Objective To investigate the clinical efficacy of double-plate fixation combined with bone graft on SchatzkerⅤ/Ⅵ tibial plateau fractures.Methods Totally 35 cases with tibial plateau fractures from April 2013 to October 2015 were selected into an observation group,and treated with dual plate fixation combined with bone graft.Another 35 cases with tibial plateau fractures from March 2011 to March 2013 were enrolled in a control group,and treated with conventional anatomical plate.The data of the two groups were analyzed.Results Pain scores in observation group were lower than those in the control group,and the knee joint function scores in observation group were higher than those in the control group (P＜0.05);the healing degree was 65.7％ in the control group,which is significantly less than that (91.4％) in the observation group (P＜0.05);the complication incidence rate was 8.6％ in the observation group which was obviously lower than that (34.3％) in the control group (P＜0.05).Conclusion Double-plate fixation combined with bone graft in the treatment of tibial plateau fracture shows a high efficacy,and is worthy of clinical application and promotion.

The 26S proteasome is a proteolytic complex responsible for the degradation of the vast majority of eukaryotic proteins. Regulated proteolysis by the proteasome is thought to influence cell cycle progression, transcriptional control, and other critical cellular processes. A novel Schistosoma japonicum gene (GenBank Accession No. AY813725) proteasome alpha2 subunit (SjPSMA2) was cloned. Sequence analysis revealed that the ORF of SjPSMA2 gene contains 708 nucleotides encoding 235 amino acids, and the molecular weight was estimated to be 25.84 kDa. Real-time PCR analysis showed that this gene expressed in 7 d, 13 d, 18 d, 23 d, 32 d and 42 d schistosoma. The mRNA level of SjPSMA2 was lower in 7 d and 23 d schistosomulum than that in other stages. The SjPSMA2 cDNA fragment was subcloned into an expression vector pET28a(+) and transformed into E. coli BL21 (DE3) cells. After induction with IPTCQ the 30 kDa fusion protein was produced as included bodies. Western-blotting revealed that the fusion protein could be recognized by the rabbit serum anti-Schistosoma japonicum adult worm antigen preparation, and the protein in native could be detected. After immunization of BALB/c mice with the fusion protein, the reduction rates of worm counts and liver egg counts were 12.33% and 35.23%. ELISA results revealed that the vaccinated group showed a significant increase in the level of IgG antibody. This study provided an important basis for investigating the regulation mechanism of the proteasome during the development of Schistosoma japonicum.
Animals ; Antibodies, Helminth ; blood ; Base Sequence ; Cloning, Molecular ; Escherichia coli ; genetics ; metabolism ; Genes, Helminth ; Helminth Proteins ; genetics ; metabolism ; Immunization ; Liver ; parasitology ; Male ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Parasite Egg Count ; Proteasome Endopeptidase Complex ; biosynthesis ; genetics ; immunology ; Rabbits ; Recombinant Proteins ; biosynthesis ; genetics ; immunology ; Schistosoma japonicum ; genetics ; metabolism ; Vaccines, Synthetic ; immunology