Several virus families have been shown to encode microRNAs (miRNAs), which have roles in the infection and replication of viruses in host cells. These virus-encoded miRNAs are identified in double-stranded DNA virus (dsDNA virus) and in several RNA virus families, but not in single-stranded DNA virus (ssDNA virus). We used a bioinformatics approach based on VMir, miRNAFold and MaturePred software to predict virus-encoded miRNA-like small RNAs from the genome of a ssDNA virus: Aedes aegypti densovirus (AaeDV). Northern blotting and stem-loop reverse transcription-polymerase chain reaction (RT-PCR) were used to detect predicted small RNAs. A miRNA-like small RNA termed "AaeDVMD" was identified by stem-loop RT-PCR from predicted candidates. This is the first report demonstrating that a ssDNA virus can encode miRNA-like small RNAs. These data will aid further exploration of the interaction between the AaeDV and its mosquito host.
Aedes ; virology ; Animals ; Base Sequence ; Computational Biology ; Densovirinae ; chemistry ; genetics ; metabolism ; MicroRNAs ; chemistry ; genetics ; metabolism ; Molecular Sequence Data ; RNA, Viral ; chemistry ; genetics ; metabolism

Objective To evaluate the curative effect of valsartan associated with low-dose amiodarone on the recurrence of atrial fibrillation (AF), the left atrial diameter (LAD), P wave dispersion (Pd) and the maximum P wave duration (Pmax) in patients with paroxysmal AF. Methods 76 patients with paroxysmal atrial fibrillation (PAF) were randomized to valsartan (test group) and placebo (placebo group), both associated with low-dose amiodarone, and were followed up for 18 months. The patients were asked to report any episode of symptomatic atrial fibrillation and to perform an ECG as early as possible. AF load, Pmax, Pd and LAD were measured before and at the 6th, 12th, and 18th month after the treatment. Results At least one ECG-documented episode of AF was reported in 16% of the patients in test group and in 41% in placebo group, the difference was significant (P

The aim of this experiment was to construct a human colorectal carcinoma cDNA phage expression library. Total RNA was extracted from the cancer tissue of human colorectal carcinoma, and the mRNA was purified. The single-strand and double-strand of cDNA were synthesized through reverse transcription-PCR and LD-PCR. cDNA fragments, after removal of those smaller than 500bp, were combined with ?TriplEx2 phage vector. The recombinant cDNA were packaged in vitro with MaxPlax TM Packaging Extract, then a small portion of packaged phage was used to infect E.coli XL1-blue for titration and determination of the percentage of recombinant clones. PCR method was used to identify the size of inserted cDNA. A human colorectal carcinoma cDNA phage library consisting of 2.07?10 6 pfu/ml recombinant bacteriophages was successfully constructed, the recombinant percentage was 94.5%, and the range of the fragment length of exogenous inserted cDNA was between 600bp～4kb, with an average of about 1.4kb. It met the universally accepted standards, and it could be useful in screening cDNA clones to find out the human colorectal carcinoma associated antigen genes.

Some anatomical aspects in application for the vein catheterization on the subclavian and other veins of the neck have been studied on 85 cadavers in Chinese adults.The subclavian vein measures 1.16cm in diameter and 3.76 cm in length. It lies 2 cm deep under the skin and muscles in the infraclavicular region. The angle between the vein and the clavicle is 38?. It usually lies behind the medial third of the clavicle and is separated from the subclavian artery, brachial plexus and the cupula of the pleura by the anterior scalenus muscle. This muscle is 1.19 cm in width and 0.37 cm in thickness, much thinner than the thickness mentioned in the previous literature. The distance from the origin of the subclavian vein to the opening of the superior vena cava into the right atrium is 13.85 cm on the right, and 16.36 cm on the left.The diameter of the internal jugular vein and its topographical relationship to the origin of the sternocleidomastoid muscle were also observed. The internal jugular vein is 1.26 cm in diameter, and lies under the clavicular head of the sternocleidomastoid muscle in 58.75%, between the two heads of this muscle in 41.25%.The external jugular vein measures 0.63cm in diameter, but its location and termination are more variable.The application of these data in vein catheterization was also discussed in this article.

Popliteal fossa dissections were performed on two hundred adult extremities and the patterns of the deep venous system were observed. Three general types were established for the popliteal vein and its branches. Single large trunk representing the popliteal vein at the level of the knee joint occurs only in 32.00％, two venous trunks at this level in 65.50％ and three venous trunks in 2.50％. Furthermore, considerable variations of the formation of popliteal veins were found. The patterns of popliteal vein were divided into three main types and eleven subtypes. Type Ⅱa occurs most frequently (41.50％). The size of each vein of the deep venous system was measured. The average calibre of the single popliteal vein is 6.89mm (ranging between 4.0-11.5 mm). The veins from the gastrocnemius muscle join the single or the two popliteal trunks respectively. The veins of the soleus muscle drain into the peroneal and posterior tibial veins. The short saphenous vein typically terminates in the popliteal vein. Variations in its termination were described.

The diaphyseal nutrient foramina were examined in 246 tibia and 212 fibula from complete sets of Chinese adult skeleton collection.The length and diameter of these bones were also measured.The number,position and orientation of the nutrient foramina were more variable in fibula than in tibia. Though the position of the nutrient foramina of the shaft of these bones were vari- able,however there was a restricted area in which most of the foramina were located. The nutrient foramina of the tibia usually situated on the posterior surface near the junction between the upper and middle thirds of its length,and that of the fibula was on the posterior or medial surface within the upper portion of the middle third segment. The nutrient artery to tibia and fibula were dissected and observed in 100 cases. The nutrient artery to tibia may have various sources of origin,it may arise from the posterior tibial,anterior tibial,and fibular arteries or directly from the popliteal artery. It has a larger calibre and runs over a longer course.The nutrient artery to fibula is relatively small and short,and all originate from the fibular artery. Certain practical applications of the arteries are briefly discussed.

Objective:To observe the resistance of hypericum japonicum Thunb.to helicobacter pylori.Methods:Liquid dilution method was used to culture mixture of hypericum japonicum Thunb.extractum and helicobacter pylori,hypericum japonicum Thunb.extraction and helicobacter pylori respectively.The minimum inhibitory concentration and minimal bactericidal concentration of hypericum japonicum Thunb.extractum and extraction were determined by comparing the growth condition of helicobacter pylori.Results:Both of hypericum japonicum Thunb.extractum and extraction had obvious resistant effect on helicobacter pylori.Conclusion:25 mg/ml hypericum japonicum Thunb.extractum and 6.25 mg crude drug/ml hypericum japonicum Thunb.extraction can suppress the growth of helicobacter pylori effectively.

The facial, retromandibular and external jugular veins of the neck were studied in a total of 230 Chinese cadavers.1. The external jugular vein can be divided into six types and fourteen subtypes. Type Ⅰ(40.22％) and type Ⅲ (29.13％) occurred more frequently in this series.2. The facial vein drains into the external jugular vein in 41.09％. It drains directly or indirectly into the internal jugular vein in 43.91％.3. The anterior branch of the retromandibular vein usually drains into the internal jugular vein and it runs through the space deep to the posterior, belly of the digastric and the stylohyoid muscles in 83.70％.4. The presence of the so called common facial vein occurred only in 29.13％.5. The external jugular vein often drains into the subclavian vein. It appears in 46.57％ in our observation.6. The external jugular vein crosses the inferior belly of the omohyoid muscle, when it runs downwards in the lower part of the external cervical triangle. It usually lies superficial to the omohyoid in 66.16％, deep to it in 26.35％.

This article is based on the observation of the temporomandibular joint(TMJ) and on the analysis of its morphological structure. The normal values of 15 items, used to represent the structure of the joint, have been established.Three indices, that is the glenoid fossa index, the articular tubercle index and the condyle index, are accepted to express the geometrical characters and to reflect the close relationship between the two functional parts of the joint. Besides, the frequency distributing curves are presented. It is concluded that the male's TMJ is eminently larger than the female's, while there is no distinct difference between left and right sides and between groups with or without occlusion. This result might meet the needs of the Clinical research as well as the design of the artificial TMJ.