Three different procedures of Guipi Oral Liquid have been studied. By comparison of TLC spots of n-buranol and n-hexane extracts from the sample and the UV absorption spectrum of the main component, ferulic acid, the rational production process was developed, which provides an experimental base for preparation improvement.

Objective To summarize the experiences of surgical treatment of infective endocarditis. Methods The treatment for 116 patients (53 male and 63 female) with bacterial endocarditis admitted consecutively from June 1996 to July 2002 was analyzed retrospetively. Their age ranged from 5 to 64 years (mean 35.8?10.6 years). Thirty-three patients underwent operations at active phase, as the others were operated after infection was controlled. Eradication of infective vegetations was done in 109 patients, and in 43 of them valvular replacement was also performed. Correction of congenital heart diseases was done in 68. In 3 patients, myxomas were removed. Results Two patients died before the operation, and 3 patients died after surgery. Serious postoperative complications included severe low cardiac output in 5 patients, multiple organ failure in 3, and re-infection in 3 patients. Conclusion Appropriate preoperation preparation, early surgical treatment, and management of complications should be emphasized in the treatment of infective endocarditis.

Objective To evaluate the role of membrane-bound complement regulatory protein expression in spinal cord dorsal horn in the development of neuropathic pain (NP) in rats.Methods Forty-eight male Sprague-Dawley rats,weighing 200-250 g,in which intrathecal catheters were successfully implanted without complications,were randomly divided into 4 groups (n =12 each):sham operation group (group S),group NP,normal saline group (group NS) and minocycline group (group M).NP was induced by chronic constrictive injury of the sciatic nerve in NP,NS and M groups.Minocycline 50 μg was injected intrathecally once a day for 7 consecutive days starting from 1 day before ligation of the sciatic nerve in M group,while the equal volume of normal saline was given in stead of minocycline in NS group.Mechanical pain threshold and thermal pain threshold were measured at 1 day before ligation of the sciatic nerve (baseline,T0) and 1,3,7 days after ligation of the sciatic nerve (T1-3).Then the rats were sacrificed at T3 and the lumbar segment (L4,5) of spinal cord was removed for determination of the expression of CD46,CD55,CD59 protein and mRNA in the dorsal horn of spinal cord by Western-blot and RT-PCR,respectively.Results Compared with S group,mechanical pain threshold and thermal pain threshold were significantly decreased at T1-3 and the expression of CD46,CD55 and CD59 protein and RNA was down-regulated at T3 in NP,NS and M groups (P ＜ 0.05).Compared with groups NS and NP,mechanical pain threshold and thermal pain threshold were significantly increased at T2.3 and the expression of CD46,CD55 and CD59 protein and RNA was up-regulated at T3 in M group (P ＜ 0.05).Conclusion The down-regulated expression of membrane-bound complement regulatory proteins in spinal cord dorsal horn and abnormal activation of the complement are involved in the development of NP in rats.

AIM: To study the dynamic changes of amino acid neurotransmitters during cerebral ischemia and reperfusion in awake rats. METHODS: Model of cerebral ischemia and reperfusion in awake rats was replicated. Glutamate (Glu), aspartate (Asp), ?-aminobutyric acid (GABA), glycine (Gly), taurine (Tau), alanine (Ala), serine (Ser), threonine (Thr) and glutamine (Gln) concentrations were measured with microdialysis, and excitotoxic index (EI) was calculated in dialysates of hippocampus, neo-cortex and striatum. RESULTS: The significant increases in extracellular not only excitatory amino acid neurotransmitters-Glu and Asp, and their neuromodulator-Gly, but also inhibitory amino acid neurotransmitter-GABA and its neuromodulator-Tau and Ala were observed. However, the EI, representing the balance of excitatory and inhibitory neurotransmitters, was significantly increased during ischemia. CONCLUSION: The results suggest that the elevated Glu and Asp levels during ischemia are insufficient to independently engender ischemic damage, and other neurotransmitters or other factors may play an important role in modulating the excitotoxic effects of Glu and Asp.

The arteries of the foot in one hundred Chinese adult specimens were dissected and examined. 1. The dorsalis pedis artery was absent in 4%, and it originated from an abnormal origin in 5%, its course and relationship with the tendon of the extensor hallucis longus and deep peroneal nerve were also described. 2. The anterior medial and anterior lateral malleolar arteries originated more commonly at the level of the ankle joint, and sometimes they may originate from the dorsalis pedis artery. 3. The origin and number of the medial tarsal artery were variable, but that of the lateral tarsal artery were more constant. 4. The arcuate artery was present in 35%, but only 17% was typical in form, from which the 2nd-4th dorsal metatarsal arteries were giving off. 5. The sources of the dorsal metatarsal arteries were the most variable. The first dorsal metatarsal arteries which arose from dorsal or plantar aspect were similar in percentages. The 2nd-4th dorsal metatarsal arteries often originated from the plantar aspect, but it was not uncommon that they came equally from dorsal and plantar aspects. 6. The artery of the tarsal sinus was usually present, it may be divided into two categories, namely the proximal and the distal artery, with their distinct origins and courses. 7. The arterial trunks in the sole of the foot were more constant. The posterior tibial artery usually branched into its two main branches near the lower border of the laciniate ligament. 8. The lateral plantar artery was greater than the medial in 82%. And 71% of the plantar arch was formed chiefly by the deep plantar branch of the dorsalis pedis artery. 9. The plantar metatarsal arteries originated usually from the plantar arch, sometimes the neighbouring arteries may have a common trunk. The course of the Ist-3rd plantar metatarsal arteries was constant, but that of the 4th may be variable. Finally, some questions about the arteries of the foot, present in previous literatures, were briefly discussed.

The facial, retromandibular and external jugular veins of the neck were studied in a total of 230 Chinese cadavers.1. The external jugular vein can be divided into six types and fourteen subtypes. Type Ⅰ(40.22％) and type Ⅲ (29.13％) occurred more frequently in this series.2. The facial vein drains into the external jugular vein in 41.09％. It drains directly or indirectly into the internal jugular vein in 43.91％.3. The anterior branch of the retromandibular vein usually drains into the internal jugular vein and it runs through the space deep to the posterior, belly of the digastric and the stylohyoid muscles in 83.70％.4. The presence of the so called common facial vein occurred only in 29.13％.5. The external jugular vein often drains into the subclavian vein. It appears in 46.57％ in our observation.6. The external jugular vein crosses the inferior belly of the omohyoid muscle, when it runs downwards in the lower part of the external cervical triangle. It usually lies superficial to the omohyoid in 66.16％, deep to it in 26.35％.

Objective The aim of this study was to investigate the prevalence of erectile dysfunction (ED) and to assess the risk factors of ED in male post‐stroke patients .Methods One hundred and twenty six male post‐stroke patients were invited to par‐ticipate in this study .Patients were divided into ED group and non‐ED group according to the erectile function evaluated by Interna‐tional Index of Erectile Function‐5 (IIEF‐5) .The neurological impairment was measured using the National Institute of Health Stroke Scale (NIHSS) and the modified Rankin Scale (mRS) .Depressive symptoms was measured using the Hamilton Depression Scales (HAMD) .The global cognitive function was measured using the Mini mental Status Examination (MMSE) .The clinical and psychosocial factors were compared between the ED and non‐ED groups .Univariate and multivariate Logistic regression analyses were employed to assess the risk factors with ED .Results There were 38 patients (30 .2% ) with ED in all the 126 male post‐stroke patients .Univariate Logistic regression analyses revealed that hypertension ,diabetes ,ACEI ,and depression were significantly related to ED in male post‐stroke patients (P<0 .05) .Multivariate Logistic regression analyses revealed that ACEI and depression were independently associated with ED in male post‐stroke patients (P<0 .05) .Conclusion ED is common in Chinese male post‐stroke patients .ACEI and depression are the major determinants of ED .

The veins on the dorsum of the foot have been dissected and observed on 200cases of the Chinese adult lower extremities.The great saphenous vein is the chief draining vessel of the hallux,the toes andthe skin of the dorsum of the foot.The position and the tributaries of this vessel areconstant.Its diameter ranges from 1.9 to 5.0mm,with an average of 3.2mm.The dorsal venous arch is usually single(93%),double arches are seen in 6%,and absent in 1%.According to the form of reflux,the dorsal venous arch may begrouped into five types.In the most common type,the arch is continuous withthe great saphenous vein and the anterior malleolar branch of the small saphenousvein(49.5%).The dorsal metatarsal veins usually drain directly into the dorsal venous arch.Some of the neighboring dorsal metatarsal veins may drain through a commontrunk.The perforating veins on the dorsum of the foot may be divided into threedifferent groups:the anterior malleolar,marginal and intermetatarsal group.Thelatter usually drains into the dorsal venous arch at the base of the first intermeta-tarsal space.The valves of the superficial veins on the dorsum of the foot were also observed.There is no valve in the lateral part of the dorsal venous arch,but there may beone or two valves in its most medial part(66.7%).It appears that the venousblood from the first metatarsal vein usually drains into the great saphenous vein.

OBJECTIVE: To screen the anti-tumor fraction of ethanol extracts from Thymus quinquecostatus Celak and investigate its anti-tumor effect on human leukemia cell line. METHODS: Ethyl acetate, n-butanol and acetone fractions were separated from the ethanol extracts of wild Thymus quinquecostatus Celak. Growth inhibiting effects of these extracts on human leukemia cell lines K562 and HL-60 were determined by live cell counting and cell growth curve analysis. The possible anti-tumor mechanism was studied by morphological analysis with norcantharidin as a positive control. RESULTS: Ethyl acetate fraction could significantly inhibit the proliferations of K562 and HL-60 cells, and the inhibiting effect depended on the concentration of ethyl acetate fraction. Ethyl acetate fraction could induce apoptosis of K562 and HL-60 cells. The n-butanol and acetone fractions had no significant inhibiting effect on K562 and HL-60 cells. CONCLUSION: Ethyl acetate fraction is the major anti-tumor fraction in ethanol extracts from Thymus quinquecostatus Celak.

Objective To study the imaging characteristics of the ligaments in craniocervical junction (CCJ), and to optimize the examination methods and scanning sequences of the ligaments in this region. Methods Two groups of 51 healthy volunteers in each were selected to undergo CT and MRI examination respectively. The CT and MRI features of the ligaments in CCJ were assessed. Two senior imaging doctors compared the results of showing the ligaments in CCJ by several MRI sequences, including T1 WI ,T2 WI, proton density weighted imaging (PDWI), T2 * WI and short time inversion recovery (STIR) ,and proton density fat saturate (PDFSAT). Standard normal rank transformation was done according to the primary data,and then analysis of variance of repeated measurement was applied. Results CT and MRI could both demonstrate the ligaments (except the anterior atlantooccipital membrane only seen on MRI) and their adjoins in CCJ, while MRI had more advantages than CT. The display ratio of the anterior atlantooccipital membrane was 100% (51/51) by MRI. The display ratio of the apical ligament was 29.4%(15/51) by CT and 43.1% (22/51) by MRI. The posterior atlantooccipital membrane-dura complex,tectorial ligament-dura complex, transverse ligament, and alar ligament could be demonstrated clearly by CT and MRI, the display ratio was 100% respectively (51/51). The results of PDWi scored by two doctors were both 5.0, there were no significant differences between them (F = 0.000, P > 0.05), which were significantly higher than T, WI (M = 3.0), T2Wi (M = 3.0), T2 * WI (M = 1.0), STIR (M = 1.0), and PDFSAT(M = 3.0)(P < 0.01). Conclusion MRI was superior to CT in demonstrating the ligaments in CCJ. PDWI was the optimal MRI sequence. Imaging research of normal ligamentous structures in CCJ could provide valuable diagnostic and therapeutic information for evaluating the ligamentous diseases.