1.Expression and significance of interleukin-18.12 and tumor necrosis factor-α in intrahepatic cholestasis of pregnancy
Chinese Journal of Obstetrics and Gynecology 2011;46(5):329-332
Objective To investigate the effect of Interleukin(IL)-18,IL-12 and tumor necrosis factor-α(TNF-α)in hepatic injury in intrahepatic cholestasis of pregnancy(ICP).Methods Sixty-two cases of ICP patients(ICP group),30 cases of normal pregnant women(control group)and 30 cases of hepatitis B(HBV) women (hepatitis group) were recruited. Serum IL-18, IL-12 and TNF-α were examined by ELISA. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were examined by automatic biochemical analysis instrument. Results ( 1 ) In hepatitis group, serum concentrations of IL-18,IL-12 and TNF-α were (256±51 ) ng/L, ( 122±96) ng/L and (207±3) ng/L; serum levels of ALT and AST were(363±174) U/L and (359 ±237) U/L, respectively. In ICP group, serum concentrations of IL18, IL-12 and TNF-α were (72±32) ng/L, (42 ±28) ng/L and (48±14) ng/L; serum levels of ALT and AST were (201 ±128) U/L and ( 132±87) U/L, respectively. While in control group, serum concentrations of IL-18, IL-12 and TNF-α were (43 ± 13) ng/L, ( 10±3) ng/L and (33±9) ng/L; serum levels of ALT and AST were (13 ~ 4) U/L and (15 ± 3) U/L, respectively. Serum IL-18, IL-12, TNF-α, ALT and AST levels in hepatitis group were significantly higher than those in ICP group and control group ( P <0. 05 ).Serum IL-18, IL-12, TNF-α, ALT and AST levels in ICP group were significantly higher than those in control group(P < 0. 05 ). (2) In severe ICP subgroup, serum concentrations of IL-18, IL-12 and TNF-α were (81 ±32) ng/L, (50 ±25) ng/L and(50 ± 14) ng/L; serum levels of ALT and AST were (269 ± 111 ) U/L and (181±73) U/L In mild ICP subgroup, serum concentrations of IL-18, IL-12 and TNF-α were (48 ±18 ) ng/L, (17 ± 4 ) ng/L and (40 ± 10 ) ng/L; serum levels of ALT and AST were (87±46) U/L and (50 ±21 ) U/L, respectively. Serum IL-18, IL-12, TNF-α, ALT and AST levels in severe ICP subgroup were significantly higher than those in mild ICP subgroup and control group (P < 0. 05). And serum ALT and AST levels in mild ICP subgroup were significantly higher than those in control group(P <0. 05). (3) There were 16 cases with preterm birth (50%, 16/32 ) and 10 cases with meconium-stained amniotic fluid( 31%, 10/32 ) in severe ICP subgroup, significantly higher than those in mild ICP subgroup ( P< 0. 05 ), which contained 2 preterm births ( 7%, 2/30) and 1 meconium-stained amniotic fluid (3%, 1/30). While in control group, the numbers were 1(3%, 1/30)and 1(3%, 1/30),respectively. As for the cases of neonates whose 1 minute Apgar score were not more than 7, there were 2 cases, 1 case and 1 case in severe ICP subgroup, mild ICP subgroup and control group, respectively,which showed no significant difference(P> 0. 05). Conclusion Serum IL-18, IL-12 and TNF-α may be involved in the process of hepatic injury of ICP.
2.Status and trends of group counseling researches from 1996 to 2013 in China
Chinese Mental Health Journal 2015;(4):258-263
Objective:To investigate the current status and trends of group counseling research in China by bibliometric analysis and content analysis.Method:Using methods of bibliometrics and content analysis,Chinese papers on group counseling in 1996 -2013 which were found in CNKI were analyzed in 6 aspects including litera-ture number,sources,research type,topic,quality and competence of group counselors.Results:A total number of 516 papers were found,in which 51.0% of journal articles and 49.0% of dissertations.The research methods and topics were developing from outcome and quantitative research to multiple methods and topics including process re-search,process-outcome research,qualitative research,combined research and theoretical research.The problems were that researchers were still focused on outcome research (92.6%)and quantitative research (54.5%).As to research quality,40.7% of journal articles were published on CSSCI,22.5% of the experimental studies had no control groups,among which 43.3% were not randomized or matched,and 75.5% of outcome researches had not traced long-term effects.In 65.4% of the researches,group leaders'qualifications were not described.Conclusion:The group counseling research in China is developed quite rapidly,and the research type and topic are more di-verse,but the quality of the studies needs to be improved,and the competence of group leaders needs to be exam-ined.
3. Determination of five saponins in Ginseng Radix et Rhizoma-Bupleuri Radix herb pair and its preparations by QAMS method
Chinese Traditional and Herbal Drugs 2018;49(12):2873-2877
Objective To study the effects of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, saikosaponin a, and saikosaponin d in Ginseng Radix et Rhizoma-Bupleuri Radix (GRRBR) herb pair and its preparations by using quantitative analysis of multi-components by single-marker (QAMS). Methods On the basis of ginsenoside Rg1, the relative correction factors between the ginsenoside Rg1 and the other four saponins were established, and then the contents of the other four saponins were calculated. At the same time, the contents of the five components were determined by external standard method and compared with those evaluated by QAMS. The relative retention time was determined by different chromatographic columns. It could be considered that QAMS was feasible and accurate in the determination of saponins in GRRBR herb pair. Results A quantitative control method of five kinds of saponins was established, and the methodological results were good. The results showed that there was no significant difference in the contents of five kinds of saponins in GRRBR herb pair, Xiaochaihu Decoction, and Kaixin Jieyu Prescription between QAMS group and external standard method group. Conclusion QAMS is suitable for the determination of saponin in GRRBR herb pair, which can be used as a reference for the determination of its effective components and the establishment of compound quality control method.
4.Effect Evaluation of Teachers' Scientific Research Ability Cultivating in Medical Vocational Colleges
Lucai SHAO ; Yuzhong JIN ; Ying LIU
Chinese Journal of Medical Science Research Management 2016;29(2):132-134
Objective The paper is to explore the cultivation effect of teachers' scientific research ability in medical vocational college.Methods The proportional stratified sampling method was introduced.108 professional teachers were selected as the research objects in Cangzhou Medical College.The various measures were taken to cultivate teachers' scientific research ability.Comprehensive evaluation of teachers' scientific research ability is divided into subjective and objective quantitative evaluation.The results of the pre-test and post-test were compared.Results After the implementation of cultivation, scientific research ability of teachers significantly increased in medical vocational college.Before and after the cultivation, the total score of comprehensive evaluation had significant difference (P<0.01).The total score of subjective and objective quantitative evaluation had significant difference (P<0.01).Conclusions The cultivating measures are actively adopted in medical vocational colleges.It has a positive impact on teachers' scientific research ability.
5.Study on Optimum Conditions of Isolation and Purification of Specific Toxin Fractions Produced by Exserohilum turcicum
Shao-Xin WANG ; Jin-Gao DONG ;
Microbiology 1992;0(02):-
Five fractions,Ⅰ(R_f0.06)、Ⅱ(R_f0.21)、Ⅲ(R_f0.45)、Ⅳ(R_f0.60)、Ⅴ(R_f0.75)have been obtained after HT-toxin from race 1 of Exserohilum turcicum 99-2 isolated by TLC. In all of these fractions,only fractions Ⅱ had specific toxicity to the corn leaves with Ht1 gene. Then fractions Ⅱ-1、Ⅱ-2、Ⅱ-3 were isolated from fractions Ⅱ by HPLC purification,and the bioassay result showed only fraction Ⅱ-3 was toxigentic to corn leaves with Ht1 gene but non-toxigentic to corn leaves without Ht1 gene. Fractions Ⅱ-1、Ⅱ-2 and Ⅱ-3 were scanned by UV-Vis spectrophotometer. It was shown that the fractions Ⅱ-1 and Ⅱ-2 had analogous spectrum,and especially the fraction Ⅱ-3 had a special peak at 300nm.
6.Microsurgical anatomic study of subtemporal approach using a minimal access
Xuefei SHAO ; Liangwei WANG ; Jin TAO
Chinese Journal of Microsurgery 2013;36(4):360-363
Objective To investigate the surgical exposure of the subtemporal approach and explore clinical indications using a minimal-access.Methods Ten adult cadaveric heads fixed with formalin were used in this study.The holes with 3.0 cm × 2.5 cm of zygomatic arch vertically were operated on each head with subtemporal approach.During the anatomical procedures,measured the maximal exposure lengths of tentorial margin,posterior cerebral artery,and anterior border of brain stem,vertical distances between highest structure in the field of view and posterior clinoid process,the shortest distances form the zygomatic arch 1/3 to tentorial edge,sulcus lateralis mesencephali and anterior clinoid process; After tentorium of cerebellum was cut,measured the straight distance form the internal carotid artery to the optic nerve and form the posterior communicating artery to the tentorial edge.Results Oculomotor,trochlear nerve,tentorial edge,superior cerebllar artery.,P1-P2 segment of posterior cerebral artery,ventrolateral surface of mesencephalon and pon higher than root of trigeminal nerve,anterior and posterior clinoid process,posterior communicating artery and anterior choroidal artery,and superior portion of pituitary stalk could be observed via subtemporal approach using a minimal-access.Conclusion 1.The subtemporal approach using a minimal-access can protect the superficial temporal artery and the facial nerve branches especially in the process of the flap formation.It does little damage to the temporallis,reduces the invalid exposure of brain tissue,farthest lowers the damage to the scalp,skull and adjacent tissue ; 2.The subtemporal approach using a minimal-access can obtain the exposure rang.It can see the upper pons,petroclival region,tentorial notch area,ventrolateral brain stem.
7.Clinical Study on Acupuncture Treatment of Dysphasia in 80 Hypophrenia Children
Quanming ZHANG ; Rui JIN ; Yang SHAO
Journal of Acupuncture and Tuina Science 2006;4(3):156-158
Objective: Investigate the curative effect of acupuncture on dysphasia in hypophrenia children. Methods: One hundred and sixty children with hypophrenia and dysphasia were randomly divided into an acupuncture group of 80 cases, a language training group of 40 cases and a composite group of 40 cases for clinical observation. Total intelligence quotient (TIQ), vocal intelligence quotient (VIQ) and practical intelligence quotient (PIQ) were compared between pretreatment and posttreatment in the children themselves and between the groups before and after treatment. Results: The total efficacy rate was 77.5% in the acupuncture group, 82.5% in the composite group and 52.5% in the language training group. Conclusion: Acupuncture significantly raises TIQ, VIQ and PIQ in the sick children. Language training only raises VIQ to some degree.Acupuncture in cooperation with language training is an effective way to treat this disease.
8.The study of P-wave duration and P-wave dispersion for the prediction of idiopathic proxysmal atrial fibrillation and intervention by amiodrone
Weidong JIN ; Bochu SHAO ; Yimin WANG
Chinese Journal of Practical Internal Medicine 2002;0(08):-
Objective To assess the prediction value of Pmax and Pd on idiopathic paroxysmal atrial fibrillation(IPAF) and the possible mechanism of amiodrone preventing the recurrence of IPAF.Methods Pmax and Pd were measured in 60 patients with IPAF and 60 healthy control subjects by 12-lead surface electrocardiography. 30 of patients with IPAF were assigned to treatment with amiodrone for 6 months. Pd?Pmax and recurrence of IPAF were observed and compared with control group during the treatment.Results Pmax and Pd were found to be significantly higher in patients with IPAF than in healthy subjects[(126?17)ms vs(102?11)ms; (50?9)vs(29?8)ms,P
9.Effect of acupoint transcutaneus electrical nerve stimulation(ATENS) on stereoacuity in amblyopic children patients
Xiaofang YANG ; Jin CUI ; Wanfu SHAO
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(01):-
Objective: To observe the effect of ATENS on distance and near vision and stereopsis in amblyopic children patients.Methods: 80 eyes from 70 patients with amblyopia were randomly divided into two groups: the group of ATENS and the group of western medicine.The difference of distance,near vision and stereopsis before and after treatment and between two groups after treatment were observed.Results: Distance and near vision improved and stereopsis diminished significantly after treatment in the TENS group and the western medicine group(P0.05).The curative effect and improving stereopsis in the western medicine group were better than that in the TENS group(P
10.Intravenous infusion of potassium chloride at different concentrations for correction of hypokalemia during perioperation: an experimental study
Xinli SHAO ; Xian KUANG ; Shiao JIN
Chinese Journal of Anesthesiology 1994;0(06):-
A certain amount of potassium chloride is required to correct the hypokalemia occuring commonly during perioperation,but the overdosage may be harmful. To investigate the proper dose of KCl,40 adult rabbits were randomly and evenly assigned to be intravenously infused with normal saline(group Ⅰ),or saline containing 4,25 or 50 mmol/L KCI (group Ⅱ,Ⅲ,Ⅳ),at rate of 10 ml?kg~(-1)?h~(-1) during operation respectively. As compared with those before operation,the serum level of potassium decreased significantly in group Ⅰ and Ⅱ, changed unsignificantly in group Ⅲ,and increased in group Ⅳ during and after operation;the potassium amounts in liver and skeletal muscles increased markedly after operation. S-T segment was depressed in group Ⅰ and Ⅱ,T wave became peaked in group Ⅳ, and ECG remained normal in group Ⅲ. it is indicated that intravenous KCl infusion at concentration 25 mmol/L and rate of 10ml?kg~(-1)?h~(-1) may properly correct the hypokalemia during perioperation,and the occurance of perioperatine hypokelemia may be related to the uptake of liver and skeletal muscles