OBJECTIVE To analyze the incidence and relevant factors of hospital infection of newborn baby in a mother with own baby room,to put forward the corresponding nursing countermeasures,as a result,and to probe into the nursing ways for decreasing the incidence of hospital infection of newborn baby in the same conditions.METHODS A retrospective analysis was made on the monitoring material concerning the hospital infection of newborn babies,who were born in our hospital from 2001 to 2005,in a mother with own baby room.RESULTS The average sick rate of hospital infection of newborn baby in the same conditions was 1.12%.Among them,3.10% was in 2001,1.62% in 2002,1.09% in 2003,0.32% in 2004 and 0.05% was in 2005.And the most was respiratory tract infection,in which the key pathogen causing the infection was Staphylococcus epidermidis.CONCLUSIONS In order to decline the sick rate of hospital infection of newborn baby living in a mother with own baby room,room conditions should be carried out by air disinfection,using disinfected nursing articles and simultaneously doing well the respiratory tract nursing and basic nursing.

OBJECTIVE To transfer the dispersing management non-disposable medical instrument packages into the centralized management by the supply department,to improve its quality and save the medical cost.METHODS The centralized management adopted the step by step implementation strategy,standardized recycling,cleaning,and packaging procedure for key instruments.The cleanliness and integrity of the packaging sheet,the in-packet indicator cards and the out-packet label,and instrument and their human and material resources consumption were compared each other.RESULTS Under the centralized management,the cleanliness and integrity of packaging sheet,the wholeness indicator cards in packet and outside packet of instrument label were obviously better than dispersing management(P

Objective:To observe autophagy in human peripheral blood T lymphocyte.Methods:Peripheral blood T lymphocytes of healthy adults were separated with Percoll(1.073 g/ml) and harvested by using nylon column. The cultured cells were divided into control and dexamethasone(DXM) group, and cell morphology was observed through light microscope, electron microscope and fluorescent microscope at 0 and 72 h. And incidence rate of autophagy was analyzed by flow cytometry.Results:①The natural cultured T lymphocytes showed typical morphology of autophagy. ②There was significant statistic difference between 0 and 72 h incidence rate of autophagic T lymphocyte in both control and DXM group. ③There was significant statistic difference in 72 h incidence rate of autophagic T lymphocyte between control and DXM group.Conclusion:Autophagy can be seen in human peripheral blood T lymphocytes, and DXM could induce autophagy.

Objective:Through the ale tissue in patients with rheumatic heart disease ( RHD) autoantigen cells change and pe-ripheral blood indicators of detection and IL-10,TGF-beta 1 discusses the relationship between serum concentration.Methods:cases of RHD patients and 63 healthy volunteers in the morning on an empty stomach acquisition anticoagulation peripheral venous blood,used flow cytometry assay of CD4+CD25+,signature molecules of regulatory T cells ( Foxp3 Treg) for testing,the proportion of the ELISA de-tection of IL-10,concentration of TGF-beta 1;Corresponding antigen gene expression library construction of rheumatic heart disease screening.Results:The autoantigen RHDAG1 has obvious differences with the control,RHD patients serum Treg ratio is lower than the control group in the sample data,IL-l0,TGF-beta 1 has the difference between the concentration of serum,compared the statistically difference between groups.Conclusion:Autoantigen RHDAG1 have certain effect on the development of the expression of RHD, the concentration and Treg cells on peripheral blood related indexes change detection has a certain clinical significance to the diagnosis of RHD.

Objective To determine the expression of Aquaporin 8 (AQP8) in the fetal membrane and placenta of idiopathic polyhydramnios. Methods The amnion, chorion and placenta were collected from 12 term pregnancies with idiopathic polyhydramnios(polyhydramnios group) and 12 term pregnancies who were normal (control group). The expression of AQP8 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of AQP8 protein was detected by immunohistochemistry. Results The expression of AQP8 mRNA in amnion, chorion and placenta of polyhydramnios group was (0.78±0.13), (0.58±0.10), and (0.86±0.15) respectively, and that of control group was (0.39±0.07 ), (0.45±0.09),and (0.34±0.09) respectively. The expression of AQP8 protein in amnion, chorion and placenta of polyhydramnios group was (0.195±0.024), (0. 170±0.028), and (0. 193±0.024) respectively, and that of control group was (0. 151±0.018), (0.156±0.024), and (0. 152±0.023) respectively. In all 3 types of tissues the expression of AQP8 mRNA of polyhydramnios group was higher than that of control group (P<0.05). In anmion and placenta the expression of AQP8 protein of polyhydramnios group was also increased compared to that of control group (P<0.05), but in chorion the difference in AQP8 protein expression between the two groups was not significant(P>0.05). Conclusion The expression of AQP8 mRNA and protein is significantly increased in the anmion and placenta of polyhydramnios, suggesting that AQP8 may play an important role in the regulation of amniotic fluid volume.

Objective To investigate the efficacy of laparoscopic cholecystectomy (LC) for the treatment of patients with cholelithiasis complicating liver cirrhosis. Methods The clinical data of 50 patients with cholelithiasis complicating liver cirrhosis were analyzed retrospectively, who received LC (LC group,23 cases) or open cholecysteetomy (OC group,27 cases). Summarized and compared with operation time, blood loss,length of stay and complications of two groups. Results Operation time,blood loss and length of stay in LC group [(43.2 ± 15.6) min, ( 78.3 ± 22.5 ) ml, (5.7 ± 2.5 ) d] were significantly less than those in control group [(77.4 ±21.2) min, (195.7 ±32.4) ml, (9.3 ±3.2) d.], the differences were statistically significant (P ＜ 0.05). The mainly post-operative complications were dehydration and urinary tract infection, but the incidence rate had no statistically significant difference between two groups (P ＞ 0.05 ).There was no other severe complications and death case occurred postoperation in two groups. Conclusion For patients with cholelithiasis complicating liver cirrhosis in Child-Pugh A and B class, LC is a safe and feasible minimally invasive surgery, it is worthy to be popularized.

Objective:To observe oncosis in human peripheral blood T lymphocytes.Methods:Peripheral blood T lymphocytes of healthy adult was separated with Percoll(1.073 g/ml)and harvested by using nylon column.The cultured cells were divided into control and dexamethasone(DXM)group,and cell morphology was observed through light microscope,electron microscope and fluorescent microscope.And incidence rate of oncosis was analyzed by flow cytometry.Results:①Oncosis could be observed in cultured T lymphocyte after 96h.②In different concentrations of DXM group(1?10-6,1?10-5,1?10-4,1?10-3mol/L),The incidence of oncosis T lymphocytes was(3.49?0.42)%,(5.17?0.48)%,(8.44?0.72)%,(17.93?1.50)%.③During different cultured period(48,72,96,120h),The oncositic rate of T lymphocytes in DXM group was(0.53?0.10)%,(6.36?0.80)%,(20.60?1.59)%,(25.56?1.76)%.Conclusion:Oncosis can be seen in human peripheral blood T lymphocytes,and DXM could induce oncosis.

Objective: To investigate the inspection mode of ethical issues for multicenter clinical trails in China, and to make analysis and give suggestions while taking into consideration of the international conventions. Methods: Representatives from 21 general hospitals and specialized hospitals from Liaoning province, Jiangsu province, Sichuan province, Guangdong province and Shanghai participated in a training course on promotion of ethical review capability; they were surveyed by anonymous questionnaire. Another 20 clinical trial agencies and ethical committees were interviewed by telephone. The questionnaires were collected and valid questionnaires were subjected to statistical analysis. Results: We found that 65.1% of the interviewees never used a central institutional review board (IRB); the main reasons included that they could not assess the quality of central IRB, they wanted to protect the subjects, they had a poor communication with the IRB, and they could not obtain a satisfactorily-localized informed consent forms. There were 34.9% of the interviewees used a central IRB, because they wanted to shorten the inspection time, avoid influence to the research progress, and to have satisfactory quality of IRB. Conclusion: Central IRB is not widely accepted. The related system needs to be further completed so as to improve the outcome of the inspection. The communication and cooperation between the central and local IRBs should be strengthened.

In order to explore the effect of Yaoliuan capsule in the prevention and treatment of vaginal bleeding after drug-induced abortion and menses recovery after drug-induced abortion, 323 cases of gestation period < or = 49 days and without contraindication, were divided randomly into study group (168 cases, taking Yaoliuan capsule) and control group (155 cases, taking placebo capsule). The results showed that in the study group, there were 161 cases (95.8%) of complete abortion, 7 cases (4.2%) of incomplete abortion; In the control group, there were 146 cases (94.2%) of complete abortion, 6 cases (3.9%) of incomplete abortion, 3 cases (1.9%) of abortion failure. The vaginal bleeding time was 5-25 days (mean 10.8 days) in study group, while that was 6-62 days (mean 19.1 days) in control group. The menstrual cycle was 30.5+/-5. 2 days and 33.8 d+/-8.6 days respectively in study and control groups. The menstrual period was 6.1+/-3. 5 days and 9.9+/-5.1 days respectively in study and control groups. Yaoliuan capsule is an effective drug to prevent and treat vaginal bleeding following drug-induced abortion, promote menstruation recovery and prevent pelvic infection.
Abortifacient Agents, Nonsteroidal/adverse effects ; Abortion, Induced/*adverse effects ; Capsules ; Drugs, Chinese Herbal/*therapeutic use ; Menstruation/*drug effects ; Phytotherapy ; Pregnancy Trimester, First ; Uterine Hemorrhage/etiology ; Uterine Hemorrhage/*prevention & control