[Objective] To discuss the therapeutic effect of different methods in the treatment of intertrochanteric femur fracture. [Methods]Totally 118 middle aged or old patients with intertrochanteric femur fracture were fixed with dynamic hip screw (DHS), Gamma nail or PFNA.[Results]Totally 112 cases were followed up, and the average time was 16 months, in those 45 patients were fixed with DHS: 29 were excellent, 11 cases were good, and 5 were poor, and the excellent to good rate was 88.9%; 36 patients were treated with Gamma nail, and the excellent, good, poor results were 28, 2, 1, respectively, and the excellent to good rate was 96.8%; 36 patients were fixed with PFNA, and the excellent, good, poor results were 29, 6, 1, respectively. The differences were significant (P=0.02, analyzed by Logistic regression with SPSS software). The excellent and good superiority of Gamma nail and PFNA were 5.1552 and 2.3726 times compared to DHS, and there was significant differences between the groups.[Conclusion]Surgical treatment of intertrochanteric femur fracture can improve the functional recovery and reduce the complication incidence, and PFNA fixing method can shorten the operative time, reduce the bleeding and the therapeutic effect is reliable, and suitable for old patients combined with osteoporosis.

0.9 mm(n = 26).Twenty-five healthy individuals served as a control group.Clinical data including age,sex,BMI,ABI,HbA1c,fasting glucose and insulin were collected.Insulin resistance index in Homa model was calculated following the equation:Homa IR = FINS ? FPG) /22.5.Serum adiponectin level was measured by ELISA.Results The serum adiponectin level was significantly lower in atherosclerosis group than in non-atherosclerosis and control groups(P

OBJECTIVE:To establish a simple method for calculating the optimal pharmacokinetic-equation parameters in drugs by intravenous administration using Excel programming solution.METHODS:The pharmacokinetic parameters and compartment model parameters after intravenous injection drugs were computed by means of Excel programming solution,and the results were compared with those computed by DAS method or residual method.RESULTS:By means of Excel programming solution,the optimal pharmacokinetic parameters of one-and two-compartment models were obtained,which were completely in line with those computed by DAS program.CONCLUSION:Excel programming solution is applicable for the computation of the pharmacokinetic parameters of one-and two-compartment models following intravenous injection drugs.

OBJECTIVE:To establish a simple individualized dosage regimen of multiple oral dosing of Theophyllin. METHODS:Based on pharmacokinetic parameters,Excel function was used to design the dosage regimen of multiple oral dosing extravascular administration of one-compartment model with Theophylline as an example. RESULTS:The following parameters such as plasma drug concentration at any time (t) since administration of Theophylline,peak time,maximum steady plasma-drug concentration,minimum steady plasma-drug concentration,accumulation coefficient,fluctuation percentage,fluctuation amplitude,and dosage for children and the old could be obtained and the concentration-time curves were able to be drawn from the input data including physical and pathological parameters,dosage (X0) of Theophylline,interval ?,absorption rate constant (Ka),drug clearance rate (CL),absorption fraction (F) and apparent volume of distribution (Vd). CONCLUSION:The method adopted in our study is simple,reliable and intuitive,and it is applicable for the design of the individualized dosage regimen of Theophyllin.

AIM: To study the application of superfine comminution in Renzhujianwei Granule. METHODS: After being pretreated, Rhizoma Altractylodis macroleplea and coix seed were crushed into ultra-fine powder by TC20 medical superfine crusher. Then the ultra-fine powder was made into the granule. RESULTS: Detected by scanning electron microscope and EDS X-ray detector, the mean diameter of the particle was ≤5 ?m; The detection of the normal distribution was 87.93%≤20 ?m and 95.08%≤20 ?m. CONCLUSION: The application of ultra-fine powder in Renzhujianwei Granule develops a new method of producing granule, that can save the pharmaceatic adjuvants and keep the effective components, and is propitious to the molding of the preparation, and also can improve the stability.

ObjectiveTo study the single nucleotide polymorphisms(SNPs) in the ORF26 gene of HHV-8 in Kaposi's sarcoma(KS),and to assess their correlations with the clinical phenotype and mucosal invasion of KS.MethodsHHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from paraffin-embedded tissue specimens obtained from 32 cases of KS(including 26 classic and 6 AIDS-related KS).The ORF26 gene of HHV-8 was amplified by nested-PCR followed by bidirectional sequencing.The software DNAStar and program Clustal W were used to assess the SNPs in the ORF26 gene.Statistical analysis was carried out by using the Fisher's exact probability test.ResultsHHV-8 DNA was detected in 30 of the 32 tissue specimens,and in all of the 6 AIDS-related specimens.The predominant SNPs were 981 T/C(n =12),1086 C/T(n =12) and 1139 A/C(n =12) in the ORF26 gene of the 30 strains of HHV-8.No significant difference was observed in the distribution of SNPs in ORF26 between different phenotypes of KS or between KS with and without mucosal invasion.ConclusionThe ORF26 SNPs of HHV-8 seem unrelated to the clinical phenotypes or mucosal invasion of KS.

Objective To observe curative and side effects of fluconazole in preventing and treating fungal infection in patients with acute leukemia.Methods 112 cases patients with acute leukemia were divided into two groups.Group A was prevented group (25 cases).These patients had fluconazole by oral administration with 100 mg/d for 7 days when the peripheral white blood cell were less than 2.0×109/L.Group B was treated group (87 cases).These patients had intravenous drip fluconazole with 200 mg/d if they reach the fungal infection diagnosis standard.To observe the characters of fungal infection in acute leukemia,the occurrence ratio of fungal infection in these two groups and the side effects of fluconazole.Results The occurrence ratio of fungal infection in Group A and B had obvious difference.(χ2=4.21,P<0.05);Patients with acute leukemia were easily infected by fungal in gastrointestinal,respiratory and urinary systems.There is no sereve side effect observed in using fluconazole.Conclusion:Taking fluconazole by oral administration can safely and effectively prevent fungal infection in patients with acute leukemia.

OBJECTIVETo investigate molding technology of total alkaloids from Sophora alopecuroides freeze-dried powders and observe its inhibition effects on liver transplantation tumor in mice.

METHODWith color, clarity, water-soluble and formability as indexes, single factor tests were adopted to screen type and amount of filler, the concentration of total alkaloids in drug liquid, pH in order to determine optimum prescription of total alkaloids from S. alopecuroides freeze-dried powders, the lowest melting point was determined and freeze drying curve was drafted. Mice hepatoma H22 ascites tumor strain was sterile inoculated in right axillary subcutaneous of mice, and antitumor effect of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice.

RESULTWhen selected 80 g x L(-1) as mannitol as filler, the concentration of total alkaloids in drug liquid was 25 g x L(-1) and pH 6.5-7.5, freeze-dried effect was optimum with fast reconstitute speed. Average inhibition rate of the big (120 mg x kg(-1)) and medium (60 mg x kg(-1)) dose group of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice were 56.08% and 35.49%, respectively.

CONCLUSIONPreparation technology was reasonable, reproducible and stable, total alkaloids from S. alopecuroides freeze-dried powders had significant antitumor effect and showed a dose-effect relationship.

Alkaloids ; chemistry ; pharmacology ; Animals ; Antineoplastic Agents, Phytogenic ; chemistry ; pharmacology ; Cell Line, Tumor ; Color ; Freeze Drying ; Humans ; Liver Neoplasms ; drug therapy ; Mice ; Powders ; chemistry ; Sophora ; chemistry ; Tumor Burden ; drug effects

Acute Coronary Syndrome ; Humans ; Platelet Aggregation Inhibitors

OBJECTIVE:To provide reference for implementing relevant policies and measures,and promote rational drug use. METHODS:The cognition(questionnaire)and application(prescription spot checks)about antibiotics and glucocorticoids by 191 rural doctors from 14 township hospitals and 18 health room in area A,B and C(representing the economically developed areas, moderately developed areas and undeveloped areas)were investigated,and the results were statistically analyzed. RESULTS:Total-ly 382 questionnaires were sent out(191 for antibiotics and 191 for glucocorticoids),and 382 were effectively received with effec-tive recovery of 100%;and 2 300 prescriptions were checked. Results of questionnaire showed,there were still some misunder-standings and blindness among the rural doctors'cognition and application about antibiotics and glucocorticoids,the usage of β-lac-tam was not fully grasped,part of the empirical use of antibiotics and drug treatment was still not entirely clear,more than 40%had no clear ideas about the classification and contraindications of hormones,and more than half of them didn't know the Glucocor-ticoids Clinical Practice Guidelines;results of prescription spot checks showed,there were regional differences about the proportion of antibiotics and glucocorticoids,area A had the highest proportion among 3 areas,accounting for 60.9% and 18.9%,respective-ly. And area C showed highest proportion about unreasonable prescriptions,accounting for 22.5%. CONCLUSIONS:The cognition and reasonable use of rural doctors for antibiotics and glucocorticoids still needs to be strengthened. It is suggested to take effective measures,formulate and implement the corresponding supervision system,strengthen antibiotics and glucocorticoids knowledge training for rural doctors and fully implement the the principles of rational drug use to improve the rational drug use.