Aim To investigate the effects of xy9902 on the proliferation and differentiation in MC3T3-E1 cells and its mechanism.Methods Effects of xy9902 on the proliferation and differentiation of MC3T3-E1 cells was determined by MTT and ALP activity measurement.Competitive estrogen-receptor binding assay was used to investigate the affinity of xy9902 to ER.Results xy9902 induced cell proliferation and increased alkaline phosphatase(ALP) activity of MC3T3-E1 cells.Antiestrogen tamoxifen eliminated the stimulation of proliferation and ALP activity of MC3T3-E1 induced by xy9902.The relative binding affinity of xy9902 for ER? and ER? showed an IC_(50)of 8.45?10~(-6) mol?L~(-1) and 1.66?10~(-6) mol?L~(-1) respectively.Conclusion xy9902 directly stimulates cell proliferation and differentiation of MC3T3-E1 cells through ligand-receptor binding,which indicated that xy9902 behaves as an estrogen agonist in MC3T3-E1 cells.

Objective To investigate the influences of Rhodospirillaceae on immune function of mice.Methods Four tests were performed in mice after 4 w medication with different doses of Rhodospirillaceae ig,including:1.The transformation of spleen lymphocyte(MTT method);2.The phagocytosis of macrophagocyte(semi-vivo method);3.The value of HC50; 4.The activity of NK cell(LDH method).Results1.The transformation of spleen lymphocyte;2.The phagocytosis of macrophagocyte,the Value of HC50 and the activity of NK cell in test groups were all raised,the differences were significant and very significant when compared with the control group.Couclusions Rhodospirillaceae could enhance the cellular and humoral immunity of mice.

The differential diagnosis of pancreatic head mass has been clinical difficulties.Endoscopic ultrasonography is now recognized as one of the sensitive and effective methods for the diagnosis of pancreatic diseases.This paper discusses the diagnostic performance of endo-scopic ultrasonography for various common types of pancreatic head mass,as well as the radiological features of this disease,reviews the di-agnostic value and safety of endoscopic ultrasonography-guided fine needle aspiration for pancreatic head mass,and briefly introduces the advances in the application of additional new techniques of endoscopic ultrasonography for this disease.Finally,it is pointed out that endo-scopic ultrasonography has a high diagnostic value for pancreatic head mass.

With the development of endoscopic techniques,the recent emergence of additional techniques of endoscopic ultrasound (EUS) have provided a novel means for the diagnosis and treatment of pancreatic tumors,which has received more and more attention and recogni-tion from around the world.This article reviews the progress in additional new techniques of EUS in the treatment of common pancreatic tumors.Finally,we can conclude that the additional techniques of EUS have high therapeutic values for pancreatic tumors.

Objective To research the way ro deal with the acute laryngeal damage.Methods Data of 23 cases with acute laryngeal damage were analyzed.Results Among 23 cases,there were 6 cases who received non-surgical treatment and their phonation were normal except one case who had mild hoarseness.Among 17 cases who had been performed operation,13 cases had normal phonation after operation.4 cases still had roughness of voice.There were 15 cases with normal respiration but two cases with larynostenosis and difficulty in decannulation.And these two cases were decannulated respectively after 2 months and 3 years after operation.15 cases made of 80% in all were cured and decannulated within 1 month.Conclusion Acute laryngeal damage patients should be diagnosed and treated as early as possible according to the damage degree so as to recover the laryngeal and tracheals function and prevent the sequelae and increase the cure rate.

Objective To study the clinical features,diagnosis and management of nose and nasal sinusen fungus.Methods Different manner of surgical procedure was taken according to the areas and degree of pathological changes.The patients with noninvasive fungus were treated with radical maxillary sinusotomy(cald-well-Luc operation) or endoscopic sinus surgery.The patients with invasive form were undergone by the sinusotomy of enlarging areas,and combined with a postoperatively antifungal therapy.Results Twenty-six patients were involved in noninvasive fungus,and 25 cases in them were cured in single surgery through different manner.Nine patients were in invasive one,and 7 in them were also cured by single surgery in dfferent manner.One patient with invasivi fungus died 5 months postoperatively for extensive area and noneffective treatment although four surgical excisions were done.Conclusion CT scan is very important in their diagnosis,and the histopathology leads to identification of the fungus.Surgical removal is a radical kind of treatment.It is essential to exercise and open entirely the involved sinus cavity whatever type,and to enlarge areas for the extensive invasive fungus.

Hepatitis B ; pathology ; therapy ; Hepatitis B Surface Antigens ; Hepatitis B virus ; Humans

Objective To investigate the relationship between the p27~(kip1) expression and the change of radiosensitivity of esophageal cancer cells.Methods Radio-resistant cells (EC9706R) were gradually isolated by means of repeated gamma-ray irradiation (6 000 cGy in total) upon human squamous esophageal carcinoma cells (EC9706).The radiosensitivity of these two types of cells was measured by standard colony formation assays,their cell cycle distribution analyzed by flow cytometry respectively,and the p27~(kip1) expression detected by immunocytochemistry.Results As compared with their parent cells,the isolated human squamous esophageal carcinoma cells(EC9706) showed clearly greater radio-resistance(for EC9706R,SF_2=65.71%,D_0=2.20 Gy,D_q=1.61 Gy,N=2.07,and for EC9706,SF_2=46.72％,D_0=1.61 Gy,D_q=0.99 Gy,N=1.85;SF_2,D_0,Dq and N were all higher).As to the cell cycle distribution,the population of G_1 phase cells in EC9706R cells was significantly decreased,but the proportion of S phase cells was significantly increased as compared with the parent cells.Immunocytochemistry revealed that the p27~(kip1) expression of EC9706R cells was clearly lower than that of EC9706 cells.Conclusion Cell phase change due to the decrease of p27~(kip1) expression may be one of the mechanisms of the generation of radio-resistance in esophageal cancer cells.

Objective To investigate the effect of peginterferon alfa-2a injection (PEG-IFNα-2a) combined with ribavirin (RBV) in the treatment of chronic hepatitis C virus (1b) in patients with chronic hepatitis C. Methods The clinical data of patients with type 1b chronic hepatitis C in our hospital from May 2010 to October 2015 were retrospectively analyzed. According to the method of treatment, the patients were divided into IFNα-2a combined with RBV group and PEG-IFNα-2a combined with RBV group. The rapid virologic response (RVR), early virologic response (EVR), and sustained virologic response (SVR) rate were observed in the two groups. The liver function, expressions of CD4+T and CD8+T cell of peripheral blood and the incidence of adverse reaction were compared between the two groups before and after treatment. Results The RVR, EVR, SVR of two groups was no significant difference (χ2=0.641, 0.946, 0.154, P=0.423, 0.331, 0.694). After treatment, the levels of ALT , AST, DBIL and TBIL in PEG-IFNα-2a combined with RBV group were lower than those in IFNα-2a combined with RBV group (P<0.05). Of the PEG-IFNα-2a combined with RBV group, the CD4+T level was lower and the CD8+T level was higher than that in PEG-IFNα-2a in the combined Leigh Bhave Lin group were lower than that in the IFNα-2a level was lower IFNα-2a combined with RBV group(P<0.05). There was no significant difference in the incidence of influenza-like symptoms, marrow suppression, somnolence and abnormal laboratory indexes between two groups. Conclusion PEG-IFN alpha -2a combined with RBV has a good therapeutic effect on type 1b chronic hepatitis C virus, and has good safety and clinical application value.

Objective To investigate the changes of antibiotic resistance and its correlations with antibiotics use density (AUD) in intensive care unit (ICU) after implementation of antimicrobial stewardship. Methods Data on AUDs and synchronous antibiotic resistance in ICU of the Affiliated Hospital of Taishan Medical University during 2010 and 2014 were retrospectively reviewed.Autoregressive integrated moving average ( ARIMA) model was used to identify the correlation between AUDs and antibiotic resistance.Results The total AUD which stood at 163.46 DDDs· 100 person-1 · d-1 in the year 2010 decreased to 68.20 DDDs · 100 person-1 · d-1 in 2014, and the most significant decreases were found in quinolones, imidazole, carbapenems, aminoglycosides, other beta lactam and macrolides. Synchronously, a wide range of improvements in antibiotic resistance of major pathogens were also observed from 2010 to 2014, and the improvements were positively correlated with the decrease of AUDs.However, antibiotic resistance in 2014 was not significantly improved compared with that in 2013. The rate of ciprofloxacin resistance in Pseudomonas aeruginosa and the resistant rates of amikacin, imipenem, and meropenem in Acinetobacter baumannii even increased in 2014.Conclusions With the implementation of antimicrobial stewardship, antibiotic consumption in ICU was decreasing, and antibiotic resistance rate in major pathogenic bacteria was decreasing.But the rebound in antibiotic resistance in some types of bacteria was also observed and should be put on enough caution.