OBJECTIVE:To study the effects of propofol on invasion of hepatoma carcinoma HepG2 cell. METHODS:MTT method was used to detect the viability of HepG2 cells which were cultured with 0(negative control),1,3 and 10 μg/ml propofol for 48 h. The ability of cell invasion was detect by Tranaswell method. The phosphorylation level of nuclear factor-κB p65(NF-κB p65) and the expression of matrix metalloproteinase 2 (MMP-2),MMP-9,E-cadhherin and Snail were detected by Western blot method. RESULTS:Compared with negative control,1,3,10 μg/ml propofol inhibited the cell viability and invasion,down-regu-lated the expression of Snail and the phosphorylation level of NF-κB p65,and up-regulated the expression of E-cadherin (P<0.01). 3,10μg/ml propofol down-regulated the expression of MMP-2 and MMP-9(P<0.01). Above effects depended on drug con-centration(P<0.01). CONCLUSIONS:Propofol can suppress HepG2 invasion,which might be related to the inhibition of NF-κB/Snail signal pathway.

Objective To discuss comparison of different analgesic solution to the immune status and serum levels of inflammatory cytokines of the patients with breast cancer after surgery.Methods The patients of experimental group and control group were given Dizocine and fentanyl to analgesic respectively perioperative experimental.Then analysis and comparison the immune status and serum levels of the two groups.Results The T lymphocyte subsets ( The level of CD3 +、CD4 +and the ratio CD4 +/CD8 +) and the level fo NK cells of the exprimental group patients intraoperative and postoperative 1 d were significantly higer than the control group.The anti-inflammatory factor in the serum of the experimental group patients intraoperative and postoperative 1d was significantly higer than the control group.The proinflammatory factor in the serum of the experimental group patients intraoperative and postoperative 1 d was significantly lower than the control group.Conclusion Dizocine can significantly improve the serum inflammatory factors of the patients with brest cancer after surgery,and can adjust the patient’s immune status.

Objective To observe the effects of dexmedetomidine on serum TNF-αand IL-6 in neurosurgical patients during perioperative period. Methods 60 cases patients with intracranial aneurysm treated in our hospital in January 2014 to August 2014 were selected as the research objects, and they were randomly divided into two groups with 30 cases in each group.Before induction, the observation group adopted 1μg/kg dexmedetomidine with 10 min infusion, then adopted 0.4μg/( kg? h) infusion of dexmedetomidine, and control group received saline infusion.Serum TNF-αand IL-6 levels in two groups were compared at different time.Results After application of dexmedetomidine, the related indexes of hemodynamics in observation group, mean arterial pressure (MAP) and heart rate (HR) were more stable (P<0.05).Serum levels of TNF-αat the time of after induction, skin incision operation, 1h operation in observation group were (1.32 ±0.35), (1.42 ±0.54), (1.52 ±0.67), (1.57 ±0.63) μg/L, and were lower than those of control group, respectively (P<0.05).Serum levels of IL-6 at the time of after induction, skin incision operation, 1h operation in observation group were (69.64 ±13.54), (72.43 ±16.75), (76.32 ±16.93), (77.42 ±15.75) μg/L and were lower than those of control group, respectively (P<0.05).Conclusion The hemodynamics after application of dexmedetomidine in patients with neurosurgery operation is more stable, and the serum TNF-αand IL-6 are lower, so it contributes to the anti-inflammatory and has important clinical value.

OBJECTIVE To prevent nosocomial infection from catheterization room and to reduce the rate of postoperative infection, and improve quality of catheterization room medical care. METHODS Air, object surfaces, the hands of medical staff, and using disinfectant in catheterization room were put to the test every month. RESULTS Totally 253 specimens were put surveillance over three years, total qualified rate was 99.6%, that of air was 98.6% and of object surfaces was 100%, for the hands of medical staff was 100%, and for using disinfectant was 100%. CONCLUSIONS Enhancing prevention nosocomial infection can effectively reduce the infection rate. Surveying the catheterization room termly is an important measure to prevent and control the nosocomial infection.

OBJECTIVE:To observe the cerebral protective effects of dexmedetomidine in brain aneurysm surgery. METH-ODS:48 patients with intracranial aneurysm were randomly divided into observation group and control group,with 24 cases in each group. Observation group was given dexmedetomidine 1 μg/kg,pump time 10 min,at rate of 0.5 μg/kg;control group re-ceived constant volume of normal saline. Hemodynamic changes,the levels of S100β protein and neuron specific enolase (NSE) were compared between 2 groups. RESULTS:The heart rate and mean arterial blood pressure were more stable in the observation group after medication,with statistical significance(P<0.05). The serum level of S100β protein at the end of operation(T5),6 h after operation(T6),12 h after operation(T7),24 h after operation(T8)were(1.52±0.35)μg/L,(1.69±0.33)μg/L,(1.72±0.42)μg/L,(2.08±0.57)μg/L;the serum level of NSE were(10.9±1.2)ng/L,(13.5±1.5)ng/L,(16.8±2.8)ng/L and(19.7±4.3)ng/L in observation group;those were all lower than in control group,with statistical significance(P<0.05). CONCLUSIONS:Dexme-detomidine for cerebral aneurysm operation is helpful for hemodynamics stability,and result in lower S100β protein and NSE. So it is important for cerebral protection.

Anthracycline-based antitumor drugs are one of the highly effective broad-spectrum anti-cancer drugs that are commonly used clinically. However, anthracyclines have the serious side effect of cumulative dose-related cardiotoxicity. Clinically, intake of myocardial protective drugs is one of the most important strategies for the safe use of anthracyclines in chemotherapy. Numerous cancer patients un-dergoing chemotherapy also require surgery, during which general anesthesia is commonly used. Various studies have shown that propofol and sevoflurane, the most commonly used general anesthetics, present a significant protective effect in myocardial ischemia-reperfusion damage. Therefore, for patients who have just undergone chemotherapy with anthracyclines, anthracycline-induced myocardial injury may occur at the acute or chronic phase of surgery. Consequently, if the effect of anesthetics on the cardiotoxicity of anthracyclines is similar to the protective effect of these drugs for myocardial ischemia-reperfusion damage, this protective effect on cardiotoxicity will have significant impact on the recovery of cardiopulmonary function of patients after surgery. Thus, further studies are needed to establish the effect of anesthetics on the cardiotoxicity of anthracyclines.

Objective To evaluate the ischemic postconditioning on endoplasmic reticulum stress during cerebral ischemia/reperfusion (I/R) in rats.Methods Ninety adult male Sprague-Dawley rats,aged 350-450 g,were randomly divided into 3 groups (n =30 each) using a random number table:sham operation group (S group),I/R group,and ischemic postconditioning group (group P).Focal cerebral I/R was induced by electrocoagulation of left middle cerebral artery and 30 min occlusion of bilateral common carotid arteries followed by reperfusion.In group P,bilateral common carotid arteries were subjected to 3 cycles of 30 s reperfusion and 10 s ischemia at the beginning of reperfusion.At 24 h of reperfusion,neurological deficit was scored,and cerebral infarct size was detected by TTC staining.At 6,12 and 24 h of reperfusion,the expression of glucose-regulated protein 78 (GRP78),C/EBP homologous protein (CHOP) and caspase-12 in the ischemic area were measured (using immunohistochemistry).The neuronal apoptosis in the ischemic area was detected by TUNEL.Results Compared with S group,the neurological deficit score was significantly increased,cerebral infarct size was enlarged,the neuronal apoptosis was increased,and the expression of GRP78,CHOP and caspase-12 was up-regulated in I/R and P groups.The neurological deficit score was significantly lower,cerebral infarct size was smaller,the expression of GRP78 was higher at 12 and 24 h of reperfusion,the neuronal apoptosis was lower at 24 h of reperfusion,and the expression of CHOP and caspase-12 was lower in group P than in group I/R.Concluion Ischemic postconditioning can inhibit neuronal apoptosis mediated by endoplasmic reticulum stress during cerebral I/R,which dose not play a leading role in cerebral protection in rats.

OBJECTIVE To evaluate the germicidal efficacy of phthalaldehyde disinfectant.METHODS Suspension quantitative bactericidal test was used to observe its bactericidal efficacy and stability.RESULTS The temperature was 20-21℃.The results showed that the average killing rates of Escherichia coli,Staphylococcus aureus and Candida albicans exposed to the solution containing 5 664mg/L phthalaldehyde for 1 min were 99.99%,and the(average) killing rates of spores of Bacillus subtilis var.niger exposed to the solution containing 5 664mg/L(phthalaldehyde) for 60 min and 90 min was 99.94% and 100%,respectively.When its stock solution was stored at 56℃ under airtight condition for 2 weeks,the content of phthalaldehyde decreased by 3.27%.CONCLUSIONS The phthalaldehyde disinfectant is a good and stable bactericide.

Objective To compare the efficacy of different does of remifenanil combined with propofol for colonoscopy.Methods Ninety ASA Ⅰ or Ⅱ patients,aged 35-63 yr,weighing 45-72 kg,scheduled for elective painless outpatient colonoscopy,were randomly divided into 3 groups (n =30 each):remifentanil 0.2 μg/kg group (group Ⅰ),remifentanil 0.5 μg/kg group (group lⅡ) and remifentanil 1.0 μg/kg group (group Ⅲ).In groups Ⅰ-Ⅲ,remifentanil 0.2,0.5 and 1.0μg/kg were injected over 60 s,respectively,and then propofol 1.0 mg/kg was injected intravenously for induction.The colonoscope was inserted after loss of eyelash reflex.When body movement appeared during examination,remifentanil 0.1 μg/kg + propofol 0.5 mg/kg,remifentanil 0.25 μg/kg + propofol 0.5 mg/kg,and remifentanil 0.5μg/kg+ propofol 0.5 mg/kg were added in groups Ⅰ,Ⅱ and Ⅲ,respectively.The operation time,induction time,emergence time,examination room discharge time,and body movement,hypotension,bradycardia,hypoxemia and respiratory depression during operation were recorded.Results There was no significant difference in the operation time,induction time,emergence time,and examination room discharge time between the three groups (P ＞ 0.05).Compared with group Ⅰ,the incidence of body movement was significantly decreased,and the incidences of hypotension and respiratory depression were increased in group Ⅱ,and the incidence of body movement was significantly decreased,and the incidences of hypotension,hypoxemia,bradycardia and respiratory depression were increased in group Ⅲ (P ＜ 0.05).The incidences of hypoxemia,bradycardia and respiratory depression were significantly higher in group Ⅲ than in group Ⅱ (P ＜0.05).Conclusion The optimum dose of remifentanil is 0.5 μg/kg when combined with propofol for colonoscopy.

Objective To evaluate the effect of oral tramadol controlled release tablets premedication on the efficacy of anesthesia with target-controlled infusion (TCI)of propofol and remifentanil for painless colonoscopy in elderly patients.Methods Sixty ASA Ⅰ or Ⅱ patients,aged≥ 65 yr,undergoing painless colonoscopy,were randomly divided into 2 groups ( n =30 each): control group (group C) and tramadol group(group T).In group T tramadol controlled-release tablet 100 mg was administered orally at 2 h before operation.Anesthesia was induced and maintained with TCI of propofol and remifentanil.SP,DP,HR and SpO2 were recorded before induction,at the time to reach the ileocecal valve and at the end of operation.The induction time,duration of operation,emergence time,recovery time,adverse events and the amount of propofol and remifentanil consumed were recorded.Results There was no significant difference in hemodynamics parameters,induction time,duration of operation,emergence time,recovery time,adverse events and the amount of propofol and remifentanil consumed between the two groups (P ＞ 0.05 ).Conclusion Oral tramadol controlled release tablets 100 mg premedication can be safely used in painless colonoscopy in elderly patients,however,tramadol can not enhance the efficacy of anesthesia with TCI of propofol and remifentanil.