3.Experimental and clinical study on diagnosis of acute intestinal ischemia in early stage
Shiwen WANG ; Jinchun HE ; Ailin SONG ; Linyan WANG
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo study the diagnostic value of alteration of serum enzyme in acute intestinal ischemia(AII). MethodsChanges of serum levels of CPK, CKMB, LDH, LA, CRP and CO2CP of venous blood of 40 rabbits and 53 patients in different ischemia conditions at different time before and after operation were measured to determine the relationship between the changes and ischemia degree of intestine. ResultsThe serum levels of CPK, CKMB, LDH, LA and CRP increased gradually with the severity of AII and decreased with the improvement of AII, but CO2CP was the reverse.The 6 assay values mentioned above in animals of AII compared with the control group, and in patients in pretreatment compared with the control group, and in reversible intestinal necrosis compared with irreversible intestinal necrosis, and in nonoperation group 1 h after hospitalization compared with reversible intestinal necrosis group 2 h before operation(except CO2CP)all had remarkable difference(P
4.Effects of eye acupuncture therapy on neurological function and brain-derived neurotrophic factor expression in a rat model of cerebral ischemia/reperfusion injury
Huiying LIU ; Pengqin WANG ; Ying BIAN ; Jinchun WANG ; Yinghong WEI
Chinese Journal of Tissue Engineering Research 2016;20(18):2634-2641
BACKGROUND: Eye acupuncture therapy is a technique used to adjust qi-blood circulation, relax muscles and tendons, and activate col aterals by acupuncture at the acupoints around the eye bal s and in the orbital border. This therapy has been widely used in the clinic because it exhibits remarkable therapeutic effects on many ischemic cerebrovascular diseases. However, the precise mechanism behind this therapy remains poorly understood. Neurotrophic factors are a protein family including neurotrophic factors and brain-derived neurotrophic factors that can regulate neuronal survival, development and functioning.
OBJECTIVE: To investigate the effects of eye acupuncture therapy on neurological function and nerve growth factor and brain-derived neurotrophic factor expression in the brain tissue of rat models of cerebral ischemia/reperfusion injury.
METHODS: Fifty-four male Sprague-Dawley rats were randomly divided into three groups with 18 rats per group: sham-operated, model and eye acupuncture therapy groups. Rat models of middle cerebral artery occlusion were established by the intraluminal suture method in the model and eye acupuncture therapy groups. Eye acupuncture was performed at the fol owing acupoints liver area, upper-jiao area, lower-jiao area and kidney area located at the internal orbital margin at 2 hours after cerebral ischemia/reperfusion injury. Rat neurological function was evaluated at 3, 7, and 14 days after injury. Nerve growth factor and brain-derived neurotrophic factor expression in the rat brain tissue was detected using immunohistochemical staining method at 1 and 2 weeks after treatment. Cerebral infarct size was determined using TTC staining at 2 weeks after treatment.
RESULTS AND CONCLUSION: (1) At 1 and 2 weeks after injury, nerve growth factor and brain-derived neurotrophic factor expression was significantly greater in the eye acupuncture therapy group than in the model group (P < 0.05), but nerve growth factor and brain-derived neurotrophic factor expression in the eye acupuncture therapy group was decreased at 2 weeks after injury compared to that at 1 week after injury. (2) At 7 and 14 days after treatment, neurological function scores in the eye acupuncture therapy group were significantly lowered, and there was significant difference between eye acupuncture therapy and model groups (P < 0.05), but they were significantly higher than those in the sham-operated group (P< 0.05). (3) At 2 weeks after treatment, cerebral infarct size was significantly greater in the eye acupuncture therapy and model groups than in the sham-operated group (P < 0.01), and it was significantly smal er in the eye acupuncture therapy group than in the model group (P < 0.05). (4)These results indicate that eye acupuncture therapy shows neuroprotective effects on ischemic cerebral injury by increasing nerve growth factor and brain-derived neurotrophic factor expression, improving neurological function, and reducing cerebral infarct size.
5.Observation of the effect of improved bone plate fixation and bone grafting in the treatment of intra-articular calcaneal fracture
Chuanwen WANG ; Jiuqin HUANG ; Hongqi WANG ; Jinchun SI
Chinese Journal of Primary Medicine and Pharmacy 2014;21(11):1643-1645
Objective To investigate the effect of improved bone plate fixation and bone grafting in the treat ment of intra-articular calcaneal fracture.Methods 80 patients with intra-articular calcaneal fractures were randomly divided into the two groups.The non-bone graft group(47 feet in 40 patients)was given improved bone plate fixation.The bone graft group(50 feet in 40 patients)was given improved bone plate fixation and bone grafting.Results The incidence rate of complication in the non-bone graft group was 25.0% and that in the bone graft group was 19.1%.The difference between the two groups was not significant(x2 =0.21,P > 0.05).Postoperative Bhler's angle and calcaneal height between the two groups had significant differences (t =14.5,16.99,all P < 0.05).The excellent rate of Maryland functionality in non-bone graft group was 66.0%,which was significantly lower than 86.0% in the bone graft group(x2 =5.38,P < 0.05).The average full weight bearing time between the two groups had significant difference (t =11.17,P < 0.05).Bhler's angle after 1 year and forwa rd Bhler's angle loss of the non-bone graft group had significant differences compared with the bone graft group (t =29.76,9.81,all P < 0.05).Conclusion Improved bone plate fixation and bone grafting in the treatment of intra-articular calcaneal fracture can obtain better results and avoid the long-term loss of reduction.
6.Characteristics of familial hyperlipoproteinemia in Lanzhou
Weijuan LIU ; Jinchun HE ; Wei WANG ; Li ZHAO ; Lanxia ZHOU
International Journal of Laboratory Medicine 2014;(18):2437-2438
Objective To investigate familial hyperlipoproteinemia and the features of familial hyperlipoproteinemia in Lanzhou . Methods Data were from previous studies on the subject .Families of hyperlipoproteinemia were screening ,questionnaires were col-lected ,physical examination and laboratory data of family members were also colleted to analysis the characteristics of familial hy-perlipoproteinemia .Results A total of 39 familial hyperlipoproteinemia families were enrolled in the study ,including 280 family members .There were 15 core families ,11 single-parent families ,and 13 orphaned families .There were 6 familial hypercholesterol-emia families ,9 familial hypertriglycerides families ,24 mixed familial hyperlipidemia families .The children of the first generation ac-counted for 63 .2% of the total number of people enrolled in the study ,the father generation accounted for 14 .3% ,the children of the second geration accounted for 22 .5% .Conclusion In the survey ,the most common type of familial hyperlipidemia was mixed familial hyperlipidemia .The father generation was majority .The member of core families was less than incomplete families .
7.Study on liver fibrosis of chronic hepatitis B with ultrasound tissue characterization
Hanying LI ; Changfeng DONG ; Ting HUANG ; Zhigang WANG ; Jinchun LIU
Chinese Journal of Ultrasonography 2003;0(07):-
Objective To study a kind of quantitative diagnosis method with ultrasound tissue characterization on liver fibrosis of the patients with chronic hepatitis B.Methods Measuring the gray scales of the two dimensional ultrasonography of the patients and compare the gray scales with the fibrosis degrees on histopathology of the patients and finding the differences among every two groups.Results The differences of the gray scales compared with the fibrosis degrees on histopathology were meaningful on statistics( F = 34.3, P
8.The practice on the pharmaceutical analysis course in pharmacy college education
Yan PENG ; Zongchun WANG ; Lingli ZHANG ; Jinchun SONG
Chinese Journal of Medical Education Research 2015;14(4):388-390
To realize the object of the pharmacy college education training,some teaching ideas and methods on pharmaceutical analysis course was explored.In the teaching practice,the course hour of pharmaceutical analysis was adjusted to deepen the understanding of the theoretical knowledge;the practice basic skills training was also strengthened and the professional ethics and quality education was taken throughout the course.Besides,emphasis on the systemic college education was put forward,Pharmacy college education not only be targeted in training applied technical persons but also should meet the students' need for continuing education and continue learning development.
9.Expressions and relativity research of Cyclic B1 and Cyclic D1 in severe reflux esophagus, Barrett's esophagus and esophageal adenocarcinoma
Lan WANG ; Jinchun LIU ; Jingguang LIANG ; Haifang ZHANG
Cancer Research and Clinic 2010;22(2):111-114
Objective To explore the role of Cyclin B1 and Cyclin D1 on Barrett esophagus,Barrett's esophagus mixed with atypical dysplasia and esophageal adenocarcinoma.Methods Cyclin B1 and Cyclin D1 were examined with immunohistochemistry.76 esophageal tissues of patients werB collected,including severe reflux esophagitis(RE,25 cases),Barrett esophagus(BE,35 cases),Barrett esophagus mixed with atypical dysplasia(DY,8 cases),esophageal carcinoma(CA,8 cases).Ten cases with normal esophageal mucosa were examined as the control. Results Cyclin B1 and Cyclin D1 were high expression in the specimens of the BE,DY and CA groups and very low expression in the control and RE group.Statistieal difference Was showed(P<0.01).Expression of Cyclin D1 was increasing gradually from the tissues of intestinal metaplasia,atypical dysplasia to adenocarcinoma(50.04 vs 67.94 vs 74.31).There Was significant difference among these three groups(P<0.01).Conclusion Cyclin B1 and Cyclin D1 as markers of tumour development could evaluate the risk from Barrett esophagus to adenocarcinoma.Perhaps it is the earlv event in the development of esophageal carcinoma.
10. Tau protein and alzheimer’s disease
Chinese Journal of Tissue Engineering Research 2020;24(17):2775-2781
BACKGROUND: Deposition of neurofibrillary tangles is closely related to cognitive decline in Alzheimer’s disease, and tau protein is an important component. OBJECTIVE: To investigate the possible pathogenesis of Alzheimer’s disease and the role of tau protein hyperphosphorylation in the progression of Alzheimer’s disease. METHODS: The first author searched for relevant articles published from January 2001 to January 2019 in WanFang, CNKI, Vip, PubMed, and Embase with the key words of “Alzheimer’s disease; tau protein; β-amyloid cascade.” RESULTS AND CONCLUSION: Hyperphosphorylation of tau protein and formation of paired helix filaments are considered to be the basis of neuronal degradation in Alzheimer’s disease. tau protein may not depend on the cascade response triggered by β-amyloid deposition in the pathogenesis of Alzheimer’s disease, and tau protein-associated vaccine immunity has been verified to produce a curative effect in clinical trials. Further exploration on the relationship between tau protein, β-amyloid protein and Alzheimer’s disease is necessary to better understand the pathogenesis of Alzheimer’s disease, and provide a theoretical basis for the development of therapeutic drugs for Alzheimer’s disease.