OBJECTIVE To analyze the incidence and relevant factors of hospital infection of newborn baby in a mother with own baby room,to put forward the corresponding nursing countermeasures,as a result,and to probe into the nursing ways for decreasing the incidence of hospital infection of newborn baby in the same conditions.METHODS A retrospective analysis was made on the monitoring material concerning the hospital infection of newborn babies,who were born in our hospital from 2001 to 2005,in a mother with own baby room.RESULTS The average sick rate of hospital infection of newborn baby in the same conditions was 1.12%.Among them,3.10% was in 2001,1.62% in 2002,1.09% in 2003,0.32% in 2004 and 0.05% was in 2005.And the most was respiratory tract infection,in which the key pathogen causing the infection was Staphylococcus epidermidis.CONCLUSIONS In order to decline the sick rate of hospital infection of newborn baby living in a mother with own baby room,room conditions should be carried out by air disinfection,using disinfected nursing articles and simultaneously doing well the respiratory tract nursing and basic nursing.

OBJECTIVE To transfer the dispersing management non-disposable medical instrument packages into the centralized management by the supply department,to improve its quality and save the medical cost.METHODS The centralized management adopted the step by step implementation strategy,standardized recycling,cleaning,and packaging procedure for key instruments.The cleanliness and integrity of the packaging sheet,the in-packet indicator cards and the out-packet label,and instrument and their human and material resources consumption were compared each other.RESULTS Under the centralized management,the cleanliness and integrity of packaging sheet,the wholeness indicator cards in packet and outside packet of instrument label were obviously better than dispersing management(P

Objective:To observe autophagy in human peripheral blood T lymphocyte.Methods:Peripheral blood T lymphocytes of healthy adults were separated with Percoll(1.073 g/ml) and harvested by using nylon column. The cultured cells were divided into control and dexamethasone(DXM) group, and cell morphology was observed through light microscope, electron microscope and fluorescent microscope at 0 and 72 h. And incidence rate of autophagy was analyzed by flow cytometry.Results:①The natural cultured T lymphocytes showed typical morphology of autophagy. ②There was significant statistic difference between 0 and 72 h incidence rate of autophagic T lymphocyte in both control and DXM group. ③There was significant statistic difference in 72 h incidence rate of autophagic T lymphocyte between control and DXM group.Conclusion:Autophagy can be seen in human peripheral blood T lymphocytes, and DXM could induce autophagy.

Acute Disease ; China ; Humans ; Insecticides ; poisoning ; Organophosphate Poisoning ; Poisoning ; prevention & control

Accidents, Occupational ; prevention & control ; Humans ; Metals ; poisoning

Humans ; Pesticides ; poisoning ; Poisoning ; prevention & control

Humans ; Occupational Diseases ; diagnosis ; Reference Standards

Objective:To study the morphology and function of the dendritic cells(DC) from human peripheral blood monocytes and the change in cell differentiation and maturation after DC treated with dexamethasone(Dex-DC).Methods:DCs were sorted from human monocytes by culturing in presence of cytokines(GM-CSF, IL-4) and LPS for 8 days with or without dexamethasone. The change in morphology, function and phenotypic characterization was compared.Results:The cells treated with or without Dex, developed a characteristic dendritic morphology, however, Dex-DC expressed higher level of CD14 and lower level of CD83 than the untreated cells. In addition, a low APC function was demonstrated by Dex-DC.Conclusion:DCs can be induced from peripheral blood monocytes in medium with cytokines; the cultured PS in presence of Dex are shown at a more immature stage, indicating that Dex modulates DC differentiation, maturation, and function.

Objective:To optimize the preparation technology of ketoconazole and miconazole nitrateβ-cyclodextrin inclusion com-pound. Methods: The weight ratio of β-cyclodextrin to ketoconazole, inclusion temperature and inclusion time as the testing factors, the optimal inclusion technology was screened by orthogonal experiments. Results:The optimum inclusion conditions were as follows:the weight ratio of β-cyclodextrin to ketoconazole was 8 ∶1, the inclusion temperature was 50℃, and the ultrasonic time was 50 min. Conclusion:The optimized β-cyclodextrin inclusion process is simple and convenient to carry out.

Objective:To analyze the clinical therapeutic effect and safety of large dosage of valsartan on chronic heart failure (CHF).Methods:A total of 106 CHF patients hospitalized in our department were chosen and ran-domly divided into routine dose group (n=53,received routine dosage of valsartan,80mg,once/d)and large dose group (n=53,received large dosage of valsartan,80mg,twice/d)accerding to number table.Both groups received anti-heart failure treatment for six months according to the guidelines.After treatment,plasma N terminal pro brain natriuretic peptide (NT-proBNP)level was measured,left ventricular end-systolic diameter (LVESd),left ventricu-lar end-diastolic diameter (LVEDd),interventricular septal thickness (IVST)and left ventricular posterior wall thickness (LVPWT)were measured by color Doppler echocardiography,and all patients received 6min walking test. Therapeutic effect and incidence rates of adverse reactions were compared and analyzed between two groups after treatment.Results: Compared with routine dose group,there were significant reductions in NT-proBNP level [(3042.6±116.3)pmol/L vs.(2565.8±98.2)pmol/L],LVESd [(34.5±2.2)mm vs.(29.4± 2.0)mm], LVEDd [(55.1±2.9)mm vs.(50.2±2.5)mm],IVST [(12.9±1.8)mm vs.(10.7±1.2)mm]and LVPWT [(11.8±1.1)mm vs.(10.9±0.9)mm];significant rise in 6min walking distance [(271.2±24.9)m vs.(367.7 ±22.3)m]and total effective rate (43.40% vs.62.26%)in large dose group,P <0.05 all.There was no signifi-cant difference in incidence rates of major adverse reactions between two groups (P >0.05).Conclusion:Large dos-age of valsartan is more effective and is safe in treatment of chronic heart failure.