Objective: To establish the in vitro and in vivo HPLC fingerprint of Shuanghuanglian Oral Liquid (SOL). Methods: The chromatographic fingerprint was obtained with Welchrom C18 column (150 mm × 4.6 mm, 5 μm); The mobile phase was methanol-0.2% phosphoric acid aqueous solution, with gradient elution and segmentation variable wavelength determination; The column temperature was maintained at 30°C; The injection volume was 10 μL. Results: Among 10 batches of samples prepared by single and different preparation methods, the overall similarity evaluation was taken using the Chromatographic Fingerprint Similarity Software for Chinese Materia Medica (Version 2004A), and the average similarities were all above 0.93. The better in vivo and in vitro correlation of the index constituents in SOL prepared by the method of Pharmacopoeia of People's Republic of China was obtained. Conclusion: The method had good precision, stability, and repeatability. The standard chromatographic fingerprint established has a better representativity, and the method provides the new ideas for establishing the new evaluation system of Chinese materia medica oral preparation.

Objective: To establish a lung cancer phage-peptide library and to screen for biomarkers for early detection of lung cancer. Methods: A phage display library was constructed using 30 lung-cancer tissue samples from Changhai Hospital. Protein-A/G agarose was used to enrich IgG from control sera as well as lung cancer sera. Five biopannings were carried out for enrichment of lung cancer-specific phage clones. Five hundred phage clones were randomly selected,those with D>2.0(lung cancer plasma pool v. s. control plasma pool) were selected for DNA sequencing and protein prediction. Results: (1) The recombination rate of the phage library was 60%,with the average phage titer being 3.0 × 106 pfu and a volume being 9.0 × 106 pfu. (2) Of 19 phage colonies selected by ELISA and sequenced,9 were cancer-related genes,8 with unknown function, and 2 were not related to cancer. Conclusion: The screened genes in the phage colonies might serve as biomarkers for the early diagnosis of lung cancer.

Objective: To study the correlation between responses to Hepatitis B and HLA DRB1 * 07 DRB1 * 04 DRB1 * 1001, DQB1 * 0401 genes in Han population in Ningbo. Methods: A total of 240 Han people living in Ningbo received the routine vaccination of recombinant hepatitis B vaccine. The serum levels of anti-HBs antibody were examined and the subjects were divided into 2 groups according to the results: negative responses group (n = 120, anti-HBs D< 0.1) and positive response group (n = 120, anti-HBs D≥0.1). The distribution and frequency of DRB1 * 07, DRB1 * 04, DRB1 * 1001, DQB1 * 0401 genes alleles were determined with PCR using sequence-specific primers (PCR-SSP). Results: The sex and age were compatible between the 2 groups. The frequency of DRB1 * 07 in negative response group was significantly higher than that in positive response group (20.3%, vs 10.3%, P<0.05); the failure of vaccination was correlated with DRB1 * 07 (OR=0.45, P<0.05). The frequencies of HLA- DRB1 * 04 DRB1 * 1001, DQB1 * 0401 showed no obvious difference between the 2 groups. Conclusion: Failure or deficiency response to HBV vaccine in the Han people in Ningbo is associated with the HLA-DR antigen; HLA-DRB1 * 07 might be a susceptible gene for failure of HBV vaccination.

Objective: To investigate the first appearance and geographic distribution of hantaviruses in China. Methods: Some sequences of M segment of hantaviruses isolated in China were retrieved from GenBank. Some recently sequenced viruses which have not been registered in GenBank were obtained from authors who reported them. Program MEGA 3.1 was employed for the phylogenetic analysis of the above virus sequences. Nucleotide divergence rates were estimated by comparing the closely related sister sequences isolated in the same geographical region at no less than a 5-year interval. Based on these calculated divergence rates, together with their isolating time and region, we explored the first appearance and geographic distribution of hantaviruses in China. Results and conclusion: The distribution of hantaviruses was in cluster, with Guizhou Province having the most subgenotypes, which supports the previous hypothesis that hantaviruses first appeared in Guizhou Province of China. A novel subgenotype virus was found in Anhui Province, indicating that it might be easier for the virus to have mutation in Anhui Province. We also found that Gou3 strain might be the earliest one to separate from a common ancestral strain; it is speculated that hantaviruses migrated in China about 315 years ago.

Objective: To investigate the effects of enamel matrix pr ot eins(EMPs) and purified EMPs(EMD) on the proliferation and protein synthesis act ivity of rat ectomesenchymal stem cells in vitro. Methods: C ell culture technique and 3H-leucine label assay were used to measure the pr oliferation and protein synthesis activity of rat ectomesenchymal stem cells exp osed to the EMPs- or EMD-conditioned culture media with different concentratio n. Results: Both EMPs and EMD at 50～200 mg/ml increased the pro l iferation and protein synthesis of the cells in 10-day-culture. EMPs and EMD a t 150 mg/ml showed the strongest effects(P

Objective To evaluate if the enteral nutrition have effect on the immune function and in- flammatory reaction after operating about gastric carcinoma.Methods 58 postoperative patients suffering from stomach cancer and colon cancer were randomly divided into the EN group and the TPN group.On the first postoperative day,nutrition fibre were given via nasal intestinal tube,increasing the capacity and drop- ping speed day by day until patients can eat liquid diet.While patients in the TPN group didn't eat anything until enterokinesia completely recovered.Observing on preoperative day 1 and on postoperative day 3 and day 8 respectively to check IgA,IgG,IgM,C3,C4,CRP,LYM,LYM%,TP,ALB,PA.Results The results showed that on the postoperative day 8,the target ascension extent was higher than that in the PN group.The statistical significance was very obvious(P

Objective To compare the results of three methods for measuring C reaction protein.Methods 100 patients were collected from our hospital,and three different methods for measuring C reaction protein were used to analyze the level of C reaction protein.Results Correlation analysis showed that the correlation between immunoturbidimetric assay and immunochromatography was higher.The differences of three methods assayed the C reaction protein were significant (P <0.05 )among normal people by the variance analysis,but had not statistical significance among abnormal people (P >0.05).The differences among all of test peo-ple were significant(P <0.05).Conclusion Detection of C reaction protein was important for diagnose inflammatory diseases.

Genomics ; methods ; Humans ; Microbiology

Alzheimer’ s disease ( AD) is one of the most common dementia of neurodegenerative disorders, which results from the deposition of amyloid-beta ( Aβ) and there are no curative treatments for this disease at present.It had been proved that prion protein is the receptor for Aβand it plays a key role in the progress of AD with dual-side effects. Prion protein can not only transfer neurotoxicity to neurons but also protect them from neurotoxicity of Aβ.The polymor-phisms of prion protein encoding gene ( PRNP) affect the AD incubation period and clinical symptoms in humans and other animals.The discovery of PRNP mutational mouse fills the gaps of existing AD mouse models in this research area, which is potential for the studies of pathogenesis, new drugs design and testing aspects.The role and effects of prion protein in AD pathogenesis were summarized in this paper, furthermore, the discovery and utility of PRNP gene mutational mouse in research on AD and/or amyloid diseases were reviewed, and in order to provide some guidance for AD animal model study.

Animals ; Disease Models, Animal ; Heart Rate ; Hypertension ; physiopathology ; Rats ; Rats, Wistar