The MDA contents, SOD and. GSH-Px activities of the heart, liver, brain, kidney and serum were observed systematically in isoprotercnol-induced myocardial infarction. The results suggested that the MDA contents, SOD and GSH-Px activities were increased in ischemic myocardium. Oxygen free radicals exerted detrimental effects on vitals organs, and also was accompanied by a compensatory increase of SOD and GSH-Px activities.

The effects of DMSO and nifedipine on experimental myocardial infarction induced by isoproterenol(ISP)were investigated.The results showed a significant reduction in myocardial FFA and MDA contents in DMSO and nifedipine-treated groups, as compared with the ischemic group, and a beneficial effect on activity of SOD,GSH-px and Ca~(2+)-Mg~(2+)-ATPase. The effects of OH. and Ca~(2+) in myocardialg infarction were further analysed in this study.

Changes in lipids, enzymes and free-radical system of myocardial membranes were investigated in isoproterenol-induced myocardial infarction so as to study the modulative effects of Svate on myocardial membrane injury. Animals were killed 24h after ISP(85mg/kg) injection. The results suggested that disturbances in lipids metabolism, enzymes and free-radical system in ischemic myocardium, indicating the existence of myocardial membrane injury. In the Svate(0.25U/kg)-treated group, a beneficial effect of Svate exerted on membrane injury, as indicated by increased activities of GSH-Px, SOD and Na~+K~+-ATPase, and decreased Ch/PL ratio, MDA contents and Ca~(2+)-Mg~(2+)-ATPase activities.

Objective To study the feasibility of TCI etomidate for total intravenous anesthesia (TIVA). Methods Forty patients scheduled for abdominal operation were divided into two groups with 20 cases each. Anesthesia in group E was induced and maintained with TCI etomidate 1-2 μg/ml and remifentanil 6 ng/ml,which in group P with TCI propofol 2-4 μg/ml and remifentanil 6 ng/ml to keep BIS 40-60. The perioperative changes of plasma glucose, cortisol, aldosterone and ACTH were Observed. Recovery from anethesia was recorded as well Results Both groups had the stabilized hemodynamics and glucose concentration. The plasma cortisol and ACTH concentrations in group E were decreased at the end of operation(P<0.05), but returned to preoperative level on the next day, which were not significantly changed perioperatively in group P. The time of eye opening was longer in group E than that in group P. Conclusion Etomidate has an inhibition on the function of the adrenal cortex temporally and can be used safely togather with remifentanil for TIVA in patients without hypoadrenocorticism.

Objective To investigate the clinical benefits and the impacts on distribution and antibiotic resistance of pathogenic bacterium associated with fluoroquinolone prophylaxis during neutropenia in patients with acute leukemia.MethodsA total of 62 infection episodes occurred in patients with acute leukemia were retrospectively analyzed from October 2013 to October 2015.The patients admitted in ward A (group A,n=31) received Cephalosporin drugs as contrast, and received quinolones in ward B (group B,n=31).ResultsTo observe the implementation of quinolones in treatment of patients, the bacteria decreased significantly, the Klebsiella pneumoniae was the most common, but compared with the control group, there are still differences (P<0.05, and the observation group of patients with oral ulcer infection rate was 9.68%, upper respiratory tract infection rate was 6.45%, the infection rate of lung 6.45%, the digestive tract infection rate was 6.45%, the infection rate was 3.23%, perianal skin infection rate was 3.23%, the infection rate was 3.23%, which is significantly lower than the control group (P<0.05, and the observation group of patients with severe neutropenia, duration of neutropenia lack degree is significantly better than the control group (P<0.05).ConclusionThe fluoroquinolone prophylaxis induces diminishing proportion of normal flora and increasing frequency of Escherichia coli in severely neutropenic patients with acute leukemia,may not influence the distribution of other bacteria.The susceptility of main pathogens may not be affected by antibiotic prophylaxis.The fluoroquinolone don't decrease the incidence of septicemia and infection in gastrointestinal tract.Our data suggest that more prudent use of antibiotic prophylaxis may be reasonable even in patients at high-risk for developing infection.

A Review] CD4+ T cells can be divided into Th1/Th2 subsets. Th1/Th2 imbalance participates many disease processes. A stable surface marker distinguishing Th1 and Th2 will greatly facilitate the investigation of Th1/Th2 interaction.Several surface molecules have been reported to be differentialy expressed between Th1 and Th2 cells.LAG-3,active ligands for P- and E-selectin ,IL-18R, IL-12R?2,CC chemokine receptor (CCR5) were shown to be dominantly expressed on Th1 cells,whereas expression of CD30,ST2L,CRTH2,CCR3,CCR4 was reported to be preferential to Th2 cells. In this review, several surface molecules were mainly discussed.

Objective: To explore behavioral problems of deaf students.Method:138 students (92 boys and 46 girls) were investigated and assessed by EMBU, the inventory for family backgrounds and Rutter Children Behavior Checklist, and their academic achievements were evaluated by their semester exams.Result:The detected rate of behavior problems was 61.6% by the teacher checklist. The correlation analysis showed that behavior problems in deaf students (especially in boys) correlated moderately with age, sex, academic grades, part of factors in EMBU and education (r=0.35～0.55,P

Objective To investigate the effects of nuclear transcription factor- kappa B (NF-!B) and activated protein- 1 (AP- l) on rheumatic myocardial fibrosis. Methods Thirty rheumatic myocardial specimens and 10 normal samples were examined by Masson′s staining, immunohistochemical staining and image analy- sis. Results Both NF- !B and AP- 1 were expressed in the nuclei of myocardium with rheumatic heart disease (RHD). The degree of positive staining in myocardiurn with RHD was significantly correlated with the cardial collagen volume (NF-!B: r=0.8945, AP- 1: r=0.9011, P

Objective To established a method for the quality standard of Shushenling Capsules. Methods Folium Perillae and Rhizoma Chuanxiong in Shushenling Capsules were identified by TLC. The content of emodin was determined by HPLC. Results The spots of samples on TLC can be well separated and the method had strong specificity. The averge recovery of emodin was 99.31% and RSD was 1.54%. Conclusion The method is simpie, sensitive and accurate. It can be used for quality control of Shushenling Capsules.

The aim of this experiment was to construct a human colorectal carcinoma cDNA phage expression library. Total RNA was extracted from the cancer tissue of human colorectal carcinoma, and the mRNA was purified. The single-strand and double-strand of cDNA were synthesized through reverse transcription-PCR and LD-PCR. cDNA fragments, after removal of those smaller than 500bp, were combined with ?TriplEx2 phage vector. The recombinant cDNA were packaged in vitro with MaxPlax TM Packaging Extract, then a small portion of packaged phage was used to infect E.coli XL1-blue for titration and determination of the percentage of recombinant clones. PCR method was used to identify the size of inserted cDNA. A human colorectal carcinoma cDNA phage library consisting of 2.07?10 6 pfu/ml recombinant bacteriophages was successfully constructed, the recombinant percentage was 94.5%, and the range of the fragment length of exogenous inserted cDNA was between 600bp～4kb, with an average of about 1.4kb. It met the universally accepted standards, and it could be useful in screening cDNA clones to find out the human colorectal carcinoma associated antigen genes.