1.Study on the Determination of Aristolochic Acid by RP-HPLC
Xiaoxi DU ; Yuehua ZHOU ; Jincai LU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(07):-
Objective To optimize the RP-HPLC procedure for the determination of aristolochic acid. Method Different extraction solvents and methods have been screened to get the most efficient way for sample preparation. The influence of different mobile systems on quantitation were compared to choose an approprate mobile system for determination. Besides,the minimum limits of different detecting waves were measured to give the method to detect trace amount of aristolochic acid. Result Refluxing with 70% methanol was better than other ways in sample preparation. Both methanol-1% acetic acid (70∶30) solution and 0.3% ammonium carbonate solution (pH=7.5)-acetonitrile (75∶25) with wavelength of 250 nm and 319 nm can be used for quantitation while 0.3% ammonium carbonate solution (pH=7.5)-acetonitrile (75∶25) with wavelength of 224 nm for trace detection. The minimum detectable amount was 0.02 ng. Conclusion The method established can be applied to determinate aristolochic acid and detect trace amout exactly.
2.Content Determination of Active Component in Epimedium Koreanum Nakai of Different Pharmaceutical Genetic in Liaoning Province
Na SHAN ; Jincai LU ; Jing WANG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(01):-
Objective To determine the content of icariin and flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic in Liaoning Province. Methods Icariin was used as the chemical reference substance to establish HPLC and ultraviolet spectrophotometry methods for content determination of icariin and total flavonoids. Results The content of icariin in leaves was from 0.197% to 0.347%,and in stem was from 0.043% to 0.050%. The content of total flavonids in leaves was from 6.68% to 8.85%,and in stem was from 1.3% to 2.7%. The calibration curves of icariin was in good linearity over the range of 0.3~1.8 ?g (r=0.999 9) and total flavonids was 0.5~3 ?g/mL (r=0.999 9). The average recovery of icariin was 99.43% (n=6,RSD=0.29%) and total flavonids was 100.38% (n=9,RSD= 1.60%). Conclusions The content of flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic has large difference,the content in leaves is far higher than in stem.
3.Analysis of Drugs for Chronic Hepatic Diseases Used in 8 Hospitals of Xinxiang During the Period 2001~2003
Jihai ZHENG ; Guangzhou LU ; Jincai WANG
China Pharmacy 2001;0(11):-
OBJECTIVE:To review the current situation and the trend of development of drugs for chronic hepatic diseases,so as to provide the information for rational use of these drugs METHODS:By using the methods of sequences of total sales volumn and DDDs,the main drugs for chronic hepatic diseases used in 8 hospitals of Xinxiang during the period 2001~2003 were analysed RESULTS & CONCLUSION:The total sales volumn and DDDs of the liver-protective drugs always occupied the front row of drugs for chronic hepatic diseases,and the use of compound glycyrrihizin(Stronger Neo-Minophagen C) is rapidly increasing
4.Alpha-linolenic acid and linoleic acid in extract of Portulaca oleracea L. determined by high-performance liquid chromatography.
Hailiang XIN ; Yinhuan HOU ; Min LI ; Jincai LU ; Changquan LING
Journal of Integrative Medicine 2008;6(11):1174-7
To determine alpha-linolenic acid and linoleic acid in extract of Portulaca oleracea L. by high performance liquid chromatography (HPLC).
5.Relativity analysis between quality of life and sub-health state of guangdong urban dwellers
Huali XU ; Xiang ZHANG ; Yan LU ; Jincai QIU ; Jun XU
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(5):442-444
Objective To explore the relationship between quality of life and sub-health state of Guangdong urban dwellers.Methods A total of 2 200 urban dwellers were investigated using SF-36 and Sub-Heahh Measurement Scale Version 1.0(SHMS V1.0).Results As for the scores in PCS,MCS,psychosocial symptom,cognitive function,social adaptability,those of man urban dwellers (respectively (78.89± 15.25),(73.15 ± 15.26),(63.73± 18.82),(62.13± 19.34),(68.04± 17.90)) were higher than those of woman dwellers (respectively(76.98± 14.20),(71.76± 14.72),(60.53± 18.28),(57.00± 17.89),(65.73± 17.07)).The difference was statistically significant (P<0.05).Bivariate correlation analysis showed that the PCS and MCS of quality of life were positively related with all dimensions of sub-health (P<0.001).Canonical correlation analysis indicated that vitality,social adaptability,psychosocial symptom and positive emotion were the main factors of sub-health status,and MCS was of quality of life.Conclusion Sub-health state of Guangdong urban dwellers is positively related with quality of life,and the more serious sub-health state is,the worse quality of life is.Therefore,intervention of subhealth and quality of life should be targeted to be strengthened according to the investigation.
6.The evaluation of complications after endovenous laser treatment for patients with varicose veins of the lower extremities
Shaoying LU ; Guangyu QI ; Xintian HUANG ; Jincai CHEN ; Jingsen SHI
Chinese Journal of General Surgery 2001;0(09):-
Objective To explore causes of complications after endovenous laser treatment (EVLT) , for patients with superficial varicosities of the lower extremities. Methods In this study, 207 patients with a total of 268 lower limbs were treated by EVLT combined with surgical procedures. According to CEAP classification, there were C2 in 268 limbs, C3 in 56 limbs, C4 in 101, C5 in 23 and C6 in 18. Simple EVLT was performed for 25 limbs in 21 patients; EVLT combined with external banding valvuloplasty of superficial femoral vein for 23 limbs in 23 patients; EVLT combined with ligation of great saphenous vein (CSV) and denudation of superficial varicosities for 220 limbs in 163 patients. Results An average of 10?5 (3-24) months of follow up was achieved in 175 cases. Early complications (within the first 2-weeks after operation) included bruising or ecchymosis (178 cases, 66.42%) and skin burning (56, 20.90% ). Three month complication included painful induration or trabs (235 cases, 87. 69% ) as well as thrombophlebitis (28, 10. 45% ). Long-term complication (after 3 months) included skin numbness or pain at medial knee or ankle region (15,5. 60% ). Recanalization of CSV occurred in 4( 1. 49% ) treated limbs. Residual or reoccurred varicose veins were found in 13(4. 85% ) cases. Movement related swelling appeared in 6(2. 24% ) lower limbs. Conclusion EVLT is effective for patients with superficial varicosities of the lower extremities. Minor complications most often subsided within 3 months. Strict surgical protocol and high ligation of the GSV could prevent local recurrence of the varicose veins and the recanalization of GSV.
7.Diagnosis and Comprehensive Evaluation of Soil Fertility of Good Agricultural Practices Base for Spatholobus suberectus Dunn. in Pingyuan of Guangdong Province
Jincai LU ; Zhengzhou HAN ; Zhengjun WU ; Zhiming LAI ; Rui HE ; Shourong ZHANG ; Ruoting ZHAN
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(1):141-146
Objective To investigate the status of soil fertility of Good Agricultural Practices ( GAP) base for Spatholobus suberectus Dunn. (SSD) in Pingyuan county of Guangdong province, thus to provide reference for GAP research and the subsequent fertilization for SSD. Methods The deep layer and superficial layer of GAP soil were collected for the physiochemical detection and nutrient assay. Compared with the classification standard of the second national general soil investigation, single base soil fertility index was diagnosed and the comprehensive soil fertility was evaluated with modified Nemoro Index. Results The soil pH value and the contents of exchangeable calcium and magnesium were unbalanced, and the contents of macroelements of nitrogen and phosphonium, microelements, and organic matter were low. Therefore, the measures for improving the base soil fertility should be as follows: ( 1) soil amendments of bentonite, gypsum and slaked lime should be used to adjust the soil pH value; ( 2) each plant should be given 10 kg of slaked organic fertilizer as base fertilizer; ( 3) in the process of nurturing, some special micro-fertilizer solution should be used to treat the cut slips, and 5 kg of urea should be used for every 667 meter square of land; ( 4) besides compound fertilizer, every 667 meter square of land should be fertilized with 15 kg of ammonium dihydrogen phosphate for the supplement of nitrogen and phosphorus, and slaked lime and magnesium carbonate should be used for the supplement of soil moderate-quantity elements after transplantation. Conclusion The comprehensive fertility of Pingyuan GAP base for Spatholobus suberectus Dunn. is at low level, and should be improved in combination with GAP requirements.
8.Cervical radiculopathy treatment experts' consensus to establish radiculopathy type by using the modified Delphi meth-od
Lei ZANG ; Yong HAI ; Ning FAN ; Shibao LU ; Jincai YANG ; Qingjun SU ; Peng DU ; Yanjun GAO
Chinese Journal of Orthopaedics 2015;(9):890-897
Objective Cervical radiculopathy treatment experts' consensus to establish radiculopathy type by using the modified Delphi method. Methods Use document retrieval method to review information and articles about the treatment guidelines and articles of cervical radiculopathy including domestic and international areas, established a protocol about clini?cal consensus of the treatments for cervical radiculopathy. This protocol included 23 questions (the effective proportion of non?operating therapy, neck immobilization, physiotherapy, pharmacologic treatment, surgical indications, contraindications, anteri?or surgical decompression, anterior surgical implants). We performed a modified Delphi survey in which current professional opinions from experienced experts, representing from almost all of the Chinese provinces, were gathered. And then we modi?fied the protocol according to those professional opinions. Three rounds were performed and finally we established consensus. Consensus was achieved with ≥70% agreement. Results The panel included 30 experienced experts. The recycling question?naire's quantity of three rounds were 30(100%), 24(80%) and 16(53.3%) respectively. After three expert assessments, there were 18 questions which achieved with≥70%agreement and these questions accounted for 64.3%(18/28) of all the questions. Consen?sus of the treatments for cervical radiculopathy was reached on 7 aspects, including:the effective proportion of non?operating thera?py (1 question), neck immobilization (1 question), physiotherapy (1 question), pharmacologic treatment (5 questions), surgical indi?cations (3 questions), contraindications (4 questions), surgery (3 questions). Conclusion This modified Delphi study had reached a consensus concerning several treatment issues on cervical radiculopathy which had strong representativeness of experts and good convergence of opinions. In the absence of high?level evidence, at present, these experts' opinion findings will guide health care providers to define appropriate treatment in their regions. Areas with no consensus provide excellent insight for future research.
9.Clinical living-related segmental small bowel transplantation: a case report
Shufeng WANG ; Xiangming CHE ; Jincai CHEN ; Shaoying LU ; Xiongwei HUO ; Lin FAN ; Guowei LI
Chinese Journal of General Surgery 2000;0(12):-
Objective To investigate the effect of short bowel syndrome treated with living-related small bowel transplantation(SBT).Methods A male patient with residual intestine 20cm in length,which resulted from subtotal small bowel resection and right hemi-colectomy owing to intestinal volvulus,received a living-related SBT.The donor was the patient′s mother.Donor specific blood transfusion,50mL/per week,was carried out for 8 weeks.Cytomegalovirus infection status in both donor and recipient was negative.A 160cm segment of intestine was transplanted.The graft ileocolic artery and vein was anastomosed to the recipient′s infrarenal aorta and inferior vena cava end-to-side,respectively.A distal ileostomy was performed.(Immunosuppression),anti-infection and anticoagulation therapy,and nutritional support were given(postoperatively).Results The donor had an uneventful recovery.No technical complications were observed.The recipient was alive and well 31 weeks after operation.No graft rejection or infection was found.The(patient) was taken off TPN 8 weeks after operation,and got a low-fat meal.The result of D-xylose test was near normal.Conclusions Living-related small intestine transplantation is an effective treatment for short bowel syndrome.
10.Development and Application of Non-invasive Real-time Detection System for Viscoelasticity of Skin Tissue.
Siyu LU ; Jincai SUN ; Tong ZHANG ; Yilin SONG
Chinese Journal of Medical Instrumentation 2020;44(5):384-389
To satisfy the daily demand of skin condition maintenance, make non-invasive real-time detection, and get proper quantitative evaluation of skin viscoelasticity parameters at the same time, a portable non-invasive detection system to acquire real-time skin tissue viscoelasticity is developed. The system relies mainly on a single-degree-of-freedom forced vibration model, with spring-damp-mass, and on dynamic micro indentation method. The experiment is conducted on two kinds of springs, and on pigskin tissues as well, the system's suitability, accuracy and stability are confirmed. The skin viscoelasticity detection in vivo is also carried out on 20 subjects with different ages, the differences of skin viscoelasticity in various parts of the body are investigated, and the correlations between age and skin viscoelasticity are clarified.
Elasticity
;
Humans
;
Skin
;
Skin Physiological Phenomena
;
Time
;
Viscosity