OBJECTIVE To analyze the incidence and relevant factors of hospital infection of newborn baby in a mother with own baby room,to put forward the corresponding nursing countermeasures,as a result,and to probe into the nursing ways for decreasing the incidence of hospital infection of newborn baby in the same conditions.METHODS A retrospective analysis was made on the monitoring material concerning the hospital infection of newborn babies,who were born in our hospital from 2001 to 2005,in a mother with own baby room.RESULTS The average sick rate of hospital infection of newborn baby in the same conditions was 1.12%.Among them,3.10% was in 2001,1.62% in 2002,1.09% in 2003,0.32% in 2004 and 0.05% was in 2005.And the most was respiratory tract infection,in which the key pathogen causing the infection was Staphylococcus epidermidis.CONCLUSIONS In order to decline the sick rate of hospital infection of newborn baby living in a mother with own baby room,room conditions should be carried out by air disinfection,using disinfected nursing articles and simultaneously doing well the respiratory tract nursing and basic nursing.

OBJECTIVE To transfer the dispersing management non-disposable medical instrument packages into the centralized management by the supply department,to improve its quality and save the medical cost.METHODS The centralized management adopted the step by step implementation strategy,standardized recycling,cleaning,and packaging procedure for key instruments.The cleanliness and integrity of the packaging sheet,the in-packet indicator cards and the out-packet label,and instrument and their human and material resources consumption were compared each other.RESULTS Under the centralized management,the cleanliness and integrity of packaging sheet,the wholeness indicator cards in packet and outside packet of instrument label were obviously better than dispersing management(P

Objective:To observe autophagy in human peripheral blood T lymphocyte.Methods:Peripheral blood T lymphocytes of healthy adults were separated with Percoll(1.073 g/ml) and harvested by using nylon column. The cultured cells were divided into control and dexamethasone(DXM) group, and cell morphology was observed through light microscope, electron microscope and fluorescent microscope at 0 and 72 h. And incidence rate of autophagy was analyzed by flow cytometry.Results:①The natural cultured T lymphocytes showed typical morphology of autophagy. ②There was significant statistic difference between 0 and 72 h incidence rate of autophagic T lymphocyte in both control and DXM group. ③There was significant statistic difference in 72 h incidence rate of autophagic T lymphocyte between control and DXM group.Conclusion:Autophagy can be seen in human peripheral blood T lymphocytes, and DXM could induce autophagy.

Thirty cases of obesity were treated by acupuncture plus auricular point-embedding method based upon pattern identification, and another 30 cases were treated by single auricular point-embedding method for control study. After the treatments for 30 days, the effective rate was respectively 90.0% and 66.7%, with the statistic significance (P＜0. 05) between the therapeutic effects of the two groups.

Moral hazard in the catastrophic disease health insurance has challenged the sustainability of med-ical budget funds. This paper studies the health care reimbursement rule to counteract moral hazard. Theoretical a-nalysis shows that in the target to maximize social welfare, the optimal rule is to provide consumers who choose low-cost treatment with subsidies and charge a co-payment to those who choose high-cost one. Adopting simulation ap-proach shows that this difference with respect to single reimbursement rules, healthcare expenses and medical insur-ance premiums will be significantly reduced compared to a unified co-payment ratio. This paper also selects the sensitivity test simulation parameters and the results show that different values will not change the herein disclosed mechanism results.

Based on the high level of human granulocyte—macrophage colony—stimulating factor(HGM—CSF)re-ceptor of HL60 cell line upon induction by DMSO,we developed a hioassay method for HGM—CSF with ahigher specificity and sensitivity.The sensitivity of this assay reached to 0.3ng/ml by selecting the optimalHL60 cell—induced time,cell density and GM—CSF—stimulated time.With this assay,we are able to quantifythe level of rHuGM—CSF as well as conditioned media from human bladder carcinoma cell line U5637 screatinga high level of GM—CSF.

Objective:To study the morphology and function of the dendritic cells(DC) from human peripheral blood monocytes and the change in cell differentiation and maturation after DC treated with dexamethasone(Dex-DC).Methods:DCs were sorted from human monocytes by culturing in presence of cytokines(GM-CSF, IL-4) and LPS for 8 days with or without dexamethasone. The change in morphology, function and phenotypic characterization was compared.Results:The cells treated with or without Dex, developed a characteristic dendritic morphology, however, Dex-DC expressed higher level of CD14 and lower level of CD83 than the untreated cells. In addition, a low APC function was demonstrated by Dex-DC.Conclusion:DCs can be induced from peripheral blood monocytes in medium with cytokines; the cultured PS in presence of Dex are shown at a more immature stage, indicating that Dex modulates DC differentiation, maturation, and function.

Objective To study the clinical value of preoperative superselective embolization of nasopharyngeal angiofibroma.Methods 14 patients with nasopharyngeal angiofibroma diagnosed by CT and MRI,and proved surgically and pathologically were included in this study.Angiography of bilateral external carotid artery and internal carotid artery as well as vertebral artery was performed in all cases. The external carotid arterial blood supply of tumors were superselectively embolized with 100～300 ?m particles of gelfoam or polyvinyl alcohol(PVA). Surgical resection was performed post-embolization 1 to 3 days. Results The main feeding vessels of tumors were the internal maxillary artery in 9 patients,the internal maxillary artery and ascending pharyngeal artery in 4 patients,the internal maxillary artery,ascending pharyngeal artery and the branches of internal carotid artery in one patient. Preoperative endovascular embolization of the branches of external carotid artery with microcatheter was achieved in all patients. After embolization,tumor stains disappeared completely in 8 patients and partially in 6 patients. The rate of total resection was 92.8%(13/14).The mean amount of haemorrhage during operation was 420 ml and the mean operation duration was 150 min.No major complications were relevant to embolization occurred.Conclusion Preoperative intravascular embolization of nasopharyngeal angiofibroma with microcatheter is a safe and effective method.

Objective:To observe oncosis in human peripheral blood T lymphocytes.Methods:Peripheral blood T lymphocytes of healthy adult was separated with Percoll(1.073 g/ml)and harvested by using nylon column.The cultured cells were divided into control and dexamethasone(DXM)group,and cell morphology was observed through light microscope,electron microscope and fluorescent microscope.And incidence rate of oncosis was analyzed by flow cytometry.Results:①Oncosis could be observed in cultured T lymphocyte after 96h.②In different concentrations of DXM group(1?10-6,1?10-5,1?10-4,1?10-3mol/L),The incidence of oncosis T lymphocytes was(3.49?0.42)%,(5.17?0.48)%,(8.44?0.72)%,(17.93?1.50)%.③During different cultured period(48,72,96,120h),The oncositic rate of T lymphocytes in DXM group was(0.53?0.10)%,(6.36?0.80)%,(20.60?1.59)%,(25.56?1.76)%.Conclusion:Oncosis can be seen in human peripheral blood T lymphocytes,and DXM could induce oncosis.

Objective:To optimize the preparation technology of ketoconazole and miconazole nitrateβ-cyclodextrin inclusion com-pound. Methods: The weight ratio of β-cyclodextrin to ketoconazole, inclusion temperature and inclusion time as the testing factors, the optimal inclusion technology was screened by orthogonal experiments. Results:The optimum inclusion conditions were as follows:the weight ratio of β-cyclodextrin to ketoconazole was 8 ∶1, the inclusion temperature was 50℃, and the ultrasonic time was 50 min. Conclusion:The optimized β-cyclodextrin inclusion process is simple and convenient to carry out.